Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,592,990 A→C 5.5% intergenic (+28/+82) ypfN → / ← ypfH putative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,592,9900AC 5.5% 327.8 / 6.7 118intergenic (+28/+82)ypfN/ypfHputative membrane protein, UPF0370 family/palmitoyl‑CoA esterase activity, uncertain physiological substrate
Reads supporting (aligned to +/- strand):  ref base A (55/55);  new base C (3/4);  total (59/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 119 total aligned reads displayed.

CAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTT  >  NC_000913/2592842‑2593139
                                                                                                                                                    |                                                                                                                                                     
cAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAg                                                                                                                                                      >  1:1261975/1‑150 (MQ=255)
     gcgTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGtt                                                                                                                                                 >  1:1560768/1‑150 (MQ=255)
      ggTCGACCATAAGAAATATCTCGCCAACAAGCCGGAGCTTCCACCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTa                                                                                                                                                <  1:763456/149‑1 (MQ=255)
         cGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcg                                                                                                                                             >  2:964428/1‑150 (MQ=255)
         cGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcg                                                                                                                                             <  1:172420/150‑1 (MQ=255)
          gACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGTTAAGGCGTTAAcgc                                                                                                                                            >  2:615237/1‑150 (MQ=255)
            ccATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAAcgccg                                                                                                                                          <  1:1459068/150‑1 (MQ=255)
                aaGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATc                                                                                                                                      >  1:739011/1‑150 (MQ=255)
                 aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                                                     <  2:1281165/150‑1 (MQ=255)
                 aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATcc                                                                                                                                     <  2:1281166/150‑1 (MQ=255)
                 aGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATcc                                                                                                                                     >  2:331062/1‑150 (MQ=255)
                    aaTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCATGATTTCAACGATAAGTGGGACGATGCCGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                                                                  <  1:174252/150‑1 (MQ=255)
                    aaTTTATCTCCTACAATCCTGATCTTCACCCGAATCGTGATTTCAAGGATAAGGGGGACGATGACGACGACTGCCCGAAAAAGGTTCAACCGAAGAAGTATGCTGAGTGCTCATCCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGc                                                                                                                                  <  1:45426/150‑1 (MQ=37)
                    aaTATCTCTCCAACAAGCCGGAGCTTCCCCCGCATCGTGAGTTCAACCTTCAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCTACCGAAGAAGTAAGCTGAGTGCTCAACCTTAGGCCGGATAAGGCGTTAACGCCGCTTCCGGc                                                                                                                                  <  1:41933/150‑1 (MQ=255)
                     aTTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATGCGGCGTTAACGCCTTATCCGGcc                                                                                                                                 <  1:523821/150‑2 (MQ=255)
                      tttCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGCGGACGGGCCGAAAGAGGCTCAACCGCTGAAGTGCGCGGCGTGGGGAACCGTAGGCCGGATTGGGCGTTGACGCCGCTGCCGGcga                                                                                                                                >  2:110604/1‑148 (MQ=255)
                        tctcGCCAACAAGCCGGAGATTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATg                                                                                                                              >  1:198457/1‑150 (MQ=255)
                         ctcGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATgg                                                                                                                             >  1:890324/1‑150 (MQ=255)
                         ctcGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATgg                                                                                                                             <  2:238377/150‑1 (MQ=255)
                           cGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGTCGATGACGACGACTGGCCGAAAAAGGATCAACCGCAGAAGGAAGCTGAGTGCTCAACCGTAGGCCGGATACGGCGTTAACGCCGCATCCGGCAATGGTg                                                                                                                           >  1:363826/1‑150 (MQ=255)
                           cGCCAACAAGCCGGAGCTTCCCCCGCATCGTGAGTTCAACGATAAGTGGGACGATGACGCCGACGGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGCGTGCTCAACCGTAGGCCGGATAAGGCGGTAACGCCGCATCCGGCAATGGTg                                                                                                                           >  1:357523/1‑150 (MQ=255)
                            gCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGa                                                                                                                          >  1:73779/1‑150 (MQ=255)
                                   aGCCGGAGCTTCCCCCTCATCTTGATTTCAACGATAAGTGGGAGGATGACGACGACTGGCCGTAATAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGc                                                                                                                   <  1:1008366/150‑1 (MQ=39)
                                   aGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGc                                                                                                                   >  1:1150747/1‑150 (MQ=255)
                                      cGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCATTAACGCCGCATCCGGCAATGGTGAACGATGCCTg                                                                                                                >  1:43853/1‑150 (MQ=255)
                                         aGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATg                                                                                                             <  2:52862/150‑1 (MQ=255)
                                           cTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCg                                                                                                           <  2:1560768/150‑1 (MQ=255)
                                              cccccGCATCGTGATTTCAACTATAAGTGGTACGATGACGACGACTGTCCGAAAACGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACg                                                                                                        <  2:317918/150‑1 (MQ=255)
                                               ccccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGc                                                                                                       >  2:936235/1‑150 (MQ=255)
                                               ccccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGAGCTCAACCGTAGGCCGTATAAGGCGTTAACGACGCATCCGGCAATGGAGAACGATGCCTGATGCGACGc                                                                                                       >  2:528390/1‑150 (MQ=255)
                                                cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCt                                                                                                      >  1:94701/1‑150 (MQ=255)
                                                cccGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCt                                                                                                      >  1:577732/1‑150 (MQ=255)
                                                    cATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcg                                                                                                  <  2:676883/150‑1 (MQ=255)
                                                      tCGTGATTTCAACGATAAGTGGGACTATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGCTGCCTGATTCGACGCTCAcgcg                                                                                                >  1:259613/1‑150 (MQ=255)
                                                      tCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCAcgcg                                                                                                <  2:407584/150‑1 (MQ=255)
                                                        gTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTc                                                                                              <  1:1018574/150‑1 (MQ=255)
                                                        gTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCAACGCTCCCGCGTc                                                                                              >  2:1519162/1‑150 (MQ=255)
                                                        gTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCAACGCTCACGCGTc                                                                                              >  2:1512660/1‑150 (MQ=255)
                                                         tGATTTCAACGATTAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAAACGAAGAAGTAAGCTGAGTGCTCAACCCTAGGCCGGATAAGGCGTTAACGCCGCATCCGGGAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCt                                                                                             >  2:331955/1‑150 (MQ=255)
                                                          gATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCtt                                                                                            >  2:944424/1‑150 (MQ=255)
                                                              tCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCa                                                                                        <  2:198457/150‑1 (MQ=255)
                                                               cAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAg                                                                                       >  2:82232/1‑150 (MQ=255)
                                                                 aCGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAAGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGc                                                                                     >  2:719308/1‑150 (MQ=255)
                                                                   gATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATAAGACGCGTGAGCGTCGCATCAGGcat                                                                                   <  1:1369527/150‑3 (MQ=37)
                                                                    aTAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAg                                                                                  <  2:739011/150‑1 (MQ=255)
                                                                    aTAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAg                                                                                  <  1:363397/150‑1 (MQ=255)
                                                                     aaaGTGTGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAgg                                                                                 <  1:1273120/149‑1 (MQ=255)
                                                                       aGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTa                                                                               >  2:1585194/1‑150 (MQ=255)
                                                                       aGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTa                                                                               <  1:331062/150‑1 (MQ=255)
                                                                          gggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATc                                                                            >  1:815144/1‑150 (MQ=255)
                                                                           ggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCt                                                                           >  2:1409844/1‑150 (MQ=255)
                                                                           ggACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCt                                                                           <  1:439297/150‑1 (MQ=255)
                                                                             ccGATTACGACGACAGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGt                                                                         <  1:311468/149‑1 (MQ=255)
                                                                               gATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCt                                                                       >  2:252348/1‑150 (MQ=255)
                                                                                aTGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTCGGCCGGATACGGCGTTAACCCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCtt                                                                      <  2:880218/150‑1 (MQ=255)
                                                                                  gacgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACACTCACGCGTCTTATCAGGCCAGGTAATCTGTCtttt                                                                    <  2:1162514/150‑1 (MQ=255)
                                                                                   acgacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCATTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTa                                                                   <  2:43853/150‑1 (MQ=255)
                                                                                     gacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAca                                                                 <  2:815005/150‑1 (MQ=255)
                                                                                     gacgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAca                                                                 <  2:1452797/150‑1 (MQ=255)
                                                                                      acgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcat                                                                >  1:504891/1‑150 (MQ=255)
                                                                                       cgacTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcatc                                                               >  1:325466/1‑150 (MQ=255)
                                                                                         acTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTAcatcat                                                             >  1:996706/1‑150 (MQ=255)
                                                                                          cTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCAtt                                                            <  1:82232/150‑1 (MQ=255)
                                                                                           tGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCAttt                                                           >  1:1002502/1‑150 (MQ=255)
                                                                                             gCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCa                                                         >  1:1549814/1‑150 (MQ=255)
                                                                                              ccGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCaa                                                        <  2:15325/150‑1 (MQ=255)
                                                                                              ccGAAAAAGGATCAACCGAAGAAGCCACCCGAGGCCTCAACCGTAGGCCGGATAAGGCGTTTACTCAGCATCAGGCAATTGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGCTAATCTGTCTTTTACATCATTTCaa                                                        <  1:331955/150‑1 (MQ=21)
                                                                                                gAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATc                                                      >  2:1153455/1‑150 (MQ=255)
                                                                                                  aaaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATcac                                                    <  1:964428/150‑1 (MQ=255)
                                                                                                   aaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATcaca                                                   >  1:955553/1‑150 (MQ=255)
                                                                                                   aaaGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATcaca                                                   <  2:1410429/150‑1 (MQ=255)
                                                                                                     aGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatc                                                 >  2:336606/1‑150 (MQ=255)
                                                                                                       gATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcat                                               >  2:1360809/1‑150 (MQ=255)
                                                                                                          cAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCAcatcatcat                                            >  2:1234666/1‑150 (MQ=255)
                                                                                                           aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACatcatcatc                                           >  1:1630512/1‑150 (MQ=255)
                                                                                                           aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACatcatcatc                                           >  1:1450617/1‑150 (MQ=255)
                                                                                                           aaCCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACatcatcatc                                           <  2:415742/150‑1 (MQ=255)
                                                                                                             ccGCAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGACGCATACTGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGc                                         <  1:120993/150‑1 (MQ=255)
                                                                                                             ccGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTATTCTGTCTTTTACATCATTTCAATCACATCATCATCGc                                         >  2:1578271/1‑150 (MQ=255)
                                                                                                               gaagaaGTAAGCTGAGTGCTCAACCGTAGGCCGTATAAGGCGTTAACGCAGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCa                                       <  2:1596989/150‑1 (MQ=255)
                                                                                                                      tAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTAc                                >  2:1632125/1‑150 (MQ=255)
                                                                                                                       aaGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTAcc                               >  1:1586322/1‑150 (MQ=255)
                                                                                                                       aaGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTGTTACATCATTTCAATCACATCATCATCGCCAGGCTTAcc                               >  2:213066/1‑150 (MQ=255)
                                                                                                                            gAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCg                              <  2:759629/146‑1 (MQ=255)
                                                                                                                             aGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACt                         >  2:797003/1‑150 (MQ=255)
                                                                                                                              gTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACtt                        <  2:1150747/150‑1 (MQ=255)
                                                                                                                                gCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAg                      >  2:1650450/1‑150 (MQ=255)
                                                                                                                                 cTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgc                     <  2:325466/150‑1 (MQ=255)
                                                                                                                                 cTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgc                     <  1:1409844/150‑1 (MQ=255)
                                                                                                                                 cTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgc                     >  2:1211633/1‑150 (MQ=255)
                                                                                                                                  tCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgcg                    <  2:1549814/150‑1 (MQ=255)
                                                                                                                                  tCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAgcg                    <  1:688312/150‑1 (MQ=255)
                                                                                                                                      accattgCCGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTGCAATCACAGCATCATCGCCAGGCTTACCGCCACTTAGCGCTtc                >  1:84537/7‑150 (MQ=38)
                                                                                                                                          aGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtcgtcg            <  2:275336/150‑1 (MQ=255)
                                                                                                                                          aGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtcgtcg            <  2:1040903/150‑1 (MQ=255)
                                                                                                                                          aGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTtcgtcg            <  2:1261975/150‑1 (MQ=255)
                                                                                                                                             ccGGATGCGGCGTTAACGCCTTATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGaaa         >  2:1567067/1‑150 (MQ=255)
                                                                                                                                              cGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAaat        <  1:936235/150‑1 (MQ=255)
                                                                                                                                                  tAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAaataatt    >  2:655763/1‑149 (MQ=255)
                                                                                                                                                    aGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCATACCGCCACTTAGCGCTTCGTCGAAATAATGtt  >  1:1057052/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAAGCGCGTCGACCATAAGAAATTTCTCGCCAACAAGCCGGAGCTTCCCCCGCATCGTGATTTCAACGATAAGTGGGACGATGACGACGACTGGCCGAAAAAGGATCAACCGAAGAAGTAAGCTGAGTGCTCAACCGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAATGGTGAACGATGCCTGATGCGACGCTCACGCGTCTTATCAGGCCAGGTAATCTGTCTTTTACATCATTTCAATCACATCATCATCGCCAGGCTTACCGCCACTTAGCGCTTCGTCGAAATAATGTT  >  NC_000913/2592842‑2593139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: