Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,747,168:1 +C 7.7% coding (85/1497 nt) lyxK → L‑xylulose kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,747,1681.C 7.7% 291.9 / 22.0 78coding (85/1497 nt)lyxKL‑xylulose kinase
Reads supporting (aligned to +/- strand):  ref base . (39/33);  new base C (3/3);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

TGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCA  >  NC_000913/3747019‑3747315
                                                                                                                                                      |                                                                                                                                                   
tgtttgttAAAGATGTTCCGCAGGGAGCTGATCTGCTGAAAGCCGTTGATGATGTTGAATAAACCATGTCGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAATGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCg                                                                                                                                                      <  2:558621/150‑1 (MQ=255)
tgtttgttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCg                                                                                                                                                      >  2:222391/1‑150 (MQ=255)
tgtttgttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCg                                                                                                                                                      <  1:644272/150‑1 (MQ=255)
 gtttgttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑t                                                                                                                                                    >  2:1690648/1‑150 (MQ=255)
   ttgttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGc                                                                                                                                                  <  2:1244875/150‑1 (MQ=255)
    tgttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGGTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCa                                                                                                                                                 <  1:610490/150‑1 (MQ=255)
      ttAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAgc                                                                                                                                               >  1:1667381/1‑150 (MQ=255)
       tAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAgcg                                                                                                                                              <  2:449562/150‑1 (MQ=255)
           gATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTg                                                                                                                                          >  1:1311508/1‑150 (MQ=255)
             tgtgCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCA‑CGCCTGcc                                                                                                                                        <  1:1375606/150‑1 (MQ=255)
             tgtgCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCA‑CGCCTGcc                                                                                                                                        <  2:640663/150‑1 (MQ=255)
             tgtgCCGCAGGGAGCGGATCTGCGCACAGCCGTGGATGAGGTGCCATACACCATGCCGCAATACTGGCTGGTGTGAGTTTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGcc                                                                                                                                        <  2:478907/150‑1 (MQ=255)
                gCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGCGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCGCTGCA‑CGCCTGcctcc                                                                                                                                     <  1:422705/150‑4 (MQ=255)
                     cgggAGCTGATCTGCTGAAAGCCGTGGAAGAGGTGCAATAAACCATGACGCAAGTCTGGCTGGGGTTTGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTgcg                                                                                                                                <  2:503314/149‑1 (MQ=255)
                     aGGGAGCTGATCTGCTGAACGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑GGCAGCGCCTGCCGCTGTgcg                                                                                                                                <  2:1613803/150‑1 (MQ=255)
                      gggAGCGGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGGTAGATTGTGGCGGTAGCTGGCTGAACGCCGGGCGGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTgcgc                                                                                                                               <  1:697903/150‑1 (MQ=255)
                        gAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCAt                                                                                                                             <  1:1384073/150‑1 (MQ=255)
                        gAGCTGATCTGCTGAAAGCCGTGGATGAGGGGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCAt                                                                                                                             <  1:6117/150‑1 (MQ=255)
                            tGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAg                                                                                                                         >  1:346810/1‑150 (MQ=255)
                             gATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCTCTGTGCGCATTAAGc                                                                                                                        >  2:1263364/1‑150 (MQ=255)
                             gATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGc                                                                                                                        >  1:544913/1‑150 (MQ=255)
                                          ccGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCt                                                                                                           >  1:864413/1‑150 (MQ=255)
                                             tGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGTAGGCAGGCG‑TGAAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTcgg                                                                                                        >  1:1697007/1‑150 (MQ=255)
                                             tGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTggg                                                                                                        <  2:816038/150‑1 (MQ=255)
                                                   aGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCACATTAAGCCCGCAGCCAGGCTGGGCAGAgc                                                                                                  <  2:1632258/150‑1 (MQ=255)
                                                    ggTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAgcg                                                                                                 <  2:544913/150‑1 (MQ=255)
                                                      ggCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCGGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGcgcg                                                                                               <  2:197192/149‑1 (MQ=255)
                                                       gCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGa                                                                                              <  2:561821/150‑1 (MQ=255)
                                                        cAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGat                                                                                             <  1:222391/150‑1 (MQ=255)
                                                         aaTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGata                                                                                            >  2:636940/1‑150 (MQ=255)
                                                          aTAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGCGCGCGatat                                                                                           >  1:1074135/1‑150 (MQ=255)
                                                            aaaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGTTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATgg                                                                                         >  2:352767/1‑150 (MQ=255)
                                                             aaCCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCAAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCCCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGCGCGCGATATGGc                                                                                        >  1:662058/1‑150 (MQ=255)
                                                               ccATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAg                                                                                      >  2:931841/1‑150 (MQ=255)
                                                                             tggctggGGTTAGAGTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgc                                                                        <  1:535130/150‑1 (MQ=255)
                                                                                cgggggTTAGATTGTGGCGGTAGCTGGCGGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAAtgctgc                                                                     <  1:203704/148‑1 (MQ=255)
                                                                                  ggggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCAt                                                                   >  2:479752/1‑150 (MQ=255)
                                                                                    ggTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGTAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATgg                                                                 >  2:182882/1‑150 (MQ=255)
                                                                                     gTTAGATTGTGGCGTTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGc                                                                <  1:1142727/150‑1 (MQ=255)
                                                                                       tAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTg                                                              <  1:1054669/150‑1 (MQ=255)
                                                                                        aGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGt                                                             >  2:687062/1‑150 (MQ=255)
                                                                                           ttGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAt                                                          >  1:1474414/1‑150 (MQ=255)
                                                                                            tgtgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAtt                                                         <  1:11587/150‑1 (MQ=255)
                                                                                            tggggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCAtt                                                         <  1:1601901/147‑1 (MQ=255)
                                                                                             gtgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTc                                                        >  2:1094383/1‑150 (MQ=255)
                                                                                             gtgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATAAGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTc                                                        >  2:201668/1‑150 (MQ=255)
                                                                                              tgGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcg                                                       >  2:446645/1‑150 (MQ=255)
                                                                                              gggCGGTAGCTGGCGGAAAGCCGGGCGGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcg                                                       <  1:1311072/149‑1 (MQ=255)
                                                                                               ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcgc                                                      <  2:710341/150‑1 (MQ=255)
                                                                                               ggCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcgc                                                      <  2:864413/150‑1 (MQ=255)
                                                                                               ggCGGTAGCTGGCGGAAAGCCGGTCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcgc                                                      <  1:168159/150‑1 (MQ=255)
                                                                                                gCGGTAGATGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTcgcg                                                     >  1:1551501/1‑150 (MQ=255)
                                                                                                 cGGTAGTTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCgcgc                                                    <  1:352767/150‑1 (MQ=255)
                                                                                                  ggTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGcc                                                   <  2:1311508/150‑1 (MQ=255)
                                                                                                       ctggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCt                                              >  2:729912/1‑150 (MQ=255)
                                                                                                         ggctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTa                                            >  1:778035/1‑150 (MQ=255)
                                                                                                          gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGTG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTAc                                           >  2:1157319/1‑150 (MQ=255)
                                                                                                          gctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTAc                                           <  1:1690648/150‑1 (MQ=255)
                                                                                                           ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACt                                          >  2:479505/1‑150 (MQ=255)
                                                                                                           ctgAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAACCCCGAACCCAGGCTGGGCGGCGCGCCATATGGAAGACGTGTGCCAATCATGAAGGGCTGTCTTACGCGCCCTCATTACt                                          >  1:623059/1‑150 (MQ=255)
                                                                                                             gAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCa                                        >  1:904394/1‑150 (MQ=255)
                                                                                                               aaGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCAtt                                      >  2:1676780/1‑150 (MQ=255)
                                                                                                                aGCCGGGCTGTATGACCGCGAAGGCCGGGCGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGACCTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTc                                     >  1:1536265/1‑150 (MQ=255)
                                                                                                                 gCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCt                                    >  2:160654/1‑150 (MQ=255)
                                                                                                                   cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGTG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTgg                                  <  1:1157319/150‑1 (MQ=255)
                                                                                                                   cGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTgg                                  <  1:211760/150‑1 (MQ=255)
                                                                                                                         gTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCCGGCTGGGCCGCGCGCGAGATGGCAGCACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTGACTCATTCTGGTGTTAg                            >  1:120793/1‑150 (MQ=255)
                                                                                                                            tGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgg                         >  1:522543/1‑150 (MQ=255)
                                                                                                                            tGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCgg                         >  1:1635875/1‑150 (MQ=255)
                                                                                                                                cgcgAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGaa                     >  1:1664694/1‑150 (MQ=255)
                                                                                                                                 gcgAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAAc                    >  1:573053/1‑150 (MQ=255)
                                                                                                                                 gcgAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAAc                    >  1:692412/1‑150 (MQ=255)
                                                                                                                                   gAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACaa                  >  1:973426/1‑150 (MQ=255)
                                                                                                                                   gAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACaa                  >  1:948640/1‑150 (MQ=255)
                                                                                                                                     aGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAAt                <  2:1664694/150‑1 (MQ=255)
                                                                                                                                        cacagcggcaggcGCTGCA‑CGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGt             >  2:1240321/7‑150 (MQ=255)
                                                                                                                                         cGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTc            >  2:1182903/1‑150 (MQ=255)
                                                                                                                                           agcggcaggcGCTGCA‑CGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCATACTCATTCTGGTGTTAGCGGGGAACAAATTGTctg          >  1:1290387/4‑148 (MQ=255)
                                                                                                                                              ggcaggcGCTGCA‑CGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTAt       >  1:1231969/1‑150 (MQ=255)
                                                                                                                                              ggcaggcG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGATTACTCATTCTGGTGTTAGCGGGGAACATATTGTAGGTAt       >  2:864664/1‑150 (MQ=255)
                                                                                                                                                 aggcG‑GGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCgg    <  2:560503/150‑1 (MQ=255)
                                                                                                                                                   gcG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCa  <  1:729912/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TGTTTGTTAAAGATGTGCCGCAGGGAGCTGATCTGCTGAAAGCCGTGGATGAGGTGCAATAAACCATGACGCAATACTGGCTGGGGTTAGATTGTGGCGGTAGCTGGCTGAAAGCCGGGCTGTATGACCGCGAAGGCCGGGAGGCAGGCG‑TGCAGCGCCTGCCGCTGTGCGCATTAAGCCCGCAGCCAGGCTGGGCAGAGCGCGATATGGCAGAACTGTGGCAATGCTGCATGGCTGTCATTCGCGCCCTGCTTACTCATTCTGGTGTTAGCGGGGAACAAATTGTCGGTATCGGCA  >  NC_000913/3747019‑3747315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑