Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,429,133:1 +C 8.4% coding (199/621 nt) fklB → FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,429,1331.C 8.4% 452.3 / 40.1 119coding (199/621 nt)fklBFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
Reads supporting (aligned to +/- strand):  ref base . (58/51);  new base C (3/7);  total (61/58)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.45e-01

Only 100 of 119 total aligned reads displayed.

GGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGC  >  NC_000913/4428986‑4429278
                                                                                                                                                     |                                                                                                                                                 
ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGTTGCATCGCGCGCTGCGTGAAATCCACGAg                                                                                                                                                 >  1:356110/1‑150 (MQ=255)
ggTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAg                                                                                                                                                 <  2:1070994/150‑1 (MQ=255)
 gTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGATGCCAGAAGCAAAGGTTGCAGGTATTGCAGATGAGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGGTGCGTGAAATCCACGAgc                                                                                                                                                >  2:303390/1‑150 (MQ=255)
  tttGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAgcg                                                                                                                                               <  1:397886/150‑1 (MQ=255)
  tttCCAGGGAGGACAACAACTGAGTGAATCTGGCCTTGAAGGGCTGCTTCCAGAAGCACTGGTAGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATCTGCTTCATCGCGCGCTGCGTGAAATCCACGAgcg                                                                                                                                               <  1:296461/150‑1 (MQ=255)
  gttGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAgcg                                                                                                                                               <  1:399422/149‑1 (MQ=255)
     gCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAgcgcg                                                                                                                                             <  1:414931/150‑1 (MQ=255)
     gCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAgcgcgc                                                                                                                                            >  2:1079787/1‑150 (MQ=255)
        ggTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGa                                                                                                                                         >  1:626034/1‑150 (MQ=255)
        ggTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGa                                                                                                                                         >  1:462141/1‑150 (MQ=255)
         gTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAgcgcgcgat                                                                                                                                         <  2:319180/150‑4 (MQ=38)
         gTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAgcgcgcgat                                                                                                                                         <  1:1229954/150‑4 (MQ=38)
          tAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGGATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGGCGCGCTCGTGGATTTCACGCAgcgcgcgatg                                                                                                                                        <  1:286897/150‑5 (MQ=37)
             ggCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCg                                                                                                                                    >  1:530391/1‑150 (MQ=255)
              gCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGAt                                                                                                                                        >  2:1692119/1‑145 (MQ=255)
              gCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGAt                                                                                                                                        <  1:1692119/145‑1 (MQ=255)
              gCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGt                                                                                                                                   >  1:1364270/1‑150 (MQ=255)
               caacaaCTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGtt                                                                                                                                  >  1:768040/1‑150 (MQ=255)
                  caacTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGCGCGCGCCGATGCCGTtcgt                                                                                                                               >  1:1705443/1‑150 (MQ=255)
                    acTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcgtcg                                                                                                                             >  2:982710/1‑150 (MQ=255)
                    acTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCAGATGCGCTGGAAGCCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGAGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTtcgacg                                                                                                                             >  2:349064/1‑150 (MQ=255)
                          tGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCg                                                                                                                       <  1:399464/150‑1 (MQ=255)
                               cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                                  <  1:1673284/150‑1 (MQ=255)
                               cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                                  <  2:792287/150‑1 (MQ=255)
                               cTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTcc                                                                                                                  >  1:505154/1‑150 (MQ=255)
                                tGGCCTGGAAGGGCTGCTGCCAGACGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCCAACATCCGGCTGTTCCGGTTGATGTGGTGCCTCGCGCGCTGCGGGCAATCCACGAGCGCGCCGCTGCCGTTCGGCGTCCGCGTTTCCa                                                                                                                 >  1:145551/1‑150 (MQ=255)
                                  gCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAgg                                                                                                               <  1:391987/150‑1 (MQ=255)
                                   ccTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCATCGTCAGTGTTTCCAGGc                                                                                                              >  1:631631/1‑150 (MQ=255)
                                       gacgggCGGCTGGCAGAAGCTCTGGTTGCAGGTTTTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATg                                                                                                          <  2:130843/147‑1 (MQ=255)
                                         cgggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgc                                                                                                        <  2:1560993/149‑1 (MQ=255)
                                         aGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgc                                                                                                        >  2:439076/1‑150 (MQ=255)
                                           ggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctg                                                                                                      <  2:462141/150‑1 (MQ=255)
                                           ggCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGgctg                                                                                                      >  2:1089415/1‑150 (MQ=255)
                                              tgctgcCAGAAGCACTGGTTGCGGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGctgctg                                                                                                   <  2:642872/150‑1 (MQ=255)
                                                  gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                               >  2:752380/1‑150 (MQ=255)
                                                  gcCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAgg                                                                                               >  1:1280331/1‑150 (MQ=255)
                                                    cAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtg                                                                                             >  2:281625/1‑150 (MQ=255)
                                                    cAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGgtg                                                                                             <  1:1241451/150‑1 (MQ=255)
                                                      gAAGCACTGGGTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGtgtg                                                                                           <  1:900180/150‑1 (MQ=255)
                                                          cACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAt                                                                                       >  1:104040/1‑150 (MQ=255)
                                                           aCTGGTTGCATGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGACGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATa                                                                                      <  2:284101/150‑1 (MQ=255)
                                                            cTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGAGAAATAc                                                                                     >  1:63210/1‑150 (MQ=255)
                                                             tGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATAcc                                                                                    >  1:1314704/1‑150 (MQ=255)
                                                               gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                                  <  2:1439278/150‑1 (MQ=255)
                                                               gTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTg                                                                                  <  2:768040/150‑1 (MQ=255)
                                                                 tGCAGGTTTTGGCGATGCGCTGGAAGGCAAACATCCGGCTGTTCGGGTTGATGTTGTGCATCGCGCGCTGCGGGAAATCCACGAGCGCGCCGATGGCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGga                                                                                <  2:214307/150‑1 (MQ=255)
                                                                 tGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTTGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGga                                                                                <  1:1320957/150‑1 (MQ=255)
                                                                 tGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGga                                                                                >  2:1432243/1‑150 (MQ=255)
                                                                 tGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGga                                                                                >  2:1679108/1‑150 (MQ=255)
                                                                  gCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaa                                                                               >  1:1358360/1‑150 (MQ=255)
                                                                   cAGGGATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGgaag                                                                              <  1:929610/150‑1 (MQ=255)
                                                                       tATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGaaaa                                                                          >  1:780283/1‑150 (MQ=255)
                                                                       tATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGGGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGaaaa                                                                          <  2:881370/150‑1 (MQ=255)
                                                                        aTTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAAc                                                                         <  1:982710/150‑1 (MQ=255)
                                                                        aTTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATAACTGGAAGAAAAc                                                                         >  2:750149/1‑150 (MQ=255)
                                                                         ttGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACg                                                                        <  2:1650514/150‑1 (MQ=255)
                                                                           gCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGcc                                                                      >  1:1417692/1‑150 (MQ=255)
                                                                           gCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGcc                                                                      >  1:743494/1‑150 (MQ=255)
                                                                            ccGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCa                                                                     >  1:179375/1‑150 (MQ=255)
                                                                              gATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCCGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCaaa                                                                   >  1:1096516/1‑150 (MQ=255)
                                                                                  cgcTGGATGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaag                                                               >  2:1082300/1‑150 (MQ=255)
                                                                                   gcTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaaga                                                              >  1:795422/1‑150 (MQ=255)
                                                                                    cTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaa                                                             <  1:1079787/150‑1 (MQ=255)
                                                                                     tGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAaagaag                                                            <  2:1406003/150‑1 (MQ=255)
                                                                                      ggAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAgg                                                           >  1:527845/1‑150 (MQ=255)
                                                                                      ggAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAgg                                                           <  1:366179/150‑1 (MQ=255)
                                                                                      ggAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTCCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAgg                                                           <  1:911636/150‑1 (MQ=255)
                                                                                      ggAAGGCAAACATCCGGCTGTTCCGGGTGATGTGGTGCATCGCGCGCTGCGTGCAATCCACGCGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGCAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAgg                                                           >  1:1483878/1‑150 (MQ=255)
                                                                                          ggCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                       <  1:732007/150‑1 (MQ=255)
                                                                                            cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCTATGGCTGCTGAAGGTGTGAAATACCTgg                                                                                 <  1:553971/122‑1 (MQ=255)
                                                                                            cAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTgg                                                                                 >  2:553971/1‑122 (MQ=255)
                                                                                             aaaCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAAt                                                    <  2:1280331/150‑1 (MQ=255)
                                                                                              aaCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATa                                                   >  1:1264924/1‑150 (MQ=255)
                                                                                               aCATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAg                                                  <  1:752380/150‑1 (MQ=255)
                                                                                                cATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCTAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGc                                                 >  2:815227/1‑150 (MQ=255)
                                                                                                cATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGc                                                 <  2:63210/150‑1 (MQ=255)
                                                                                                    cGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCg                                             >  2:452715/1‑150 (MQ=255)
                                                                                                            ccGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGc                                     >  1:352701/1‑150 (MQ=255)
                                                                                                            ccGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGc                                     <  1:1467361/150‑1 (MQ=255)
                                                                                                             cGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGcc                                    >  2:1555703/1‑150 (MQ=255)
                                                                                                                gtGATGTGGTGCATCGCGCGCTGCGTGAATTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGc                                 <  1:303390/149‑1 (MQ=255)
                                                                                                                   aTGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAACCGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAAt                              >  1:1282602/1‑150 (MQ=255)
                                                                                                                    tgtgGTGCATCGCGCGCTGCGTGCAATCCACGAGCGCGCGGATGCCGTTCGTCGTCAGCGTGTCCAGGGGATGGCTGCTGAAGGTGTGATATACCTGGAAGATAACGCCAAAAAAGAAGGTGTGTATAGCACCGAATCTGGGCTGCACtt                             >  1:223792/1‑150 (MQ=255)
                                                                                                                      tggtgCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTcc                           >  1:138041/1‑150 (MQ=255)
                                                                                                                         tgCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCcgcg                        >  1:1592321/1‑150 (MQ=255)
                                                                                                                          gCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                       <  2:899392/119‑1 (MQ=37)
                                                                                                                          gCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAGACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                       >  1:905497/1‑119 (MQ=37)
                                                                                                                          gCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAAGACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                       >  1:899392/1‑119 (MQ=37)
                                                                                                                          gCATCGGCGCGCTCGTGGATTTCACGCAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAACACCTGGAAGAAAACGCCAAAAAAGAAGGtgtg                                                       <  2:905497/119‑1 (MQ=37)
                                                                                                                              cgcgcgCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATc                   <  2:505154/150‑1 (MQ=255)
                                                                                                                                gcgcgcTCGTGGATTTCACGCAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCa                  >  2:69410/1‑150 (MQ=255)
                                                                                                                                 cgcgcTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGATGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCaa                 >  2:929339/1‑150 (MQ=255)
                                                                                                                                      tGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAgg            >  2:372921/1‑150 (MQ=255)
                                                                                                                                      ggCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAgg            <  1:676102/149‑1 (MQ=255)
                                                                                                                                       gCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAggg           <  2:626034/150‑1 (MQ=255)
                                                                                                                                       gCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAggg           <  1:1089415/150‑1 (MQ=255)
                                                                                                                                         gTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTg         >  1:1063269/1‑150 (MQ=255)
                                                                                                                                             aaTCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAgg     <  1:1432243/150‑1 (MQ=255)
                                                                                                                                                ccACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCTTGCAATTCCGCGTGATCAACCAGGGTGAAGgcgc  <  2:104040/150‑1 (MQ=255)
                                                                                                                                                ccACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGgcgc  <  1:1164799/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GGTTTGCAGGTAGGGCAACAACTGAGTGAATCTGGCCTGGAAGGGCTGCTGCCAGAAGCACTGGTTGCAGGTATTGCCGATGCGCTGGAAGGCAAACATCCGGCTGTTCCGGTTGATGTGGTGCATCGCGCGCTGCGTGAAATCCACGAGCGCGCCGATGCCGTTCGTCGTCAGCGTTTCCAGGCGATGGCTGCTGAAGGTGTGAAATACCTGGAAGAAAACGCCAAAAAAGAAGGTGTGAATAGCACCGAATCTGGCCTGCAATTCCGCGTGATCAACCAGGGTGAAGGCGC  >  NC_000913/4428986‑4429278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: