Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,549,609 G→A 6.3% intergenic (+203/+37) yfeW → / ← yfeX penicillin binding protein PBP4B; weak DD‑carboxypeptidase activity/porphyrinogen oxidase, cytoplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,549,6090GA 6.3% 400.5 / 9.0 127intergenic (+203/+37)yfeW/yfeXpenicillin binding protein PBP4B; weak DD‑carboxypeptidase activity/porphyrinogen oxidase, cytoplasmic
Reads supporting (aligned to +/- strand):  ref base G (56/62);  new base A (2/6);  total (59/68)
Fisher's exact test for biased strand distribution p-value = 2.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

Only 100 of 129 total aligned reads displayed.

AAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCA  >  NC_000913/2549460‑2549758
                                                                                                                                                     |                                                                                                                                                     
aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATg                                                                                                                                                       >  1:52313/1‑150 (MQ=255)
      gCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTg                                                                                                                                                 >  2:100752/1‑150 (MQ=255)
         tGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCt                                                                                                                                              >  1:175150/1‑150 (MQ=255)
           aTGCGCTACGCTTATCCGGCCTACGTGGATCCCACAATATTTTGTATTAGCACGTTTTCGTAGGCCTGATAAGGCGTTCACTCCGCATCCGGCGTGTACAACGCGCACTTGGTCTAAAATCTGAGGCCAGTCAGTAATGTTACTGCCTgg                                                                                                                                            <  2:1605076/150‑1 (MQ=38)
           aTGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTgg                                                                                                                                            <  2:1057107/150‑1 (MQ=255)
           aTGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTACTGACTgg                                                                                                                                            <  2:1083548/150‑1 (MQ=255)
            tGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTACTGACTGGc                                                                                                                                           <  2:1055394/150‑1 (MQ=255)
             gcgcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTACTGACTGGcc                                                                                                                                          <  1:1654132/150‑1 (MQ=255)
              cgcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCt                                                                                                                                         <  1:429652/150‑1 (MQ=255)
               gcTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCtt                                                                                                                                        >  1:684444/1‑150 (MQ=255)
                cTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGGCAGTAACATTACTGACTGGCCtca                                                                                                                                       <  2:230981/150‑3 (MQ=255)
                 tACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTGGGTCAACTAACAGAGCCCAGTCAGTAATTTTACTGCCTGTACTTTa                                                                                                                                      >  2:534438/1‑150 (MQ=25)
                 gtagcgcaTCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTa                                                                                                                                      <  1:216083/143‑1 (MQ=255)
                   cGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTATGCCGTATAAGGCGTTCACGCCGCATCCGGCGTTTACAACGCTAACTTTGTAAACAATCTTAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAAt                                                                                                                                    <  2:943050/150‑1 (MQ=37)
                   cGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAAt                                                                                                                                    <  1:63632/150‑1 (MQ=255)
                     cTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCa                                                                                                                                  <  2:925548/150‑1 (MQ=255)
                         tCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGa                                                                                                                              >  1:46061/1‑150 (MQ=255)
                           aGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAAt                                                                                                                            >  2:10323/1‑150 (MQ=255)
                            ggCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATc                                                                                                                           <  1:1587994/150‑1 (MQ=255)
                              ccTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAg                                                                                                                         <  2:293557/150‑1 (MQ=255)
                              ccTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAg                                                                                                                         >  1:1574284/1‑150 (MQ=255)
                               cTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGCTACTGCCTGGCCTTTAATCAATGAATCAGa                                                                                                                        <  1:1120230/150‑1 (MQ=255)
                                    tGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGc                                                                                                                   >  2:1039557/1‑150 (MQ=255)
                                      gAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCTGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCtt                                                                                                                 <  1:759897/150‑1 (MQ=255)
                                      gAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCtt                                                                                                                 <  1:947502/150‑1 (MQ=255)
                                           cacaATATATTGAATTAGCACGATTTCGAAGGCCGGATAAGGCGTTCACGCCGCATCCGGCTTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGATACTGCCTGGCATTTAATCAAAGAATCAGAACCGATTaaatc                                                                                                            >  1:159397/1‑146 (MQ=25)
                                             caATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAgcgc                                                                                                          <  2:907391/150‑1 (MQ=255)
                                               atatatTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCa                                                                                                        >  2:1395273/1‑150 (MQ=255)
                                                      gAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACtt                                                                                                 >  1:1177225/1‑150 (MQ=255)
                                                      gAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACtt                                                                                                 <  2:1624730/150‑1 (MQ=255)
                                                       aaTTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACTCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTg                                                                                                <  2:1713173/150‑1 (MQ=255)
                                                          tAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTcc                                                                                             <  1:644171/150‑1 (MQ=255)
                                                           aGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCTCTTGGTCAACAATCTGAGGCCAGTCACTAATGTTACTGCCTGGCCTTTAATCAAAGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCa                                                                                            >  2:284999/1‑150 (MQ=255)
                                                           aGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTATAATCAATGAATCAGAAACGCTTACCGCGCCATCAACTTGTCCa                                                                                            >  2:152868/1‑150 (MQ=255)
                                                            gCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCTCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAg                                                                                           <  1:296549/150‑1 (MQ=255)
                                                             cACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGc                                                                                          >  1:995964/1‑150 (MQ=255)
                                                             cACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCAGCAACTTGTCCAGc                                                                                          <  2:722408/150‑1 (MQ=255)
                                                              aCGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCg                                                                                         <  2:7931/150‑1 (MQ=255)
                                                                 aTTTCGTAGGCCTGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACg                                                                                      <  1:1475476/150‑1 (MQ=255)
                                                                 aTTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACg                                                                                      <  2:43546/150‑1 (MQ=255)
                                                                 aTTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACg                                                                                      >  2:69874/1‑150 (MQ=255)
                                                                   ttCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGCAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGt                                                                                    >  2:247574/1‑150 (MQ=255)
                                                                    tCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTg                                                                                   >  1:1645931/1‑150 (MQ=255)
                                                                     cGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGGGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGc                                                                                  <  2:831339/150‑1 (MQ=255)
                                                                     cGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGc                                                                                  >  2:1584959/1‑150 (MQ=255)
                                                                             ggATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAaataat                                                                          >  2:1495232/1‑150 (MQ=255)
                                                                               aTAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAg                                                                        <  1:135662/150‑1 (MQ=255)
                                                                               aTAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAg                                                                        <  1:69874/150‑1 (MQ=255)
                                                                                 aaGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAgcc                                                                      <  1:1035444/150‑1 (MQ=255)
                                                                                  aGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAgccg                                                                     >  1:997162/1‑150 (MQ=255)
                                                                                   ggCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGCCGGTGCGAAATAATAgccgc                                                                    >  2:45032/1‑150 (MQ=255)
                                                                                   ggCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAgccgc                                                                    >  2:74942/1‑150 (MQ=255)
                                                                                   ggCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAgccgc                                                                    >  2:387989/1‑150 (MQ=255)
                                                                                   ggCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAgccgc                                                                    <  1:451223/150‑1 (MQ=255)
                                                                                     cGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGccgccg                                                                  <  2:684444/150‑1 (MQ=255)
                                                                                      gTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCgg                                                                 <  2:885504/150‑1 (MQ=255)
                                                                                           cgccggATGCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTAcc                                                            >  2:480301/1‑150 (MQ=255)
                                                                                            gccgcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCg                                                           >  2:1159498/1‑150 (MQ=255)
                                                                                             ccgcATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCgg                                                          >  1:462811/1‑150 (MQ=255)
                                                                                                cATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGttt                                                       <  2:1087731/150‑1 (MQ=255)
                                                                                                  tCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCTAAATAATAGCCGCCGGTTACCGGTTTgg                                                     <  1:1392285/150‑1 (MQ=255)
                                                                                                  tCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTgg                                                     >  1:1254500/1‑150 (MQ=255)
                                                                                                  tCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTgg                                                     <  1:1039557/150‑1 (MQ=255)
                                                                                                  tCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTgg                                                     <  1:776473/150‑1 (MQ=255)
                                                                                                    cGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGACACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTg                                                   >  2:695145/1‑150 (MQ=255)
                                                                                                     ggCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGa                                                  <  2:122322/150‑1 (MQ=255)
                                                                                                        gtgtACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAAc                                               <  1:187990/150‑1 (MQ=255)
                                                                                                        gtgtACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAAc                                               <  1:1125074/150‑1 (MQ=255)
                                                                                                        gtgtACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAAc                                               <  2:175150/150‑1 (MQ=255)
                                                                                                         tgtACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACg                                              >  2:1387773/1‑150 (MQ=255)
                                                                                                         tgtACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACg                                              <  2:1651543/150‑1 (MQ=255)
                                                                                                           tACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCa                                            <  1:788910/150‑1 (MQ=255)
                                                                                                             cAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAAc                                          <  2:995964/150‑1 (MQ=255)
                                                                                                                cgcgCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAACCGCTTACAGCGCCATCAACTTGTCCAGCGCCGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATc                                       >  2:154888/1‑150 (MQ=255)
                                                                                                                  cgcACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGc                                     <  1:1007803/150‑1 (MQ=255)
                                                                                                                  cgcACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGc                                     >  1:702464/1‑150 (MQ=255)
                                                                                                                      cTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCa                                 <  2:141133/150‑1 (MQ=255)
                                                                                                                      cTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCa                                 <  1:358259/150‑1 (MQ=255)
                                                                                                                          gTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGTATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCt                             >  2:1511349/1‑150 (MQ=255)
                                                                                                                             aacaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTAc                          >  1:1650419/1‑150 (MQ=255)
                                                                                                                              acaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTAcc                         >  1:613031/1‑150 (MQ=255)
                                                                                                                              acaaTCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTAcc                         >  2:650549/1‑150 (MQ=255)
                                                                                                                                  tCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCg                     <  2:52313/150‑1 (MQ=255)
                                                                                                                                   cTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCgg                    <  2:991353/150‑1 (MQ=255)
                                                                                                                                      aGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACAGGTTTGGTGAAACGCCACATCGCATCACGCTTACCATCGGTAt                 >  2:31203/1‑150 (MQ=255)
                                                                                                                                       ggCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGATACGCAACATCGCATCACGCTTACCATCGGTTTc                >  2:350481/1‑150 (MQ=255)
                                                                                                                                        gCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCg               <  1:247927/150‑1 (MQ=255)
                                                                                                                                        gCCAGGCAGTAACATTACTGACTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCg               >  1:1421550/1‑150 (MQ=255)
                                                                                                                                          cagtcagtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGcc             >  2:277386/1‑150 (MQ=255)
                                                                                                                                             tcagtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCaaa          <  2:552597/150‑1 (MQ=255)
                                                                                                                                              cagtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAAc         >  1:82067/1‑150 (MQ=255)
                                                                                                                                              cagtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAAc         >  2:728882/1‑150 (MQ=255)
                                                                                                                                               agtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACa        <  1:1584959/150‑1 (MQ=255)
                                                                                                                                               agtAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACa        <  1:1419838/150‑1 (MQ=255)
                                                                                                                                                  aaTGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGc                                     <  1:796357/118‑1 (MQ=255)
                                                                                                                                                  aaTGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGc                                     >  2:796357/1‑118 (MQ=255)
                                                                                                                                                  aaTGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGc     >  1:1275502/1‑150 (MQ=255)
                                                                                                                                                    tGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTc   <  2:82067/150‑1 (MQ=255)
                                                                                                                                                    tGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTc   <  1:100752/150‑1 (MQ=255)
                                                                                                                                                     gTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTca  <  1:973109/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGAGCCCACAATATATTGAATTAGCACGATTTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCGTGTACAACGCGCACTTGGTCAACAATCTGAGGCCAGTCAGTAATGTTACTGCCTGGCCTTTAATCAATGAATCAGAAACGCTTACAGCGCCATCAACTTGTCCAGCGACGGTGCGAAATAATAGCCGCCGGTTACCGGTTTGGTGAAACGCAACATCGCATCACGCTTACCATCGGTATCGCCAAACATGCTCA  >  NC_000913/2549460‑2549758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: