Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,599,244 T→G 7.8% I173L (ATC→CTC)  dapA ← dihydrodipicolinate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,599,2440TG 7.8% 283.3 / 11.0 100I173L (ATC→CTC) dapAdihydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base T (43/49);  new base G (6/2);  total (49/51)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 101 total aligned reads displayed.

TGCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTC  >  NC_000913/2599095‑2599391
                                                                                                                                                     |                                                                                                                                                   
tgctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCGTTGAt                                                                                                                                                     >  2:584232/1‑150 (MQ=255)
 gctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATc                                                                                                                                                    >  2:610551/1‑150 (MQ=255)
 gctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATc                                                                                                                                                    >  2:582508/1‑150 (MQ=255)
  ctgcCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCt                                                                                                                                                   >  2:1023954/1‑150 (MQ=255)
      cAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGtt                                                                                                                                               <  1:1551508/150‑1 (MQ=255)
      cAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGtt                                                                                                                                               <  2:1624558/150‑1 (MQ=255)
       aGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGTAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCATAACAAAATCATCTGAAACCAGCTCTTTGATCTGGttt                                                                                                                                              >  2:852742/1‑150 (MQ=255)
       aGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGttt                                                                                                                                              <  1:610551/150‑1 (MQ=255)
           tGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTacac                                                                                                                                          >  1:607703/1‑150 (MQ=255)
           tGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTacac                                                                                                                                          <  1:642339/150‑1 (MQ=255)
            gCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGGAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGATCAAAGAGCTGGtttcagat                                                                                                                                         <  1:702411/150‑6 (MQ=255)
             cacaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgc                                                                                                                                        <  1:995153/150‑1 (MQ=255)
             cacaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACAAGCTCTTTGATCTGGTTTacactc                                                                                                                                        >  2:1216209/1‑148 (MQ=255)
              acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATCACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                       <  2:816647/150‑1 (MQ=255)
              acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                       >  1:1180375/1‑150 (MQ=255)
              acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                       <  2:607703/150‑1 (MQ=255)
              acaTCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACAcgcg                                                                                                                                       <  2:371152/150‑1 (MQ=255)
                    gggCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCCGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCCGCTCTGTGATCTGGTTATCACGCGTTAAGt                                                                                                                                 >  2:1558171/1‑150 (MQ=255)
                     ggCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGtt                                                                                                                                <  1:657854/150‑1 (MQ=255)
                      gCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGAATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGACCAAAATCATCTGAAACCAGCTCTTTGATCAGGTGTTCACGCGTTAAGTTc                                                                                                                               >  1:260830/1‑150 (MQ=255)
                       ccATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCCTGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGATcc                                                                                                                              <  2:1040254/150‑1 (MQ=255)
                          tatCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCACTg                                                                                                                           >  2:596676/1‑150 (MQ=255)
                              aCGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGc                                                                                                                       >  2:634588/1‑150 (MQ=255)
                               cgcgCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGcc                                                                                                                      >  1:464081/1‑150 (MQ=255)
                                 cgcTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCctc                                                                                                                    <  1:642022/150‑1 (MQ=255)
                                   cTGCGACGTTAGCCGTAACGGAAATAACCCCATGCCCGCCCAAATGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCctcat                                                                                                                  >  2:183289/1‑148 (MQ=255)
                                   cTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCtt                                                                                                                  <  1:511454/150‑1 (MQ=255)
                                    tGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCttt                                                                                                                 <  2:470564/150‑1 (MQ=255)
                                      cGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGCACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGa                                                                                                               >  1:710846/1‑150 (MQ=255)
                                      cGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGa                                                                                                               <  1:504204/150‑1 (MQ=255)
                                        aCGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGAtt                                                                                                             <  2:1291987/150‑1 (MQ=255)
                                          gTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTcc                                                                                                           <  2:2776/150‑1 (MQ=255)
                                          gTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTcc                                                                                                           >  2:1346327/1‑150 (MQ=255)
                                          gTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGGTAAGTTCCCTGTTGCCTCTTTGATTcc                                                                                                           >  2:66461/1‑150 (MQ=255)
                                            tAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGa                                                                                                         <  1:596676/150‑1 (MQ=255)
                                             aGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCATCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGat                                                                                                        <  2:302335/150‑1 (MQ=255)
                                                 gTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGataata                                                                                                    <  1:966254/150‑1 (MQ=255)
                                                     cGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttt                                                                                                >  1:1324318/1‑150 (MQ=255)
                                                      ggAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAttttt                                                                                               <  2:464081/150‑1 (MQ=255)
                                                      ggAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAttttt                                                                                               <  2:1296255/150‑1 (MQ=255)
                                                       taaaTAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCATCTCTTTGATCTGGTTTACACGCGTTTAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttttt                                                                                              <  1:695908/149‑1 (MQ=255)
                                                       gAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCTCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttttt                                                                                              <  1:94624/150‑1 (MQ=255)
                                                       gAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATAtttttt                                                                                              <  2:150331/150‑1 (MQ=255)
                                                           tAACCCCATGACCGCCCAATTGCATGAAGTCAAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCACTGTTGCCTCTTTGATTCCGATAATATTTTTTTCtt                                                                                          >  1:134622/1‑150 (MQ=255)
                                                              ccccATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCg                                                                                       <  1:625036/150‑1 (MQ=255)
                                                              ccccATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCg                                                                                       <  1:192138/150‑1 (MQ=255)
                                                               cccATGACCGCCCAATTGCATGAAGTGCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGc                                                                                      <  1:1097958/150‑1 (MQ=255)
                                                                  aTGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAg                                                                                   <  1:1031215/150‑1 (MQ=255)
                                                                         cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                            <  2:452225/150‑1 (MQ=255)
                                                                         cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                            >  2:659329/1‑150 (MQ=255)
                                                                         cccAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGccc                                                                            >  2:426775/1‑150 (MQ=255)
                                                                           cAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCAc                                                                          <  1:1023954/150‑1 (MQ=255)
                                                                                gCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGttt                                                                     >  1:1232802/1‑150 (MQ=255)
                                                                                gCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAAACATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAACTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGttt                                                                     >  2:462226/1‑150 (MQ=255)
                                                                                  aTGAATTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAATATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTcc                                                                   <  2:1505457/150‑1 (MQ=255)
                                                                                     aaGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                                                <  2:893006/150‑1 (MQ=255)
                                                                                      aGTCCAGCGCCCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTAGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTATCGCCAGACGGCCCACCGTTTCCGGGa                                                               >  2:891030/1‑150 (MQ=255)
                                                                                       gTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                                                >  1:1290517/1‑148 (MQ=255)
                                                                                       gTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCggg                                                                <  2:1290517/148‑1 (MQ=255)
                                                                                         ccAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCa                                                            >  1:1191380/1‑150 (MQ=255)
                                                                                                 cTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGAGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAg                                                    >  2:733545/1‑150 (MQ=255)
                                                                                                   cGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCGTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCCCCGTTTCCGGGCGCAGATCGCAGcc                                                  >  2:1254680/1‑150 (MQ=255)
                                                                                                   cGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGcc                                                  >  2:925682/1‑150 (MQ=255)
                                                                                                     catcatCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAg                                                <  1:582508/150‑1 (MQ=255)
                                                                                                         atcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTAGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCAAGACGGCACACCGTTTCAGGGAGCAGATAGCAGCCAGTACg                                            >  1:1233386/1‑150 (MQ=255)
                                                                                                          tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACgg                                           <  1:66461/150‑1 (MQ=255)
                                                                                                           cgccgcTCAGCAGCACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACggg                                          >  1:1339815/1‑150 (MQ=255)
                                                                                                               gcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGATAAGTTACCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCTCCAGACGGCCCACAGTTTCAGGGAGCAGCTCCAACCAAGTACGGGACgg                                      >  2:336819/1‑150 (MQ=255)
                                                                                                               gcTCACCAGAACAAAATCATCTGAAACCAGCCCTTTGATCTGGTGTACACGCGTGAAGTACCCTGGTGCCTCTTTGATGCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTGCCGGGAGCAGATCGCAGCCAGTACGGGACgg                                      <  1:141110/150‑1 (MQ=255)
                                                                                                                  cagcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcac                                   >  2:437773/1‑150 (MQ=255)
                                                                                                                  cagcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGAGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcac                                   >  2:473182/1‑150 (MQ=255)
                                                                                                                    gcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACAt                                 <  2:727335/150‑1 (MQ=255)
                                                                                                                    gcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACAt                                 >  1:34709/1‑150 (MQ=255)
                                                                                                                       gAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCCGCCAGTACGGGACGGCACATtat                              >  1:1552642/1‑150 (MQ=255)
                                                                                                                              atcatcTGAAACCAGCTCTTTCATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAAt                       <  1:1120623/150‑1 (MQ=255)
                                                                                                                               tgatcTGAATCCAGTTCTTTGATGTGGTTTACTGGGGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCTGCCAGTACGGGACGGCACATTATACAGAttt                      <  1:1698807/148‑1 (MQ=255)
                                                                                                                               tcatcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAAtt                      <  1:426775/150‑1 (MQ=255)
                                                                                                                               cgcgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTTCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGCACGGCACATTATACAGAAtt                      >  2:50906/8‑150 (MQ=255)
                                                                                                                                gcgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGCCGGCACATTATACAGAAttt                     >  1:1631496/7‑150 (MQ=255)
                                                                                                                                 cgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                    >  2:1497525/6‑150 (MQ=255)
                                                                                                                                 cgtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                    >  2:457540/6‑150 (MQ=255)
                                                                                                                                 atcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                    >  2:216246/1‑150 (MQ=255)
                                                                                                                                  tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGc                   >  1:1415632/1‑150 (MQ=255)
                                                                                                                                  tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGAGAATATTTTTTACTTTCGCCCGACGGCCCCCCGTGTCCGGGAGCAGATCGCGGCCAGAACGGGACGGCACATTAGACAGACGTTGc                   >  2:722679/1‑150 (MQ=255)
                                                                                                                                  gtgtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGc                   >  2:238713/5‑150 (MQ=255)
                                                                                                                                   cTGAAACCCGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCGCACCGTTTCCGGGAGCAGATCGCCGCCAGTACGCGACGGCCCATTATAGAGAAGTTGCg                  >  2:861933/1‑150 (MQ=255)
                                                                                                                                   cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCg                  <  1:854588/150‑1 (MQ=255)
                                                                                                                                   cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCAAGTACGGGACGGCACATTATACAGAATTTGCg                  >  1:1179605/1‑150 (MQ=255)
                                                                                                                                   cTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGGTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAAGTTGCg                  >  2:1021482/1‑150 (MQ=255)
                                                                                                                                    gtaaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGTCACATTATACAGAATTTGCgg                 <  1:1497525/148‑1 (MQ=255)
                                                                                                                                     gAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGc                >  1:624441/1‑150 (MQ=255)
                                                                                                                                      ataCCATCTCTTTGATCTGTTTTACACGCGTTAAGTTCACTGTTGCCTCTTTTCTTCAGAAAATATTTTTAACTTTAGCCAGACTTCCCACAGTTTCCGGTAGCAGATCGCAGACAGTACGGGACGGCACATTATACAGAATTTGCGGCa               <  2:1018318/148‑1 (MQ=255)
                                                                                                                                      aaaCCAGATCAAAGAGCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGAAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa               >  1:1700333/1‑150 (MQ=255)
                                                                                                                                       aaCCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAg              <  1:854338/150‑1 (MQ=255)
                                                                                                                                        accttctctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTTCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCTCCGTTTCCGCGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAgg             <  1:447152/145‑1 (MQ=255)
                                                                                                                                          cAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTc           <  1:1062418/150‑1 (MQ=255)
                                                                                                                                            gCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAg         <  2:624441/150‑1 (MQ=255)
                                                                                                                                              tctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCTATAATATTTTTTACTTTCGCCAGACGGCCCACCTTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATAAAGAATTTGCGGCAGGTCAGTa       <  1:852742/150‑1 (MQ=255)
                                                                                                                                              tctTTGATCTGGTTTACACGAGTTAATTTCCCTGTTTCATCTTTGTTTCCGATTTTATTTTTTACTTTCTCCAGACGGCCCACCGTTTCCGGGAGCAGATAGCAGCCAGTACGGGACGGCACATTATACTGAATTTGCGGCATGTCAGTa       <  2:1112393/150‑1 (MQ=255)
                                                                                                                                               ctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTAt      >  2:1168032/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGCTGCCAGTTTGCACATCTGGGCCATATCACGCGCTGCGACGTTAGCCGTAACGGAAATAACCCCATGACCGCCCAATTGCATGAAGTCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTC  >  NC_000913/2599095‑2599391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: