Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 922,314:1 +C 7.3% intergenic (+21/+52) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913922,3141.C 7.3% 410.8 / 30.8 110intergenic (+21/+52)macB/cspDmacrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
Reads supporting (aligned to +/- strand):  ref base . (47/55);  new base C (3/5);  total (50/60)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.47e-01

Only 100 of 111 total aligned reads displayed.

GGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTT  >  NC_000913/922168‑922463
                                                                                                                                                   |                                                                                                                                                     
ggTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCg                                                                                                                                                    >  2:570683/1‑150 (MQ=255)
    tttCACCGTTGGGGCTGCTGGTGGCGTTTGTCTGCTCGACGGGCACGGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCg                                                                                                                                                <  2:1532570/150‑1 (MQ=255)
    tttCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCg                                                                                                                                                >  1:197364/1‑150 (MQ=255)
    tttCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCg                                                                                                                                                <  2:561324/150‑1 (MQ=255)
     ttCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCgg                                                                                                                                               >  2:960027/1‑150 (MQ=255)
     ttCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCgg                                                                                                                                               >  1:1608572/1‑150 (MQ=255)
       cACCGTTGGCGCTGCTGCTGGCGTTTCGCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGAACCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGc                                                                                                                                             <  2:406993/150‑1 (MQ=255)
        aCCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCt                                                                                                                                            >  1:1083081/1‑150 (MQ=255)
         ccGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGCCTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCATCGGGCTg                                                                                                                                           >  1:186185/1‑150 (MQ=255)
         ccGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTg                                                                                                                                           <  1:941790/150‑1 (MQ=255)
           gTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGc                                                                                                                                         <  2:807370/150‑1 (MQ=255)
            ttGGCGCTGCTGCTGTCGTTTAGCTGCTCGACGGGCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCTCGACTGGATCTAGTAGATGCTCTGGCACTAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCa                                                                                                                                        <  1:1066162/150‑1 (MQ=255)
            ttGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCa                                                                                                                                        <  2:1312291/150‑1 (MQ=255)
             tGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAt                                                                                                                                       <  2:636862/150‑1 (MQ=255)
              ggCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAtt                                                                                                                                      <  1:746842/150‑1 (MQ=255)
              ggCGCTGCTGCTGGCGTTTCTCTGCTCGACGGGCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAtt                                                                                                                                      <  1:987884/150‑1 (MQ=255)
              ggCGCTGCTGCTGGCGGTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtt                                                                                                                                      <  2:710941/150‑1 (MQ=255)
                cgcTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCAtttt                                                                                                                                    <  2:1161508/150‑1 (MQ=255)
                 gctgctgctGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTg                                                                                                                                   <  2:610087/150‑1 (MQ=255)
                    gctgctgGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCAAGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCt                                                                                                                                >  1:280758/1‑150 (MQ=255)
                    gctgctgGCGCTTCTCTGCTCGACGGTCACCGGGATTTTAATTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCCGATCGGCTGGCAtttttatc                                                                                                                                <  1:495584/150‑5 (MQ=255)
                     ctgctgGCGTTTCTCTGCTCGCCGGTCACCGGGATTTTATTAGGCTGGTTACCCGCACGAAATGCGGCCCGACTGGATCCAGTAGATGCTCTGCCAGGAGCGTAATTTTTGAGATAAAAATGCCAGCCGATCGGCCTGGCCTTTTGCGtt                                                                                                                               >  2:1162692/1‑150 (MQ=255)
                          ggCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGa                                                                                                                          >  1:1622025/1‑150 (MQ=255)
                            cGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATg                                                                                                                        <  1:336125/150‑1 (MQ=255)
                              tttCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTa                                                                                                                      <  2:1608572/150‑1 (MQ=255)
                                tctcTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACTAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTaca                                                                                                                    <  2:1453851/150‑1 (MQ=255)
                                tctcTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTaca                                                                                                                    <  1:1176129/150‑1 (MQ=255)
                                 ctctGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTacac                                                                                                                   >  2:1258044/1‑150 (MQ=255)
                                 ctctGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTacac                                                                                                                   >  1:831929/1‑150 (MQ=255)
                                    tGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAAt                                                                                                                <  1:1708579/150‑1 (MQ=255)
                                            ggTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACaga                                                                                                        <  1:1258044/150‑1 (MQ=255)
                                               cACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGCCTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCC‑‑TAGGATGTACACAATGAGACAGAAGAGc                                                                                                   >  1:258495/1‑150 (MQ=255)
                                               cACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACagaaga                                                                                                     >  1:1460035/1‑150 (MQ=255)
                                                aCCGGGATTTTATTTGGCTGGTTACCTGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGATAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAagag                                                                                                    <  1:1365267/150‑1 (MQ=255)
                                                aCCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAagag                                                                                                    <  1:459013/150‑1 (MQ=255)
                                                aCCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAagag                                                                                                    >  1:718544/1‑150 (MQ=255)
                                                aCCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAagag                                                                                                    >  2:414113/1‑150 (MQ=255)
                                                 ccGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGc                                                                                                   <  1:960027/150‑1 (MQ=255)
                                                   gggATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCATGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCATTTAGGATGTACACAATGAGACAGAAGAGCTa                                                                                                 >  2:781419/1‑150 (MQ=255)
                                                    ggATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTAt                                                                                                >  1:1689372/1‑150 (MQ=255)
                                                       ttttATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCg                                                                                             >  2:1224111/1‑150 (MQ=255)
                                                            tttGGCTTGTTACCCGAACGAAATGCGGCAAGACTGGATCCAGTAGATGCGCTGTCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGc                                                                                        <  2:265624/150‑1 (MQ=255)
                                                            tttGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGc                                                                                        <  2:975780/150‑1 (MQ=255)
                                                            tttGGCGTGTTCCACGCACGAAAGGCGGCACGACTGGATCCAGTAGCTGCTCTTGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCAGATCGGGCTGGCATTTTGCCTTTAGGATGTCCACACTGAGACAGAAGCGCTATGCGACTGc                                                                                        <  2:269732/150‑1 (MQ=255)
                                                             ttGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGcc                                                                                       <  2:1637188/150‑1 (MQ=255)
                                                             ttGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGcc                                                                                       <  2:1598906/150‑1 (MQ=255)
                                                                gCTGGTTACCCGCACGCAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCt                                                                                    >  1:451594/1‑150 (MQ=255)
                                                                    gatACCCGCACGAACTGCGGCACGACTGGATCCAGTAGCTGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTa                                                                                <  1:597354/148‑1 (MQ=255)
                                                                       ccccGCTCGAAAGGCGGCACGATTGGATCCAGTAGATGCTTTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGACTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACtt                                                                             <  2:837681/149‑1 (MQ=255)
                                                                       aCCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACtt                                                                             >  2:717221/1‑150 (MQ=255)
                                                                        cccGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTc                                                                            >  1:1216378/1‑150 (MQ=255)
                                                                          cGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCCGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGa                                                                          >  1:236503/1‑150 (MQ=255)
                                                                                  aTGCGGCACGACGGCATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCATTCGGATGTACACAATGAGCCACAACAGCTATCCGACTGCCGCTTCTACTTCGACGGGcaca                                                                  <  2:223255/150‑1 (MQ=255)
                                                                                     cGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTTGCATTTTGCCTTTAGGATGTTCACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCGCAATa                                                               <  1:160650/150‑1 (MQ=255)
                                                                                      ggCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGCAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATaa                                                              >  1:852900/1‑150 (MQ=255)
                                                                                      ggCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATaa                                                              >  1:1542382/1‑150 (MQ=255)
                                                                                         aCGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAacac                                                           <  2:1083081/150‑1 (MQ=255)
                                                                                          cGACTGTATACAGTAGATGCTCTGGCACGAGAGTAAATTTTGAGATAAAAATTCCAGCCGAACGGGCTCGCAATTAGCCTTTAGGATCTACACAATGAGACAGAAGAGCAATGCGACTCCCGCTTCTACTTCGACGGGCACAATAacaca                                                          >  2:1450771/1‑149 (MQ=255)
                                                                                            aCTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTgg                                                        <  2:802319/150‑1 (MQ=255)
                                                                                              tGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAATATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCg                                                      <  2:649434/150‑1 (MQ=255)
                                                                                              tGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCg                                                      >  1:1185071/1‑150 (MQ=255)
                                                                                              tGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCg                                                      >  1:1507390/1‑150 (MQ=255)
                                                                                                gATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTg                                                    >  2:1716276/1‑150 (MQ=255)
                                                                                                    cAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGAttt                                                >  2:322319/1‑149 (MQ=255)
                                                                                                         gATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCttt                                           <  2:1231349/150‑1 (MQ=255)
                                                                                                            tctctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTgg                                        <  2:1333246/149‑1 (MQ=255)
                                                                                                            gcgctGGCACGAGAGTAATTTTTGATATAATAATGCCAGCTGATCGGGCGGGCATTTTGGTTTTAGGATGTACTCAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTgg                                        <  2:280758/147‑1 (MQ=255)
                                                                                                             ctctGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGc                                       <  1:1377029/150‑1 (MQ=255)
                                                                                                                tGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACACTGAGACAGAAGCGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGcccc                                    <  1:1630693/150‑1 (MQ=255)
                                                                                                                   cACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAATAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtgg                                 <  2:1228610/150‑1 (MQ=255)
                                                                                                                   cACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtgg                                 >  2:1214304/1‑150 (MQ=255)
                                                                                                                    aagagaGTAATTTTTGAGATAAAAATGACAGCCGATCGGGCTGGCATTTTGCCTTTAGTATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggt                                >  2:643920/3‑150 (MQ=255)
                                                                                                                     cGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggtg                               <  2:1622025/150‑1 (MQ=255)
                                                                                                                     cGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTAAGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggtg                               <  1:1532930/150‑1 (MQ=255)
                                                                                                                      gagaGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCtggtgg                              >  2:23439/1‑150 (MQ=255)
                                                                                                                       agagTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGa                             >  2:601300/1‑150 (MQ=255)
                                                                                                                        gagTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGAc                            <  2:831929/150‑1 (MQ=255)
                                                                                                                         agTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACa                           <  1:1716276/150‑1 (MQ=255)
                                                                                                                            aaTTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCa                        <  2:1216378/150‑1 (MQ=255)
                                                                                                                             aTTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCC‑‑TAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAAc                     <  2:258495/150‑1 (MQ=255)
                                                                                                                              tttttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCaaa                      >  2:455332/1‑150 (MQ=255)
                                                                                                                               ttttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCaaaa                     >  1:1047948/1‑149 (MQ=255)
                                                                                                                               ttttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAAc                     >  1:528749/1‑150 (MQ=255)
                                                                                                                               ttttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAAc                     <  2:1460035/150‑1 (MQ=255)
                                                                                                                                tttGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACt                    <  1:717221/150‑1 (MQ=255)
                                                                                                                                   gagaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGaa                 >  1:1288595/1‑150 (MQ=255)
                                                                                                                                     gaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACg               <  1:1243350/150‑1 (MQ=255)
                                                                                                                                     gaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACg               <  2:1689372/150‑1 (MQ=255)
                                                                                                                                     gaTAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGACGCTTATACTTCGACGGGCACAATAACACTGGCGTGATTGCCGTTTGGCCCCTGGTGGACATCAAACTGAACg               >  1:284656/1‑150 (MQ=255)
                                                                                                                                         aaaaTGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACAATGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGACCGGAtt           >  2:1526082/1‑150 (MQ=255)
                                                                                                                                          aaaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTg          >  2:1644448/1‑150 (MQ=255)
                                                                                                                                           aaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGCCGGGCACAATAACACTGGCGTGATTGACTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGt         >  2:1623228/1‑150 (MQ=255)
                                                                                                                                           aaTGCCAGCCCGATCGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGt         >  1:1651315/1‑150 (MQ=255)
                                                                                                                                           aaTGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGt         >  1:418997/1‑150 (MQ=255)
                                                                                                                                             tGCCAGCGGATCGGGCTGGCTTTTTGACTTTAGGATGTACACAATGAGACCGTAGAGCTATGCGACTGCCGCTTCTACTTCGACTGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTcc       <  2:186185/150‑1 (MQ=255)
                                                                                                                                             tGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTcc       <  2:1507390/150‑1 (MQ=255)
                                                                                                                                             tGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTcc       <  2:1185071/150‑1 (MQ=255)
                                                                                                                                               ccAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAg     >  1:1719534/1‑150 (MQ=255)
                                                                                                                                                 aGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCt   <  2:906621/150‑1 (MQ=255)
                                                                                                                                                  gCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAAAGGATTGTCCAGCtt  >  1:1250680/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGTTTTTCACCGTTGGCGCTGCTGCTGGCGTTTCTCTGCTCGACGGTCACCGGGATTTTATTTGGCTGGTTACCCGCACGAAATGCGGCACGACTGGATCCAGTAGATGCTCTGGCACGAGAGTAATTTTTGAGATAAAAATGCCAGCCGATCGGGCTGGCATTTTGCCTTTAGGATGTACACAATGAGACAGAAGAGCTATGCGACTGCCGCTTCTACTTCGACGGGCACAATAACACTGGCGTGATTGCCTTTTGGCCCCTGGTGGACATCAAACTGAACGGATTGTCCAGCTT  >  NC_000913/922168‑922463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: