Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,449,624 Δ1 bp 6.2% coding (426/822 nt) yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,6240C. 6.2% 362.0 / 14.6 113coding (426/822 nt)yfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (59/47);  new base . (2/5);  total (61/52)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 117 total aligned reads displayed.

TACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTTTGTT  >  NC_000913/2449475‑2449773
                                                                                                                                                      |                                                                                                                                                     
 aCGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACg                                                                                                                                                      >  1:355276/1‑150 (MQ=255)
   gTTGAAAGCGTTTTCTCAACCACACCCCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTc                                                                                                                                                    <  1:627452/150‑1 (MQ=255)
    ttGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCa                                                                                                                                                   <  1:1496385/150‑1 (MQ=255)
    ttGAAAGCGTTATCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGCATGGACAAATCGTGTCGTGACTATCAGGGGCCTGATAGTCAc                                                                                                                                                  <  2:333949/150‑1 (MQ=255)
     tGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGAGATAATTCTCAATCCATGAAACGTCATAAGGTTGTACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCAc                                                                                                                                                  <  1:405203/150‑1 (MQ=255)
     tGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGGGCCTGATAGTCACg                                                                                                                                                 <  2:182970/150‑1 (MQ=255)
          gCGTTTTCTCAAACACACCGCCCTTAGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                                                                                             <  2:60811/150‑5 (MQ=255)
                tctcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgag                                                                                                                                       <  2:1113982/150‑1 (MQ=255)
                 ctcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                                                                                      >  1:1103960/1‑150 (MQ=255)
                  tcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                                                                                     <  1:1702447/150‑1 (MQ=255)
                  tcAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAAGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                                                                                     >  1:630743/1‑150 (MQ=255)
                     aCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCt                                                                                                                                  <  2:926162/150‑1 (MQ=255)
                     aCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCt                                                                                                                                  >  2:771039/1‑150 (MQ=255)
                     aCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCt                                                                                                                                  >  1:1104220/1‑150 (MQ=255)
                     aCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCt                                                                                                                                  >  1:1110022/1‑150 (MQ=255)
                     aCCACACCGCCCGTCGACTGCAGGGGGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCt                                                                                                                                  <  2:1087909/150‑1 (MQ=255)
                      ccACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAAGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                                                                                                 <  2:630743/150‑1 (MQ=255)
                        acacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCa                                                                                                                               >  2:364300/1‑150 (MQ=255)
                        acacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTACATGAAACGTCATAAGTTTGAACAGAAACGGAGGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCa                                                                                                                               <  1:1122168/150‑1 (MQ=255)
                         cacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCag                                                                                                                              <  2:531920/150‑1 (MQ=255)
                           ccGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                                                                            >  2:578170/1‑150 (MQ=255)
                           ccGCCCGTCGACTGCAGGGGGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                                                                            <  1:818701/150‑1 (MQ=255)
                            cGCCCGTCGCCTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                                                                                           >  1:344683/1‑150 (MQ=255)
                            cGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                                                                                           <  2:1637280/150‑1 (MQ=255)
                            cGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                                                                                           >  1:1558809/1‑150 (MQ=255)
                                cGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcatt                                                                                                                       >  2:1315333/1‑150 (MQ=255)
                                cGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcatt                                                                                                                       <  1:1377210/150‑1 (MQ=255)
                                   cGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattcat                                                                                                                    >  2:1419048/1‑150 (MQ=255)
                                   cGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattcat                                                                                                                    >  1:191633/1‑150 (MQ=255)
                                     aCTGCAGTGTTAGCAGTTTTTACTTACTGACATAATTCTCTTTCCATGAAACGTCATAAGGTTGAACAGTAACGGATGGACAAATCGTGTCGTGACTATCATGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataa                                                                                                                  <  1:312099/150‑1 (MQ=255)
                                      cTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataat                                                                                                                 >  1:148584/1‑150 (MQ=255)
                                           gggTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacca                                                                                                            >  1:511380/1‑150 (MQ=255)
                                            ggTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCTTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTAAAGAGTCATTCATAATaacaaa                                                                                                           >  1:816581/1‑150 (MQ=255)
                                                aGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGGTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAt                                                                                                       <  1:1371609/150‑1 (MQ=255)
                                                 gCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATg                                                                                                      >  2:1682517/1‑150 (MQ=255)
                                                  ctgTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGGCACGCCCCCTGGAGAGGCTTCAGAGGCATTCATAATAACCAACCATgg                                                                                                     <  2:1702068/148‑1 (MQ=255)
                                                   aGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGa                                                                                                    <  1:1205388/150‑1 (MQ=255)
                                                    gTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                                                                                                   >  1:207391/1‑150 (MQ=255)
                                                                 gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCTTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                                                                                      >  2:1466483/1‑150 (MQ=255)
                                                                 gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                                                                                      >  2:1473155/1‑150 (MQ=255)
                                                                 gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                                                                                      >  1:1307022/1‑150 (MQ=255)
                                                                  aCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAt                                                                                     >  2:770790/1‑150 (MQ=255)
                                                                   cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTATGAtt                                                                                    >  1:1075366/1‑150 (MQ=255)
                                                                   cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAtt                                                                                    >  1:445284/1‑150 (MQ=255)
                                                                    aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTc                                                                                   <  2:1103960/150‑1 (MQ=255)
                                                                     tAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa                                                                                  <  1:545450/150‑1 (MQ=255)
                                                                      aaTTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGCTGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCaa                                                                                 <  2:323019/150‑1 (MQ=255)
                                                                       aTTCTCATTACATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAt                                                                                <  1:599530/150‑1 (MQ=255)
                                                                        ttCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc                                                                               <  1:357667/150‑1 (MQ=255)
                                                                            cATTCCATGAAACGTCATAATGTTGAACAGAACCGGATGGACACATCGTGTCGTGACTATAAGGCCCATGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTg                                                                           <  1:873036/150‑1 (MQ=255)
                                                                            cATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGACCCCTGGAGAGGCTTCAGAGTCATTCTTAATAACCATCCATGGTCAAGAAAAGTAAGATTCAATCTCTg                                                                           >  1:111179/1‑150 (MQ=255)
                                                                             aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc                                                                          >  2:1690235/1‑150 (MQ=255)
                                                                             aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCGTCAGAGTCATTCATAATAACCAACCATGCGCAAGAAAAGTTAGATTCCATCTCTGc                                                                          >  1:281602/1‑150 (MQ=255)
                                                                              ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa                                                                         <  2:445284/150‑1 (MQ=255)
                                                                              ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGCCTTCAGAGTCAATCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa                                                                         >  1:930068/1‑150 (MQ=255)
                                                                                 cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag                                                                      >  1:1449135/1‑150 (MQ=255)
                                                                                   tGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc                                                                    <  2:1307022/150‑1 (MQ=255)
                                                                                    gAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACa                                                                   <  1:1315333/150‑1 (MQ=255)
                                                                                      aaCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAAt                                                                 >  2:1419950/1‑150 (MQ=255)
                                                                                           cATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCt                                                            >  2:1107870/1‑150 (MQ=255)
                                                                                                ggTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAAt                                                       <  2:207391/150‑1 (MQ=255)
                                                                                                ggTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGGCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACTTCAAAt                                                       >  2:1337467/1‑150 (MQ=255)
                                                                                                 gTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATg                                                      >  1:1695899/1‑150 (MQ=255)
                                                                                                 gTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATg                                                      >  1:234045/1‑150 (MQ=255)
                                                                                                   tGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATACCCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCATAGACAATAACCGCAAAAGta                                                    >  2:275937/1‑150 (MQ=255)
                                                                                                   tGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGta                                                    <  1:69723/150‑1 (MQ=255)
                                                                                                   tGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGta                                                    >  2:159606/1‑150 (MQ=255)
                                                                                                    taaCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtat                                                   <  1:1682517/149‑1 (MQ=255)
                                                                                                        aGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTataata                                               >  1:1347693/1‑150 (MQ=255)
                                                                                                           aaCGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATAccc                                            >  2:1308557/1‑150 (MQ=255)
                                                                                                             cGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGc                                          >  2:840041/1‑150 (MQ=255)
                                                                                                               gATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAAGCAACCATGGACAAGAAAAGTAAGCTTCAAGCTCTGCAGAGACAATCACCTCATATGTATAATACACGCAg                                        >  2:296605/1‑150 (MQ=255)
                                                                                                                  ggACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGTAGAGACAATAACCTCAAATGTATAATACCCGCAGTTc                                     >  1:337526/1‑150 (MQ=255)
                                                                                                                  ggACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTc                                     <  1:159606/150‑1 (MQ=255)
                                                                                                                  ggACAAATCGTGTCGTGACTATCAGGACCCTGATACGTCACTCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTc                                     <  1:275937/150‑1 (MQ=255)
                                                                                                                   gACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATATCCAACCATGGACAAGAAAAGTAAGATTGAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCAGCAGTAcc                                    >  1:264382/1‑150 (MQ=255)
                                                                                                                    aCAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCa                                   <  2:1558809/150‑1 (MQ=255)
                                                                                                                             gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACgg                          >  1:957732/1‑150 (MQ=255)
                                                                                                                             gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACgg                          >  1:1327492/1‑150 (MQ=255)
                                                                                                                             ggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACgg                          >  1:753957/3‑150 (MQ=255)
                                                                                                                              tcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGt                         <  2:148584/150‑1 (MQ=255)
                                                                                                                                gtgacgtATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTTAGATTCAATCTCTGCAGAGTCATTATGCTCAAATGTATTATACCCGCAGTTCCACCCAGACGGTag                       >  2:289344/7‑149 (MQ=255)
                                                                                                                                  gACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATc                     <  2:620352/150‑1 (MQ=255)
                                                                                                                                      tATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATATCCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAg                  <  2:1467846/150‑1 (MQ=255)
                                                                                                                                      tATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAAGCATGGACAACAAAAGGAAGATTCAATCTCTGCAGAGAAAATAACCTCAATTGTAAAATACCCGAAGTACCACACAGCAGGTAATCCAg                  >  2:83106/1‑150 (MQ=255)
                                                                                                                                       aTCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTGAGAGTCATTCATAATAACCAACCATGTACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTTAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCTgg                 >  2:292186/1‑150 (MQ=255)
                                                                                                                                       aTCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAgg                 <  1:423805/150‑1 (MQ=255)
                                                                                                                                       aTCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAgg                 >  1:1616727/1‑150 (MQ=255)
                                                                                                                                           ggCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACaa             <  2:344683/150‑1 (MQ=255)
                                                                                                                                           ggCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACaa             >  2:619854/1‑150 (MQ=255)
                                                                                                                                            gCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGCGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAAc            >  1:546099/1‑150 (MQ=255)
                                                                                                                                              cccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACtt          >  2:621955/1‑150 (MQ=255)
                                                                                                                                              cccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACtt          >  1:1302981/1‑150 (MQ=255)
                                                                                                                                               ccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTTCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTg         <  1:23109/150‑1 (MQ=255)
                                                                                                                                                 tGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCt       >  2:1100817/1‑150 (MQ=255)
                                                                                                                                                  taTACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCtt      <  2:1475084/149‑1 (MQ=255)
                                                                                                                                                   aTACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCttt     <  1:840041/150‑1 (MQ=255)
                                                                                                                                                      cGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATACCCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTttgtt  >  1:703768/1‑150 (MQ=255)
                                                                                                                                                      cGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTttgtt  <  2:355276/150‑1 (MQ=255)
                                                                                                                                                      cGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTttgtt  <  1:716619/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TACGTTGAAAGCGTTTTCTCAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTGCTTTGTT  >  NC_000913/2449475‑2449773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: