Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,339,818 Δ1 bp 6.3% coding (248/810 nt) mlaF ← ABC transporter maintaining OM lipid asymmetry, ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,339,8180A. 6.3% 342.2 / 14.9 111coding (248/810 nt)mlaFABC transporter maintaining OM lipid asymmetry, ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (52/52);  new base . (2/5);  total (54/57)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

Only 100 of 112 total aligned reads displayed.

CGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCT  >  NC_000913/3339672‑3339967
                                                                                                                                                  |                                                                                                                                                      
cGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTg                                                                                                                                                     >  2:982934/1‑150 (MQ=255)
cGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTg                                                                                                                                                     <  2:1473453/150‑1 (MQ=255)
cGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGGATGTTCGCGCAGTGGAGAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTg                                                                                                                                                     <  2:942096/150‑1 (MQ=255)
cGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGGTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTg                                                                                                                                                     <  2:1420256/150‑1 (MQ=255)
 ggCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGt                                                                                                                                                    <  1:1338951/150‑1 (MQ=255)
 ggCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGt                                                                                                                                                    >  2:1134020/1‑150 (MQ=255)
    cTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGtata                                                                                                                                                 <  2:1094879/150‑1 (MQ=255)
        aGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAgg                                                                                                                                            <  1:1346534/150‑1 (MQ=255)
          cTTCATCATCACCGTACTAAGCAACAATGGCGCGGGAAGTTGGGTATGAGCGCGCAGTGGATAGGCGACGTTGTCAAAAACGGTCTTATCAGTGAACAACGCCGCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACGGgcg                                                                                                                                          >  2:1401809/1‑150 (MQ=255)
            tcatcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGAAAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGcgcg                                                                                                                                        >  1:853235/1‑150 (MQ=255)
               tcatcaCCGTACTATGCAACAATGGCGCGGGAAGTTTGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAAc                                                                                                                                     <  1:1514940/150‑1 (MQ=255)
                cttctgaactttccggtgggatggcgcggGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACg                                                                                                                                    <  1:997506‑M2/121‑1 (MQ=255)
                catcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACg                                                                                                                                    <  1:1481509/150‑1 (MQ=255)
                 atcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACga                                                                                                                                   <  1:1585736/150‑1 (MQ=255)
                 atcaCCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTCGCGCCTGTATACAGTGCGCAAACgg                                                                                                                                   <  2:446490/150‑2 (MQ=255)
                   caCCGTACTATGCAACAATGGCGCGGGAAGTTGAGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACgaga                                                                                                                                 >  2:1513524/1‑150 (MQ=255)
                     ccGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACa                                                                                                                               <  2:1351232/150‑1 (MQ=255)
                       gTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATc                                                                                                                             <  1:1134020/150‑1 (MQ=255)
                         aCTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGc                                                                                                                           >  1:183128/1‑150 (MQ=255)
                          ttccggtgggatggcgcggGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGcc                                                                                                                          <  1:617102‑M2/131‑1 (MQ=255)
                           tATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCg                                                                                                                         >  2:518782/1‑150 (MQ=255)
                            aTGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCgg                                                                                                                        >  1:781757/1‑150 (MQ=255)
                            aTGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCgg                                                                                                                        <  2:875540/150‑1 (MQ=255)
                             tGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGa                                                                                                                       >  1:507592/1‑150 (MQ=255)
                               caacaaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAat                                                                                                                     <  1:1415569/150‑1 (MQ=255)
                                  caaTGGCGCGGGAAGTTGGGTATGTTAGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCGCCGGACTGAAATAACATGCTCATCCGATTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATAtt                                                                                                                  >  1:837864/1‑150 (MQ=255)
                                   aaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATtc                                                                                                                 <  2:48059/150‑1 (MQ=255)
                                   aaTGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGAAGTTGTCAAATAAGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATtc                                                                                                                 <  1:354832/150‑1 (MQ=255)
                                    atggcgcggGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATTACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATtct                                                                                                                <  2:1146453‑M2/141‑1 (MQ=255)
                                      ggcgcggGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCa                                                                                                              <  2:877506‑M2/143‑1 (MQ=255)
                                       gcgcggGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCAc                                                                                                             <  1:1112136‑M2/144‑1 (MQ=255)
                                        cgcggGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCAcc                                                                                                            <  1:184297‑M2/145‑1 (MQ=255)
                                             gAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGa                                                                                                       <  2:1123408/150‑1 (MQ=255)
                                              aaGTTGGGTATGTTCGAGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCTGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaa                                                                                                      <  1:280716/150‑1 (MQ=255)
                                                gTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa                                                                                                    >  1:1357914/1‑150 (MQ=255)
                                                gTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGaaaa                                                                                                    <  1:897018/150‑1 (MQ=255)
                                                 gtGGGTATGTTCGCGCAGAGGAGAGGCGACGTTGTCAAATACGTTCAAATCAGTTAACAACGCCCCTGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAg                                                                                                   <  1:273164/149‑1 (MQ=255)
                                                   gtgTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGa                                                                                                 <  1:1658764/148‑1 (MQ=255)
                                                   gggTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGa                                                                                                 <  1:1665123/150‑1 (MQ=255)
                                                     gTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAtc                                                                                               <  2:594466/150‑1 (MQ=255)
                                                           tCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCa                                                                                         <  2:569982/150‑1 (MQ=255)
                                                            cgcgCAGTGGATAGGCGACGTTGTCTAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCCTGTATACAgtg                                                                                                                                            <  1:215911/98‑3 (MQ=255)
                                                            cgcgCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCAt                                                                                        >  1:970396/1‑150 (MQ=255)
                                                            cgcgCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCCTGTATACAgtg                                                                                                                                            >  2:215911/1‑96 (MQ=255)
                                                              cgcAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGa                                                                                      <  1:982934/150‑1 (MQ=255)
                                                               gcAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGAt                                                                                     <  1:996055/150‑1 (MQ=255)
                                                                cAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATc                                                                                    <  2:1357914/150‑1 (MQ=255)
                                                                cAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTATATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAtctc                                                                                             >  2:1477060/1‑141 (MQ=255)
                                                                cAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTATATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGAtctc                                                                                             <  1:1477060/141‑1 (MQ=255)
                                                                 aGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCt                                                                                   <  2:1673557/150‑1 (MQ=255)
                                                                 aGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCt                                                                                   <  2:1329950/150‑1 (MQ=255)
                                                                 aGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCt                                                                                   >  1:1485437/1‑150 (MQ=255)
                                                                    ggATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCCCCATCGAAAAGGATCTCACCATGATCTGGt                                                                                >  2:1564143/1‑150 (MQ=255)
                                                                      aTAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGc                                                                              <  1:1513524/150‑1 (MQ=255)
                                                                         ggCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAt                                                                           >  2:1532012/1‑150 (MQ=255)
                                                                          gCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGAtt                                                                          <  1:1683195/150‑1 (MQ=255)
                                                                              cgatGTCAAATACGTTCATATCAGTGAACAACGCACCGCACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGcc                                                                      >  2:501484/4‑150 (MQ=255)
                                                                              cGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGcc                                                                      >  2:645182/1‑150 (MQ=255)
                                                                               gTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGccc                                                                     >  1:367310/1‑150 (MQ=255)
                                                                                ttGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCg                                                                    >  1:730900/1‑150 (MQ=255)
                                                                                 tGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGc                                                                   >  2:802490/1‑150 (MQ=255)
                                                                                  gTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCATTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGcc                                                                  >  2:1330560/1‑150 (MQ=255)
                                                                                      aaTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATc                                                              >  2:583281/1‑150 (MQ=255)
                                                                                       aTACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCa                                                             >  1:627595/1‑150 (MQ=255)
                                                                                        tACGTTCATATCAGTGAACAGCGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAg                                                            >  2:550308/1‑150 (MQ=255)
                                                                                            ttCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCTAATCAGACgg                                                        >  1:450105/1‑150 (MQ=255)
                                                                                               atatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGt                                                     <  2:507592/150‑1 (MQ=255)
                                                                                                tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGCACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTa                                                    >  2:1541542/1‑150 (MQ=255)
                                                                                                tatCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTa                                                    >  1:895830/1‑150 (MQ=255)
                                                                                                   cAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACgg                                                        >  1:1603486/1‑143 (MQ=255)
                                                                                                   cAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACgg                                                        <  2:1603486/143‑1 (MQ=255)
                                                                                                       gAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGcgtcgt                                             >  1:1476253/1‑150 (MQ=255)
                                                                                                            gcGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTAc                                        >  1:629834/2‑150 (MQ=255)
                                                                                                             cGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTAcc                                       <  2:587008/150‑1 (MQ=255)
                                                                                                              gCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCg                                      >  1:1007063/1‑150 (MQ=255)
                                                                                                              gCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCg                                      >  2:1302297/1‑150 (MQ=255)
                                                                                                                   ggACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATGTTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGcc                                 <  1:758138/150‑1 (MQ=255)
                                                                                                                    gACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCTAATCAGACGGAGTAGCGTCGTTTTACCGATGccc                                <  2:450105/150‑1 (MQ=255)
                                                                                                                    gACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGccc                                >  2:44540/1‑150 (MQ=255)
                                                                                                                    gACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGccc                                <  1:1551609/150‑1 (MQ=255)
                                                                                                                     aCTGAANTAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCg                               <  2:1221539/150‑1 (MQ=255)
                                                                                                                        taaaTAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATg                            <  2:781757/149‑1 (MQ=255)
                                                                                                                        gAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATg                            >  2:482312/1‑150 (MQ=255)
                                                                                                                          aaTAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGc                          <  2:627595/150‑1 (MQ=255)
                                                                                                                              aCATGCTCATCCGTTTGCGCACGGTATACAGGCGCGAACGAGACATCGCCGGTATATTCTCACCATCGAAAAGGATCTCGCCATGATCTGGTGCGATTTGACCGCCAATCAGACGGTGTAGCGTCGTTTTACCGATGCCCGAAGGCCCCa                      >  1:252880/1‑150 (MQ=255)
                                                                                                                                aTGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATg                    <  2:1661435/150‑1 (MQ=255)
                                                                                                                                 tGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGa                   >  2:1584617/1‑150 (MQ=255)
                                                                                                                                  gCTCATCCATTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGAt                  <  1:1330560/150‑1 (MQ=255)
                                                                                                                                   cTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATc                 >  1:396800/1‑150 (MQ=255)
                                                                                                                                    acATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATGGAAAAGGATCTCACCATGATCTGGTGCGTTTTTCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCg                <  2:1630946/149‑1 (MQ=255)
                                                                                                                                     cATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGc               >  1:565081/1‑150 (MQ=255)
                                                                                                                                     cATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGc               >  1:173214/1‑150 (MQ=255)
                                                                                                                                      aTCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGcc              >  2:688887/1‑150 (MQ=255)
                                                                                                                                         cGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg           <  2:183128/150‑1 (MQ=255)
                                                                                                                                         cGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg           >  2:87263/1‑150 (MQ=255)
                                                                                                                                         cGTTCGCGCCTGTATACAGTGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTg           >  2:482019/1‑150 (MQ=255)
                                                                                                                                              gcgcACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCtt      >  1:967962/1‑150 (MQ=255)
                                                                                                                                               cgcACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGTTCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGACCCATGATCGCCGTGATCTTc     >  2:891841/1‑150 (MQ=255)
                                                                                                                                                 cACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTccc   <  1:1685027/150‑1 (MQ=255)
                                                                                                                                                  aCTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCt  <  2:1425504/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
CGGCCTCCAGCTTCATCATCACCGTACTATGCAACAATGGCGCGGGAAGTTGGGTATGTTCGCGCAGTGGATAGGCGACGTTGTCAAATACGTTCATATCAGTGAACAACGCCCCGGACTGAAATAACATGCTCATCCGTTTGCGCACTGTATACAGGCGCGAACGAGACATCGCCGGAATATTCTCACCATCGAAAAGGATCTCACCATGATCTGGTGCGATTTGCCCGCCAATCAGACGGAGTAGCGTCGTTTTACCGATGCCCGATGGCCCCATGATCGCCGTGATCTTCCCT  >  NC_000913/3339672‑3339967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: