Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,062,181 Δ1 bp 7.1% intergenic (+36/‑66) yihM → / → yihN putative sugar phosphate isomerase/MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,062,1810C. 7.1% 486.5 / 29.8 155intergenic (+36/‑66)yihM/yihNputative sugar phosphate isomerase/MFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base C (78/65);  new base . (8/3);  total (87/68)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 161 total aligned reads displayed.

TTACTGGATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGAT  >  NC_000913/4062032‑4062330
                                                                                                                                                     |                                                                                                                                                       
 tACTGGATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGAAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTcc                                                                                                                                                        >  1:33825/1‑150 (MQ=255)
   cTGGATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCat                                                                                                                                                      >  1:1624475/1‑150 (MQ=255)
   cTGGATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCat                                                                                                                                                      >  1:743202/1‑150 (MQ=255)
    tGGATGCTCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCata                                                                                                                                                     <  1:393847/150‑1 (MQ=255)
     ggATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCatat                                                                                                                                                    >  2:1337845/1‑150 (MQ=255)
      gATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATg                                                                                                                                                  >  1:61188/1‑150 (MQ=255)
          cgcgGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCATATggaatg                                                                                                                                             >  2:751419/1‑145 (MQ=255)
            cgGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGt                                                                                                                                           >  1:132988/1‑150 (MQ=255)
              gTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCg                                                                                                                                         <  1:622420/150‑1 (MQ=255)
              gTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCg                                                                                                                                         >  2:1642153/1‑150 (MQ=255)
              gTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCg                                                                                                                                         >  1:553206/1‑150 (MQ=255)
               ttACGTAAAGAAAAAGAAGATGCAATTTATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAGACAAGAGGCAACCCATCTTCTGAACCACTAATAAGTTTTAACTCAGCCGCAGGTAATAAAACCATTCCATTTGAATGGTCgg                                                                                                                                        >  2:148142/1‑150 (MQ=255)
               ttACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTATAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCgg                                                                                                                                        >  2:1582482/1‑150 (MQ=255)
                tACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCg                                                                                                                                         >  1:1714619/1‑148 (MQ=255)
                tACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCg                                                                                                                                         <  2:1714619/148‑1 (MQ=255)
                  cGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTAt                                                                                                                                     >  1:1380406/1‑150 (MQ=255)
                  cGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTAt                                                                                                                                     >  2:1162189/1‑150 (MQ=255)
                  cGTAAAGAAAAAGAAGAGGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTACATATGAATGGTCGGTAt                                                                                                                                     >  1:1633807/1‑150 (MQ=255)
                   gTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATc                                                                                                                                    >  1:1423999/1‑150 (MQ=255)
                      aagaaaaagaaGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTa                                                                                                                                 <  1:452746/150‑1 (MQ=255)
                         aaaaagaaGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTg                                                                                                                              >  2:492004/1‑150 (MQ=255)
                           aaagaaGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGcc                                                                                                                            <  2:494934/150‑1 (MQ=255)
                             agaagaTTCAATTAATCAGATAAATCATGTGCGTAAGTACTACAACAAATCAAAAAAGACGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATCTGAATGGTCGGTATCTTATTGCCttt                                                                                                                         <  1:253695/150‑1 (MQ=255)
                               aatattcAATTAAACAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTa                                                                                                                        <  1:1520567/144‑1 (MQ=255)
                               aagaTGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTa                                                                                                                        <  2:1363014/150‑1 (MQ=255)
                               aagaTGCAATTAATCAGATAAATCATGTGCGTAACGTACTAAAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTa                                                                                                                        <  2:163059/150‑1 (MQ=255)
                                   tGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAAc                                                                                                                    >  1:1581054/1‑150 (MQ=255)
                                     cAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAg                                                                                                                  <  1:1606289/150‑1 (MQ=255)
                                     cAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAg                                                                                                                  >  1:1156982/1‑150 (MQ=255)
                                     cAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAg                                                                                                                  >  1:1073265/1‑150 (MQ=255)
                                         tAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGAt                                                                                                              >  2:939615/1‑150 (MQ=255)
                                           aTCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGAttt                                                                                                            >  2:1104296/1‑150 (MQ=255)
                                           aTCAGATAAATCATGTGCGTAAAGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGAttt                                                                                                            <  1:1649739/150‑1 (MQ=255)
                                             cAGATAAATCATGTGCGTAACGTACTTCAACAAATCAAACAAGAGGCAAACCATCTACTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTa                                                                                                          >  2:1010172/1‑150 (MQ=255)
                                             cAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTAGATTATTGCCTTTAAAACAGTGATTTTa                                                                                                          >  1:1197498/1‑150 (MQ=255)
                                               gATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTaaa                                                                                                        <  2:743014/150‑1 (MQ=255)
                                                   aaTCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATc                                                                                                    <  2:1306821/150‑1 (MQ=255)
                                                         gtgCGTAACGTACTACAACAATCAAACAAGAGGCTAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAgg                                                                                             <  1:1311601/150‑1 (MQ=255)
                                                           gCGTAACTTAATACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAATTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTTTTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAggg                                                                                            <  2:66555/150‑1 (MQ=255)
                                                             ttAACGTACTACAAAAAATCAAACAATAGGCAAACAATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGa                                                                                          <  1:1470883/149‑1 (MQ=255)
                                                             gTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGa                                                                                          >  2:1003624/1‑150 (MQ=255)
                                                                aCGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGt                                                                                       <  2:713826/150‑1 (MQ=255)
                                                                 cGTACTACAACAACTCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTGATAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGGCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGtt                                                                                      <  2:1063983/150‑1 (MQ=255)
                                                                  gTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTa                                                                                     <  1:650091/150‑1 (MQ=255)
                                                                  gTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGGCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTa                                                                                     <  1:1052509/150‑1 (MQ=255)
                                                                   tactacAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTAt                                                                                    <  1:1168057/150‑1 (MQ=255)
                                                                   tactacAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTAt                                                                                    >  1:1241004/1‑150 (MQ=255)
                                                                    actacAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATg                                                                                   <  1:411248/150‑1 (MQ=255)
                                                                     ctacAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCAAACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGc                                                                                  >  2:785917/1‑150 (MQ=255)
                                                                       acaacaAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTc                                                                                >  1:527579/1‑150 (MQ=255)
                                                                        caacaaATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCa                                                                               >  2:1424874/1‑150 (MQ=255)
                                                                        aaacaaATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCa                                                                               <  1:265806/149‑1 (MQ=255)
                                                                         aacaaATCAAACAAGAGGCAAACCATCTTCTGCTCCACTAATAAGTTTTAACTCATACGCAGTTAAAAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCAc                                                                              >  1:259558/1‑150 (MQ=255)
                                                                         aacaaATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCAc                                                                              <  2:61188/150‑1 (MQ=255)
                                                                          acaaATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACg                                                                             >  1:806417/1‑150 (MQ=255)
                                                                          acaaATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACg                                                                             <  1:945020/150‑1 (MQ=255)
                                                                                 aaaCAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATGGCTTAGGGGATGTTATGCTCACGAAAAAGa                                                                      >  1:874146/1‑150 (MQ=255)
                                                                                      agagGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATgg                                                                 >  2:30884/1‑150 (MQ=255)
                                                                                      agagGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATgg                                                                 <  1:492004/150‑1 (MQ=255)
                                                                                        agGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGc                                                               >  1:359348/1‑150 (MQ=255)
                                                                                         ggCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCg                                                              >  1:1596558/1‑150 (MQ=255)
                                                                                               ccATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTAttt                                                        >  2:342038/1‑150 (MQ=255)
                                                                                                cATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGATTAGGGGATGTTATGCTCACGAAAAAGAAATGGGGTTTAtttt                                                       >  2:341466/1‑149 (MQ=255)
                                                                                                 aTCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAg                                                      >  2:1116996/1‑150 (MQ=255)
                                                                                                 aTCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAg                                                      >  2:1207974/1‑150 (MQ=255)
                                                                                                    ttctGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCt                                                   <  1:1249153/150‑1 (MQ=255)
                                                                                                    ttctGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCt                                                   <  2:226574/150‑1 (MQ=255)
                                                                                                       tGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTAtt                                                >  1:1353118/1‑150 (MQ=255)
                                                                                                       tGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTAtt                                                >  2:1576012/1‑150 (MQ=255)
                                                                                                        gAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTa                                               >  2:1444877/1‑150 (MQ=255)
                                                                                                          aCCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAac                                             <  2:16109/150‑1 (MQ=255)
                                                                                                             aCTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTaa                                              <  1:1181132/146‑1 (MQ=255)
                                                                                                             aCTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTaa                                              >  2:1181132/1‑146 (MQ=255)
                                                                                                              cTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTTAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACtg                                         <  2:1246972/150‑1 (MQ=255)
                                                                                                                     gTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCg                                  >  1:129007/1‑150 (MQ=255)
                                                                                                                     gTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCg                                  >  1:1404520/1‑150 (MQ=255)
                                                                                                                                 aCGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTAAGGGGATGTTATGCTCAAGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTtata                      >  1:450145/1‑150 (MQ=255)
                                                                                                                                  cGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATaa                     >  2:876838/1‑150 (MQ=255)
                                                                                                                                  cGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATaa                     <  1:912247/150‑1 (MQ=255)
                                                                                                                                        taataaAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTAcc               >  2:40753/1‑150 (MQ=255)
                                                                                                                                        taataaAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGATCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTAcc               >  1:822627/1‑150 (MQ=255)
                                                                                                                                          taccgaCCATTCATATGGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATaaa                    >  1:1545176/6‑143 (MQ=255)
                                                                                                                                          taccgaCCATTCATATGTAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATaaa                    <  2:1545176/138‑1 (MQ=255)
                                                                                                                                          ataaAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGt             <  1:939615/150‑1 (MQ=255)
                                                                                                                                           taaAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTtata                      >  1:200804/1‑140 (MQ=255)
                                                                                                                                           accgaCCATTCATATGGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTc            >  1:324821/5‑150 (MQ=255)
                                                                                                                                            ccgaCCATTCATATGGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCg           >  2:842847/4‑150 (MQ=255)
                                                                                                                                            ccgaCCATTCATATGGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCg           >  1:666089/4‑150 (MQ=255)
                                                                                                                                            ccgaCCATTCATATGGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCg           >  2:205860/4‑150 (MQ=255)
                                                                                                                                            ccgaCCATTCATATGGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCg           >  1:915356/4‑150 (MQ=255)
                                                                                                                                              aaCCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTGTAAATTACCGTCGCt         <  2:583329/150‑1 (MQ=255)
                                                                                                                                              aaCCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCt         <  1:1650327/150‑1 (MQ=255)
                                                                                                                                               aCCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTg        >  1:1655975/1‑150 (MQ=255)
                                                                                                                                                ccATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGa       <  2:1391866/150‑1 (MQ=255)
                                                                                                                                                ccATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGa       <  2:889838/150‑1 (MQ=255)
                                                                                                                                                 cATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGaa      >  1:1128279/1‑150 (MQ=255)
                                                                                                                                                 cATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGaa      <  1:1634783/150‑1 (MQ=255)
                                                                                                                                                   ttCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAg    >  2:1672230/1‑150 (MQ=255)
                                                                                                                                                   ttCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAg    >  2:1425932/1‑150 (MQ=255)
                                                                                                                                                    tCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGa   >  2:1281119/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TTACTGGATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGAT  >  NC_000913/4062032‑4062330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: