Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,519,225 T→A 5.2% intergenic (+20/+32) yfeD → / ← gltX DUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,519,2250TA 5.2% 325.3 / 4.2 111intergenic (+20/+32)yfeD/gltXDUF1323 family putative DNA‑binding protein/glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (54/51);  new base A (2/4);  total (56/55)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 116 total aligned reads displayed.

GGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCAC  >  NC_000913/2519076‑2519372
                                                                                                                                                     |                                                                                                                                                   
ggCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCTCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCTATATTGACGAATCAGCATAAATAAAAAACGGCAGGAtatt                                                                                                                                                     <  1:834858/150‑1 (MQ=255)
   tattccaCAAATCACCAACCCGAAAGATAATATGACATACATTGACCCGGAAAAAGCCGCGCAATTTTTGACCCGTGAAGGAATTCCCAATTACCACGCCCGTCTCGATATTGACGAATCACCAAAACTAAACACCGGCAGGAAATTAtc                                                                                                                                                  <  2:620745/144‑1 (MQ=255)
   gTATCGACAAATCTTCTACCGTAAACTAAATATGTCATACATTGAGCTTGATAAAGGCGCGCAATTTTTGTCACGTGAAGTAATTCCCAATTTCCACGCCCGTCTCTATATTGACGAATCAGCATAATTAAAACACGGCAGGATATTAtc                                                                                                                                                  <  2:1354938/150‑1 (MQ=255)
   gTATCGACAAATCATCAACGCGATAGATAAAATGACAGACATTGAGCAGGAAAAAGTCGCGGAATTTTTGTCACGTGAAGGAATTCGCAATTTACTCTCCCGACTCGATATTGACGAATCAGAAAAAATAAAAAACGGAAGGAtattata                                                                                                                                                  >  2:862416/1‑149 (MQ=255)
    tATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtct                                                                                                                                                 >  1:1181429/1‑150 (MQ=255)
        gACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                                                                             >  1:999528/1‑150 (MQ=255)
        caaaaaTCATCAACGCGATTCATAATATGACAGACATTGAGCAGTAAAAAGTCGCGCAATTTTTGTCACGTGAAGTAATTCGAAATTTCCTCGCCCGTCTCTATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTg                                                                                                                                             <  2:1104152/147‑1 (MQ=255)
         aCAAATCATCACCGCGATAGATAATTTGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTTTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGc                                                                                                                                            <  1:1098232/150‑1 (MQ=255)
           aaaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCg                                                                                                                                          <  1:833709/150‑1 (MQ=255)
           aaaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCg                                                                                                                                          <  1:1528464/150‑1 (MQ=255)
           aaaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGCCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCg                                                                                                                                          <  1:1373900/150‑1 (MQ=255)
            aaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGt                                                                                                                                         <  2:1242778/150‑1 (MQ=255)
            aaTCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGt                                                                                                                                         <  1:1322158/150‑1 (MQ=255)
             atcatcAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGtt                                                                                                                                        <  1:232719/150‑1 (MQ=255)
              tcatcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGttt                                                                                                                                       <  2:125250/150‑1 (MQ=255)
               catcaACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGAAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGAGATAATATCCTGCCGtttt                                                                                                                                      <  1:1191176/150‑2 (MQ=255)
                  caACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCt                                                                                                                                   >  2:1229224/1‑150 (MQ=255)
                  caACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCt                                                                                                                                   >  1:1262776/1‑150 (MQ=255)
                  caACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCt                                                                                                                                   <  2:82090/150‑1 (MQ=255)
                     cgcgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCTTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCtttt                                                                                                                                <  1:927518/150‑1 (MQ=255)
                       cgATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAtctc                                                                                                                                                >  2:260032/1‑132 (MQ=255)
                               aataTGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGCCAGGATATTATGTCCGGCCGTTTATCTTTTTACCCGCTaa                                                                                                                      >  1:305680/1‑150 (MQ=255)
                                   tGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTAc                                                                                                                  >  2:1007686/1‑150 (MQ=255)
                                     acagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActg                                                                                                                >  1:334562/1‑150 (MQ=255)
                                      cagacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgc                                                                                                               >  2:127054/1‑150 (MQ=255)
                                       agacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgct                                                                                                              <  1:426626/150‑1 (MQ=255)
                                        gacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCGGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgctg                                                                                                             >  2:1309583/1‑150 (MQ=255)
                                        gacaTTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTActgctg                                                                                                             >  2:1563607/1‑150 (MQ=255)
                                              gAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTCTACACGCTAATTACTGCTGATTTTc                                                                                                       <  2:1348203/150‑1 (MQ=255)
                                               atcatgAAAAAGTCGCGCAATTTTTGTCACGTGAATGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATAAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                      <  2:1505145/145‑1 (MQ=255)
                                               aGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTcg                                                                                                      >  2:1686940/1‑150 (MQ=255)
                                                gCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTAAACGCTAATTACTGCTGATTTTcgc                                                                                                     >  1:1631275/1‑150 (MQ=255)
                                                  agtaaaaaGTCGCCCAATTTTTGTCACGTTAAGTAATTCGCAATTTCCTCGCCCGTCTCGATTTTGACGAATCAGCAAAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGt                                                                                                   <  1:1451116/147‑1 (MQ=255)
                                                  aGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGt                                                                                                   >  1:166140/1‑150 (MQ=255)
                                                  aGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGt                                                                                                   >  1:722350/1‑150 (MQ=255)
                                                  aGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGt                                                                                                   <  1:915182/150‑1 (MQ=255)
                                                   gtaaaaaGTCGCGCAATTTTTGTCACGTGATGGAATTCGCAATTTCCTGGCCCGTCTGGATTTTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGtt                                                                                                  <  1:716842/148‑1 (MQ=255)
                                                    taaaaaGTCGCTAAATTTTTGTAACGTGAATGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTc                                                                                                 <  1:1303457/149‑1 (MQ=255)
                                                      aaaaGTCGCGAAATTTTTGTCACGTGAACGAATTCGCAATTACCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAg                                                                                               <  2:663038/150‑1 (MQ=255)
                                                          gTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAAt                                                                                           >  1:768530/1‑150 (MQ=255)
                                                            cgcgCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaa                                                                                         <  1:1007686/150‑1 (MQ=255)
                                                            cgcgCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGAAATaa                                                                                         >  2:1522480/1‑150 (MQ=255)
                                                             gcgcAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATaaa                                                                                        >  1:524283/1‑150 (MQ=255)
                                                             gcgcAATTTTGGACACGGGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTAGCTCCTGCCGTGTATCTTTTTACACGCTAATTACTCAAGCTTTTCGCGGTCAGCAATaaa                                                                                        >  1:1379734/1‑150 (MQ=255)
                                                                       tGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCt                                                                              >  1:1281971/1‑150 (MQ=255)
                                                                       tGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCt                                                                              >  2:758919/1‑150 (MQ=255)
                                                                        gTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCtt                                                                             <  2:1347018/150‑1 (MQ=255)
                                                                          cACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttg                                                                           >  1:150603/1‑150 (MQ=255)
                                                                           aCGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgt                                                                          >  2:1001472/1‑150 (MQ=255)
                                                                            cGTTAAGTAATTCGCAATTTCCTCGCCCGTATCGATTTTGACTAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                                         <  2:1631275/150‑1 (MQ=255)
                                                                            cGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTttgtt                                                                         <  1:9088/150‑1 (MQ=255)
                                                                               gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                                      >  2:661977/1‑150 (MQ=255)
                                                                               gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                                      <  2:693874/150‑1 (MQ=255)
                                                                               gAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGAt                                                                      <  2:334562/150‑1 (MQ=255)
                                                                                aaGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATa                                                                     <  2:935728/150‑1 (MQ=255)
                                                                                aaGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATa                                                                     >  1:314249/1‑150 (MQ=255)
                                                                                  ggAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACg                                                                   >  1:123127/1‑150 (MQ=255)
                                                                                   taaTTCGCAATTTCCTCGCCCGTCTCGATATTGACTAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGc                                                                  <  2:511682/149‑1 (MQ=255)
                                                                                   gAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGc                                                                  >  1:1684526/1‑150 (MQ=255)
                                                                                     aTTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTc                                                                <  2:1181429/150‑1 (MQ=255)
                                                                                      ttCGCAATTTCCTCGCCCGTCTCGATATTGACGAATGAGCATAAATTAAAATCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACATGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCg                                                               <  2:937257/150‑1 (MQ=255)
                                                                                        cGCAATTTCCTCGCCCGTCTCGATATAGACGAATCAGCATAAATAAACAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGAt                                                             <  2:268547/150‑1 (MQ=255)
                                                                                         gCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATa                                                            <  1:784290/150‑1 (MQ=255)
                                                                                                ccTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCggg                                                     >  2:983874/1‑150 (MQ=255)
                                                                                                 cTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGt                                                    >  1:1327569/1‑150 (MQ=255)
                                                                                                   cGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCt                                                  >  2:554415/1‑150 (MQ=255)
                                                                                                    gCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCtt                                                 >  1:354195/1‑150 (MQ=255)
                                                                                                            cGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                                         <  2:768530/150‑1 (MQ=255)
                                                                                                            cGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                                         >  2:761382/1‑150 (MQ=255)
                                                                                                            cGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTg                                         >  2:886630/1‑150 (MQ=255)
                                                                                                              atatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGAAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCg                                       >  1:98643/1‑150 (MQ=255)
                                                                                                               tatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGCTACTGCGGGGCTTACCAATTGCGt                                      >  2:1227398/1‑150 (MQ=255)
                                                                                                               tatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTTCGt                                      >  2:1532577/1‑150 (MQ=255)
                                                                                                               tatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGt                                      >  1:1173064/1‑150 (MQ=255)
                                                                                                               tatTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGAGt                                      >  1:731891/1‑150 (MQ=255)
                                                                                                                atTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTg                                     <  2:1173064/150‑1 (MQ=255)
                                                                                                                 tttaagAATCAGCTTAAATAAAATACGGCAGGATATTATCTCCTCCCGTTTATCTTTTTACACGCTAATTAGTGCTGATTTTCTCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGa                                    <  1:862416/145‑1 (MQ=255)
                                                                                                                  tGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGaa                                   >  1:68142/1‑150 (MQ=255)
                                                                                                                   gACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAAc                                  >  1:1330833/1‑150 (MQ=255)
                                                                                                                         tCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAAc                            <  2:314249/150‑1 (MQ=255)
                                                                                                                           aGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACAt                          >  2:1255621/1‑150 (MQ=255)
                                                                                                                            gCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATc                         >  2:522924/1‑150 (MQ=255)
                                                                                                                            gCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATc                         >  1:178208/1‑150 (MQ=255)
                                                                                                                             cATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATcc                        <  2:150603/150‑1 (MQ=255)
                                                                                                                                   agataaaCGGCAGGAGATAATATCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCt                  >  2:979758/5‑150 (MQ=255)
                                                                                                                                    aaaaaaCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCGCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTg                 <  2:359130/150‑1 (MQ=255)
                                                                                                                                       aatctgCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGgag              <  2:984586/145‑1 (MQ=255)
                                                                                                                                         aCGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGAc            <  2:166140/150‑1 (MQ=255)
                                                                                                                                            gCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGAAATAAAATCCAGCGATTTGTTGATACGCTCGATACTGAGGGTCTTAACAATTGCGTGAACGGTAAAATCCAGTGCTGGATACTGc         >  1:1471412/1‑150 (MQ=255)
                                                                                                                                            gCAGGATATTATCTCCTGCAGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGc         <  2:622573/150‑1 (MQ=255)
                                                                                                                                             cAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGcc        >  2:59456/1‑150 (MQ=255)
                                                                                                                                              aGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGccc       <  1:491441/150‑1 (MQ=255)
                                                                                                                                               ggATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGcccc      <  1:1563607/150‑1 (MQ=255)
                                                                                                                                                gATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCg     <  2:1330833/150‑1 (MQ=255)
                                                                                                                                                gATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCg     >  2:1654628/1‑150 (MQ=255)
                                                                                                                                                 atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGGCTGCCCCGc    >  2:1676644/1‑150 (MQ=255)
                                                                                                                                                 atatTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTAAGCAATAAAATCAAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTTCCCCGc    >  2:1020837/1‑150 (MQ=255)
                                                                                                                                                  tattatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCa   >  1:1671159/1‑150 (MQ=255)
                                                                                                                                                   attatCTCGTGCCGTTTATCTTTTTACAATCTAATTACTGCTGATTTTCGCGTTGAGCAATAAATTCCATCGATTTTTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGTACGGTAACATCCAGTGCTGGAGACTGCGCCGCAc  <  2:665238/150‑1 (MQ=255)
                                                                                                                                                   attatCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCAc  >  2:932083/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GGCGTATCGACAAATCATCAACGCGATAGATAATATGACAGACATTGAGCAGGAAAAAGTCGCGCAATTTTTGTCACGTGAAGGAATTCGCAATTTCCTCGCCCGTCTCGATATTGACGAATCAGCATAAATAAAAAACGGCAGGATATTATCTCCTGCCGTTTATCTTTTTACACGCTAATTACTGCTGATTTTCGCGTTCAGCAATAAAATCCAGCGCTTTGTTGATACGCTCGATACTGCGGGTCTTACCAATTGCGTGAACGGTAACATCCAGTGCTGGAGACTGCCCCGCAC  >  NC_000913/2519076‑2519372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: