Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,525,908 C→G 5.3% intergenic (+17/+22) yfeN → / ← yfeR putative outer membrane protein/transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,9080CG 5.3% 367.3 / 7.1 108intergenic (+17/+22)yfeN/yfeRputative outer membrane protein/transcriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base C (51/51);  new base G (3/3);  total (54/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

Only 100 of 109 total aligned reads displayed.

GGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACT  >  NC_000913/2525760‑2526057
                                                                                                                                                    |                                                                                                                                                     
ggCGCAGTTGCACTGTTGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTAACAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTTCCGGGGCt                                                                                                                                                      >  1:1215921/1‑150 (MQ=255)
ggCGCAGTTGCACTGTGGTGGAATGCAACATCACACAATACTACGGGGATTCAGAATCGATATGCGGATGACAAACTGGGCGAAGATTATTAACAGGATGCGCTAATCAACACCATCAAATTTAACTTCAGATTGATTACTGCCGGGGCt                                                                                                                                                      >  1:1215925/1‑150 (MQ=255)
    cAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCg                                                                                                                                                  <  1:594543/150‑1 (MQ=255)
      gTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGc                                                                                                                                                <  2:1374196/150‑1 (MQ=255)
        tGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAg                                                                                                                                              >  2:1111576/1‑150 (MQ=255)
        tGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAg                                                                                                                                              >  1:1593853/1‑150 (MQ=255)
         gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGt                                                                                                                                             >  1:836497/1‑150 (MQ=255)
         gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGt                                                                                                                                             <  2:189584/150‑1 (MQ=255)
         gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGt                                                                                                                                             <  2:53637/150‑1 (MQ=255)
          cACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGTa                                                                                                                                            <  1:1245584/150‑1 (MQ=255)
            cTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGAGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATg                                                                                                                                          >  1:757878/1‑150 (MQ=255)
             tgtgTTTGAATGCAACATCACACATTACTACGGTTATTCAGTATCGATATGCGGATGACAAACTGTTCTAAGATTATTACAAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGTaatc                                                                                                                                         <  1:695204/150‑4 (MQ=255)
               tggtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAAt                                                                                                                                       <  1:207861/150‑1 (MQ=255)
                ggtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAAtt                                                                                                                                      >  2:501520/1‑150 (MQ=255)
                 gtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTc                                                                                                                                     <  2:1678032/150‑1 (MQ=255)
                    gAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCAct                                                                                                                                  <  2:1575766/150‑1 (MQ=255)
                        gCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtat                                                                                                                              >  1:780707/1‑150 (MQ=255)
                        gCAACATCACACATTACTACCGGGATTCAGTATCGATAAGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATAAAAATTAACTTCTGATTGATTACTTCCGGGGCTCCCGGCAGTATGAATTAAATCtaa                                                                                                                              >  2:724610/1‑149 (MQ=255)
                         cAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtata                                                                                                                             >  1:95332/1‑150 (MQ=255)
                           aCATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATc                                                                                                                           <  1:1716530/150‑1 (MQ=255)
                            cATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCt                                                                                                                          <  1:983942/150‑1 (MQ=255)
                            aaTCACACATTACTACGGTGATTCAGTATCTATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCt                                                                                                                          <  2:129038/149‑1 (MQ=255)
                             aTCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTg                                                                                                                         >  2:924921/1‑150 (MQ=255)
                               cacacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGAt                                                                                                                       >  1:1221964/1‑150 (MQ=255)
                                acacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCTGGCAGTATGAATTCACTCTATATCttcta                                                                                                                      >  2:684042/1‑146 (MQ=255)
                                acacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATa                                                                                                                      >  2:605606/1‑150 (MQ=255)
                                   caTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATTAATTCACTCTATATCTGATACaa                                                                                                                   >  1:634371/1‑150 (MQ=255)
                                      tactacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACgg                                                                                                                <  1:1529491/150‑1 (MQ=255)
                                      tactacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACgg                                                                                                                <  1:787117/150‑1 (MQ=255)
                                        ctacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGAt                                                                                                              >  1:586144/1‑150 (MQ=255)
                                          acGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATcc                                                                                                            <  2:1456434/150‑1 (MQ=255)
                                           cGGGGATTCAGTATCGATATGCGGATGTCAAACTGGGCGAAGATTTATACCACGATGCGATAATCTACTCCATCAATTTTAACTTCTGATTGATTGCTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACATCGGATccc                                                                                                           <  2:241698/150‑1 (MQ=255)
                                            ggggATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATcccc                                                                                                          <  2:925574/150‑1 (MQ=255)
                                              ggATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCTATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCtt                                                                                                        <  1:856030/150‑1 (MQ=255)
                                              ggATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCtt                                                                                                        <  2:289752/150‑1 (MQ=255)
                                                aTTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                      <  2:1130537/150‑1 (MQ=255)
                                                  tCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATGCAACGGATCCCCTTCCCg                                                                                                    >  2:423385/1‑150 (MQ=255)
                                                   cAGTATCAATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGc                                                                                                   >  2:331944/1‑150 (MQ=255)
                                                   cAGAATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCAGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGc                                                                                                   >  2:1152021/1‑150 (MQ=255)
                                                    aGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                  >  2:1214688/1‑150 (MQ=255)
                                                     gTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGccc                                                                                                 <  1:417456/150‑1 (MQ=255)
                                                      tATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                                <  1:501520/150‑1 (MQ=255)
                                                       aTCTATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAg                                                                                               <  1:390566/150‑1 (MQ=255)
                                                       aTCGATATGCGGATGACAAACTGGGAGAAGATTATTACCAGGATGCGATAATCTACTCCATCAAATATAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAg                                                                                               <  2:255671/150‑1 (MQ=255)
                                                            tatGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                          <  2:783481/150‑1 (MQ=255)
                                                            tatGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                          >  1:1652558/1‑150 (MQ=255)
                                                                 ggATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcgc                                                                                     >  2:1597816/1‑150 (MQ=255)
                                                                 ggATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcgc                                                                                     >  2:1401411/1‑150 (MQ=255)
                                                                   aTGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAt                                                                                   <  1:331944/150‑1 (MQ=255)
                                                                          aCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                            <  2:1690682/150‑1 (MQ=255)
                                                                               gCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACGCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtctc                                                                       >  2:657597/1‑150 (MQ=255)
                                                                                cGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCg                                                                      >  2:470626/1‑150 (MQ=255)
                                                                                cGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCg                                                                      >  2:1634830/1‑150 (MQ=255)
                                                                                  aaGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGTTTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTa                                                                    <  2:609323/150‑1 (MQ=255)
                                                                                    gATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                                  >  2:1492539/1‑150 (MQ=255)
                                                                                     aTTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCTGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAATAGCGCATTGCCATGGTCTCGTACCa                                                                 >  1:1084578/1‑150 (MQ=255)
                                                                                         ttACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTc                                                             <  2:505462/150‑1 (MQ=255)
                                                                                          tACCAGGATGCGATAATCTACTCCATCAAATTTACCTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTcc                                                            <  1:727490/150‑1 (MQ=255)
                                                                                           aCCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTccc                                                           >  1:442803/1‑150 (MQ=255)
                                                                                            ccAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCa                                                          >  2:71832/1‑150 (MQ=255)
                                                                                            ccAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCa                                                          >  1:995047/1‑150 (MQ=255)
                                                                                              aGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCATCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATa                                                        <  1:1152021/150‑1 (MQ=255)
                                                                                               ggATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCAGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaa                                                       <  1:1325888/150‑1 (MQ=255)
                                                                                                 aTGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAg                                                     <  2:1380948/150‑1 (MQ=255)
                                                                                                 aTGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAg                                                     >  2:144711/1‑150 (MQ=255)
                                                                                                      aTAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCGCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCa                                                <  2:149142/150‑1 (MQ=255)
                                                                                                         aTCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcg                                             >  2:1625999/1‑150 (MQ=255)
                                                                                                           ctactaCATCAATTTTAAATTCTGATTGATTACTGCCGGGTCTACCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggc                                           <  1:724610/150‑1 (MQ=255)
                                                                                                              cTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                        <  2:787743/150‑1 (MQ=255)
                                                                                                              cTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                        <  1:1492539/150‑1 (MQ=255)
                                                                                                               tCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                                       <  1:71832/150‑1 (MQ=255)
                                                                                                                ccATCAAATTTAACTTCTGATTGATTTCTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACAGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                                      >  2:1311710/1‑150 (MQ=255)
                                                                                                                 cATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGCTCTCGTACCACGTCCCAAAAAGCTTCAGCGGCggagga                                     >  1:1104700/1‑150 (MQ=255)
                                                                                                                   tCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaaa                                   >  1:478154/1‑150 (MQ=255)
                                                                                                                    aaaaTTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATTAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAg                                  <  1:1570682/149‑1 (MQ=255)
                                                                                                                     aaaTTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGt                                 >  2:1383457/1‑150 (MQ=255)
                                                                                                                       aTTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa                               <  2:1593853/150‑1 (MQ=255)
                                                                                                                         ttAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATGCAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                             <  1:423385/150‑1 (MQ=255)
                                                                                                                          tAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACg                            <  1:470626/150‑1 (MQ=255)
                                                                                                                           aaCTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                           >  2:859093/1‑150 (MQ=255)
                                                                                                                            aCTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt                          <  1:924921/150‑1 (MQ=255)
                                                                                                                             cTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGtt                         >  1:521027/1‑150 (MQ=255)
                                                                                                                               tCTGTTTGTTTACTGCCGGGGCTCCCGGCAGTATGTATTCACTCTATATGTGATACTACGGATCCCCTTCCCGCCAAGAAATTATCGCATTTCCCTGGTCTCTTACCACGTCCCATAAAGCTTCTGCGGCTGTGGAAAGTGAACTGttct                       <  2:1215925/150‑1 (MQ=255)
                                                                                                                               tCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTTGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGtgct                       <  2:1215921/150‑1 (MQ=255)
                                                                                                                                  gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTcc                    <  1:1111576/150‑1 (MQ=255)
                                                                                                                                   attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt                   <  1:1625999/150‑1 (MQ=255)
                                                                                                                                   attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCACTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGGACCACGTCCAATAAAGCATCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt                   >  1:956560/1‑150 (MQ=255)
                                                                                                                                     tgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCCGCCATTAGCGCATTGCCCTGGGCTCGTACCCCGTCCCAGAACGCTTCAGCGGCGGTGGAAAGGGCACGGTTCTTCCTgc                 >  1:174759/1‑150 (MQ=255)
                                                                                                                                     tgattACTGCCGGGGCTCCCGGCAGTATGAATTCAATCTATATCTGATAAAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCGTGGTCTCGTACCACGACCCATATAGCTTCAGCGGCGGTGGAAAGTGAAAGGTTCTTCCTgc                 >  1:189526/1‑150 (MQ=255)
                                                                                                                                      gattACTGCCGTGGCTCCCGGCAGTATGAATTCACTCTTTATCTGATACAACGGATCCCCTTCCCGCCCATCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgct                <  2:757878/150‑2 (MQ=255)
                                                                                                                                      gattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg                >  2:600151/1‑150 (MQ=255)
                                                                                                                                       catACTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcgc               >  1:806199/3‑150 (MQ=255)
                                                                                                                                        atACTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa              >  1:1282533/2‑150 (MQ=255)
                                                                                                                                           cTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAccc           >  2:513518/1‑149 (MQ=255)
                                                                                                                                           cTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa           >  2:649069/1‑150 (MQ=255)
                                                                                                                                             gCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGc         >  2:834383/1‑150 (MQ=255)
                                                                                                                                              ccGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCa        <  1:1419452/150‑1 (MQ=255)
                                                                                                                                               cGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCCTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCAt       <  1:1214688/150‑1 (MQ=255)
                                                                                                                                                ggggCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATc      >  1:1649539/1‑150 (MQ=255)
                                                                                                                                                    cTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACt  <  2:836497/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACT  >  NC_000913/2525760‑2526057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: