Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,596,873 Δ1 bp 7.1% intergenic (‑136/+32) ypfJ ← / ← purC putative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,596,8730C. 7.1% 312.4 / 17.4 99intergenic (‑136/+32)ypfJ/purCputative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase
Reads supporting (aligned to +/- strand):  ref base C (42/50);  new base . (4/3);  total (46/53)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

Only 100 of 101 total aligned reads displayed.

CTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAG  >  NC_000913/2596725‑2597021
                                                                                                                                                    |                                                                                                                                                     
cTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGcc                                                                                                                                                      >  1:236761/1‑150 (MQ=255)
cTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGcc                                                                                                                                                      <  1:1286283/150‑1 (MQ=255)
 ttGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCa                                                                                                                                                     <  2:118106/150‑1 (MQ=255)
   gCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGc                                                                                                                                                   <  1:485135/150‑1 (MQ=255)
    ccAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTTCCGTGCCAGCt                                                                                                                                                  >  1:411853/1‑150 (MQ=255)
    ccAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCt                                                                                                                                                  <  1:1544308/150‑1 (MQ=255)
    ccAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCt                                                                                                                                                  >  1:897428/1‑150 (MQ=255)
        cGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCAc                                                                                                                                             >  2:959536/1‑150 (MQ=255)
         gCATAGATACCTCAACAATATATTCATTATAGAAATCATCGTAGGTGGTGCAGAGGAAGATTACCACAGGAAAGCGGGTTGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACg                                                                                                                                            <  1:1094836/150‑1 (MQ=255)
          cATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACgg                                                                                                                                           <  2:205632/150‑1 (MQ=255)
           aTAGATAACTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGc                                                                                                                                          <  2:283187/150‑1 (MQ=255)
             aGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCaa                                                                                                                                        <  1:1212036/150‑1 (MQ=255)
               aTACCTCAACAATATATTCATTATAGAAATGATCGAACGTGGGTCAGAGGAAGATTACCACAGCAAAGCGGGATGAAAAGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAga                                                                                                                                      <  1:1051880/150‑1 (MQ=255)
                  ccTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTTCCCTGCCAGCTGCACGGCAAgatgt                                                                                                                                   >  1:610988/1‑149 (MQ=255)
                  ccTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatga                                                                                                                                   >  2:58279/1‑150 (MQ=255)
                  ccTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAgatga                                                                                                                                   >  1:1367345/1‑150 (MQ=255)
                       acaaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTTCCGTGCCAGCTGAACGGCAAGATGATTaaa                                                                                                                              >  1:394205/1‑149 (MQ=255)
                         aaTATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAg                                                                                                                            <  2:558432/150‑1 (MQ=255)
                            atatTCATTATAGAAATGATAGTAGGTGGTTCAGAGGAAGATTAACACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATAGTCAGAGAGCGGTTGTCTTTCCGTGCAAGCTGCAGGGAAAGATGATGAACAGaaa                                                                                                                         >  2:84569/1‑150 (MQ=255)
                             tatTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTg                                                                                                                                                <  2:288498/126‑1 (MQ=255)
                             tatTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTg                                                                                                                                                >  1:288498/1‑126 (MQ=255)
                              attaattaTAGAAATGATCTTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGTAATGAGCGTTAAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaaaaa                                                                                                                       <  1:1392892/150‑1 (MQ=255)
                              atTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGaaaaa                                                                                                                       >  2:915846/1‑150 (MQ=255)
                                tCATTATAGAAATGATCTTAGGTGGTTCAGAGGAAGATTACCACAGGAAATCGTGCTGAAATGGCGGAGTTATGAGCGTTCAATGCATAATATCCGAGAGCGGTTGTCTTGCCGGGCCAGCTGCACGGCAAGATGATGAACCGAAAAATc                                                                                                                     <  2:1419708/150‑1 (MQ=255)
                                 cATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCAGAGAGCGGTTGTCTTGCCGTGCCCGCTGCACGGCAAGATGATGAACAGAAAAATCa                                                                                                                    >  2:25908/1‑150 (MQ=255)
                                  aTTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAga                                                                                                                                      <  1:966265/131‑1 (MQ=255)
                                  aTTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAGCTGGCACGGCAAga                                                                                                                                      >  2:966265/1‑131 (MQ=255)
                                    tataGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTc                                                                                                                 <  1:233744/150‑1 (MQ=255)
                                      taGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCa                                                                                                               >  1:1621791/1‑150 (MQ=255)
                                       aGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAg                                                                                                              <  1:948244/150‑1 (MQ=255)
                                       aGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAg                                                                                                              <  2:1621791/150‑1 (MQ=255)
                                          aaTGATCGTCGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGGTCAATGCATATATTCCGAGAGCGGGTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTg                                                                                                           <  1:1278701/150‑1 (MQ=255)
                                          aaTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTg                                                                                                           <  1:1334178/150‑1 (MQ=255)
                                           aTGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGt                                                                                                          >  1:668595/1‑150 (MQ=255)
                                            tGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTa                                                                                                         <  2:668595/150‑1 (MQ=255)
                                             gATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGACAAGATGATGAACAGAAAAATCAGTCCAGCTGTac                                                                                                        >  1:411802/1‑150 (MQ=255)
                                                cGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATTCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACAcc                                                                                                     <  1:98052/150‑1 (MQ=255)
                                                  tAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCa                                                                                                   <  2:795933/150‑1 (MQ=255)
                                                    gggggtTAAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGCAAAATCAGTCACGCGGTTCACCCacc                                                                                                 >  2:738247/4‑148 (MQ=255)
                                                       ggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTTCCGTGCCAGCTGCCCGGCAATATGATTAACAGAAAAATCAGTCCCGCTGTACACCCAggcgg                                                                                              >  1:798499/1‑150 (MQ=255)
                                                       ggtTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGAACACCCAggcgg                                                                                              <  2:1441789/150‑1 (MQ=255)
                                                         ttCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCAAGGCAAGATGATGAACAGAAAAATCAGTCAAGCTGTACACCCAGgcggcg                                                                                            >  2:88362/1‑150 (MQ=255)
                                                           cAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcggg                                                                                          <  1:1290103/150‑1 (MQ=255)
                                                            agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTTCCGTGCCAGCTGCACGGCAAGATGATGAACACAAAAAACAGTCCAGCTGTACACCCAGGCggcgggc                                                                                         >  2:516127/1‑150 (MQ=255)
                                                            agagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcgggc                                                                                         <  2:236761/150‑1 (MQ=255)
                                                             gagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcgggcg                                                                                        >  2:1228967/1‑150 (MQ=255)
                                                             gagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCggcgggcg                                                                                        >  2:738129/1‑150 (MQ=255)
                                                             gagGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACCGCAAGATGATGAACAGAAAAATCAGTCCCGCTGGACACCCCGGCggcgggcg                                                                                        >  1:1433218/1‑150 (MQ=255)
                                                                gAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCACCTGCACCGCAAGATGATGATCAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACg                                                                                     >  2:498011/1‑150 (MQ=255)
                                                                 aaGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACgg                                                                                    <  1:986904/150‑1 (MQ=255)
                                                                    aTTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGGTCAATGCACATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCtt                                                                                 <  2:1518099/150‑1 (MQ=255)
                                                                        ccACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATa                                                                             >  1:212213/1‑150 (MQ=255)
                                                                           caGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGc                                                                          <  2:580428/150‑1 (MQ=255)
                                                                             ggAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGGACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCtt                                                                        >  1:1547017/1‑150 (MQ=255)
                                                                             ggAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCtt                                                                        >  1:988065/1‑150 (MQ=255)
                                                                               aaaGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGCTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCg                                                                      <  1:1347883/150‑1 (MQ=255)
                                                                                    gggATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAg                                                                 <  1:32499/150‑1 (MQ=255)
                                                                                      gATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGc                                                               <  2:897428/150‑1 (MQ=255)
                                                                                         gAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCAc                                                            >  2:207019/1‑150 (MQ=255)
                                                                                          aaaTGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCATCTGCAAGGCCAGATGATGAACAGAAAAATCAGTCAAGCTGTACACCCACGCGGCTGGCGACGGCTTCATAGGCGAAGATCAGTCCAcc                                                           >  1:326099/1‑150 (MQ=255)
                                                                                                 ggaggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCAAGCTACACTGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGctc                                                    <  2:268172/150‑1 (MQ=255)
                                                                                                    ggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTgg                                                 <  2:1367345/150‑1 (MQ=255)
                                                                                                    ggaATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTgg                                                 <  2:518397/150‑1 (MQ=255)
                                                                                                     caaacaGCGCTCAATGCATATATTCCGAGAGCGGCTGCCTTGCCGTGCCAGCGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTGCATAGGCTTCGATCAGGCCACCGAGGCTCTGGc                                                <  1:84569/145‑1 (MQ=255)
                                                                                                      aaTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCg                                               <  1:402631/150‑1 (MQ=255)
                                                                                                      aaTGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCg                                               <  1:396480/150‑1 (MQ=255)
                                                                                                            cGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCCCGGCAAGATGATGAACAGAAAAATCAGACCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGAACAGGCCACCCCGGCTCTGGCGGAAAcc                                         >  1:1626928/1‑149 (MQ=255)
                                                                                                                cAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAAACAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTGATAGGCTTAGATCAGGCCACCGACGCTCGGGCGGAAACGGTCt                                     >  1:704566/1‑150 (MQ=255)
                                                                                                                 aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGTCAAGATGATGAACAGATAAATCAGTCCAGCTGTACACCCTGGCGGCTGGCGACGGCTTCATAGGCGTAGATCAGGCCACCGACGCTCTGCCGGAATCGGTCtt                                    >  1:265461/1‑150 (MQ=255)
                                                                                                                 aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCtt                                    >  2:580999/1‑150 (MQ=255)
                                                                                                                 aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCtt                                    >  2:67223/1‑150 (MQ=255)
                                                                                                                 aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCtt                                    <  1:1032347/150‑1 (MQ=255)
                                                                                                                 aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCtt                                    <  1:1296709/150‑1 (MQ=255)
                                                                                                                 aaTGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCtt                                    <  2:514793/150‑1 (MQ=255)
                                                                                                                     cATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTc                                <  1:1261271/150‑1 (MQ=255)
                                                                                                                     cATATATTCCGAGAGCGGTTGTCATTCCGTGCCAGCAGCACGGCAAGATGATTAACACAAAAATCAGTACAGCTCTAAACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCCCCGAGGCACAGGCGGATACGGTCTTTGtt                                >  2:1400539/1‑149 (MQ=255)
                                                                                                                      atatatTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCTAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTcc                               <  1:959536/150‑1 (MQ=255)
                                                                                                                            tCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtc                         <  2:20374/150‑1 (MQ=255)
                                                                                                                             ccGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtct                        >  1:1110107/1‑150 (MQ=255)
                                                                                                                              cGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtctc                       <  1:1228967/150‑1 (MQ=255)
                                                                                                                              cGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTtctc                       <  1:738129/150‑1 (MQ=255)
                                                                                                                                  cgCGGGTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGc                   <  2:1433218/149‑1 (MQ=255)
                                                                                                                                   gCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCg                  <  2:798499/150‑1 (MQ=255)
                                                                                                                                     ggTTGTCTTGCCGTGCCAGCTGCTCGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTCCCCCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCGGGCGGAAACGGTCTTTGTCCATTTTCTGCAGCGtt                >  1:890548/1‑150 (MQ=255)
                                                                                                                                     ggTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAACCGGTCTTTGTCCATTTTCTCCCGCGtt                >  2:112414/1‑150 (MQ=255)
                                                                                                                                     ggTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCCTTTTCTCCAGCggt                >  1:131596/1‑148 (MQ=255)
                                                                                                                                     ggTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGtt                >  2:1023028/1‑150 (MQ=255)
                                                                                                                                        tGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCt             <  2:394205/150‑1 (MQ=255)
                                                                                                                                         gTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCGCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCtt            <  1:34159/150‑1 (MQ=255)
                                                                                                                                          tCTTGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCttt           >  1:1139056/1‑150 (MQ=255)
                                                                                                                                           cTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTg          >  1:1033022/1‑150 (MQ=255)
                                                                                                                                            ttGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGt         >  2:1322746/1‑150 (MQ=255)
                                                                                                                                            ttGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGt         <  1:1322746/150‑1 (MQ=255)
                                                                                                                                             tGCCGTGCAGCTGGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAAGCCCCCGCGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCCGCGTTTCTTTGTc        >  2:1564393/1‑150 (MQ=255)
                                                                                                                                              gCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTcc       <  1:983744/150‑1 (MQ=255)
                                                                                                                                              gCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTcc       >  2:449166/1‑150 (MQ=255)
                                                                                                                                              gCCGTGCCAGCTGCACGACAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTcc       <  2:411802/150‑1 (MQ=255)
                                                                                                                                               ccGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTccc      >  2:1564177/1‑150 (MQ=255)
                                                                                                                                               ccGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTccc      <  1:1430124/150‑1 (MQ=255)
                                                                                                                                                   gCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCACCGTTTCTTTGTCCCACAg  >  1:300745/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCATATATTCCGAGAGCGGTTGTCTTGCCGTGCCAGCTGCACGGCAAGATGATGAACAGAAAAATCAGTCCAGCTGTACACCCAGGCGGCGGGCGACGGCTTCATAGGCTTCGATCAGGCCACCGAGGCTCTGGCGGAAACGGTCTTTGTCCATTTTCTCCAGCGTTTCTTTGTCCCACAG  >  NC_000913/2596725‑2597021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: