Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,299,267:1 +A 7.6% intergenic (+22/‑1915) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,299,2671.A 7.6% 383.3 / 29.1 105intergenic (+22/‑1915)ychE/oppAUPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base . (56/41);  new base A (5/3);  total (61/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

Only 100 of 105 total aligned reads displayed.

GTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTC  >  NC_000913/1299120‑1299415
                                                                                                                                                    |                                                                                                                                                    
gTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTg                                                                                                                                                    <  2:1362084/150‑1 (MQ=255)
     ggTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATg                                                                                                                                               >  1:741311/1‑150 (MQ=255)
      gTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCGAATGAAACGGAGCTGCCATGc                                                                                                                                              <  2:1431625/150‑1 (MQ=255)
            cGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCATGGCAGCTCCGtt                                                                                                                                        <  1:487261/150‑1 (MQ=255)
            cGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCATGGCAGCTCCGtt                                                                                                                                        <  2:792123/150‑1 (MQ=255)
            cGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGtt                                                                                                                                        >  2:1069966/1‑150 (MQ=255)
             gCCAGACCGGCATCACAGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGttt                                                                                                                                       <  1:1610040/150‑1 (MQ=255)
             gCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGttt                                                                                                                                       >  1:719642/1‑150 (MQ=255)
              ccAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTa                                                                                                                                      >  2:1103447/1‑150 (MQ=255)
               cAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTAc                                                                                                                                     >  1:1631526/1‑150 (MQ=255)
               cAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGATCCGTTTAc                                                                                                                                     >  2:772646/1‑150 (MQ=255)
                   ccGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCATGGCAGCTCCGtttcatttg                                                                                                                                 <  1:1164664/150‑7 (MQ=255)
                   ccGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCg                                                                                                                                 >  2:363131/1‑150 (MQ=255)
                   ccGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCATTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCg                                                                                                                                 >  2:1330085/1‑150 (MQ=255)
                     ggCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTc                                                                                                                               <  1:786560/150‑1 (MQ=255)
                       cATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCat                                                                                                                             <  1:564506/150‑1 (MQ=255)
                       cATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCat                                                                                                                             <  1:910043/150‑1 (MQ=255)
                        aTCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCatt                                                                                                                            >  2:667629/1‑150 (MQ=255)
                        aTCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCatt                                                                                                                            <  1:1179880/150‑1 (MQ=255)
                          cAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCattat                                                                                                                          >  1:1150137/1‑150 (MQ=255)
                           aaCGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCattatt                                                                                                                         <  1:1103447/150‑1 (MQ=255)
                               tgatgaAGCGTTTTATGGGGATATTGCTGTTTGCATTGGGGATGGAAGTTATCGGTACTGGTATTATGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCAATGCTCCGTTTACTTCGTCATTAtttttc                                                                                                                     <  2:1147979/150‑2 (MQ=255)
                               tGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTa                                                                                                                     >  1:1631858/1‑150 (MQ=255)
                                    aCGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTg                                                                                                                <  1:1095272/150‑1 (MQ=255)
                                    aCGCGAATTATGGGGCTATTGGTGATGGCATTGGGGATTGAATTTATCGTTACTGGTTTTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGTAAGGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTg                                                                                                                <  1:1087165/150‑1 (MQ=255)
                                                gggCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGt                                                                                                    >  1:1297093/1‑150 (MQ=255)
                                                gggCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGt                                                                                                    >  1:117718/1‑150 (MQ=255)
                                                 ggCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGtt                                                                                                   >  1:1701368/1‑150 (MQ=255)
                                                    tATTGCTGATGGCATTTGTGATTGAATTTATAGTAACTTGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATc                                                                                                <  2:1625411/150‑1 (MQ=255)
                                                        gCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaa                                                                                            >  2:1645805/1‑150 (MQ=255)
                                                        gCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaa                                                                                            >  2:364562/1‑150 (MQ=255)
                                                         ctgctgGCATTGGGGATTGCAGTTATAGTTTCTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTTATTCCTTACAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGATCCAGAGCAGTTATcaaaat                                                                                           >  2:290571/1‑149 (MQ=255)
                                                         cTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaag                                                                                           >  2:119221/1‑150 (MQ=255)
                                                            aTGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaa                                                                                        <  2:479705/150‑1 (MQ=255)
                                                            aTGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaa                                                                                        >  1:642288/1‑150 (MQ=255)
                                                               gCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATcaaaagcaaaag                                                                                     >  2:876000/1‑150 (MQ=255)
                                                                cATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCTTCATTATTTTTAATTTGTTCCAGCGCAGTTATAAAAAGCATAAgg                                                                                    >  1:581159/1‑150 (MQ=255)
                                                                cATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAgg                                                                                    <  2:1319517/150‑1 (MQ=255)
                                                                 aTTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGa                                                                                   >  2:468918/1‑150 (MQ=255)
                                                                       taTTGAATTTATCGTTACTGGTATTAAGGGGATTGTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAACCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGt                                                                             <  2:1038979/149‑1 (MQ=255)
                                                                          tGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTaaa                                                                          >  2:1692960/1‑150 (MQ=255)
                                                                            aaTTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTaaaaa                                                                        >  1:1533344/1‑150 (MQ=255)
                                                                               ttATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAatat                                                                     >  1:142641/1‑150 (MQ=255)
                                                                               ttATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAatat                                                                     >  1:753369/1‑150 (MQ=255)
                                                                                tATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAtt                                                                    >  2:1305302/1‑150 (MQ=255)
                                                                                 aTCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttc                                                                   <  2:855456/150‑1 (MQ=255)
                                                                                    gTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATAttcttc                                                                >  2:1564729/1‑150 (MQ=255)
                                                                                       aCTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCa                                                             <  1:1408022/150‑1 (MQ=255)
                                                                                        cTGGTAATAAGGGGATTTTCCCCGGCCTGCATAATTAATACCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCaa                                                            >  1:1614112/1‑150 (MQ=255)
                                                                                          ggTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAt                                                          >  1:600837/1‑150 (MQ=255)
                                                                                          ggTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAt                                                          >  2:872736/1‑150 (MQ=255)
                                                                                          ggTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAt                                                          <  2:142641/150‑1 (MQ=255)
                                                                                           gTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAtt                                                         <  2:1537614/150‑1 (MQ=255)
                                                                                           gTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAtt                                                         >  1:1153228/1‑150 (MQ=255)
                                                                                             aTTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTAc                                                       >  2:299870/1‑150 (MQ=255)
                                                                                             aTTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTAc                                                       >  1:776426/1‑150 (MQ=255)
                                                                                              ttAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACa                                                      <  1:1125444/150‑1 (MQ=255)
                                                                                               tAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACag                                                     <  1:876000/150‑1 (MQ=255)
                                                                                                aaGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTTTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagt                                                    >  1:1084570/1‑150 (MQ=255)
                                                                                                  ggggATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAGGGAATAGGTAAAAATATTCTTCTCAAATTACagtta                                                  >  2:1284483/1‑150 (MQ=255)
                                                                                                  ggggATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagtta                                                  <  1:365822/150‑1 (MQ=255)
                                                                                                   gggATTATCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTCCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTCTCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagttag                                                 <  1:268789/150‑1 (MQ=255)
                                                                                                    ggATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagttagt                                                <  2:1150137/150‑1 (MQ=255)
                                                                                                     gATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACagttagtt                                               >  2:1016396/1‑150 (MQ=255)
                                                                                                       ttttCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTtat                                             >  1:1348874/1‑150 (MQ=255)
                                                                                                         ttCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAATTTACAGTTAGTTATaa                                           >  1:1584795/1‑150 (MQ=255)
                                                                                                         ttCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATaa                                           <  1:667629/150‑1 (MQ=255)
                                                                                                          tCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAg                                          <  2:741311/150‑1 (MQ=255)
                                                                                                          tCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAg                                          <  1:468918/150‑1 (MQ=255)
                                                                                                           ccccGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAgg                                         >  2:1282832/1‑150 (MQ=255)
                                                                                                           ccccGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAgg                                         >  1:761374/1‑150 (MQ=255)
                                                                                                                gCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATTCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGAtttg                                    <  1:916805/150‑2 (MQ=255)
                                                                                                                 ccTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTcc                                   <  1:1564729/150‑1 (MQ=255)
                                                                                                                 ccTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTcc                                   <  1:1069966/150‑1 (MQ=255)
                                                                                                                  cTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCt                                  <  1:1305302/150‑1 (MQ=255)
                                                                                                                   tggttaattaaTTCCTTTCAAATGAAACGGAGCTGCCATGCTACGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAACGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCtt                                 <  1:311899/147‑1 (MQ=255)
                                                                                                                    gCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTa                                >  2:661564/1‑150 (MQ=255)
                                                                                                                      ttaattaaTTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGATTACTTAGTAATTATTTTTAATTAGTACCCGCGCAGTAATCAAAAGAAAAAGGAATAGGTATAAATATTATTCTCAAATAACAGTTACTTATAAGGATTTCGTTaaa                              >  1:336742/1‑149 (MQ=255)
                                                                                                                           taattCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCtt                         >  2:1044713/1‑150 (MQ=255)
                                                                                                                                ccTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTctcct                    >  2:401407/1‑150 (MQ=255)
                                                                                                                                 cTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTAGTTTGTTCCGGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAAATAGAGTTAGTTATAAGGATTTCCTTAACTGCTTctcctg                   >  1:319852/1‑149 (MQ=255)
                                                                                                                                  tttCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCa                  <  1:119221/150‑1 (MQ=255)
                                                                                                                                  tttCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCa                  <  1:363131/150‑1 (MQ=255)
                                                                                                                                   ttCAAATGAAACGGAGCTGCCATGCTACGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCATTTATCAAAAGCAAAATGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCAc                 <  1:772646/150‑1 (MQ=255)
                                                                                                                                      aaaTGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcat              >  2:1437232/1‑150 (MQ=255)
                                                                                                                                      aaaTGAAACGGAGCTGCCATGCTCCGTATACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGTATTTCCTTAACTGCTTCTCCTCACcat              >  1:1423482/1‑150 (MQ=255)
                                                                                                                                        agtaaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatca            >  1:389994/4‑150 (MQ=255)
                                                                                                                                        agtaaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatca            >  1:1462364/4‑150 (MQ=255)
                                                                                                                                         tGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACcatcat           >  1:394159/1‑150 (MQ=255)
                                                                                                                                          taaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATg          >  2:508949/2‑150 (MQ=255)
                                                                                                                                           aaaCTGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACTGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGt         <  1:1173834/150‑1 (MQ=255)
                                                                                                                                           aaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGt         >  1:329450/1‑150 (MQ=255)
                                                                                                                                           aaaCGGAGCATGGCAGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGt         >  1:886268/1‑150 (MQ=255)
                                                                                                                                           aaaCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGt         <  1:857083/150‑1 (MQ=255)
                                                                                                                                           aaaCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGt         <  2:117718/150‑1 (MQ=255)
                                                                                                                                            aaCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGtt        >  2:621323/1‑150 (MQ=255)
                                                                                                                                            aaCGGAGCTGCCATGCTCCGTTTACTTAGTCATTATTTTTACTTTGTGCCCGAGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTATTATCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACAATAATGtt        >  2:1221444/1‑150 (MQ=255)
                                                                                                                                             aCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTa       <  1:1692960/150‑1 (MQ=255)
                                                                                                                                             aCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTa       <  1:915427/150‑1 (MQ=255)
                                                                                                                                             aCGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTATGTTCCCGCGAAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCACACcatcattata       >  2:295447/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTACGGGTTTTACGCCAGACCGGCATCAACGTGATTACGCGTATTATGGGGCTATTGCTGATGGCATTGGGGATTGAATTTATCGTTACTGGTATTAAGGGGATTTTCCCCGGCCTGCTTAATTAATTCCTTTCAAATGAAACGGAGCTGCCATGCTCCGTTTACTTCGTCATTATTTTTACTTTGTTCCCGCGCAGTTATCAAAAGCAAAAGGAATAGGTAAAAATATTCTTCTCAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTC  >  NC_000913/1299120‑1299415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: