Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,599,240 T→A 7.7% K174I (AAA→ATA) ‡ dapA ← dihydrodipicolinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,599,2400TA 7.7% 263.0 / 8.4 101K174I (AAA→ATA) ‡dapAdihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base T (43/49);  new base A (6/2);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 102 total aligned reads displayed.

CTTCTGCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAT  >  NC_000913/2599091‑2599387
                                                                                                                                                     |                                                                                                                                                   
cTTCTGCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCtt                                                                                                                                                     <  1:816782/150‑1 (MQ=255)
   ctgctgCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGa                                                                                                                                                  <  2:352394/150‑1 (MQ=255)
    tgctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCGTTGAt                                                                                                                                                 >  2:584232/1‑150 (MQ=255)
     gctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATc                                                                                                                                                >  2:582508/1‑150 (MQ=255)
     gctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATc                                                                                                                                                >  2:610551/1‑150 (MQ=255)
      ctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCt                                                                                                                                               >  2:1023954/1‑150 (MQ=255)
          cAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGtt                                                                                                                                           <  1:1551508/150‑1 (MQ=255)
          cAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGtt                                                                                                                                           <  2:1624558/150‑1 (MQ=255)
           aGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGTAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCATAACAAAATCATCTGAAACCAGCTCTTTGATCTGGttt                                                                                                                                          >  2:852742/1‑150 (MQ=255)
           aGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGttt                                                                                                                                          <  1:610551/150‑1 (MQ=255)
               tGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTacac                                                                                                                                      >  1:607703/1‑150 (MQ=255)
               tGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTacac                                                                                                                                      <  1:642339/150‑1 (MQ=255)
                gCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGGAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCAAAGAGCTGGtttcagat                                                                                                                                     <  1:702411/150‑6 (MQ=255)
                 cacaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgc                                                                                                                                    <  1:995153/150‑1 (MQ=255)
                 cacaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACAAGCTCTTTGATCTGGTTTacactc                                                                                                                                    >  2:1216209/1‑148 (MQ=255)
                  acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATCACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                   <  2:816647/150‑1 (MQ=255)
                  acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                   <  2:607703/150‑1 (MQ=255)
                  acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                   >  1:1180375/1‑150 (MQ=255)
                  acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                   <  2:371152/150‑1 (MQ=255)
                        gggCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCCGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCCGCTCTGTGATCTGGTTATCACGCGTTAAGt                                                                                                                             >  2:1558171/1‑150 (MQ=255)
                         ggCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGtt                                                                                                                            <  1:657854/150‑1 (MQ=255)
                          gCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGAATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGACCAAAATCATCTGAAACCAGCTCTTTGATCAGGTGTTCACGCGTTAAGTTc                                                                                                                           >  1:260830/1‑150 (MQ=255)
                           ccATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCCTGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGATcc                                                                                                                          <  2:1040254/150‑1 (MQ=255)
                              tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCACTg                                                                                                                       >  2:596676/1‑150 (MQ=255)
                                  aCGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGc                                                                                                                   >  2:634588/1‑150 (MQ=255)
                                   cgcgCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGcc                                                                                                                  >  1:464081/1‑150 (MQ=255)
                                     cgcTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCctc                                                                                                                <  1:642022/150‑1 (MQ=255)
                                       cTGCGACGTTAGCCGTAACGGAAATAACCCCATGCCCGCCCAAATGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCctcat                                                                                                              >  2:183289/1‑148 (MQ=255)
                                       cTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCtt                                                                                                              <  1:511454/150‑1 (MQ=255)
                                        tGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCttt                                                                                                             <  2:470564/150‑1 (MQ=255)
                                          cGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGCACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGa                                                                                                           >  1:710846/1‑150 (MQ=255)
                                          cGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGa                                                                                                           <  1:504204/150‑1 (MQ=255)
                                            aCGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGAtt                                                                                                         <  2:1291987/150‑1 (MQ=255)
                                              gTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTcc                                                                                                       <  2:2776/150‑1 (MQ=255)
                                              gTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTcc                                                                                                       >  2:1346327/1‑150 (MQ=255)
                                              gTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGGTAAGTTCCCTGTTGCCTCTTTGATTcc                                                                                                       >  2:66461/1‑150 (MQ=255)
                                                tAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGa                                                                                                     <  1:596676/150‑1 (MQ=255)
                                                 aGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCATCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGat                                                                                                    <  2:302335/150‑1 (MQ=255)
                                                     gTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGataata                                                                                                <  1:966254/150‑1 (MQ=255)
                                                         cGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttt                                                                                            >  1:1324318/1‑150 (MQ=255)
                                                          ggAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAttttt                                                                                           <  2:464081/150‑1 (MQ=255)
                                                          ggAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAttttt                                                                                           <  2:1296255/150‑1 (MQ=255)
                                                           taaaTAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCATCTCTTTGATCTGGTTTACACGCGTTTAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttttt                                                                                          <  1:695908/149‑1 (MQ=255)
                                                           gAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCTCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttttt                                                                                          <  1:94624/150‑1 (MQ=255)
                                                           gAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttttt                                                                                          <  2:150331/150‑1 (MQ=255)
                                                               tAACCCCATGACCGCCCAATTGCATGAAGTCAAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCACTGTTGCCTCTTTGATTCCGATAATATTTTTTTCtt                                                                                      >  1:134622/1‑150 (MQ=255)
                                                                  ccccATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCg                                                                                   <  1:192138/150‑1 (MQ=255)
                                                                  ccccATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCg                                                                                   <  1:625036/150‑1 (MQ=255)
                                                                   cccATGACCGCCCAATTGCATGAAGTGCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                                                  <  1:1097958/150‑1 (MQ=255)
                                                                      aTGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAg                                                                               <  1:1031215/150‑1 (MQ=255)
                                                                             cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                        >  2:659329/1‑150 (MQ=255)
                                                                             cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                        <  2:452225/150‑1 (MQ=255)
                                                                             cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                        >  2:426775/1‑150 (MQ=255)
                                                                               cAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCAc                                                                      <  1:1023954/150‑1 (MQ=255)
                                                                                    gCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGttt                                                                 >  1:1232802/1‑150 (MQ=255)
                                                                                    gCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAAACATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAACTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGttt                                                                 >  2:462226/1‑150 (MQ=255)
                                                                                      aTGAATTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAATATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTcc                                                               <  2:1505457/150‑1 (MQ=255)
                                                                                         aaGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                                            <  2:893006/150‑1 (MQ=255)
                                                                                          aGTCCAGCGCCCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTAGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTATCGCCAGACGGCCCACCGTTTCCGGGa                                                           >  2:891030/1‑150 (MQ=255)
                                                                                           gTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                                            <  2:1290517/148‑1 (MQ=255)
                                                                                           gTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                                            >  1:1290517/1‑148 (MQ=255)
                                                                                             ccAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCa                                                        >  1:1191380/1‑150 (MQ=255)
                                                                                                     cTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGAGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAg                                                >  2:733545/1‑150 (MQ=255)
                                                                                                       cGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCGTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCCCCGTTTCCGGGCGCAGATCGCAGcc                                              >  2:1254680/1‑150 (MQ=255)
                                                                                                         catcatCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAg                                            <  1:582508/150‑1 (MQ=255)
                                                                                                             atcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTAGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCAAGACGGCACACCGTTTCAGGGAGCAGATAGCAGCCAGTACg                                        >  1:1233386/1‑150 (MQ=255)
                                                                                                              tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACgg                                       <  1:66461/150‑1 (MQ=255)
                                                                                                               cgccgcTCAGCAGCACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACggg                                      >  1:1339815/1‑150 (MQ=255)
                                                                                                                   gcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGATAAGTTACCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCTCCAGACGGCCCACAGTTTCAGGGAGCAGCTCCAACCAAGTACGGGACgg                                  >  2:336819/1‑150 (MQ=255)
                                                                                                                   gcTCACCAGAACAAAATCATCTGAAACCAGCCCTTTGATCTGGTGTACACGCGTGAAGTACCCTGGTGCCTCTTTGATGCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTGCCGGGAGCAGATCGCAGCCAGTACGGGACgg                                  <  1:141110/150‑1 (MQ=255)
                                                                                                                      cagcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcac                               >  2:437773/1‑150 (MQ=255)
                                                                                                                      cagcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGAGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcac                               >  2:473182/1‑150 (MQ=255)
                                                                                                                        gcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACAt                             <  2:727335/150‑1 (MQ=255)
                                                                                                                        gcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACAt                             >  1:34709/1‑150 (MQ=255)
                                                                                                                           gAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCCGCCAGTACGGGACGGCACATtat                          >  1:1552642/1‑150 (MQ=255)
                                                                                                                                  atcatcTGAAACCAGCTCTTTCATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAAt                   <  1:1120623/150‑1 (MQ=255)
                                                                                                                                   tgatcTGAATCCAGTTCTTTGATGTGGTTTACTGGGGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCTGCCAGTACGGGACGGCACATTATACAGAttt                  <  1:1698807/148‑1 (MQ=255)
                                                                                                                                   tcatcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAAtt                  <  1:426775/150‑1 (MQ=255)
                                                                                                                                   cgcgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTTCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGCACGGCACATTATACAGAAtt                  >  2:50906/8‑150 (MQ=255)
                                                                                                                                    gcgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGCCGGCACATTATACAGAAttt                 >  1:1631496/7‑150 (MQ=255)
                                                                                                                                     cgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                >  2:1497525/6‑150 (MQ=255)
                                                                                                                                     cgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                >  2:457540/6‑150 (MQ=255)
                                                                                                                                     atcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                >  2:216246/1‑150 (MQ=255)
                                                                                                                                      tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGc               >  1:1415632/1‑150 (MQ=255)
                                                                                                                                      tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGAGAATATTTTTTACTTTCGCCCGACGGCCCCCCGTGTCCGGGAGCAGATCGCGGCCAGAACGGGACGGCACATTAGACAGACGTTGc               >  2:722679/1‑150 (MQ=255)
                                                                                                                                      gtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGc               >  2:238713/5‑150 (MQ=255)
                                                                                                                                       cTGAAACCCGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCGCACCGTTTCCGGGAGCAGATCGCCGCCAGTACGCGACGGCCCATTATAGAGAAGTTGCg              >  2:861933/1‑150 (MQ=255)
                                                                                                                                       cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCg              <  1:854588/150‑1 (MQ=255)
                                                                                                                                       cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCAAGTACGGGACGGCACATTATACAGAATTTGCg              >  1:1179605/1‑150 (MQ=255)
                                                                                                                                       cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGGTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAAGTTGCg              >  2:1021482/1‑150 (MQ=255)
                                                                                                                                        gtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGTCACATTATACAGAATTTGCgg             <  1:1497525/148‑1 (MQ=255)
                                                                                                                                         gAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGc            >  1:624441/1‑150 (MQ=255)
                                                                                                                                          aaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGAAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa           >  1:1700333/1‑150 (MQ=255)
                                                                                                                                           aaCCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAg          <  1:854338/150‑1 (MQ=255)
                                                                                                                                            accttctctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTTCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCTCCGTTTCCGCGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAgg         <  1:447152/145‑1 (MQ=255)
                                                                                                                                              cAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc       <  1:1062418/150‑1 (MQ=255)
                                                                                                                                                gCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAg     <  2:624441/150‑1 (MQ=255)
                                                                                                                                                  tctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCTATAATATTTTTTACTTTCGCCAGACGGCCCACCTTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATAAAGAATTTGCGGCAGGTCAGTa   <  1:852742/150‑1 (MQ=255)
                                                                                                                                                  tctTTGATCTGGTTTACACGAGTTAATTTCCCTGTTTCATCTTTGTTTCCGATTTTATTTTTTACTTTCTCCAGACGGCCCACCGTTTCCGGGAGCAGATAGCAGCCAGTACGGGACGGCACATTATACTGAATTTGCGGCATGTCAGTa   <  2:1112393/150‑1 (MQ=255)
                                                                                                                                                   ctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAt  >  2:1168032/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTTCTGCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAT  >  NC_000913/2599091‑2599387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: