Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,523,798	T→G	7.4%	D63A (GAC→GCC)	xapB ←	xanthosine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,523,798	0	T	G	7.4%	255.4 / 7.4	91	D63A (GAC→GCC)	xapB	xanthosine transporter
Reads supporting (aligned to +/- strand):  ref base T (45/39);  new base G (5/2);  total (50/41)
Fisher's exact test for biased strand distribution p-value = 4.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAACGCAATAGTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTCCCCCAGATAAAATATTGC  >  NC_000913/2523649‑2523947                                                                                                                                                      |                                                                                                                                                      aTAACGCAATAGTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGt                                                                                                                                                       <  2:856174/150‑1 (MQ=255)    aCGCAATAGTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTg                                                                                                                                                    <  2:1021248/150‑1 (MQ=255)    aCGCAATAGTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTg                                                                                                                                                    >  2:801972/1‑150 (MQ=255)     cGCAATAGTCGGCATAAATGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGc                                                                                                                                                   >  1:29168/1‑150 (MQ=255)      gCAATAGTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCg                                                                                                                                                  <  1:1071189/150‑1 (MQ=255)      gCAATAGTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCACACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCg                                                                                                                                                  <  2:1140437/150‑1 (MQ=255)       cAATAGTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCga                                                                                                                                                 <  1:801972/150‑1 (MQ=255)         aTAGTCGGCATAAACGCCATCGCAATGACTACCATCACCCAAAACATCATATCCGGATCAGTTACGGGTGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCCCGTACTGCGCGCAGCCATTTGTCTGCgatg                                                                                                                                               >  1:450703/1‑150 (MQ=255)          tAGTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGACAAATGGCTGCgcgca                                                                                                                                              <  2:834010/150‑5 (MQ=255)            gTCGGCATAAACGCCATTGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACTGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATc                                                                                                                                            <  1:167131/150‑1 (MQ=255)            gTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATc                                                                                                                                            >  1:608828/1‑150 (MQ=255)            gTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATc                                                                                                                                            >  1:1402875/1‑150 (MQ=255)               ggCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATcccc                                                                                                                                         <  2:41586/150‑1 (MQ=255)               ggCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATcccc                                                                                                                                         <  2:419670/150‑1 (MQ=255)                gCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTCCGCCCGCACACCCCAGGTGCCACCGCATGTATGCAAGTTCTGCGCGCAGCCATTTTTCTGCGCTGATcaccc                                                                                                                                        >  1:321122/1‑149 (MQ=255)                        gCCATCGCATTGACTAACCTCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCa                                                                                                                                <  2:921354/150‑1 (MQ=255)                         ccATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTCCGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAg                                                                                                                               >  2:637630/1‑150 (MQ=255)                         ccATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAg                                                                                                                               <  1:1207797/150‑1 (MQ=255)                          cATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAgg                                                                                                                              >  2:1697016/1‑150 (MQ=255)                              gCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCata                                                                                                                          >  2:1711458/1‑150 (MQ=255)                               cATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCataa                                                                                                                         >  2:1396869/1‑150 (MQ=255)                                 ttGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCAGGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCataata                                                                                                                       <  2:775923/150‑1 (MQ=255)                                   gACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATaataat                                                                                                                     >  2:960268/1‑150 (MQ=255)                                      tAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATcgc                                                                                                                  <  1:1575405/150‑1 (MQ=255)                                      tAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATcgc                                                                                                                  <  2:1402875/150‑1 (MQ=255)                                      tAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATcgc                                                                                                                  <  1:1218760/150‑1 (MQ=255)                                       aaCATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATcgcg                                                                                                                 >  2:342910/1‑150 (MQ=255)                                       aaCATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATcgcg                                                                                                                 <  2:1321342/150‑1 (MQ=255)                                        aCATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCgcgg                                                                                                                <  2:629485/150‑1 (MQ=255)                                             aCCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACGAGGCATAATAATCGCGGCGATc                                                                                                           >  2:1594466/1‑150 (MQ=255)                                               ccAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATccc                                                                                                         >  2:1363559/1‑150 (MQ=255)                                                 aaaaCATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCtt                                                                                                       <  2:1096382/150‑1 (MQ=255)                                                   aaCATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTg                                                                                                     <  2:1255167/150‑1 (MQ=255)                                                    aCATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTgg                                                                                                    >  1:1586375/1‑150 (MQ=255)                                                    aCATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGAGCCCTTTgg                                                                                                    >  2:314098/1‑150 (MQ=255)                                                    aCATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTGCTGCGCGCAGCCATTTGGCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTgg                                                                                                    <  2:216939/150‑1 (MQ=255)                                                     catcatATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGCTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGa                                                                                                   >  1:1321670/1‑150 (MQ=255)                                                      atcatATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGaa                                                                                                  <  1:191183/150‑1 (MQ=255)                                                         atatCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTg                                                                                               <  2:970792/150‑1 (MQ=255)                                                         atatCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTg                                                                                               <  2:320866/150‑1 (MQ=255)                                                          tatCCGTAGCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACCGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGt                                                                                              <  1:87192/150‑1 (MQ=255)                                                           atCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGTTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTa                                                                                             <  2:906663/150‑1 (MQ=255)                                                              gggATCAGTTACGGATGACGCATAAAAAAGTACGCCCGCACACACCATGTGACACAGCATGTATGCACGTTCTGGGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAAc                                                                                          <  2:1686499/149‑1 (MQ=255)                                                                gATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCa                                                                                        >  2:1688478/1‑150 (MQ=255)                                                                      ttACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCCGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCaa                                                                                  >  2:1298111/1‑150 (MQ=255)                                                                      ttACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCaa                                                                                  >  2:1353708/1‑150 (MQ=255)                                                                       tACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTAAGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGAACCCTTTGGAACTGTAAACCATGCCAAc                                                                                 >  2:326590/1‑150 (MQ=255)                                                                         cGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACAt                                                                               <  1:466668/150‑1 (MQ=255)                                                                          ggctGCCGCATAAAAAAGTACGCCCGCCCACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCCTTTGTCTGCGATGATCCCCATTCTACCAGGCCTAATAATCGCGGCGATCCCTTTGCAACTGTCACCCCTGCCATCAtt                                                                              >  2:1506873/4‑150 (MQ=255)                                                                          ggATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACAtt                                                                              >  2:797588/1‑150 (MQ=255)                                                                           gATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTa                                                                             >  2:643249/1‑150 (MQ=255)                                                                            aTGCAGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAg                                                                            <  1:1178951/150‑1 (MQ=255)                                                                               ccGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAgcgc                                                                         >  1:98272/1‑150 (MQ=255)                                                                               ccGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAgcgc                                                                         >  1:444125/1‑150 (MQ=255)                                                                                 ggaTAAAAAAGTACGCCGGCACACACCAGGTGACACAGCATGTATGCACTTTCTGAGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCGTTTGGAACTGTATACCATGCCAACATTAGCGCCg                                                                       <  1:912127/148‑1 (MQ=255)                                                                                 gCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCg                                                                       <  2:98272/150‑1 (MQ=255)                                                                                   aTAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGt                                                                     >  2:1698199/1‑150 (MQ=255)                                                                                      aaaaaGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGaa                                                                  <  1:1396869/150‑1 (MQ=255)                                                                                        aacgTACGCCCGCACACACCAGGTGACACAGCTTGTAGGCACGTTCTGCGCGCAGCCATTTGTCTGCTATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAAt                                                                <  2:1511259/147‑1 (MQ=255)                                                                                        aaaGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAAt                                                                <  2:325611/150‑1 (MQ=255)                                                                                           gTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGaa                                                             >  2:1379902/1‑150 (MQ=255)                                                                                             aCGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCAATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAaga                                                           >  1:346766/1‑150 (MQ=255)                                                                                               gCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGt                                                         >  1:844205/1‑150 (MQ=255)                                                                                               acccGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGt                                                         >  1:1712278/2‑150 (MQ=255)                                                                                                 ccGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCGGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTAt                                                       <  2:1685041/150‑1 (MQ=255)                                                                                                   gCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTa                                                     >  1:14207/1‑150 (MQ=255)                                                                                                          ccAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGTCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGt                                              <  1:342910/150‑1 (MQ=255)                                                                                                          ccAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGAGATCCCTTTGGAACTGTAAACCATGCCAAAATTAGCGCCGGTGAAATGAAGAGTATTAATCATGt                                              >  1:1550385/1‑150 (MQ=255)                                                                                                             ggTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATAAGCGCCGGTGAAATGAAGAGTATTAATCATGTAag                                           >  2:342164/1‑150 (MQ=255)                                                                                                                  cacaGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGATGAGTATTAATCATGTAAGAGCCg                                      >  1:862925/1‑150 (MQ=255)                                                                                                                  cacaGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCg                                      >  2:523552/1‑150 (MQ=255)                                                                                                                   acaGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGa                                     >  1:413754/1‑150 (MQ=255)                                                                                                                    ctgtctttATTCAAGTTCTGAGCGCATCAATTTGTCTGCGATTATCCAAATTATACAAGGCATAATAATAGCGGAGATCCCTTTTGAACTGTAAACCATGCAAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAg                                    <  2:1060513/143‑1 (MQ=255)                                                                                                                    caGCATGTATGCACGTACTGCGCGCACCCATTTGTCTGCGATGATGCCCATTATAGCAGGGTAATTAATCGCGGCGATCCCTTTGGATCTGTTTTCCATGCCAACATTAGCGCCGGTGAAATGAAGCGTTTTATTCATGTAAGAGCCGAg                                    <  2:1066038/150‑1 (MQ=255)                                                                                                                     aGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAgg                                   <  2:1182061/150‑1 (MQ=255)                                                                                                                       cATGTATGCACGTTCTGCGCGCAACCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGt                                 >  2:1503399/1‑150 (MQ=255)                                                                                                                       cATGTAAGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGt                                 <  1:326590/150‑1 (MQ=255)                                                                                                                           tATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAAcc                             >  1:1027230/1‑150 (MQ=255)                                                                                                                            aTGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCGGGTGAAATGAAGAGTATTTATCATGTAAGAGCCGAGGGTATcca                            >  2:1143409/1‑150 (MQ=255)                                                                                                                               cACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAAccagcc                         <  2:29168/150‑1 (MQ=255)                                                                                                                                    gatcatcgcAGACAAATGGCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTc                    >  2:1544677/7‑150 (MQ=255)                                                                                                                                    gatcatcgcAGACAAATGGCTGCGATGATCCCCATTAAACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCTAGGGTAACCACCCAGCTc                    >  2:305643/7‑150 (MQ=255)                                                                                                                                     cTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTcc                   >  2:904914/1‑150 (MQ=255)                                                                                                                                      tGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTccc                  >  1:589512/1‑150 (MQ=255)                                                                                                                                       catcgcAGACAAATGGCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTcccc                 >  1:605042/4‑150 (MQ=255)                                                                                                                                        cgcgcAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTccccc                >  1:935336/1‑150 (MQ=255)                                                                                                                                        atcgcAGACAAATGGCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTccccc                >  2:799035/3‑150 (MQ=255)                                                                                                                                        atcgcAGACAAATGGCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCAGGTGAAATGAAGATTATTATTAATGTAAGATCCGAGGGTAACCAGCCAGCTcccac                >  1:1487278/3‑148 (MQ=255)                                                                                                                                          cgcAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTCCCCCAg              >  2:431109/1‑150 (MQ=255)                                                                                                                                          cgcAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTCCCCCAg              <  2:1027230/150‑1 (MQ=255)                                                                                                                                              gCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATATTCGCGGCGAGCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTCCCCCAGATaa          >  2:1108148/1‑150 (MQ=255)                                                                                                                                                     gTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTCCCCCAGATAAAATATTg   <  1:637630/150‑1 (MQ=255)                                                                                                                                                      tCTGCGATGATCCCCATTATACCATGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTCCCCCAGATAAAATATTGc  <  1:1711458/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      ATAACGCAATAGTCGGCATAAACGCCATCGCATTGACTAACATCACCCAAAACATCATATCCGGATCAGTTACGGATGCCGCATAAAAAAGTACGCCCGCACACACCAGGTGACACAGCATGTATGCACGTTCTGCGCGCAGCCATTTGTCTGCGATGATCCCCATTATACCAGGCATAATAATCGCGGCGATCCCTTTGGAACTGTAAACCATGCCAACATTAGCGCCGGTGAAATGAAGAGTATTAATCATGTAAGAGCCGAGGGTAACCAGCCAGCTCCCCCAGATAAAATATTGC  >  NC_000913/2523649‑2523947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑