Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,599,239 T→A 8.2% K174N (AAA→AAT) ‡ dapA ← dihydrodipicolinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,599,2390TA 8.2% 252.3 / 10.0 97K174N (AAA→AAT) ‡dapAdihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base T (42/46);  new base A (6/2);  total (49/48)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

Only 100 of 103 total aligned reads displayed.

CCTTCTGCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAT  >  NC_000913/2599090‑2599387
                                                                                                                                                     |                                                                                                                                                    
ccTTCTGCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGctct                                                                                                                                                      >  1:907286/1‑150 (MQ=255)
 cTTCTGCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCtt                                                                                                                                                     <  1:816782/150‑1 (MQ=255)
    ctgctgCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGa                                                                                                                                                  <  2:352394/150‑1 (MQ=255)
     tgctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCGTTGAt                                                                                                                                                 >  2:584232/1‑150 (MQ=255)
      gctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATc                                                                                                                                                >  2:582508/1‑150 (MQ=255)
      gctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATc                                                                                                                                                >  2:610551/1‑150 (MQ=255)
       ctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCt                                                                                                                                               >  2:1023954/1‑150 (MQ=255)
           cAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGtt                                                                                                                                           <  1:1551508/150‑1 (MQ=255)
           cAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGtt                                                                                                                                           <  2:1624558/150‑1 (MQ=255)
            aGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGTAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCATAACAAAATCATCTGAAACCAGCTCTTTGATCTGGttt                                                                                                                                          >  2:852742/1‑150 (MQ=255)
            aGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGttt                                                                                                                                          <  1:610551/150‑1 (MQ=255)
                tGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTacac                                                                                                                                      >  1:607703/1‑150 (MQ=255)
                tGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTacac                                                                                                                                      <  1:642339/150‑1 (MQ=255)
                 gCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGGAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCAAAGAGCTGGtttcagat                                                                                                                                     <  1:702411/150‑6 (MQ=255)
                  cacaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgc                                                                                                                                    <  1:995153/150‑1 (MQ=255)
                  cacaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACAAGCTCTTTGATCTGGTTTacactc                                                                                                                                    >  2:1216209/1‑148 (MQ=255)
                   acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATCACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                   <  2:816647/150‑1 (MQ=255)
                   acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                   <  2:607703/150‑1 (MQ=255)
                   acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                   >  1:1180375/1‑150 (MQ=255)
                   acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                   <  2:371152/150‑1 (MQ=255)
                         gggCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCCGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCCGCTCTGTGATCTGGTTATCACGCGTTAAGt                                                                                                                             >  2:1558171/1‑150 (MQ=255)
                          ggCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGtt                                                                                                                            <  1:657854/150‑1 (MQ=255)
                           gCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGAATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGACCAAAATCATCTGAAACCAGCTCTTTGATCAGGTGTTCACGCGTTAAGTTc                                                                                                                           >  1:260830/1‑150 (MQ=255)
                            ccATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCCTGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGATcc                                                                                                                          <  2:1040254/150‑1 (MQ=255)
                               tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCACTg                                                                                                                       >  2:596676/1‑150 (MQ=255)
                                   aCGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGc                                                                                                                   >  2:634588/1‑150 (MQ=255)
                                    cgcgCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGcc                                                                                                                  >  1:464081/1‑150 (MQ=255)
                                      cgcTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCctc                                                                                                                <  1:642022/150‑1 (MQ=255)
                                        cTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCtt                                                                                                              <  1:511454/150‑1 (MQ=255)
                                         tGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCttt                                                                                                             <  2:470564/150‑1 (MQ=255)
                                           cGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGCACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGa                                                                                                           >  1:710846/1‑150 (MQ=255)
                                           cGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGa                                                                                                           <  1:504204/150‑1 (MQ=255)
                                             aCGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGAtt                                                                                                         <  2:1291987/150‑1 (MQ=255)
                                               gTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTcc                                                                                                       >  2:1346327/1‑150 (MQ=255)
                                               gTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTcc                                                                                                       <  2:2776/150‑1 (MQ=255)
                                               gTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGGTAAGTTCCCTGTTGCCTCTTTGATTcc                                                                                                       >  2:66461/1‑150 (MQ=255)
                                                 tAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGa                                                                                                     <  1:596676/150‑1 (MQ=255)
                                                  aGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCATCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGat                                                                                                    <  2:302335/150‑1 (MQ=255)
                                                      gTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGataata                                                                                                <  1:966254/150‑1 (MQ=255)
                                                          cGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttt                                                                                            >  1:1324318/1‑150 (MQ=255)
                                                           ggAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAttttt                                                                                           <  2:1296255/150‑1 (MQ=255)
                                                           ggAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAttttt                                                                                           <  2:464081/150‑1 (MQ=255)
                                                            taaaTAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCATCTCTTTGATCTGGTTTACACGCGTTTAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttttt                                                                                          <  1:695908/149‑1 (MQ=255)
                                                            gAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCTCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttttt                                                                                          <  1:94624/150‑1 (MQ=255)
                                                            gAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttttt                                                                                          <  2:150331/150‑1 (MQ=255)
                                                                tAACCCCATGACCGCCCAATTGCATGAAGTCAAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCACTGTTGCCTCTTTGATTCCGATAATATTTTTTTCtt                                                                                      >  1:134622/1‑150 (MQ=255)
                                                                   ccccATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCg                                                                                   <  1:192138/150‑1 (MQ=255)
                                                                   ccccATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCg                                                                                   <  1:625036/150‑1 (MQ=255)
                                                                    cccATGACCGCCCAATTGCATGAAGTGCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                                                  <  1:1097958/150‑1 (MQ=255)
                                                                       aTGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAg                                                                               <  1:1031215/150‑1 (MQ=255)
                                                                              cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                        >  2:659329/1‑150 (MQ=255)
                                                                              cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                        <  2:452225/150‑1 (MQ=255)
                                                                              cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                        >  2:426775/1‑150 (MQ=255)
                                                                                cAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCAc                                                                      <  1:1023954/150‑1 (MQ=255)
                                                                                     gCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGttt                                                                 >  1:1232802/1‑150 (MQ=255)
                                                                                     gCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAAACATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAACTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGttt                                                                 >  2:462226/1‑150 (MQ=255)
                                                                                       aTGAATTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAATATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTcc                                                               <  2:1505457/150‑1 (MQ=255)
                                                                                          aaGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                                            <  2:893006/150‑1 (MQ=255)
                                                                                           aGTCCAGCGCCCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTAGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTATCGCCAGACGGCCCACCGTTTCCGGGa                                                           >  2:891030/1‑150 (MQ=255)
                                                                                            gTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                                            <  2:1290517/148‑1 (MQ=255)
                                                                                            gTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                                            >  1:1290517/1‑148 (MQ=255)
                                                                                              ccAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCa                                                        >  1:1191380/1‑150 (MQ=255)
                                                                                                      cTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGAGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAg                                                >  2:733545/1‑150 (MQ=255)
                                                                                                        cGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCGTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCCCCGTTTCCGGGCGCAGATCGCAGcc                                              >  2:1254680/1‑150 (MQ=255)
                                                                                                          catcatCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAg                                            <  1:582508/150‑1 (MQ=255)
                                                                                                              atcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTAGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCAAGACGGCACACCGTTTCAGGGAGCAGATAGCAGCCAGTACg                                        >  1:1233386/1‑150 (MQ=255)
                                                                                                               tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACgg                                       <  1:66461/150‑1 (MQ=255)
                                                                                                                cgccgcTCAGCAGCACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACggg                                      >  1:1339815/1‑150 (MQ=255)
                                                                                                                    gcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGATAAGTTACCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCTCCAGACGGCCCACAGTTTCAGGGAGCAGCTCCAACCAAGTACGGGACgg                                  >  2:336819/1‑150 (MQ=255)
                                                                                                                    gcTCACCAGAACAAAATCATCTGAAACCAGCCCTTTGATCTGGTGTACACGCGTGAAGTACCCTGGTGCCTCTTTGATGCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTGCCGGGAGCAGATCGCAGCCAGTACGGGACgg                                  <  1:141110/150‑1 (MQ=255)
                                                                                                                       cagcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcac                               >  2:437773/1‑150 (MQ=255)
                                                                                                                       cagcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGAGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcac                               >  2:473182/1‑150 (MQ=255)
                                                                                                                         gcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACAt                             <  2:727335/150‑1 (MQ=255)
                                                                                                                         gcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACAt                             >  1:34709/1‑150 (MQ=255)
                                                                                                                            gAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCCGCCAGTACGGGACGGCACATtat                          >  1:1552642/1‑150 (MQ=255)
                                                                                                                                   atcatcTGAAACCAGCTCTTTCATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAAt                   <  1:1120623/150‑1 (MQ=255)
                                                                                                                                    tgatcTGAATCCAGTTCTTTGATGTGGTTTACTGGGGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCTGCCAGTACGGGACGGCACATTATACAGAttt                  <  1:1698807/148‑1 (MQ=255)
                                                                                                                                    tcatcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAAtt                  <  1:426775/150‑1 (MQ=255)
                                                                                                                                    cgcgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTTCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGCACGGCACATTATACAGAAtt                  >  2:50906/8‑150 (MQ=255)
                                                                                                                                     gcgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGCCGGCACATTATACAGAAttt                 >  1:1631496/7‑150 (MQ=255)
                                                                                                                                      cgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                >  2:1497525/6‑150 (MQ=255)
                                                                                                                                      cgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                >  2:457540/6‑150 (MQ=255)
                                                                                                                                      atcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                >  2:216246/1‑150 (MQ=255)
                                                                                                                                       tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGc               >  1:1415632/1‑150 (MQ=255)
                                                                                                                                       tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGAGAATATTTTTTACTTTCGCCCGACGGCCCCCCGTGTCCGGGAGCAGATCGCGGCCAGAACGGGACGGCACATTAGACAGACGTTGc               >  2:722679/1‑150 (MQ=255)
                                                                                                                                       gtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGc               >  2:238713/5‑150 (MQ=255)
                                                                                                                                        cTGAAACCCGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCGCACCGTTTCCGGGAGCAGATCGCCGCCAGTACGCGACGGCCCATTATAGAGAAGTTGCg              >  2:861933/1‑150 (MQ=255)
                                                                                                                                        cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCg              <  1:854588/150‑1 (MQ=255)
                                                                                                                                        cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCAAGTACGGGACGGCACATTATACAGAATTTGCg              >  1:1179605/1‑150 (MQ=255)
                                                                                                                                        cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGGTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAAGTTGCg              >  2:1021482/1‑150 (MQ=255)
                                                                                                                                         gtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGTCACATTATACAGAATTTGCgg             <  1:1497525/148‑1 (MQ=255)
                                                                                                                                           ataCCATCTCTTTGATCTGTTTTACACGCGTTAAGTTCACTGTTGCCTCTTTTCTTCAGAAAATATTTTTAACTTTAGCCAGACTTCCCACAGTTTCCGGTAGCAGATCGCAGACAGTACGGGACGGCACATTATACAGAATTTGCGGCa           <  2:1018318/148‑1 (MQ=255)
                                                                                                                                           aaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGAAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa           >  1:1700333/1‑150 (MQ=255)
                                                                                                                                            aaCCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAg          <  1:854338/150‑1 (MQ=255)
                                                                                                                                             accttctctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTTCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCTCCGTTTCCGCGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAgg         <  1:447152/145‑1 (MQ=255)
                                                                                                                                               cAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc       <  1:1062418/150‑1 (MQ=255)
                                                                                                                                                 gCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAg     <  2:624441/150‑1 (MQ=255)
                                                                                                                                                   tctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCTATAATATTTTTTACTTTCGCCAGACGGCCCACCTTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATAAAGAATTTGCGGCAGGTCAGTa   <  1:852742/150‑1 (MQ=255)
                                                                                                                                                   tctTTGATCTGGTTTACACGAGTTAATTTCCCTGTTTCATCTTTGTTTCCGATTTTATTTTTTACTTTCTCCAGACGGCCCACCGTTTCCGGGAGCAGATAGCAGCCAGTACGGGACGGCACATTATACTGAATTTGCGGCATGTCAGTa   <  2:1112393/150‑1 (MQ=255)
                                                                                                                                                    ctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAt  >  2:1168032/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCTTCTGCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAT  >  NC_000913/2599090‑2599387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: