Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,446,357:1 +T 8.6% intergenic (+151/+21) ydbL → / ← feaR DUF1318 family protein/transcriptional activator for tynA and feaB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,446,3571.T 8.6% 330.5 / 28.9 93intergenic (+151/+21)ydbL/feaRDUF1318 family protein/transcriptional activator for tynA and feaB
Reads supporting (aligned to +/- strand):  ref base . (38/47);  new base T (6/2);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

AGCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGC  >  NC_000913/1446208‑1446506
                                                                                                                                                      |                                                                                                                                                     
aGCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCa                                                                                                                                                        <  2:133224/150‑1 (MQ=255)
 gCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑g                                                                                                                                                      >  1:434156/1‑150 (MQ=255)
 gCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑g                                                                                                                                                      <  2:779069/150‑1 (MQ=255)
  cAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑Gt                                                                                                                                                     >  2:508867/1‑150 (MQ=255)
   aaaaCCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTa                                                                                                                                                    <  1:759590/150‑1 (MQ=255)
     aaCCGCAATATCGGGATATCGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACa                                                                                                                                                  <  1:660968/150‑1 (MQ=255)
     aaCCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGAGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCG‑TTACa                                                                                                                                                  >  1:1324677/1‑150 (MQ=255)
      aCCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACAt                                                                                                                                                 <  1:741802/150‑1 (MQ=255)
       ccGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATg                                                                                                                                                <  1:1301489/150‑1 (MQ=255)
        cGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGc                                                                                                                                               <  2:1213243/150‑1 (MQ=255)
         tcAATATCTTTATAACGCTACATGATAAGTCGATTTATCTATATTCCTTAAAAAATGTTAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGATTTAACTAATTGGCGTTGCA‑GTACATGCa                                                                                                                                              <  1:225932/149‑1 (MQ=255)
          cAATATCGGGATAACGCTACAGGAGAAGGCTATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCaa                                                                                                                                             <  1:275944/150‑1 (MQ=255)
            atatCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACg                                                                                                                                           <  1:1617030/150‑1 (MQ=255)
             tatCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGc                                                                                                                                          <  1:182516/150‑1 (MQ=255)
              atCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACGcc                                                                                                                                         <  2:1421104/150‑1 (MQ=255)
               tCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCa                                                                                                                                        <  2:1003418/150‑1 (MQ=255)
                cGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCaa                                                                                                                                       >  2:800060/1‑150 (MQ=255)
                cGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCaa                                                                                                                                       <  1:1718468/150‑1 (MQ=255)
                cGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCaa                                                                                                                                       >  1:657667/1‑150 (MQ=255)
                cGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAAAGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCaa                                                                                                                                       <  1:81559/150‑1 (MQ=255)
                  ggATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAtt                                                                                                                                     >  2:144371/1‑150 (MQ=255)
                     tAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAttagt                                                                                                                                  <  1:48220/150‑1 (MQ=255)
                       aCGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCATGTAC‑TGCAACGCCAAttagtta                                                                                                                                <  1:1298751/150‑1 (MQ=255)
                           tACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCgg                                                                                                                            <  1:1104997/150‑1 (MQ=255)
                             cAGGAGAAGGCGATTGATCTATTTTTCTGAAACAAGGGGGATATTCAAAAACTCCAGTCAAATTTGCTTTTGGCCGGAAAAATGCATAGTGAAATAAATGTTTTTACTAATTGGCGTTGCA‑GTACATGCAACGCCAAtttgttttaggaa                                                                                                                          >  2:855924/1‑143 (MQ=255)
                               ggAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAAtt                                                                                                                        <  2:372689/150‑1 (MQ=255)
                               ggAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGACTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACTCCAATTAGTTAGCGGAAtt                                                                                                                        >  2:1051467/1‑150 (MQ=255)
                                    aGGCGATTGATCTATTTTCCTGAAACAAGGTGATTATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGt                                                                                                                   >  2:76231/1‑150 (MQ=255)
                                     ggCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATTCATAGTCAAATATCTGTTTTAACTAATTGGCGTGGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTc                                                                                                                  <  2:1547033/150‑1 (MQ=255)
                                        gATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAATTATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGc                                                                                                                                               >  1:176899/1‑118 (MQ=255)
                                        gATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGc                                                                                                                                               <  2:176899/118‑1 (MQ=255)
                                                 aTTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTTTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTg                                                                                                      <  2:1056345/150‑1 (MQ=255)
                                                 aTTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTg                                                                                                      >  1:188987/1‑150 (MQ=255)
                                                  ttttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTgg                                                                                                     <  2:440600/150‑1 (MQ=255)
                                                    ttCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCg                                                                                                   <  1:8723/150‑1 (MQ=255)
                                                        tGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCAt                                                                                               >  2:229224/1‑150 (MQ=255)
                                                               aGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAg                                                                                        <  2:919111/150‑1 (MQ=255)
                                                                ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGc                                                                                       >  1:1622452/1‑150 (MQ=255)
                                                                ggTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGc                                                                                       >  1:1439135/1‑150 (MQ=255)
                                                                  tGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGt                                                                                     >  1:861516/1‑150 (MQ=255)
                                                                     atatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc                                                                                  <  2:551445/150‑1 (MQ=255)
                                                                     atatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGc                                                                                  <  2:1645867/150‑1 (MQ=255)
                                                                      tatTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCt                                                                                 <  1:201540/150‑1 (MQ=255)
                                                                        ttCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTa                                                                               <  2:378990/150‑1 (MQ=255)
                                                                        ttCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTa                                                                               >  1:876479/1‑150 (MQ=255)
                                                                          cAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTaaa                                                                             >  2:1479532/1‑150 (MQ=255)
                                                                                  ccTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTg                                                                     <  1:187693/150‑1 (MQ=255)
                                                                                    tGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCATCGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCGGGCGGCATCCCAAAGCGTTGCTTAATTACCGTTGaa                                                                   >  2:870246/1‑150 (MQ=255)
                                                                                       cAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGaaaaa                                                                <  2:545178/150‑1 (MQ=255)
                                                                                        aaaTTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAAt                                                               >  2:354316/1‑150 (MQ=255)
                                                                                            tGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGAct                                                           >  1:77911/1‑150 (MQ=255)
                                                                                                ttttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg                                                       <  1:718073/150‑1 (MQ=255)
                                                                                                ttttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCGGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTgg                                                       <  1:247241/150‑1 (MQ=255)
                                                                                                  ttGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTc                                                     <  1:1310444/150‑1 (MQ=255)
                                                                                                   tGCCCTGAAAAATTCATAGTCAAATATCTTTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGAGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa                                                    <  1:902729/150‑1 (MQ=255)
                                                                                                   tGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCa                                                    <  1:508867/150‑1 (MQ=255)
                                                                                                    gCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAg                                                   >  2:863992/1‑150 (MQ=255)
                                                                                                     cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGa                                                  >  1:196486/1‑150 (MQ=255)
                                                                                                     cccTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGa                                                  >  2:1247800/1‑150 (MQ=255)
                                                                                                       cTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAAATAGTTAGCGTAATTTAAGTCGATACTCGCCTGGCGTCATCCAAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGaaa                                                >  1:156794/1‑150 (MQ=255)
                                                                                                         taaaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAAt                                              <  1:797304/149‑1 (MQ=255)
                                                                                                         gAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAAt                                              >  1:1435327/1‑150 (MQ=255)
                                                                                                          aaaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATc                                             <  2:1238268/150‑1 (MQ=255)
                                                                                                           aaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATcc                                            >  2:1112767/1‑150 (MQ=255)
                                                                                                           aaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATcc                                            <  2:790385/150‑1 (MQ=255)
                                                                                                           aaaaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATcc                                            <  1:229224/150‑1 (MQ=255)
                                                                                                             aaTGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATcccc                                          <  2:434156/150‑1 (MQ=255)
                                                                                                                 cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATg                                      >  1:1261189/1‑150 (MQ=255)
                                                                                                                 cATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATg                                      >  1:470221/1‑150 (MQ=255)
                                                                                                                     gTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAg                                  <  1:1112767/150‑1 (MQ=255)
                                                                                                                         aaTATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGa                              <  2:1622452/150‑1 (MQ=255)
                                                                                                                              cTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcct                         >  2:55334/1‑150 (MQ=255)
                                                                                                                                gTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcctgc                       >  2:190392/1‑150 (MQ=255)
                                                                                                                                 ttttAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcctgcc                      <  2:339616/150‑1 (MQ=255)
                                                                                                                                 ttttAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcctgcc                      >  1:1371389/1‑150 (MQ=255)
                                                                                                                                 ttttAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGAtgcctgcc                      <  1:248863/150‑1 (MQ=255)
                                                                                                                                    tAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGt                   <  2:308422/150‑1 (MQ=255)
                                                                                                                                     aaCTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGtt                  >  2:631231/1‑150 (MQ=255)
                                                                                                                                     aaCTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGtt                  <  2:77911/150‑1 (MQ=255)
                                                                                                                                       cTAATTGGCGTTGCATGTAC‑TGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGtttt                >  2:353447/1‑150 (MQ=255)
                                                                                                                                       cTAATTGGCGTTGCATGTA‑ATGCAACGCCAATTAGTTAGCGGATTTTACGTAGATACTCGCATGGCGTCATCCCAAAGCTTTGCTTAAATACCGTTTAAAAATGACTAAGGTCATAAATTCACCAATGAAAGACTATGAATGCCAtttat                >  1:150335/1‑148 (MQ=255)
                                                                                                                                       cTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGtttt                <  1:1247800/150‑1 (MQ=255)
                                                                                                                                       cTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGtttt                >  1:1131002/1‑150 (MQ=255)
                                                                                                                                       cTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGtttt                >  1:469572/1‑150 (MQ=255)
                                                                                                                                        tAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGttttt               <  2:188987/150‑1 (MQ=255)
                                                                                                                                         aaTTGGCGTTGCATGTAC‑TGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtc              >  2:14178/1‑150 (MQ=255)
                                                                                                                                          aTTGGCGTTGCATGTAC‑TGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtca             >  1:715607/1‑150 (MQ=255)
                                                                                                                                            tGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtc              <  2:396626/147‑1 (MQ=255)
                                                                                                                                            tGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtc              >  1:396626/1‑147 (MQ=255)
                                                                                                                                             ggCGTTGCATGTAC‑TGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTtcatca          >  2:830090/1‑150 (MQ=255)
                                                                                                                                             ggCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTACCAGTTTTtcatca          >  1:393296/1‑150 (MQ=255)
                                                                                                                                              gCGTTGCATGTAC‑TGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTcatcat         >  2:658544/1‑150 (MQ=255)
                                                                                                                                               cGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGAGACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGCCTCTGGTCAGAACATCCCCAATGACAGCCGATGCCTGCCAGTTTTTCatcatc        >  1:278916/1‑150 (MQ=255)
                                                                                                                                                  tGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCTATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATTAAAGCCGATGCCTGCCAGTTTTTCATCATCTGc     <  1:217483/150‑1 (MQ=255)
                                                                                                                                                   gCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCg    >  1:960409/1‑150 (MQ=255)
                                                                                                                                                    cA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCgg   >  2:991545/1‑150 (MQ=255)
                                                                                                                                                     a‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGc  >  1:960058/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
AGCAAAACCGCAATATCGGGATAACGCTACAGGAGAAGGCGATTGATCTATTTTCCTGAAACAAGGTGAATATTCAAAAACTCCTGTCAAATTGCCTTTTGCCCTGAAAAATGCATAGTCAAATATCTGTTTTAACTAATTGGCGTTGCA‑GTACATGCAACGCCAATTAGTTAGCGGAATTTACGTCGATACTCGCCTGGCGTCATCCCAAAGCGTTGCTTAAATACCGTTGAAAAATGACTCTGGTCAGAAAATCCCCAATGAAAGCCGATGCCTGCCAGTTTTTCATCATCTGCGGC  >  NC_000913/1446208‑1446506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑