Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,062,186:1 +G 5.3% intergenic (+41/‑61) yihM → / → yihN putative sugar phosphate isomerase/MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,062,1861.G 5.3% 600.0 / 29.5 150intergenic (+41/‑61)yihM/yihNputative sugar phosphate isomerase/MFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base . (76/66);  new base G (6/2);  total (82/68)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.38e-01

Only 100 of 160 total aligned reads displayed.

GGATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTT  >  NC_000913/4062037‑4062335
                                                                                                                                                      |                                                                                                                                                     
ggATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAGACCATTCATATg                                                                                                                                                      >  2:638301/1‑150 (MQ=255)
 gATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATg                                                                                                                                                      >  1:61188/1‑150 (MQ=255)
   tGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGaa                                                                                                                                                    >  1:1391866/1‑150 (MQ=255)
         gTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCg                                                                                                                                              >  2:1642153/1‑150 (MQ=255)
         gTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCg                                                                                                                                              >  1:553206/1‑150 (MQ=255)
          ttACGTAAAGAAAAAGAAGATGCAATTTATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAGACAAGAGGCAACCCATCTTCTGAACCACTAATAAGTTTTAACTCAGCCGCAGGTAATAAAACCATTCCATTTGAATGGTCgg                                                                                                                                             >  2:148142/1‑150 (MQ=255)
          ttACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCgg                                                                                                                                             <  2:743202/150‑1 (MQ=255)
          ttACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTATAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCgg                                                                                                                                             >  2:1582482/1‑150 (MQ=255)
           tACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAGACCATTCATATGGAATGGtttta                                                                                                                                            <  1:638301/150‑5 (MQ=255)
           tACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCg                                                                                                                                              >  1:1714619/1‑148 (MQ=255)
             cGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTAt                                                                                                                                          >  2:1162189/1‑150 (MQ=255)
              gTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATc                                                                                                                                         >  1:494934/1‑150 (MQ=255)
              gTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATc                                                                                                                                         >  1:1423999/1‑150 (MQ=255)
                 aagaaaaagaaGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTa                                                                                                                                      <  1:452746/150‑1 (MQ=255)
                    aaaaagaaGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTg                                                                                                                                   >  2:492004/1‑150 (MQ=255)
                        agaagaTTCAATTAATCAGATAAATCATGTGCGTAAGTACTACAACAAATCAAAAAAGACGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATCTGAATGGTCGGTATCTTATTGCCttt                                                                                                                              <  1:253695/150‑1 (MQ=255)
                          aatattcAATTAAACAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTa                                                                                                                             <  1:1520567/144‑1 (MQ=255)
                          aagaTGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTa                                                                                                                             <  2:1363014/150‑1 (MQ=255)
                          aagaTGCAATTAATCAGATAAATCATGTGCGTAACGTACTAAAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTa                                                                                                                             <  2:163059/150‑1 (MQ=255)
                              tGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAAc                                                                                                                         >  1:1581054/1‑150 (MQ=255)
                               gCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACa                                                                                                                        <  2:366275/150‑1 (MQ=255)
                                cAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAg                                                                                                                       <  1:1606289/150‑1 (MQ=255)
                                  aTTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTg                                                                                                                     <  2:132988/150‑1 (MQ=255)
                                      aTCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGAttt                                                                                                                 >  2:1104296/1‑150 (MQ=255)
                                      aTCAGATAAATCATGTGCGTAAAGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGAttt                                                                                                                 <  1:1649739/150‑1 (MQ=255)
                                        cAGATAAATCATGTGCGTAACGTACTTCAACAAATCAAACAAGAGGCAAACCATCTACTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTa                                                                                                               >  2:1010172/1‑150 (MQ=255)
                                           aTAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTaaaa                                                                                                            <  1:997362/150‑1 (MQ=255)
                                                 cATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCt                                                                                                      >  2:838494/1‑150 (MQ=255)
                                                  atttgCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTATCTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCtt                                                                                                     <  1:407884/147‑1 (MQ=255)
                                                  aTGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCtt                                                                                                     <  2:486321/150‑1 (MQ=255)
                                                    gtgCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAg                                                                                                   >  1:302632/1‑150 (MQ=255)
                                                    gtgCGTAACGTACTACAACAATCAAACAAGAGGCTAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAgg                                                                                                  <  1:1311601/150‑1 (MQ=255)
                                                      gCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAggg                                                                                                 <  2:1486627/150‑1 (MQ=255)
                                                       cGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAgggg                                                                                                <  1:1337845/150‑1 (MQ=255)
                                                       cGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAgggg                                                                                                >  2:1650327/1‑150 (MQ=255)
                                                        ttAACGTACTACAAAAAATCAAACAATAGGCAAACAATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGa                                                                                               <  1:1470883/149‑1 (MQ=255)
                                                        gTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGa                                                                                               >  2:1003624/1‑150 (MQ=255)
                                                         tAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGAt                                                                                              <  2:665805/150‑1 (MQ=255)
                                                           aCGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGt                                                                                            <  2:713826/150‑1 (MQ=255)
                                                             gTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTa                                                                                          <  1:216398/150‑1 (MQ=255)
                                                             gTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTa                                                                                          >  1:1110093/1‑150 (MQ=255)
                                                             gTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTa                                                                                          <  1:650091/150‑1 (MQ=255)
                                                             gTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGGCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTa                                                                                          <  1:1052509/150‑1 (MQ=255)
                                                              tactacAACAAATCAAACAATAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTAt                                                                                         <  1:1162189/150‑1 (MQ=255)
                                                              tactacAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTAt                                                                                         >  1:1241004/1‑150 (MQ=255)
                                                               actacAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATg                                                                                        <  1:411248/150‑1 (MQ=255)
                                                               actacAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATg                                                                                        >  2:1430120/1‑150 (MQ=255)
                                                                ctacAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCAAACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGc                                                                                       >  2:785917/1‑150 (MQ=255)
                                                                   caacaaATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCa                                                                                    >  2:1424874/1‑150 (MQ=255)
                                                                   aaacaaATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCa                                                                                    <  1:265806/149‑1 (MQ=255)
                                                                    aacaaATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCAc                                                                                   <  2:61188/150‑1 (MQ=255)
                                                                     acaaATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACg                                                                                  >  1:806417/1‑150 (MQ=255)
                                                                     acaaATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACg                                                                                  <  1:945020/150‑1 (MQ=255)
                                                                          tCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGaaaaa                                                                             >  1:1212744/1‑150 (MQ=255)
                                                                            aaaCAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATGGCTTAGGGGATGTTATGCTCACGAAAAAGa                                                                           >  1:874146/1‑150 (MQ=255)
                                                                                 agagGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATgg                                                                      >  2:30884/1‑150 (MQ=255)
                                                                                  gagGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTATTAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATggg                                                                     <  1:1171189/150‑1 (MQ=255)
                                                                                    ggCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCg                                                                   >  1:1596558/1‑150 (MQ=255)
                                                                                     gCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGt                                                                  <  2:1156982/150‑1 (MQ=255)
                                                                                           cATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGATTAGGGGATGTTATGCTCACGAAAAAGAAATGGGGTTTAtttt                                                            >  2:341466/1‑149 (MQ=255)
                                                                                               ttctGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCt                                                        <  2:226574/150‑1 (MQ=255)
                                                                                                tctGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTa                                                       >  2:912247/1‑150 (MQ=255)
                                                                                                  tGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTAtt                                                     >  1:1353118/1‑150 (MQ=255)
                                                                                                  tGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTAtt                                                     >  2:1576012/1‑150 (MQ=255)
                                                                                                     aCCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAac                                                  <  2:16109/150‑1 (MQ=255)
                                                                                                      ccACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAaca                                                 >  1:885440/1‑150 (MQ=255)
                                                                                                        aCTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTaa                                                   >  2:1181132/1‑146 (MQ=255)
                                                                                                        aCTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTaa                                                   <  1:1181132/146‑1 (MQ=255)
                                                                                                         cTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTTAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACtg                                              <  2:1246972/150‑1 (MQ=255)
                                                                                                                gTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCg                                       >  1:1250961/1‑150 (MQ=255)
                                                                                                                   ttAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTTATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTa                                    >  2:501308/1‑150 (MQ=255)
                                                                                                                        tCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAAtt                               <  2:1241004/150‑1 (MQ=255)
                                                                                                                           tACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTTTGGCGGTACAATTtat                            >  1:1630937/1‑150 (MQ=255)
                                                                                                                            aCGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTtata                           <  1:351313/150‑1 (MQ=255)
                                                                                                                            aCGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTAAGGGGATGTTATGCTCAAGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTtata                           >  1:450145/1‑150 (MQ=255)
                                                                                                                             cGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATaa                          >  2:876838/1‑150 (MQ=255)
                                                                                                                              gCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATaaa                         >  2:157559/1‑150 (MQ=255)
                                                                                                                               gaggtaattaAACCATTCCATATGAATGGTAGGTAGGTTATTGGCTTTAAAACAGTGATTTTAAAAATGGCTTAGGGGATGTTATGCTCCCGAAAAAGCAATGGGCGTTATTTTGTCTTTTAAACCTGTGTGGCGGTACAATTTATAAAt                        >  2:1364338/5‑150 (MQ=255)
                                                                                                                                   taataaAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTAcc                    <  2:1073265/150‑1 (MQ=255)
                                                                                                                                   taataaAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGATCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTAcc                    >  1:822627/1‑150 (MQ=255)
                                                                                                                                     taccgaCCATTCATATGGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATaaa                         >  1:1545176/6‑143 (MQ=255)
                                                                                                                                     ataaAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGt                  <  1:939615/150‑1 (MQ=255)
                                                                                                                                      taaAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTtata                           <  2:200804/140‑1 (MQ=255)
                                                                                                                                       ccgaCCATTCATATGGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCg                >  1:666089/4‑150 (MQ=255)
                                                                                                                                       ccgaCCATTCATATGGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCg                >  2:205860/4‑150 (MQ=255)
                                                                                                                                       ccgaCCATTCATATGGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCg                >  1:915356/4‑150 (MQ=255)
                                                                                                                                       aaaaCCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCg                <  2:1423999/150‑1 (MQ=255)
                                                                                                                                         aaCCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCt              <  1:1650327/150‑1 (MQ=255)
                                                                                                                                          aCCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTg             >  1:1655975/1‑150 (MQ=255)
                                                                                                                                            cATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGaa           <  1:1634783/150‑1 (MQ=255)
                                                                                                                                            cATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGaa           >  1:1128279/1‑150 (MQ=255)
                                                                                                                                               tCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGa        >  2:1281119/1‑150 (MQ=255)
                                                                                                                                                ccATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGAt       >  2:871683/1‑150 (MQ=255)
                                                                                                                                                 cATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATg      >  1:16270/1‑150 (MQ=255)
                                                                                                                                                 cATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATg      <  2:359348/150‑1 (MQ=255)
                                                                                                                                                 cATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATg      >  2:1687006/1‑150 (MQ=255)
                                                                                                                                                   tatGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCg    >  2:1282220/1‑150 (MQ=255)
                                                                                                                                                   tatGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCg    >  1:1667835/1‑150 (MQ=255)
                                                                                                                                                     tGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGtt  <  1:1010172/150‑1 (MQ=255)
                                                                                                                                                     tGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTAAAAATTACCGTCGCTGAAAGATGCGtt  >  1:1309724/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GGATGCGCGGTTACGTAAAGAAAAAGAAGATGCAATTAATCAGATAAATCATGTGCGTAACGTACTACAACAAATCAAACAAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTT  >  NC_000913/4062037‑4062335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: