Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,512,728 C→A 8.8% intergenic (‑22/‑314) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,512,7280CA 8.8% 288.0 / 13.2 107intergenic (‑22/‑314)mntH/nupCmanganese/divalent cation transporter/nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base C (50/47);  new base A (6/4);  total (56/51)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-01

Only 100 of 112 total aligned reads displayed.

TGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACA  >  NC_000913/2512579‑2512877
                                                                                                                                                     |                                                                                                                                                     
tGAATATTGGGCGCAAAGGTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGcc                                                                                                                                                       <  2:289522/150‑1 (MQ=255)
  aaTATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCtt                                                                                                                                                     <  2:554616/150‑1 (MQ=255)
  aaTATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCtt                                                                                                                                                     >  2:1030046/1‑150 (MQ=255)
   atatTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCttt                                                                                                                                                    >  1:675567/1‑150 (MQ=255)
    tatTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTg                                                                                                                                                   >  1:767005/1‑150 (MQ=255)
     atTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGc                                                                                                                                                  <  1:411318/150‑1 (MQ=255)
        ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAt                                                                                                                                               >  2:415909/1‑150 (MQ=255)
        ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTATGCTAt                                                                                                                                               >  1:825497/1‑150 (MQ=255)
           cGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGtt                                                                                                                                            >  1:1518317/1‑150 (MQ=255)
             cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                          <  2:1583289/150‑2 (MQ=255)
             cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                          <  2:1268287/150‑2 (MQ=255)
             cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTGCGTCATCTTGTGACTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                          <  2:1648358/150‑2 (MQ=255)
              aaaGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCa                                                                                                                                         <  2:1518317/150‑1 (MQ=255)
                    aCCGGGATCGATATAACCAATCGCCGGAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTAt                                                                                                                                   <  2:190627/150‑1 (MQ=255)
                    aCCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTAGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTAt                                                                                                                                   >  2:1010833/1‑150 (MQ=255)
                       gggATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGcc                                                                                                                                <  1:415909/150‑1 (MQ=255)
                          aTCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCaaa                                                                                                                             <  1:1132616/150‑1 (MQ=255)
                            cGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACg                                                                                                                           <  2:1266564/150‑1 (MQ=255)
                             gATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCCTCTTGTGCCTCTATAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACga                                                                                                                          >  1:108487/1‑150 (MQ=255)
                              atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                         >  2:128342/1‑150 (MQ=255)
                                ataACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGAGCCGCCCCTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGACTCTAAAACATAGACATAGCTATGTTTCATGCTATGCCAAACGAGaa                                                                                                                       >  1:723386/1‑150 (MQ=255)
                                 taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                      >  2:1700491/1‑150 (MQ=255)
                                 taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                      <  1:440762/150‑1 (MQ=255)
                                 taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                      >  2:673014/1‑150 (MQ=255)
                                   aCCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGa                                                                                                                    >  1:1646211/1‑150 (MQ=255)
                                      aaTCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTa                                                                                                                 <  2:818377/150‑1 (MQ=255)
                                        tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATc                                                                                                               <  1:1030046/150‑1 (MQ=255)
                                        tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATc                                                                                                               <  1:1292352/150‑1 (MQ=255)
                                          gccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaa                                                                                                             >  1:135894/1‑150 (MQ=255)
                                             gcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAAtt                                                                                                          >  2:795712/1‑150 (MQ=255)
                                             gcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAAtt                                                                                                          <  2:1378809/150‑1 (MQ=255)
                                               aaTGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCa                                                                                                        >  2:130151/1‑150 (MQ=255)
                                                 tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                      >  2:116467/1‑150 (MQ=255)
                                                 tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCAGTCCTCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGACTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                      <  1:227719/150‑1 (MQ=255)
                                                   caCGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACACTCAACGCGATAGTTCGTCATCTGGTGCCTCTACAACCTAGCCTTTGCTATGTGTGATGCTCTGCCGATCGAGAATGATTATCAAAGTCATTTa                                                                                                    >  1:71459/2‑150 (MQ=255)
                                                      gCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAAt                                                                                                 >  1:1323305/1‑150 (MQ=255)
                                                       cAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCACTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATg                                                                                                >  1:1008224/1‑150 (MQ=255)
                                                           tCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTTTGTTTCATGCTATGCCAACCGAGAAGGATTATCAATTTCATTTTATTGGAtt                                                                                            >  1:1137933/1‑150 (MQ=255)
                                                            accATTAATGCGAGCCTCTTCTTTCGCGCCGACAGTCCGCTTCTACTATCAACGCGAAAGTTCGTCATCTTGTGCCTCTAAAACATAGCATATGCTTTGTATCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtg                                                                                           <  2:44251/149‑1 (MQ=255)
                                                             ccATTAATGCGAGCCTCATGTTGCGCGCAGACCGTCCGCTGCTACTCTCAACGCGATTGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGACAAACTAGAATGATTATCAAATTCATTTAAATGGATtgt                                                                                          <  2:244323/150‑1 (MQ=255)
                                                             ccATTAATGAGAGCCTCATCTTGCGCGCCTCCCGTCCGCTGATACTCTCAACGCGATAGTTAGTCATCTTGTTCCTCTAAAAGATATCCTATGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATtgt                                                                                          <  1:325092/150‑1 (MQ=255)
                                                                ttAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggt                                                                                       <  1:750727/150‑1 (MQ=255)
                                                                 tAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggtg                                                                                      <  2:1606129/150‑1 (MQ=255)
                                                                 tAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGACAATGATTATCAAATTCATTTAAATGGATTgtggtg                                                                                      <  2:675567/150‑1 (MQ=255)
                                                                  aaTGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGa                                                                                     <  1:130151/150‑1 (MQ=255)
                                                                   aTGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAt                                                                                    <  2:825497/150‑1 (MQ=255)
                                                                   aTGCGAGCCTCATCTTGCGCACCGCCCGTCCGCTGCTACTCTCAACGCTATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAt                                                                                    <  2:355302/150‑1 (MQ=255)
                                                                    tGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAtt                                                                                   >  1:374765/1‑150 (MQ=255)
                                                                    tGCGAGCCTCATCTTGCGCGCCGCCCGGCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGGGAtt                                                                                   <  2:245373/150‑1 (MQ=255)
                                                                     gCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAttt                                                                                  <  2:1028727/150‑1 (MQ=255)
                                                                        tgCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTGAACGAGATTGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTtctc                                                                               <  1:953455/149‑1 (MQ=255)
                                                                         gCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTctct                                                                              >  2:1280191/1‑150 (MQ=255)
                                                                            tCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGGTCGGCATCTTGTGCCTCTAAAACATAGCCTGGGCGATGTTGCATGCGATGCCAACCGAGATTGATTATCAAATTCATGTAAATGGATTCTGGGGATTTCTCTCAt                                                                           >  1:120609/1‑150 (MQ=255)
                                                                             cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                          >  1:1263134/1‑150 (MQ=255)
                                                                             cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                          <  2:358776/150‑1 (MQ=255)
                                                                             cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                          <  2:1299828/150‑1 (MQ=255)
                                                                                 ttGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAcc                                                                      <  1:504543/150‑1 (MQ=255)
                                                                                      cgccgcCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAt                                                                 <  1:673014/150‑1 (MQ=255)
                                                                                          gccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                             <  1:1567265/150‑1 (MQ=255)
                                                                                          gccCGTCCGCTGCTACCCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                             >  2:1452990/1‑150 (MQ=255)
                                                                                           cccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGa                                                            >  1:624167/1‑150 (MQ=255)
                                                                                           cccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGa                                                            >  2:1315813/1‑150 (MQ=255)
                                                                                            ccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATATCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTTGTGATTTCTCTGATAGACCAGGATTATGaa                                                           >  1:707675/1‑149 (MQ=255)
                                                                                              gTCCGCTGCTACTCTCAACGCGATAGTTCGTCATATTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTa                                                         <  1:854454/150‑1 (MQ=255)
                                                                                               gccGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTAc                                                        <  2:168485/149‑1 (MQ=255)
                                                                                                ccGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACg                                                       <  1:945959/150‑1 (MQ=255)
                                                                                                    tgctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGaaaa                                                   <  1:1117286/150‑1 (MQ=255)
                                                                                                      ctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGa                                                 >  2:43203/1‑150 (MQ=255)
                                                                                                       tACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAt                                                >  2:1501978/1‑150 (MQ=255)
                                                                                                          tctcAACGCGCTTGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGCTTATGACTACGAAAAGATtga                                             <  2:1707803/150‑1 (MQ=255)
                                                                                                                    aTAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAt                                                <  2:72504/137‑1 (MQ=255)
                                                                                                                    aTAGTTCGTCATCTTGTGCATCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAt                                                >  1:72504/1‑137 (MQ=255)
                                                                                                                     tAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATg                                  <  2:767005/150‑1 (MQ=255)
                                                                                                                      aGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgt                                 <  2:135894/150‑1 (MQ=255)
                                                                                                                       gTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgtt                                >  1:715688/1‑150 (MQ=255)
                                                                                                                          cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                             >  1:514215/1‑150 (MQ=255)
                                                                                                                          cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATAACTACGAAAAGATTGATGACTTGAATgttatg                             >  2:1652340/1‑150 (MQ=255)
                                                                                                                           gTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatgg                            >  2:1194370/1‑150 (MQ=255)
                                                                                                                            tCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggt                           <  1:1700491/150‑1 (MQ=255)
                                                                                                                            tCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggt                           <  1:116467/150‑1 (MQ=255)
                                                                                                                              aTCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggtta                         >  2:1338328/1‑150 (MQ=255)
                                                                                                                                  tgtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGtt                     >  2:919652/1‑150 (MQ=255)
                                                                                                                                  tgtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGtt                     <  1:1315813/150‑1 (MQ=255)
                                                                                                                                    tgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTa                   >  1:528868/1‑150 (MQ=255)
                                                                                                                                     gCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTaa                  >  2:1621365/1‑150 (MQ=255)
                                                                                                                                     gCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTaa                  >  1:786170/1‑150 (MQ=255)
                                                                                                                                      ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGATAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                 >  1:1067405/1‑150 (MQ=255)
                                                                                                                                      ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                 >  1:484681/1‑150 (MQ=255)
                                                                                                                                      ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                 <  2:374765/150‑1 (MQ=255)
                                                                                                                                      ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                 >  2:1372500/1‑150 (MQ=255)
                                                                                                                                        tctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTAATTTAAATGGATTGTTGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGt               <  1:795712/150‑1 (MQ=255)
                                                                                                                                         atgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt              >  1:1257606/4‑150 (MQ=255)
                                                                                                                                         atgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt              >  2:1457374/4‑150 (MQ=255)
                                                                                                                                          tgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAATAGATTGATGACTTGATTGTTATGGTTATGTTTAATGTTa             >  1:103020/3‑150 (MQ=255)
                                                                                                                                          tgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTa             >  1:1128181/3‑150 (MQ=255)
                                                                                                                                          aaaaaCATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTa             <  2:1634145/149‑1 (MQ=255)
                                                                                                                                            aaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGc           >  2:1693487/1‑150 (MQ=255)
                                                                                                                                             aaCATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGca          >  2:1332822/1‑150 (MQ=255)
                                                                                                                                                    ccTTTGCTATGTTTCATGCTATGCCAAACGAGATTGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGCATTATGACTACGAAAAGATAGATGACTTGAATGTTATGTTTATGTTTAATGTTAGCACATTTa  >  1:143683/1‑149 (MQ=255)
                                                                                                                                                     cTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACa  >  1:429721/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACA  >  NC_000913/2512579‑2512877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: