Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,560,326 A→G 6.9% T24A (ACA→GCA)  yffL → CPZ‑55 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,560,3260AG 6.9% 374.7 / 14.5 130T24A (ACA→GCA) yffLCPZ‑55 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (68/53);  new base G (4/5);  total (72/58)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.57e-01

Only 100 of 133 total aligned reads displayed.

GTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACT  >  NC_000913/2560177‑2560475
                                                                                                                                                     |                                                                                                                                                     
gTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACAAAAATAAAAGCCCCCGGAATGCTGCaa                                                                                                                                                       >  1:500124/1‑150 (MQ=255)
    ggCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATg                                                                                                                                                   <  1:1405453/150‑1 (MQ=255)
     gCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGc                                                                                                                                                  <  1:1303739/150‑1 (MQ=255)
      attctcttttTCTATAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCTTGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGcc                                                                                                                                                 <  1:1033438/146‑1 (MQ=255)
       ttttcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCg                                                                                                                                                >  2:1218315/1‑150 (MQ=255)
         ttctattTCGCTAATTATTATATTAATCCAATAAAGTGGACAAAATTATTAAATAACAAGACCTGAAATCCATGTTTGCAACGAAAGATCCATATTTCGAAAATAGGATCAACACAAAAAAAAGCCCCCACAATTCATCAACATCCCggg                                                                                                                                              <  2:263127/150‑1 (MQ=255)
          tcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCgggg                                                                                                                                             <  2:1155719/150‑1 (MQ=255)
          tcttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCgggg                                                                                                                                             <  1:329086/150‑1 (MQ=255)
           cttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggggg                                                                                                                                            <  1:272696/150‑1 (MQ=255)
           cttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggggg                                                                                                                                            <  1:943357/150‑1 (MQ=255)
           cttttTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGCATGTTGCAGCATTCCggggg                                                                                                                                            <  1:578319/150‑1 (MQ=255)
            tttttCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGc                                                                                                                                           >  2:919896/1‑150 (MQ=255)
                 cGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTAt                                                                                                                                      >  1:1459810/1‑150 (MQ=255)
                 cGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTAt                                                                                                                                      >  1:703268/1‑150 (MQ=255)
                  gCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATg                                                                                                                                     >  1:859775/1‑150 (MQ=255)
                  gCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATg                                                                                                                                     <  1:747269/150‑1 (MQ=255)
                  gCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATAACAAGAACTTAAATCCATGTATGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAAAACAAAAAAAAACCCACGGAATGCTGAAACATCACGGGGGCGGTAtt                                                                                                                                     >  1:1657129/1‑149 (MQ=255)
                   atAATTATTCAATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATga                                                                                                                                    <  2:225648/149‑1 (MQ=255)
                    tAATTATTCCATTAATCCAATTAAGTGGAATAAATTATTCAATATCAAGACCTTAAATCCATGTTTGCAACGACAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATgag                                                                                                                                   <  1:710461/150‑1 (MQ=255)
                       ttattccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTAt                                                                                                                                      <  2:1186161/144‑1 (MQ=255)
                       ttattccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGaaa                                                                                                                                <  1:1571297/150‑1 (MQ=255)
                         attccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACa                                                                                                                              <  1:1008702/150‑1 (MQ=255)
                          ttccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAATATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGAAACATGCCGGGGGCGGTATGAGAAACaa                                                                                                                             >  2:949996/1‑150 (MQ=255)
                           tccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAg                                                                                                                            >  1:979099/1‑150 (MQ=255)
                            ccattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGc                                                                                                                           <  1:1397553/150‑1 (MQ=255)
                            ccagtattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATAAAGAGTTCGAAAATAGGATCCTCACAAATAAACACCCCCGGAATCCTGCAACATGCCGGGGGCTGTATGAGAAACAAGc                                                                                                                           >  2:88763/1‑150 (MQ=255)
                             cattattccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCt                                                                                                                          >  1:612045/1‑150 (MQ=255)
                                   tccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTaaggag                                                                                                                    <  1:886528/150‑5 (MQ=255)
                                    ccaATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGgag                                                                                                                   >  2:1057810/1‑150 (MQ=255)
                                        ttAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAAtt                                                                                                               <  2:153705/150‑1 (MQ=255)
                                         tAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAAttt                                                                                                              >  2:1325656/1‑150 (MQ=255)
                                            gTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTtctc                                                                                                           >  1:547696/1‑150 (MQ=255)
                                             tttaaTAAATTATTCAATATCAAGAACTTAAATCCTTGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATTCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGATAATTTCTCa                                                                                                          <  1:539281/147‑1 (MQ=255)
                                             tGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCTACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCa                                                                                                          >  2:1546414/1‑150 (MQ=255)
                                             tGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCa                                                                                                          <  1:546039/150‑1 (MQ=255)
                                             tGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCa                                                                                                          >  1:1536468/1‑150 (MQ=255)
                                              ggAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCAt                                                                                                         >  2:1277349/1‑150 (MQ=255)
                                               gAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATg                                                                                                        >  1:798427/1‑150 (MQ=255)
                                                aaTAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGt                                                                                                       >  2:105287/1‑150 (MQ=255)
                                                     attattCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGat                                                                                                  >  2:842267/1‑150 (MQ=255)
                                                     attattCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGat                                                                                                  <  1:141569/150‑1 (MQ=255)
                                                        attCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCAAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATg                                                                                               >  2:116943/1‑150 (MQ=255)
                                                          tCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGTGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCt                                                                                             <  2:436114/150‑1 (MQ=255)
                                                           cAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATTTGCTa                                                                                            >  1:1090224/1‑150 (MQ=255)
                                                                tCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGCGAATTTCTCATGTCTGATATGCTAGCAGt                                                                                       >  1:1460920/1‑150 (MQ=255)
                                                                 cAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTa                                                                                      >  1:502642/1‑150 (MQ=255)
                                                                 cAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCAAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTa                                                                                      >  1:129199/1‑150 (MQ=255)
                                                                  aaGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAg                                                                                     <  2:265031/150‑1 (MQ=255)
                                                                   aGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGa                                                                                    <  2:1266578/150‑1 (MQ=255)
                                                                   aGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCAGGTCTGATATGCTAGCAGTAGa                                                                                    >  2:297776/1‑150 (MQ=255)
                                                                    taaCTTAAATCCATCTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACTTGCCGGTGGCGGTATGATAAACAATCTAAATGAGAATTTCTCATGTCTGATATGCTAGCAGTAGat                                                                                   <  2:817864/149‑2 (MQ=255)
                                                                     aaCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAAc                                                                                  <  2:798427/150‑1 (MQ=255)
                                                                      aCTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACa                                                                                 <  2:40290/150‑1 (MQ=255)
                                                                            aTCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaac                                                                           <  1:1218315/150‑1 (MQ=255)
                                                                              ccATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaa                                                                         >  1:539885/1‑150 (MQ=255)
                                                                                aTGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGaaacaaac                                                                       >  2:124199/1‑150 (MQ=255)
                                                                                 tGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAaacaaaca                                                                      <  1:1057810/150‑1 (MQ=255)
                                                                                        aaCGAAAGATCAAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGt                                                               <  1:1325656/150‑1 (MQ=255)
                                                                                         aCGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGtt                                                              >  1:210040/1‑150 (MQ=255)
                                                                                           taaaGATCCATAGTTCGAAAATAGGATCAACACAACTAAAAGCCCCCGTAATGCTGCAACATGCCGGGGGCTGTATGAGAAACAAGCTAAATGATAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCg                                                            <  1:1297562/149‑1 (MQ=255)
                                                                                           gAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCg                                                            <  1:1397859/150‑1 (MQ=255)
                                                                                               gATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCaa                                                        >  1:743521/1‑150 (MQ=255)
                                                                                                  ccAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAAttt                                                     <  1:425403/150‑1 (MQ=255)
                                                                                                       gTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                                >  1:1229320/1‑150 (MQ=255)
                                                                                                       gTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                                >  2:149812/1‑150 (MQ=255)
                                                                                                       gTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                                >  2:1201030/1‑150 (MQ=255)
                                                                                                       gTTCGAAAATAGGATCAACACAAATAAAAGAACCCGGAATGCTGCAACATGCAGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                                <  2:324138/150‑1 (MQ=255)
                                                                                                       gTTCAAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTaaa                                                <  1:116943/150‑1 (MQ=255)
                                                                                                         tCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTTCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACa                                              >  2:1096839/1‑150 (MQ=255)
                                                                                                         tCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACa                                              <  2:578931/150‑1 (MQ=255)
                                                                                                         tCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACa                                              >  2:1406140/1‑150 (MQ=255)
                                                                                                          cGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATACCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaa                                             >  2:714032/1‑150 (MQ=255)
                                                                                                           gAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaaa                                            >  2:1277324/1‑150 (MQ=255)
                                                                                                           gAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaaa                                            >  1:14067/1‑150 (MQ=255)
                                                                                                           gAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACaaa                                            >  2:879827/1‑150 (MQ=255)
                                                                                                               aTAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTa                                        <  2:1338358/150‑1 (MQ=255)
                                                                                                                 aGGATCAACACAAATAAAAGCCCCCGGAATGCTTCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAAc                                      <  1:1096839/150‑1 (MQ=255)
                                                                                                                 aGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAAc                                      <  2:1605930/150‑1 (MQ=255)
                                                                                                                       aaCACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTa                                >  2:211962/1‑150 (MQ=255)
                                                                                                                         cacaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACg                              >  2:1328265/1‑150 (MQ=255)
                                                                                                                         cacaAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACg                              >  2:1415503/1‑150 (MQ=255)
                                                                                                                              aTAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATa                         <  2:500124/150‑1 (MQ=255)
                                                                                                                               tAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATaa                        >  2:959311/1‑150 (MQ=255)
                                                                                                                               tAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATaa                        >  2:61506/1‑150 (MQ=255)
                                                                                                                                  ccgCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGc                     >  1:1460613/3‑150 (MQ=255)
                                                                                                                                    gCCCCCGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCAc                   >  2:896488/1‑150 (MQ=255)
                                                                                                                                       cccGGCATGTTGCAGCATTCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTAAGGAATAAAGCACCCa                >  1:990653/1‑150 (MQ=255)
                                                                                                                                       cccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCa                >  2:1626757/1‑150 (MQ=255)
                                                                                                                                        ccGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCaa               >  1:846479/1‑150 (MQ=255)
                                                                                                                                         cGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTTATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCaaa              <  2:703268/150‑1 (MQ=255)
                                                                                                                                           gAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCaaaaa            >  1:1425482/1‑150 (MQ=255)
                                                                                                                                             aTGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACg          >  1:1539191/1‑150 (MQ=255)
                                                                                                                                              tgctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATTCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGa         <  1:105287/150‑1 (MQ=255)
                                                                                                                                               gctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGaa        <  1:699218/150‑1 (MQ=255)
                                                                                                                                                ctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAag       >  2:343082/1‑150 (MQ=255)
                                                                                                                                                ctgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAag       <  1:1620144/150‑1 (MQ=255)
                                                                                                                                                ctgaAACATGCCGGTGGCGGTAAGAGAAACAAGCTAAAGGAGAATTTCTAATGTCTGATATGCACTCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAACACGAag       >  2:729728/1‑150 (MQ=255)
                                                                                                                                                 tgcAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAaga      <  2:1536468/150‑1 (MQ=255)
                                                                                                                                                    aaCATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGAc   <  1:1626757/150‑1 (MQ=255)
                                                                                                                                                     aCATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACt  >  2:724858/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTCCGGCTTTTCTTTTTCGCTAATTATTCCATTATTCCAATTAAGTGGAATAAATTATTCAATATCAAGAACTTAAATCCATGTTTGCAACGAAAGATCCAGAGTTCGAAAATAGGATCAACACAAATAAAAGCCCCCGGAATGCTGCAACATGCCGGGGGCGGTATGAGAAACAAGCTAAAGGAGAATTTCTCATGTCTGATATGCTAGCAGTAGAACAAGAAACAAACAATGATGTTCGTCAATTTTTAAACAAAATTAACGAGTTACGGAATAAAGCACCCAAAAACGAAGAGACT  >  NC_000913/2560177‑2560475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: