Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,612,832 T→C 9.7% V311V (GTT→GTC) ‡ hyfR → hydrogenase‑4 transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,612,8320TC 9.7% 300.9 / 20.5 113V311V (GTT→GTC) ‡hyfRhydrogenase‑4 transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (52/50);  new base C (4/7);  total (56/57)
Fisher's exact test for biased strand distribution p-value = 5.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.12e-01

Only 100 of 115 total aligned reads displayed.

AACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGG  >  NC_000913/2612683‑2612980
                                                                                                                                                     |                                                                                                                                                    
aaCGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATTCCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGtt                                                                                                                                                      <  2:931764/150‑1 (MQ=255)
   ggCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGc                                                                                                                                                   <  1:183425/150‑1 (MQ=255)
    gCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGCTCGCATCGCTATTGCCGTTGGCa                                                                                                                                                  >  1:1463756/1‑150 (MQ=255)
     cTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCaa                                                                                                                                                 <  1:437129/150‑1 (MQ=255)
     cTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCaa                                                                                                                                                 >  2:1238993/1‑150 (MQ=255)
     cTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACAGGGTGAATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCaa                                                                                                                                                 <  1:157972/150‑1 (MQ=255)
       gCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATg                                                                                                                                               >  2:1439849/1‑150 (MQ=255)
        cGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGc                                                                                                                                              >  2:1373742/1‑150 (MQ=255)
        cGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGc                                                                                                                                              <  1:757414/150‑1 (MQ=255)
        cGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGc                                                                                                                                              >  2:1692448/1‑150 (MQ=255)
         gAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGcc                                                                                                                                             <  2:1654456/150‑1 (MQ=255)
             cTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGGCATTGCCAACGGCAATAGCGATg                                                                                                                                         <  1:493236/150‑1 (MQ=255)
             cTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCAACGGCAATAGCGATg                                                                                                                                         <  1:205394/150‑1 (MQ=255)
             cTGCGCTCCTTATACAGCTTTCCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATg                                                                                                                                         <  2:419679/150‑1 (MQ=255)
              ttcgttCCTTATAGCTCTTAACTTTGTCAACCATACACCGGTTGTATTGTTGCTGGCGGAGACCTGTTCCACTCGCTTTAGTGAGGAAAACTGCCAGCTTCTACAACTCATAGCCGATCGCTTCGGCATTGCCAACGGCAATAGCGATGc                                                                                                                                        <  2:1049598/145‑1 (MQ=255)
              tGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGGCATTGCCAACGGCAATAGCGATGc                                                                                                                                        <  1:24476/150‑1 (MQ=255)
              tGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGGCATTGCCAACGGCAATAGCGATGc                                                                                                                                        <  2:555403/150‑1 (MQ=255)
              tGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCACATACCTCTTCCACGCTCTTTAGTGAGGATAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGc                                                                                                                                        >  1:492234/1‑150 (MQ=255)
                 gcTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTg                                                                                                                                     >  2:952581/1‑150 (MQ=255)
                   tCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGGCATTGCCAACGGCAATAGCGATGcgatcg                                                                                                                                   <  1:616489/150‑6 (MQ=38)
                   tCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGc                                                                                                                                   <  1:1121541/150‑1 (MQ=255)
                    ccTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCg                                                                                                                                  <  1:1317790/150‑1 (MQ=255)
                      ttATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTa                                                                                                                                <  1:575981/150‑1 (MQ=255)
                       tataCCGCTTACCTTTGGCAACCATACACCGGGTGCATTCTTGCTGGCGCATACCACTTCCACTCTATTTAGTGAGGAAAACTGCCAGCTACTACACCACATACCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAg                                                                                                                               >  2:89363/1‑150 (MQ=255)
                        ataCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGc                                                                                                                              <  2:58623/150‑1 (MQ=255)
                         taCCGCTTACCTTTGGCAGCCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCa                                                                                                                             >  1:1160720/1‑150 (MQ=255)
                         taCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCa                                                                                                                             <  2:790001/150‑1 (MQ=255)
                         taCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCa                                                                                                                             >  1:1632781/1‑150 (MQ=255)
                          aCCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCAt                                                                                                                            <  1:658154/150‑1 (MQ=255)
                             gCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGAc                                                                                                                         <  2:220986/150‑1 (MQ=255)
                             gCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGAc                                                                                                                         <  1:1373742/150‑1 (MQ=255)
                             gCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGAc                                                                                                                         >  2:1496260/1‑150 (MQ=255)
                             gCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGAc                                                                                                                         <  1:97730/150‑1 (MQ=255)
                              cTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGAcc                                                                                                                        <  1:1238993/150‑1 (MQ=255)
                                  ccTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAtt                                                                                                                    >  1:1022451/1‑150 (MQ=255)
                                   cTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGAttt                                                                                                                   >  2:399439/1‑150 (MQ=255)
                                    tttGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTg                                                                                                                  <  1:882166/150‑1 (MQ=255)
                                     ccagCAACAATGCACCCGGTGTATGGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATAGCTATTGCCTTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTgg                                                                                                                 >  2:138701/4‑149 (MQ=255)
                                        gCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAgg                                                                                                              <  2:1568019/150‑1 (MQ=255)
                                        gCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAgg                                                                                                              <  2:277312/150‑1 (MQ=255)
                                           aCCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGaaa                                                                                                           <  1:166966/150‑1 (MQ=255)
                                            ccATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAg                                                                                                          >  2:1396241/1‑150 (MQ=255)
                                            ccATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGACGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAg                                                                                                          <  1:1520524/150‑1 (MQ=255)
                                              aTACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGtt                                                                                                        >  1:679090/1‑150 (MQ=255)
                                                 cacCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTgc                                                                                                     <  2:1533986/150‑1 (MQ=255)
                                                 cacCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTgc                                                                                                     >  1:919934/1‑150 (MQ=255)
                                                  acCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTgca                                                                                                    <  2:1022451/150‑1 (MQ=255)
                                                   ccGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTgcag                                                                                                   <  1:1397753/150‑1 (MQ=255)
                                                    cGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTgcagc                                                                                                  >  1:725823/1‑150 (MQ=255)
                                                    cGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTgcagc                                                                                                  <  2:1655182/150‑1 (MQ=255)
                                                     gggTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTgcagca                                                                                                 <  1:1692448/150‑1 (MQ=255)
                                                      ggTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCaa                                                                                                >  2:571309/1‑150 (MQ=255)
                                                       gTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAg                                                                                               <  1:48570/150‑1 (MQ=255)
                                                       gTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAg                                                                                               <  1:1419096/150‑1 (MQ=255)
                                                          cATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGaaa                                                                                            >  1:708323/1‑150 (MQ=255)
                                                            ttgttgCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCg                                                                                                                                            >  2:789650/1‑100 (MQ=255)
                                                            ttgttgCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCg                                                                                                                                            <  1:789650/100‑1 (MQ=255)
                                                                    ggCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCt                                                                                  >  1:1697456/1‑150 (MQ=255)
                                                                     gcgcATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGAAGCAAGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCACCtt                                                                                 >  1:126962/1‑150 (MQ=255)
                                                                      cgcATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTa                                                                                >  2:204718/1‑150 (MQ=255)
                                                                       gcATACCTCTTCCACGCTATTTAGTGATGAAAACTGCCAGCTACTACAACACATAGCCTATCGCATAGCTATTGCCGTTGGCAATGCCGATGCCTTGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAg                                                                               <  1:1142978/150‑1 (MQ=255)
                                                                        cATAGCTCTTCCACGCACTATACTGAGGATAACTGCCTGCTACTACATCACATAGCCGATCGCATCGCTATTGACGGTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTCGc                                                                              <  2:264631/150‑1 (MQ=255)
                                                                        cATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGc                                                                              >  2:611498/1‑150 (MQ=255)
                                                                          tACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGa                                                                            >  2:1668294/1‑150 (MQ=255)
                                                                             ctctTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGagca                                                                         >  2:1370375/1‑150 (MQ=255)
                                                                                   cACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCt                                                                   <  2:1223953/150‑1 (MQ=255)
                                                                                       ctctTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCg                                                               >  2:620492/1‑150 (MQ=255)
                                                                                          tttAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAAt                                                            >  2:1183632/1‑150 (MQ=255)
                                                                                                gAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGc                                                      <  2:1335484/150‑1 (MQ=255)
                                                                                                 aGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCa                                                     >  1:760770/1‑150 (MQ=255)
                                                                                                  ggAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCAt                                                    <  1:1449935/150‑1 (MQ=255)
                                                                                                       aCTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGTTGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCATAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTg                                               <  2:469218/150‑1 (MQ=255)
                                                                                                       aCTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGTAGCTCCTTTCGAATCTGGGCATCGGTg                                               >  1:320105/1‑150 (MQ=255)
                                                                                                          gCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACa                                            >  1:1375036/1‑150 (MQ=255)
                                                                                                           ccAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACAt                                           >  1:1083488/1‑150 (MQ=255)
                                                                                                                    acaacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCa                                  >  1:443104/1‑150 (MQ=255)
                                                                                                                      aacaCATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCaaa                                >  1:101149/1‑150 (MQ=255)
                                                                                                                       acacATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAg                               <  2:679090/150‑1 (MQ=255)
                                                                                                                       acacATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAg                               <  1:1370375/150‑1 (MQ=255)
                                                                                                                       acacATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAg                               <  2:1425726/150‑1 (MQ=255)
                                                                                                                       acacATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGATTGCAGCAAGAAAACAACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAg                               >  2:1076982/1‑150 (MQ=255)
                                                                                                                       acacATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGAATGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCaaat                               >  1:1718960/1‑149 (MQ=255)
                                                                                                                          caTAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCa                            >  1:326943/1‑150 (MQ=255)
                                                                                                                              gCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCa                        >  1:522177/1‑150 (MQ=255)
                                                                                                                              gCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCa                        >  2:1294500/1‑150 (MQ=255)
                                                                                                                                gccaggcatcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAAt                      >  2:123358/6‑150 (MQ=38)
                                                                                                                                   aggcatcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGa                   >  1:880585/3‑150 (MQ=255)
                                                                                                                                    tgcatcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATATGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGaa                  >  1:1011008/2‑150 (MQ=255)
                                                                                                                                    cgcatcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGaa                  <  1:611498/150‑1 (MQ=255)
                                                                                                                                    cgcatcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGaa                  >  1:266384/1‑150 (MQ=255)
                                                                                                                                     gcatcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAg                 >  2:649927/1‑150 (MQ=255)
                                                                                                                                      catcggCATTGCCAACGGCAATAGCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGa                >  1:1662929/1‑150 (MQ=255)
                                                                                                                                       atcgcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGAc               <  1:1496260/150‑1 (MQ=255)
                                                                                                                                          gcTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTg            >  2:1336127/1‑150 (MQ=255)
                                                                                                                                              ttGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTcc        <  1:1294500/150‑1 (MQ=255)
                                                                                                                                               tGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCCCCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCAAGGCAATGGAAGACCTGCTccc       >  2:1296647/1‑149 (MQ=255)
                                                                                                                                                  cGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagca    <  2:725823/150‑1 (MQ=255)
                                                                                                                                                  cGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagca    <  1:1439849/150‑1 (MQ=255)
                                                                                                                                                   gTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCcagcag   <  1:571309/150‑1 (MQ=255)
                                                                                                                                                    ttGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTGGAGCAgg  <  2:1013721/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AACGGCTGCGAATCTGCGCTCCTTATACCGCTTACCTTTGGCAACCATACACCGGGTGCATTGTTGCTGGCGCATACCTCTTCCACTCTCTTTAGTGAGGAAAACTGCCAGCTACTACAACACATAGCCGATCGCATCGCTATTGCCGTTGGCAATGCCGATGCCTGGCGTAGCATGACCGATTTGCAGGAAAGTTTGCAGCAAGAAAACCACCAGCTTAGCGAGCAGCTCCTTTCGAATCTGGGCATCGGTGACATTATCTATCAAAGCCAGGCAATGGAAGACCTGCTCCAGCAGG  >  NC_000913/2612683‑2612980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: