Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,512,731 T→G 9.4% intergenic (‑25/‑311) mntH ← / → nupC manganese/divalent cation transporter/nucleoside (except guanosine) transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,512,7310TG 9.4% 289.9 / 18.2 105intergenic (‑25/‑311)mntH/nupCmanganese/divalent cation transporter/nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base T (47/47);  new base G (7/3);  total (55/50)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

Only 100 of 109 total aligned reads displayed.

ATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAA  >  NC_000913/2512582‑2512880
                                                                                                                                                     |                                                                                                                                                     
atatTGGTCGCAAAGTTACCGGGATCTATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGATACTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATATCCttt                                                                                                                                                       <  1:566537/150‑1 (MQ=255)
atatTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCttt                                                                                                                                                       >  1:675567/1‑150 (MQ=255)
 tatTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTg                                                                                                                                                      <  1:135237/150‑1 (MQ=255)
  atTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGc                                                                                                                                                     <  1:411318/150‑1 (MQ=255)
     ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAt                                                                                                                                                  >  2:415909/1‑150 (MQ=255)
     ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTATGCTAt                                                                                                                                                  >  1:825497/1‑150 (MQ=255)
     ggTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCACCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTAt                                                                                                                                                  >  1:355302/1‑150 (MQ=255)
        cGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGtt                                                                                                                                               >  1:1518317/1‑150 (MQ=255)
         gCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGttt                                                                                                                                              <  2:625555/150‑1 (MQ=255)
          cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                             <  2:1268287/150‑2 (MQ=255)
          cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                             <  2:1583289/150‑2 (MQ=255)
          cAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTGCGTCATCTTGTGACTCTAAAACATAGCAAAGGCTATGtttt                                                                                                                                             <  2:1648358/150‑2 (MQ=255)
           aaaGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCa                                                                                                                                            <  2:1518317/150‑1 (MQ=255)
                 aCCGGGATCGATATAACCAATCGCCGGAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTAt                                                                                                                                      <  2:190627/150‑1 (MQ=255)
                 aCCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTAGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTAt                                                                                                                                      >  2:1010833/1‑150 (MQ=255)
                    gggATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGcc                                                                                                                                   <  1:415909/150‑1 (MQ=255)
                       aTCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCaaa                                                                                                                                <  1:1132616/150‑1 (MQ=255)
                         cGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCTTCATCTAGTGCCTCTAAAACATAGCCATTGATATCTTTCATGCTATGCCAACCg                                                                                                                              >  1:114862/1‑150 (MQ=255)
                          gATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCCTCTTGTGCCTCTATAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACga                                                                                                                             >  1:108487/1‑150 (MQ=255)
                           atatAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACgag                                                                                                                            >  2:128342/1‑150 (MQ=255)
                             ataACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGAGCCGCCCCTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGACTCTAAAACATAGACATAGCTATGTTTCATGCTATGCCAAACGAGaa                                                                                                                          >  1:723386/1‑150 (MQ=255)
                              taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                         <  1:440762/150‑1 (MQ=255)
                              taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                         >  2:673014/1‑150 (MQ=255)
                              taACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAAt                                                                                                                         >  2:1700491/1‑150 (MQ=255)
                                aCCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGa                                                                                                                       >  1:1646211/1‑150 (MQ=255)
                                   aaTCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTa                                                                                                                    <  2:818377/150‑1 (MQ=255)
                                     tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATc                                                                                                                  <  1:1030046/150‑1 (MQ=255)
                                     tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATc                                                                                                                  <  1:1292352/150‑1 (MQ=255)
                                       gccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCaa                                                                                                                >  1:135894/1‑150 (MQ=255)
                                          gcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAAtt                                                                                                             >  2:795712/1‑150 (MQ=255)
                                          gcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAAtt                                                                                                             <  2:1378809/150‑1 (MQ=255)
                                            aaTGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCa                                                                                                           >  2:130151/1‑150 (MQ=255)
                                              tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                         >  2:116467/1‑150 (MQ=255)
                                              tGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCAGTCCTCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGACTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCAtt                                                                                                         <  1:227719/150‑1 (MQ=255)
                                                caCGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACACTCAACGCGATAGTTCGTCATCTGGTGCCTCTACAACCTAGCCTTTGCTATGTGTGATGCTCTGCCGATCGAGAATGATTATCAAAGTCATTTa                                                                                                       >  1:71459/2‑150 (MQ=255)
                                                   gCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAAt                                                                                                    >  1:1323305/1‑150 (MQ=255)
                                                    cAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCACTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATg                                                                                                   >  1:1008224/1‑150 (MQ=255)
                                                        tCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTTTGTTTCATGCTATGCCAACCGAGAAGGATTATCAATTTCATTTTATTGGAtt                                                                                               >  1:1137933/1‑150 (MQ=255)
                                                         accATTAATGCGAGCCTCTTCTTTCGCGCCGACAGTCCGCTTCTACTATCAACGCGAAAGTTCGTCATCTTGTGCCTCTAAAACATAGCATATGCTTTGTATCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATtg                                                                                              <  2:44251/149‑1 (MQ=255)
                                                          ccATTAATGCGAGCCTCATGTTGCGCGCAGACCGTCCGCTGCTACTCTCAACGCGATTGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGACAAACTAGAATGATTATCAAATTCATTTAAATGGATtgt                                                                                             <  2:244323/150‑1 (MQ=255)
                                                          ccATTAATGAGAGCCTCATCTTGCGCGCCTCCCGTCCGCTGATACTCTCAACGCGATAGTTAGTCATCTTGTTCCTCTAAAAGATATCCTATGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATtgt                                                                                             <  1:325092/150‑1 (MQ=255)
                                                             ttAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggt                                                                                          <  1:750727/150‑1 (MQ=255)
                                                              tAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggtg                                                                                         <  2:1606129/150‑1 (MQ=255)
                                                              tAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGACAATGATTATCAAATTCATTTAAATGGATTgtggtg                                                                                         <  2:675567/150‑1 (MQ=255)
                                                               aaTGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGa                                                                                        <  1:130151/150‑1 (MQ=255)
                                                                aTGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAt                                                                                       <  2:825497/150‑1 (MQ=255)
                                                                aTGCGAGCCTCATCTTGCGCACCGCCCGTCCGCTGCTACTCTCAACGCTATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAt                                                                                       <  2:355302/150‑1 (MQ=255)
                                                                 tGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAtt                                                                                      >  1:374765/1‑150 (MQ=255)
                                                                 tGCGAGCCTCATCTTGCGCGCCGCCCGGCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGGGAtt                                                                                      <  2:245373/150‑1 (MQ=255)
                                                                  gCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGAttt                                                                                     <  2:1028727/150‑1 (MQ=255)
                                                                     tgCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTGAACGAGATTGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTtctc                                                                                  <  1:953455/149‑1 (MQ=255)
                                                                      gCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTctct                                                                                 >  2:1280191/1‑150 (MQ=255)
                                                                         tCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGGTCGGCATCTTGTGCCTCTAAAACATAGCCTGGGCGATGTTGCATGCGATGCCAACCGAGATTGATTATCAAATTCATGTAAATGGATTCTGGGGATTTCTCTCAt                                                                              >  1:120609/1‑150 (MQ=255)
                                                                          cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                             <  2:358776/150‑1 (MQ=255)
                                                                          cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                             <  2:1299828/150‑1 (MQ=255)
                                                                          cATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATa                                                                             >  1:1263134/1‑150 (MQ=255)
                                                                              ttGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAcc                                                                         <  1:504543/150‑1 (MQ=255)
                                                                                   cgccgcCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAt                                                                    <  1:673014/150‑1 (MQ=255)
                                                                                       gccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                                <  1:1567265/150‑1 (MQ=255)
                                                                                       gccCGTCCGCTGCTACCCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATg                                                                >  2:1452990/1‑150 (MQ=255)
                                                                                        cccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGa                                                               >  2:1315813/1‑150 (MQ=255)
                                                                                        cccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGa                                                               >  1:624167/1‑150 (MQ=255)
                                                                                         ccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATATCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTTGTGATTTCTCTGATAGACCAGGATTATGaa                                                              >  1:707675/1‑149 (MQ=255)
                                                                                           gTCCGCTGCTACTCTCAACGCGATAGTTCGTCATATTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTa                                                            <  1:854454/150‑1 (MQ=255)
                                                                                             ccGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACg                                                          <  1:945959/150‑1 (MQ=255)
                                                                                                 tgctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGaaaa                                                      <  1:1117286/150‑1 (MQ=255)
                                                                                                   ctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGa                                                    >  2:43203/1‑150 (MQ=255)
                                                                                                    tACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAt                                                   >  2:1501978/1‑150 (MQ=255)
                                                                                                       tctcAACGCGCTTGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGCTTATGACTACGAAAAGATtga                                                <  2:1707803/150‑1 (MQ=255)
                                                                                                                 aTAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAt                                                   <  2:72504/137‑1 (MQ=255)
                                                                                                                 aTAGTTCGTCATCTTGTGCATCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGAt                                                   >  1:72504/1‑137 (MQ=255)
                                                                                                                  tAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATg                                     <  2:767005/150‑1 (MQ=255)
                                                                                                                   aGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgt                                    <  2:135894/150‑1 (MQ=255)
                                                                                                                    gTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgtt                                   >  1:715688/1‑150 (MQ=255)
                                                                                                                       cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                                >  1:514215/1‑150 (MQ=255)
                                                                                                                       cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATAACTACGAAAAGATTGATGACTTGAATgttatg                                >  2:1652340/1‑150 (MQ=255)
                                                                                                                        gTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatgg                               >  2:1194370/1‑150 (MQ=255)
                                                                                                                        gTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatgg                               <  2:1646211/150‑1 (MQ=255)
                                                                                                                         tCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggt                              <  1:116467/150‑1 (MQ=255)
                                                                                                                           aTCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggtta                            >  2:1338328/1‑150 (MQ=255)
                                                                                                                             cgtgtgCCTCTAAAACATAGCCTTTGCTATGGTTGAGGCTATGCCAAACGAGAATGATTATCAAATTGATTTAAATGGAGGGTGGTGAGTTCTCTGAGAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatggttatg                          >  2:273864/3‑150 (MQ=255)
                                                                                                                               tgtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGtt                        <  1:1315813/150‑1 (MQ=255)
                                                                                                                                  gCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTaa                     >  1:786170/1‑150 (MQ=255)
                                                                                                                                  gCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTaa                     >  2:1621365/1‑150 (MQ=255)
                                                                                                                                   ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGATAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                    >  1:1067405/1‑150 (MQ=255)
                                                                                                                                   ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                    <  2:374765/150‑1 (MQ=255)
                                                                                                                                   ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                    >  1:484681/1‑150 (MQ=255)
                                                                                                                                   ccTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAAt                    >  2:1372500/1‑150 (MQ=255)
                                                                                                                                    gcatgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATg                   >  1:74696/6‑150 (MQ=255)
                                                                                                                                     tctAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTAATTTAAATGGATTGTTGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGt                  <  1:795712/150‑1 (MQ=255)
                                                                                                                                      atgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGtt                 >  2:1457374/4‑150 (MQ=255)
                                                                                                                                       tgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAATAGATTGATGACTTGATTGTTATGGTTATGTTTAATGTTa                >  1:103020/3‑150 (MQ=255)
                                                                                                                                       tgaaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTa                >  1:1128181/3‑150 (MQ=255)
                                                                                                                                       aaaaaCATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTa                <  2:1634145/149‑1 (MQ=255)
                                                                                                                                         aaaCATAGCAAAGGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGc              >  2:1693487/1‑150 (MQ=255)
                                                                                                                                          aaCATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGca             >  2:1332822/1‑150 (MQ=255)
                                                                                                                                                 ccTTTGCTATGTTTCATGCTATGCCAAACGAGATTGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGCATTATGACTACGAAAAGATAGATGACTTGAATGTTATGTTTATGTTTAATGTTAGCACATTTaa      >  1:143683/1‑149 (MQ=255)
                                                                                                                                                  cTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACa     <  2:1008224/150‑1 (MQ=255)
                                                                                                                                                  cTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACa     >  1:429721/1‑150 (MQ=255)
                                                                                                                                                   tttGCTATGTTTCTTGCTATGCCAAACTAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACAAGTATTATGACTAAGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACAt    <  2:705836/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATATTGGTCGCAAAGTTACCGGGATCGATATAACCAATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACATTTACATAA  >  NC_000913/2512582‑2512880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: