Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,729,399:1 +T 8.4% intergenic (‑28/+44) xylB ← / ← xylA xylulokinase/D‑xylose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,729,3991.T 8.4% 380.6 / 33.0 107intergenic (‑28/+44)xylB/xylAxylulokinase/D‑xylose isomerase
Reads supporting (aligned to +/- strand):  ref base . (49/49);  new base T (7/2);  total (56/51)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 107 total aligned reads displayed.

AGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAA  >  NC_000913/3729254‑3729544
                                                                                                                                                  |                                                                                                                                                 
agagTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGTTATc                                                                                                                                               >  2:1009566/1‑150 (MQ=255)
 tagTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATATCTTTTACGCCCGAGGTGCCAAGATCTCTCCCGATATACATATCGATCGTTCCTTAAAAAAATTCCCTGTATCg                                                                                                                                              <  1:1278869/149‑1 (MQ=255)
   gTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCCGCACCCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTGCTTAAAAAAATGCCCGGTATCGCt                                                                                                                                            >  2:829059/1‑150 (MQ=255)
   gTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCt                                                                                                                                            >  2:668358/1‑150 (MQ=255)
      gATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTAcc                                                                                                                                         >  2:1653587/1‑150 (MQ=255)
       aTGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCg                                                                                                                                        >  1:747451/1‑150 (MQ=255)
       aTGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCg                                                                                                                                        <  2:433608/150‑1 (MQ=255)
          cGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATa                                                                                                                                     <  2:1244809/150‑1 (MQ=255)
           gtgcgcGAAACGGTCAGCTTTTCCGTTTGCGAAGAAACCACCTCACACTGATCGTTGATCAAAATAACTTTTACGCCCGAGGTTCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATaa                                                                                                                                    <  1:1191131/148‑1 (MQ=255)
            ggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAAc                                                                                                                                   <  2:1173828/150‑1 (MQ=255)
                cgAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCggg                                                                                                                               <  1:1614920/150‑1 (MQ=255)
                cgAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCggg                                                                                                                               <  1:792099/150‑1 (MQ=255)
                cgAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCggg                                                                                                                               >  1:614936/1‑150 (MQ=255)
                cgAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCggg                                                                                                                               >  2:1596036/1‑150 (MQ=255)
                cgAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCggg                                                                                                                               >  1:1609830/1‑150 (MQ=255)
                 gAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGc                                                                                                                              <  1:1653587/150‑1 (MQ=255)
                  aaaCGGTCATCTTTTGCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGcc                                                                                                                             <  2:337905/150‑1 (MQ=255)
                  aaaCGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGcc                                                                                                                             >  2:529232/1‑150 (MQ=255)
                        tCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGa                                                                                                                       <  1:1345036/150‑1 (MQ=255)
                          aGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACt                                                                                                                     <  1:1191815/150‑1 (MQ=255)
                               ttCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGcaca                                                                                                                <  1:22486/150‑1 (MQ=255)
                                 ccGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGACAAGCTATATCCCGATATTCATATCGATCGTACCTTTAAAAAATGCCCGGTATCGCTACCGATACCCGGGCCAGCGGGCTGCCCAGt                                                                                                              >  2:1169106/1‑150 (MQ=255)
                                  cGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGtt                                                                                                             <  2:1554628/150‑1 (MQ=255)
                                  cGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGtt                                                                                                             <  2:1488440/150‑1 (MQ=255)
                                  cGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGtt                                                                                                             <  2:1634230/150‑1 (MQ=255)
                                     ttGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGc                                                                                                          <  2:1586296/150‑1 (MQ=255)
                                     ttGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAACATCTATCCCGATATACATATCGATCGGTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGc                                                                                                          <  1:319748/150‑1 (MQ=255)
                                      tGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGcc                                                                                                         >  1:457560/1‑150 (MQ=255)
                                       gcgcAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATTTACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCg                                                                                                        <  1:432297/150‑1 (MQ=255)
                                       gcgcAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCg                                                                                                        <  2:723300/150‑1 (MQ=255)
                                        cgcAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGt                                                                                                       >  1:396649/1‑150 (MQ=255)
                                        cgcAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGt                                                                                                       >  2:823286/1‑150 (MQ=255)
                                            gcaACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAtt                                                                                                   >  1:1340682/1‑150 (MQ=255)
                                                  accTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGa                                                                                             <  1:384571/150‑1 (MQ=255)
                                                        cccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAt                                                                                                    <  2:1636582/137‑1 (MQ=255)
                                                        cccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGAt                                                                                       <  2:396649/150‑1 (MQ=255)
                                                        cccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCAAACGGACTGCACAGTTAGCCGTTAt                                                                                                    >  1:1636582/1‑137 (MQ=255)
                                                         ccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATa                                                                                      >  1:1102947/1‑150 (MQ=255)
                                                             cTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATgg                                                                                  >  1:860955/1‑150 (MQ=255)
                                                              tCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGt                                                                                 >  2:593009/1‑150 (MQ=255)
                                                               cGTTGAGCAAAATAACTTTTTCGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGtt                                                                                >  1:657275/1‑150 (MQ=255)
                                                               cGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGtt                                                                                >  1:1427680/1‑150 (MQ=255)
                                                                  tGAGCAAAATAACTTTCACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTaa                                                                             >  2:1425320/1‑149 (MQ=255)
                                                                      cAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGa                                                                         >  2:633190/1‑150 (MQ=255)
                                                                      cAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGa                                                                         >  1:1015491/1‑150 (MQ=255)
                                                                      cAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGa                                                                         >  1:1268460/1‑150 (MQ=255)
                                                                        aaaTAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGAAAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGAtt                                                                       <  2:686556/150‑1 (MQ=255)
                                                                          aTAACTTTTACGCCTGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCATAtttt                                                                     <  1:664168/150‑1 (MQ=255)
                                                                           tAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATACCCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTc                                                                    >  1:1318734/1‑150 (MQ=255)
                                                                             aCTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCa                                                                  >  2:1173521/1‑150 (MQ=255)
                                                                              cTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAg                                                                 >  1:1155871/1‑150 (MQ=255)
                                                                              cTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAg                                                                 <  1:465333/150‑1 (MQ=255)
                                                                                    cGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTc                                                           >  1:1595044/1‑150 (MQ=255)
                                                                                       ccGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCATTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTgg                                                       <  2:1207157/150‑1 (MQ=255)
                                                                                       ccGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTAAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTg                                                        >  1:404648/1‑150 (MQ=255)
                                                                                        cGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTgg                                                       <  2:725193/150‑1 (MQ=255)
                                                                                          agttGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCg                                                     <  1:493040/147‑1 (MQ=255)
                                                                                             tGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGAcc                                                  <  1:823286/150‑1 (MQ=255)
                                                                                                cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGCTTTTCCAGTTGTTCCTGGCGACCAct                                               >  2:236288/1‑150 (MQ=255)
                                                                                                  aGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCActct                                             <  2:1015219/150‑1 (MQ=255)
                                                                                                   gATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCActctt                                            >  1:1335786/1‑149 (MQ=255)
                                                                                                     tCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGAt                                          <  1:668358/150‑1 (MQ=255)
                                                                                                      cTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATg                                         <  2:769312/150‑1 (MQ=255)
                                                                                                       tATCCCGATATACATATCGATCGTTCCTTAAAAAATTGCCGGGTTTCGCTACCGATATCCGGGCCATCGGACTGCACAGTTAGCCGTTATTTGTCGATCAGATATTGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGc                                        <  1:924175/150‑1 (MQ=255)
                                                                                                       tATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGc                                        <  2:1015491/150‑1 (MQ=255)
                                                                                                       tATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGc                                        >  1:1460192/1‑150 (MQ=255)
                                                                                                          cccGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCAcc                                     >  1:1480831/1‑150 (MQ=255)
                                                                                                          cccGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTAGCGACCACTCTGATGCAcc                                     <  2:418538/150‑1 (MQ=255)
                                                                                                                ataCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGAc                               <  2:614936/150‑1 (MQ=255)
                                                                                                                ataCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGAc                               <  2:1609830/150‑1 (MQ=255)
                                                                                                                ataCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAATTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTCGTTCCTGGCGACCACTCTGATGCACCGGAGAc                               <  2:747451/150‑1 (MQ=255)
                                                                                                                 taCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACa                              >  1:51236/1‑150 (MQ=255)
                                                                                                                 taCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACa                              <  2:1102947/150‑1 (MQ=255)
                                                                                                                    atatCGATAGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTTCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAat                           <  1:350837/150‑1 (MQ=255)
                                                                                                                       tcgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTCCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGATAAatgat                        >  1:743101/1‑150 (MQ=255)
                                                                                                                       tcgatcgTTCCTTAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTTCCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGTCAAatgatg                       >  2:1301821/1‑150 (MQ=255)
                                                                                                                        cgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatgatg                       <  2:415386/150‑1 (MQ=255)
                                                                                                                         gatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGt                      >  1:48317/1‑150 (MQ=255)
                                                                                                                         gatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAAACGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTACAGTTGTTCCTCTCGACCACTCAGATGCACCTGAGACAAATGATGt                      >  1:1664718/1‑150 (MQ=255)
                                                                                                                          atcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGtt                     <  2:1441787/150‑1 (MQ=255)
                                                                                                                              ttCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTg                 <  2:451920/150‑1 (MQ=255)
                                                                                                                              ttCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTg                 <  2:458252/150‑1 (MQ=255)
                                                                                                                                ccTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAg               <  2:457560/150‑1 (MQ=255)
                                                                                                                                ccTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAg               >  2:710952/1‑150 (MQ=255)
                                                                                                                                ccTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAg               <  2:1480831/150‑1 (MQ=255)
                                                                                                                                  ttAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCa             >  1:1122238/1‑150 (MQ=255)
                                                                                                                                   tAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCat            >  1:1606004/1‑150 (MQ=255)
                                                                                                                                   tAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATCGTATACCAGATTTTCCAGTTGTTCCTGGCGACCACTATGAATCACAGGAGACAAATGATGTTCCTGAGCat            >  2:267862/1‑150 (MQ=255)
                                                                                                                                     aaaaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCatat          >  1:1413992/1‑150 (MQ=255)
                                                                                                                                          aTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGc     <  2:1155871/150‑1 (MQ=255)
                                                                                                                                           tGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACAAGATTTTCCAGTTGTTACTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCt    >  2:51772/1‑150 (MQ=255)
                                                                                                                                           ggCCCGGTTATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCt    >  2:751750/2‑150 (MQ=255)
                                                                                                                                            gCCCGGTTATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTa   >  1:356641/1‑150 (MQ=255)
                                                                                                                                            gCCCGGTTATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGATCCTGAGCATATTTGGCTa   >  2:1311461/1‑150 (MQ=255)
                                                                                                                                            gCCCGGTATCTCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTa   <  1:1009566/150‑1 (MQ=255)
                                                                                                                                             cccGGTTATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCCCTCTGAAGCACCGGAGACAAATGATGTTCATGAGCATATTTGGCTaa  >  2:219438/1‑150 (MQ=255)
                                                                                                                                             cccGGTTATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTaa  >  1:440899/1‑150 (MQ=255)
                                                                                                                                             cccGGTTATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCCTGATATGTCGAACAGAAAAAGGTTTACCATATTTTCCAGTTGTTCCTGGCGACCACTCAGATGCACCGGAGACAAAAGATGTTCAGTCGCTTATTTTGCTaa  >  2:724055/1‑150 (MQ=255)
                                                                                                                                             cccGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTaa  >  1:440619/1‑150 (MQ=255)
                                                                                                                                             cccGGAATCTCTACAGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTAACCAGATATTCCAGTTGTTCCTGCCGACCACTCTGATGAACAGGAGACAAATGATGTTCCTGAGCATATTTGGCTaa  >  2:493343/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAA  >  NC_000913/3729254‑3729544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: