Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,317,576 G→T 5.9% A144E (GCG→GAG)  rimP ← ribosome maturation factor for 30S subunits

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,317,5760GT 5.9% 747.2 / 24.6 205A144E (GCG→GAG) rimPribosome maturation factor for 30S subunits
Reads supporting (aligned to +/- strand):  ref base G (95/98);  new base T (7/5);  total (102/103)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

Only 100 of 205 total aligned reads displayed.

GTCGCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCAGAGTCACCTCTTCTCCGA  >  NC_000913/3317429‑3317723
                                                                                                                                                   |                                                                                                                                                   
   gCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTc                                                                                                                                                >  2:1033004/1‑150 (MQ=255)
   gCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTc                                                                                                                                                >  2:2349956/1‑150 (MQ=255)
    ccAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCt                                                                                                                                               <  2:130962/150‑1 (MQ=255)
       gcgcgcTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGa                                                                                                                                            <  2:1534571/150‑1 (MQ=255)
       gcgcgcTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGa                                                                                                                                            <  2:2635322/150‑1 (MQ=255)
         gcgcTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGata                                                                                                                                          <  2:1335036/150‑1 (MQ=255)
           gcTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATAtt                                                                                                                                        >  1:2655733/1‑150 (MQ=255)
            ctgtCCACTGCTTCGCAAATCTTCTCGCGAGGGAGCGCCTTTTCTTTGGATACGTCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCATTTCACCTCATCCCGACTATTTATAGTGGGGTACCAGGTTCGCCTTCTGGATATTa                                                                                                                                       <  2:395092/147‑1 (MQ=255)
            ctgtCCACTGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGACTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTa                                                                                                                                       <  2:2441014/147‑1 (MQ=255)
             tttCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGACCCAGGTTCTCCTTCTGGATATTAc                                                                                                                                      >  1:795525/1‑150 (MQ=255)
             tttCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCTCCTTCTGGATATTAc                                                                                                                                      >  2:1649395/1‑150 (MQ=255)
             tttCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTAc                                                                                                                                      >  2:858381/1‑150 (MQ=255)
             tttCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTAc                                                                                                                                      >  2:2277161/1‑150 (MQ=255)
               tCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTc                                                                                                                                    >  1:1408121/1‑150 (MQ=255)
                   aTGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAgcg                                                                                                                                <  1:450315/150‑1 (MQ=255)
                    tGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAgcgc                                                                                                                               >  1:1046045/1‑150 (MQ=255)
                    tGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAgcgc                                                                                                                               >  2:1819357/1‑150 (MQ=255)
                       ttCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGaa                                                                                                                            >  2:61152/1‑150 (MQ=255)
                          gAAAATCTTCTCGCGAGGTCGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGGTCGCCTTCTGGATATTACTCAGCGCGAacac                                                                                                                         >  2:1481172/1‑150 (MQ=255)
                          gAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAacac                                                                                                                         >  1:1338843/1‑150 (MQ=255)
                          gAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAacac                                                                                                                         >  1:1925974/1‑150 (MQ=255)
                           aaaaTCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACt                                                                                                                        >  2:1027577/1‑150 (MQ=255)
                           aaaaTCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACt                                                                                                                        >  1:2579635/1‑150 (MQ=255)
                             aaTCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTc                                                                                                                      <  1:2350851/150‑1 (MQ=255)
                              aTCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCa                                                                                                                     <  2:2215044/150‑1 (MQ=255)
                                  tctcGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCTCCTTCTGGATATTACTCAGCGCGAACACTTCATCtt                                                                                                                 >  1:122766/1‑150 (MQ=255)
                                   ctcGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCttt                                                                                                                <  1:2869812/150‑1 (MQ=255)
                                          ggTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCg                                                                                                         <  2:89383/150‑1 (MQ=255)
                                          ggTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCg                                                                                                         <  2:585939/150‑1 (MQ=255)
                                           gTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCTCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGa                                                                                                        <  2:2261931/150‑1 (MQ=255)
                                           gTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGa                                                                                                        <  2:1852798/150‑1 (MQ=255)
                                           gTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGa                                                                                                        <  2:1390367/150‑1 (MQ=255)
                                                gcCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTa                                                                                                   >  1:2531162/1‑150 (MQ=255)
                                                   ttttCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACt                                                                                                <  1:1290003/150‑1 (MQ=255)
                                                   ttttCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACt                                                                                                <  2:529344/150‑1 (MQ=255)
                                                        aTTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGAt                                                                                           <  1:532127/150‑1 (MQ=255)
                                                         ttGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATc                                                                                          >  1:82465/1‑150 (MQ=255)
                                                          tGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCTCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCa                                                                                         >  2:2307263/1‑150 (MQ=255)
                                                            gATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCAtt                                                                                       >  2:1707814/1‑150 (MQ=255)
                                                              tACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTc                                                                                     <  1:83666/150‑1 (MQ=255)
                                                               aCGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCa                                                                                    <  1:993264/150‑1 (MQ=255)
                                                                cGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCTCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCAc                                                                                   >  1:773043/1‑150 (MQ=255)
                                                                       aaCTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTAc                                                                            <  1:572644/150‑1 (MQ=255)
                                                                         cTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCg                                                                          <  1:2277161/150‑1 (MQ=255)
                                                                         cTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCg                                                                          <  1:858378/150‑1 (MQ=255)
                                                                            cAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCtt                                                                       <  1:61152/150‑1 (MQ=255)
                                                                             aGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCttt                                                                      <  1:2187787/150‑1 (MQ=255)
                                                                              gCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTg                                                                     >  2:1512079/1‑150 (MQ=255)
                                                                               ccAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGa                                                                    >  2:653247/1‑150 (MQ=255)
                                                                               ccAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGa                                                                    <  1:2237644/150‑1 (MQ=255)
                                                                               ccAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGa                                                                    <  1:693735/150‑1 (MQ=255)
                                                                               ccAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGa                                                                    >  1:1623477/1‑150 (MQ=255)
                                                                                cAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGAt                                                                   >  1:6650/1‑150 (MQ=255)
                                                                                    aTTTCTGTGTTCCTCGCGGGCTTTTCACCTCCGCCAGACTATTAACAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACg                                                               <  2:1214664/150‑1 (MQ=255)
                                                                                     tttCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGc                                                              <  2:2184425/150‑1 (MQ=255)
                                                                                      ttCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGcc                                                             >  2:1791839/1‑150 (MQ=255)
                                                                                        cTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCt                                                           <  1:2759655/150‑1 (MQ=255)
                                                                                         tttGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTg                                                          >  2:2152343/1‑150 (MQ=255)
                                                                                          ttGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGc                                                         >  2:548720/1‑150 (MQ=255)
                                                                                           tGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGcc                                                        >  1:1402964/1‑150 (MQ=255)
                                                                                                aTCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCTCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTa                                                   >  2:506127/1‑150 (MQ=255)
                                                                                                aTCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTa                                                   <  2:1925974/150‑1 (MQ=255)
                                                                                                aTCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTa                                                   <  2:2518049/150‑1 (MQ=255)
                                                                                                   gcgGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTacga                                                >  2:991443/1‑150 (MQ=255)
                                                                                                   gcgGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTacga                                                >  1:1500684/1‑150 (MQ=255)
                                                                                                    cgGGCTTTTCACCTCATCCAGACGATTAAAAGTGGGGAACCAGGTGCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTACGACGGTAACTGTGATCATTTCACCGTCTACCGCTGTGATAACGCCCTGCCCTTTacgac                                               >  1:2719430/1‑150 (MQ=255)
                                                                                                        cTTGTCACCTCATCCAGACTATTAACAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGtt                                           <  2:2295939/150‑1 (MQ=255)
                                                                                                         ttttCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGttt                                          >  1:1885664/1‑150 (MQ=255)
                                                                                                         ttttCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGttt                                          >  1:2494904/1‑150 (MQ=255)
                                                                                                         ttttCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGttt                                          >  1:345413/1‑150 (MQ=255)
                                                                                                              aCCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCTCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTAc                                     <  2:1127248/150‑1 (MQ=255)
                                                                                                               ccTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCTCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTAcc                                    <  2:1616810/150‑1 (MQ=255)
                                                                                                                cTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCg                                   <  2:1954938/150‑1 (MQ=255)
                                                                                                                cTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCg                                   <  2:1398108/150‑1 (MQ=255)
                                                                                                                cTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCg                                   <  2:689424/150‑1 (MQ=255)
                                                                                                                 tCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGc                                  >  1:1955227/1‑150 (MQ=255)
                                                                                                                 tCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGc                                  <  2:778613/150‑1 (MQ=255)
                                                                                                                 tCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGc                                  <  1:214952/150‑1 (MQ=255)
                                                                                                                   aTCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCa                                >  2:757470/1‑150 (MQ=255)
                                                                                                                     ccAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATa                              >  1:762987/1‑150 (MQ=255)
                                                                                                                      cAGACTCGTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTGACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATAc                             <  2:2398478/150‑1 (MQ=255)
                                                                                                                      cAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCATCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACAGCAATAc                             >  2:17502/1‑150 (MQ=255)
                                                                                                                      cAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCTAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTTATAACGCCCTGACATTTAAGACGGTTGTGTAcctaactcc                             >  2:1877041/1‑143 (MQ=255)
                                                                                                                      cAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATAc                             >  1:2899659/1‑150 (MQ=255)
                                                                                                                      cAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATAc                             >  1:1456908/1‑150 (MQ=255)
                                                                                                                       aGACTATAAAAAGTGGGGAACCAGGTACGACTTATGGATATTACTCAGCGCGAACACTTAATCTTTACAAAAGACGGTAACTGTGATCATTTCACCTTCTACCGCTTTGATAACGCACTGACATTTACGACGTTTTTGTAACGCAAAACg                            >  1:2608099/1‑150 (MQ=255)
                                                                                                                              tAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAAcc                     >  1:208199/1‑150 (MQ=255)
                                                                                                                               aaaaGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCa                    <  2:932645/150‑1 (MQ=255)
                                                                                                                                 aaGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCaga                  <  1:1008540/150‑1 (MQ=255)
                                                                                                                                 aaGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCaga                  <  1:2797725/150‑1 (MQ=255)
                                                                                                                                 aaGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCaga                  <  1:301502/150‑1 (MQ=255)
                                                                                                                                 aaGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCaga                  >  2:1692051/1‑150 (MQ=255)
                                                                                                                                      gggAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCAGAGTCAc             <  2:2848756/150‑1 (MQ=255)
                                                                                                                                       ggAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCAGAGTCAcc            <  2:947020/150‑1 (MQ=255)
                                                                                                                                         aaCCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCAGAGTCACctc          <  2:2753182/150‑1 (MQ=255)
                                                                                                                                               gTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCAGAGTCACCTCTTCTcc    <  1:1319790/150‑1 (MQ=255)
                                                                                                                                                 tCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCAGAGTCACCTCTTCTCCGa  <  1:1768987/150‑1 (MQ=255)
                                                                                                                                                 tCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCAGAGTCACCTCTTCTCCGa  <  1:159472/150‑1 (MQ=255)
                                                                                                                                                 tCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCAGAGTCACCTCTTCTCCGa  <  1:802077/150‑1 (MQ=255)
                                                                                                                                                 tCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCAGAGTCACCTCTTCTCCGa  <  2:2012268/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCGCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGGATATTACTCAGCGCGAACACTTCATCTTTACCTTCGACGGTAACTGTGATCATTTCACCGTCTACCGCTTTGATAACGCCCTGCCATTTACGACGGTTTTGTACCGCCATACGGAGAACCAGAGTCACCTCTTCTCCGA  >  NC_000913/3317429‑3317723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: