Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,512,729 T→A 10.2% intergenic (‑23/‑313) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,512,7290TA10.2% 266.1 / 14.9 105intergenic (‑23/‑313)mntH/nupCmanganese/divalent cation transporter/nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base T (47/47);  new base A (8/3);  total (55/50)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 111 total aligned reads displayed.

AATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACAT  >  NC_000913/2512581‑2512878
                                                                                                                                                    |                                                                                                                                                     
aaTATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCtt                                                                                                                                                      >  2:1292352/1‑150 (MQ=255)
aaTATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCtt                                                                                                                                                      >  2:1030046/1‑150 (MQ=255)
 atatTGGTCGCAAAGTTACCGGGATCTATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATACTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATATCCttt                                                                                                                                                     <  1:566537/150‑1 (MQ=255)
  tatTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTg                                                                                                                                                    >  1:767005/1‑150 (MQ=255)
  tatTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTg                                                                                                                                                    <  1:135237/150‑1 (MQ=255)
   atTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGc                                                                                                                                                   <  1:411318/150‑1 (MQ=255)
      ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAt                                                                                                                                                >  2:415909/1‑150 (MQ=255)
      ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTATGCTAt                                                                                                                                                >  1:825497/1‑150 (MQ=255)
      ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCACCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAt                                                                                                                                                >  1:355302/1‑150 (MQ=255)
         cGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGtt                                                                                                                                             >  1:1518317/1‑150 (MQ=255)
          gCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGttt                                                                                                                                            <  2:625555/150‑1 (MQ=255)
           cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                           <  2:1583289/150‑2 (MQ=255)
           cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                           <  2:1268287/150‑2 (MQ=255)
           cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTGCGTCATCTTGTGACTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                           <  2:1648358/150‑2 (MQ=255)
            aaaGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCa                                                                                                                                          <  2:1518317/150‑1 (MQ=255)
                  aCCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTAGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTAt                                                                                                                                    >  2:1010833/1‑150 (MQ=255)
                        aTCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCaaa                                                                                                                              <  1:1132616/150‑1 (MQ=255)
                          cGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCTTCATCTAGTGCCTCTAAAACATAGCCATTGATATCTTTCATGCTATGCCAACCg                                                                                                                            >  1:114862/1‑150 (MQ=255)
                          cGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACg                                                                                                                            <  2:1266564/150‑1 (MQ=255)
                           gATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCCTCTTGTGCCTCTATAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACga                                                                                                                           >  1:108487/1‑150 (MQ=255)
                            atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                          >  2:128342/1‑150 (MQ=255)
                              ataACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGAGCCGCCCCTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGACTCTAAAACATAGACATAGCTATGTTTCATGCTATGCCAAACGAGaa                                                                                                                        >  1:723386/1‑150 (MQ=255)
                               taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                       >  2:1700491/1‑150 (MQ=255)
                               taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                       <  1:440762/150‑1 (MQ=255)
                               taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                       >  2:673014/1‑150 (MQ=255)
                                 aCCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGa                                                                                                                     >  1:1646211/1‑150 (MQ=255)
                                    aaTCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTa                                                                                                                  <  2:818377/150‑1 (MQ=255)
                                      tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATc                                                                                                                <  1:1030046/150‑1 (MQ=255)
                                      tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATc                                                                                                                <  1:1292352/150‑1 (MQ=255)
                                        gccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaa                                                                                                              >  1:135894/1‑150 (MQ=255)
                                           gcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAAtt                                                                                                           <  2:1378809/150‑1 (MQ=255)
                                           gcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAAtt                                                                                                           >  2:795712/1‑150 (MQ=255)
                                             aaTGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCa                                                                                                         >  2:130151/1‑150 (MQ=255)
                                               tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                       >  2:116467/1‑150 (MQ=255)
                                               tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCAGTCCTCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGACTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                       <  1:227719/150‑1 (MQ=255)
                                                 caCGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACACTCAACGCGATAGTTCGTCATCTGGTGCCTCTACAACCTAGCCTTTGCTATGTGTGATGCTCTGCCGATCGAGAATGATTATCAAAGTCATTTa                                                                                                     >  1:71459/2‑150 (MQ=255)
                                                    gCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAAt                                                                                                  >  1:1323305/1‑150 (MQ=255)
                                                     cAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCACTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATg                                                                                                 >  1:1008224/1‑150 (MQ=255)
                                                         tCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTTTGTTTCATGCTATGCCAACCGAGAAGGATTATCAATTTCATTTTATTGGAtt                                                                                             >  1:1137933/1‑150 (MQ=255)
                                                          accATTAATGCGAGCCTCTTCTTTCGCGCCGACAGTCCGCTTCTACTATCAACGCGAAAGTTCGTCATCTTGTGCCTCTAAAACATAGCATATGCTTTGTATCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtg                                                                                            <  2:44251/149‑1 (MQ=255)
                                                           ccATTAATGCGAGCCTCATGTTGCGCGCAGACCGTCCGCTGCTACTCTCAACGCGATTGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGACAAACTAGAATGATTATCAAATTCATTTAAATGGATtgt                                                                                           <  2:244323/150‑1 (MQ=255)
                                                           ccATTAATGAGAGCCTCATCTTGCGCGCCTCCCGTCCGCTGATACTCTCAACGCGATAGTTAGTCATCTTGTTCCTCTAAAAGATATCCTATGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATtgt                                                                                           <  1:325092/150‑1 (MQ=255)
                                                              ttAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggt                                                                                        <  1:750727/150‑1 (MQ=255)
                                                               tAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGACAATGATTATCAAATTCATTTAAATGGATTgtggtg                                                                                       <  2:675567/150‑1 (MQ=255)
                                                                aaTGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGa                                                                                      <  1:130151/150‑1 (MQ=255)
                                                                 aTGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAt                                                                                     <  2:825497/150‑1 (MQ=255)
                                                                 aTGCGAGCCTCATCTTGCGCACCGCCCGTCCGCTGCTACTCTCAACGCTATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAt                                                                                     <  2:355302/150‑1 (MQ=255)
                                                                  tGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAtt                                                                                    >  1:374765/1‑150 (MQ=255)
                                                                  tGCGAGCCTCATCTTGCGCGCCGCCCGGCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGGGAtt                                                                                    <  2:245373/150‑1 (MQ=255)
                                                                   gCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAttt                                                                                   <  2:1028727/150‑1 (MQ=255)
                                                                      tgCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTGAACGAGATTGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTtctc                                                                                <  1:953455/149‑1 (MQ=255)
                                                                       gCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTctct                                                                               >  2:1280191/1‑150 (MQ=255)
                                                                          tCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGGTCGGCATCTTGTGCCTCTAAAACATAGCCTGGGCGATGTTGCATGCGATGCCAACCGAGATTGATTATCAAATTCATGTAAATGGATTCTGGGGATTTCTCTCAt                                                                            >  1:120609/1‑150 (MQ=255)
                                                                           cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                           <  2:358776/150‑1 (MQ=255)
                                                                           cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                           <  2:1299828/150‑1 (MQ=255)
                                                                           cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                           >  1:1263134/1‑150 (MQ=255)
                                                                               ttGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAcc                                                                       <  1:504543/150‑1 (MQ=255)
                                                                                    cgccgcCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAt                                                                  <  1:673014/150‑1 (MQ=255)
                                                                                        gccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                              <  1:1567265/150‑1 (MQ=255)
                                                                                        gccCGTCCGCTGCTACCCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                              >  2:1452990/1‑150 (MQ=255)
                                                                                         cccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGa                                                             >  1:624167/1‑150 (MQ=255)
                                                                                         cccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGa                                                             >  2:1315813/1‑150 (MQ=255)
                                                                                          ccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATATCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTTGTGATTTCTCTGATAGACCAGGATTATGaa                                                            >  1:707675/1‑149 (MQ=255)
                                                                                            gTCCGCTGCTACTCTCAACGCGATAGTTCGTCATATTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTa                                                          <  1:854454/150‑1 (MQ=255)
                                                                                             gccGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTAc                                                         <  2:168485/149‑1 (MQ=255)
                                                                                              ccGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACg                                                        <  1:945959/150‑1 (MQ=255)
                                                                                                  tgctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGaaaa                                                    <  1:1117286/150‑1 (MQ=255)
                                                                                                    ctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGa                                                  >  2:43203/1‑150 (MQ=255)
                                                                                                     tACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAt                                                 >  2:1501978/1‑150 (MQ=255)
                                                                                                        tctcAACGCGCTTGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGCTTATGACTACGAAAAGATtga                                              <  2:1707803/150‑1 (MQ=255)
                                                                                                                  aTAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAt                                                 <  2:72504/137‑1 (MQ=255)
                                                                                                                  aTAGTTCGTCATCTTGTGCATCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAt                                                 >  1:72504/1‑137 (MQ=255)
                                                                                                                   tAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATg                                   <  2:767005/150‑1 (MQ=255)
                                                                                                                    aGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgt                                  <  2:135894/150‑1 (MQ=255)
                                                                                                                     gTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgtt                                 >  1:715688/1‑150 (MQ=255)
                                                                                                                        cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                              >  1:514215/1‑150 (MQ=255)
                                                                                                                        cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATAACTACGAAAAGATTGATGACTTGAATgttatg                              >  2:1652340/1‑150 (MQ=255)
                                                                                                                         gTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatgg                             <  2:1646211/150‑1 (MQ=255)
                                                                                                                          tCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggt                            <  1:1700491/150‑1 (MQ=255)
                                                                                                                              cgtgtgCCTCTAAAACATAGCCTTTGCTATGGTTGAGGCTATGCCAAACGAGAATGATTATCAAATTGATTTAAATGGAGGGTGGTGAGTTCTCTGAGAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggttatg                        >  2:273864/3‑150 (MQ=255)
                                                                                                                                tgtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGtt                      <  1:1315813/150‑1 (MQ=255)
                                                                                                                                  tgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTa                    >  1:528868/1‑150 (MQ=255)
                                                                                                                                   gCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTaa                   >  2:1621365/1‑150 (MQ=255)
                                                                                                                                   gCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTaa                   >  1:786170/1‑150 (MQ=255)
                                                                                                                                    ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGATAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                  >  1:1067405/1‑150 (MQ=255)
                                                                                                                                    ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                  <  2:374765/150‑1 (MQ=255)
                                                                                                                                    ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                  >  1:484681/1‑150 (MQ=255)
                                                                                                                                    ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                  >  2:1372500/1‑150 (MQ=255)
                                                                                                                                     gcatgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATg                 >  1:74696/6‑150 (MQ=255)
                                                                                                                                       atgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt               >  2:1457374/4‑150 (MQ=255)
                                                                                                                                       atgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt               >  1:1257606/4‑150 (MQ=255)
                                                                                                                                        tgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAATAGATTGATGACTTGATTGTTATGGTTATGTTTAATGTTa              >  1:103020/3‑150 (MQ=255)
                                                                                                                                        tgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTa              >  1:1128181/3‑150 (MQ=255)
                                                                                                                                        aaaaaCATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTa              <  2:1634145/149‑1 (MQ=255)
                                                                                                                                          aaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGc            >  2:1693487/1‑150 (MQ=255)
                                                                                                                                           aaCATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGca           >  2:1332822/1‑150 (MQ=255)
                                                                                                                                             cATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAGTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGcaca         >  2:1419866/1‑150 (MQ=255)
                                                                                                                                                   cTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACa   >  1:429721/1‑150 (MQ=255)
                                                                                                                                                   cTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACa   <  2:1008224/150‑1 (MQ=255)
                                                                                                                                                    tttGCTATGTTTCTTGCTATGCCAAACTAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACAAGTATTATGACTAAGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACAt  <  2:705836/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACAT  >  NC_000913/2512581‑2512878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: