Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,630,921 A→T 18.5% intergenic (+56/+37) yfgI → / ← guaA nalidixic acid resistance protein, putative periplasmic protein/GMP synthetase (glutamine aminotransferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,630,9210AT18.5% 220.7 / 38.7 104intergenic (+56/+37)yfgI/guaAnalidixic acid resistance protein, putative periplasmic protein/GMP synthetase (glutamine aminotransferase)
Reads supporting (aligned to +/- strand):  ref base A (49/34);  new base T (8/11);  total (57/47)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTAC  >  NC_000913/2630773‑2631069
                                                                                                                                                     |                                                                                                                                                    
gACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTaa                                                                                                                                                     >  2:1149281/1‑150 (MQ=255)
  cGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACa                                                                                                                                                   <  2:1513811/150‑1 (MQ=255)
  cGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGAAGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACa                                                                                                                                                   <  2:907431/150‑1 (MQ=255)
    ccAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCATATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACaga                                                                                                                                                 >  1:1142464/1‑150 (MQ=255)
        cAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAATCAGAAGAGAACAAATCGGACAGCTTGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGt                                                                                                                                             <  1:369892/150‑1 (MQ=255)
        cAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGt                                                                                                                                             <  2:65775/150‑1 (MQ=255)
        cAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGt                                                                                                                                             <  1:222482/150‑1 (MQ=255)
        cAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGt                                                                                                                                             <  2:588583/150‑1 (MQ=255)
        cAGAAATATGACTGAAGGCGGGCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGt                                                                                                                                             <  1:203040/150‑1 (MQ=255)
            aaTATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGttttt                                                                                                                                         <  1:515226/150‑2 (MQ=255)
             atatGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGtttttt                                                                                                                                        <  2:693626/150‑3 (MQ=255)
             atatGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGt                                                                                                                                        <  2:948060/150‑1 (MQ=255)
             atatGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGt                                                                                                                                        <  2:941929/150‑1 (MQ=255)
              tatGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCAATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGtt                                                                                                                                       <  2:91066/150‑1 (MQ=255)
              tatGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAACAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGtttttta                                                                                                                                       <  1:197818/150‑4 (MQ=255)
               atGTCTGAATGCGTTCTCTATCTGATGAAAAGAGTGGGCAAATCAGAAGAGATCAAATGGGAGAGGTGTTTTAATTAATCGATGTTAGTAACTTGAATCCTTTAATTCTTTAAGATAAAATACCCTCTGTTAGTACAGAGGGttttttat                                                                                                                                      <  2:1551642/150‑5 (MQ=255)
                tGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttca                                                                                                                                     >  2:678090/1‑150 (MQ=255)
                 gACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcat                                                                                                                                    <  2:602840/150‑1 (MQ=255)
                    tGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcattca                                                                                                                                 >  1:839316/1‑150 (MQ=255)
                     gAAGGCGGTCGCTATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTtcattcat                                                                                                                                <  1:168668/150‑1 (MQ=255)
                     gAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTtcattcat                                                                                                                                >  2:1270497/1‑150 (MQ=255)
                     gAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTtcattcat                                                                                                                                >  1:13579/1‑150 (MQ=255)
                           ggTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCa                                                                                                                          >  2:115367/1‑150 (MQ=255)
                            gTCGCGATCTGATGAAAAGCGTGGGCAAAGTAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAg                                                                                                                         >  2:1692660/1‑150 (MQ=255)
                            gTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAg                                                                                                                         <  1:854173/150‑1 (MQ=255)
                              cgcgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAggg                                                                                                                       <  2:458825/150‑1 (MQ=255)
                                    cTGATGTAAAGCGTGGGCAAAGCAGAAGAGACCAAATCGTACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAAt                                                                                                                 <  2:1142464/150‑1 (MQ=255)
                                    cTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAAt                                                                                                                 >  1:1525423/1‑150 (MQ=255)
                                     tgatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCGATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATc                                                                                                                <  1:8590/150‑1 (MQ=255)
                                     tgatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATc                                                                                                                <  1:1270497/150‑1 (MQ=255)
                                     tgatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATc                                                                                                                >  2:190886/1‑150 (MQ=255)
                                     tgatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACACTCTGTTGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATc                                                                                                                >  1:846972/1‑150 (MQ=255)
                                      gatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCa                                                                                                               <  1:450416/150‑1 (MQ=255)
                                          aaaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTcc                                                                                                           >  2:717611/1‑150 (MQ=255)
                                           aaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAATATAAAAAACCCTCTGTAGTAAAAGAGGGTTTTGTTCATTCATAGTTCAGGGTCAAATCATTccc                                                                                                          <  1:738238/150‑1 (MQ=255)
                                             aGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCAc                                                                                                        <  1:1180854/150‑1 (MQ=255)
                                                     cAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGt                                                                                                >  2:113301/1‑150 (MQ=255)
                                                      aaaGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTa                                                                                               <  1:471005/150‑1 (MQ=255)
                                                        aGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAg                                                                                              <  1:573670/149‑1 (MQ=255)
                                                        aGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAg                                                                                              >  2:573670/1‑149 (MQ=255)
                                                          cAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACACTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTg                                                                                           >  1:1214951/1‑150 (MQ=255)
                                                           agaagaGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTgg                                                                                          >  2:187708/1‑150 (MQ=255)
                                                              agagAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAAAAGAGGGTTTTTTTCATTCATATTGAAGGGAAAAATAATTCACAATAAATGGTAGATtgcgg                                                                                       >  1:18529/1‑150 (MQ=255)
                                                                agaACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCt                                                                                     >  2:43229/1‑150 (MQ=255)
                                                                     acaTCGGACAGCTGGTTTACTTACTCGATGGTAGTCACTTCACTCCTCGACTGCTTGCAGATACACAACCCTCTGTCGTCCCAGAGGGTTTTGTGCATTCCTAGTGCCGGGGCACATCATTCCCACTCATTGGTAGCTGGCGGCTTGCCg                                                                                <  1:215481/148‑1 (MQ=255)
                                                                       aTCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAATTCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCt                                                                              <  1:115367/150‑1 (MQ=255)
                                                                       aTCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCt                                                                              <  2:72567/150‑1 (MQ=255)
                                                                          ggACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGAt                                                                           >  1:838221/1‑150 (MQ=255)
                                                                               gCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCAt                                                                      <  1:164486/150‑1 (MQ=255)
                                                                               gCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCAt                                                                      >  2:684093/1‑150 (MQ=255)
                                                                                cTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATa                                                                     <  2:65164/150‑1 (MQ=255)
                                                                                cTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGATGGCGGCTTGCCGCTGATGTCATa                                                                     >  2:38397/1‑150 (MQ=255)
                                                                                       aattaatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCAcg                                                              >  1:1273478/1‑150 (MQ=255)
                                                                                           aatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCggg                                                          <  2:839316/150‑1 (MQ=255)
                                                                                           aatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGATGATGTCATACAACACGCggg                                                          >  1:881399/1‑150 (MQ=255)
                                                                                              cGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGaaa                                                       >  2:1022731/1‑150 (MQ=255)
                                                                                                aTGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATa                                                     >  1:1345381/1‑150 (MQ=255)
                                                                                                   ttAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCg                                                  >  1:1248083/1‑150 (MQ=255)
                                                                                                   ttAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCg                                                  >  1:1260056/1‑150 (MQ=255)
                                                                                                         aCTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACt                                            >  1:811138/1‑150 (MQ=255)
                                                                                                         aCTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCATGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACt                                            >  1:640988/1‑150 (MQ=255)
                                                                                                          cTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACAACGCGGGAAATACAGTTCACtt                                           >  1:1032572/1‑150 (MQ=255)
                                                                                                            tCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGGCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATTCACCACGCGGGAAATACCGTTCACTTCa                                         <  1:49736/150‑1 (MQ=255)
                                                                                                              aaTCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAtt                                       <  1:981950/150‑1 (MQ=255)
                                                                                                              aaTCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAtt                                       <  2:1463492/150‑1 (MQ=255)
                                                                                                               aTCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTg                                      <  1:574542/150‑1 (MQ=255)
                                                                                                                tCCTATAATTCTTTAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCAACTCAATGGTAGCTGGAGGATTGCAACTTATGTCATACAACACGCGGGAAATACCGTTCACTTCATTGa                                     >  1:1694518/1‑150 (MQ=255)
                                                                                                                tCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGa                                     <  1:1174041/150‑1 (MQ=255)
                                                                                                                 ccTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGAt                                    <  1:389502/150‑1 (MQ=255)
                                                                                                                 ccTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGAt                                    >  1:1613206/1‑150 (MQ=255)
                                                                                                                     taATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGTAAATACCGTTCACTTCATTGATAATg                                <  2:1273478/150‑1 (MQ=255)
                                                                                                                       aTTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCg                              >  1:1294205/1‑150 (MQ=255)
                                                                                                                       aTTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCg                              >  1:979477/1‑150 (MQ=255)
                                                                                                                        ttCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCgg                             <  2:1525423/150‑1 (MQ=255)
                                                                                                                          cTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGtt                           >  1:617449/1‑150 (MQ=255)
                                                                                                                          cTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGtt                           >  2:180867/1‑150 (MQ=255)
                                                                                                                               atgaacaaaaCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATTCCGTTCACTTCATTGATAATGCAGTTGGaaa                      >  2:850545/7‑150 (MQ=255)
                                                                                                                               aGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGaaa                      >  1:1163103/1‑150 (MQ=255)
                                                                                                                               aGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGaaa                      <  1:602226/150‑1 (MQ=255)
                                                                                                                                gATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAc                     >  2:204333/1‑150 (MQ=255)
                                                                                                                                  tAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAcgc                   >  1:1335942/1‑150 (MQ=255)
                                                                                                                                   aaaaaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAcgcg                  >  2:1316130/1‑150 (MQ=255)
                                                                                                                                   aaaaaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTAATAGTGCAGGGTCAAAACATTCACACTCAATCGTAGCTGGAGGATTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAcgcg                  >  1:64529/1‑150 (MQ=255)
                                                                                                                                    caaaaCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGa                 >  1:22684/2‑150 (MQ=255)
                                                                                                                                    aaaaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTCGGAAACGCGa                 >  1:1001790/1‑150 (MQ=255)
                                                                                                                                       aaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCg              >  1:1479898/1‑150 (MQ=255)
                                                                                                                                        aCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTg                          >  1:691457/1‑137 (MQ=255)
                                                                                                                                        aCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGa             >  1:957798/1‑150 (MQ=255)
                                                                                                                                        aCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGa             >  1:411973/1‑150 (MQ=255)
                                                                                                                                        aCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGa             >  1:515587/1‑150 (MQ=255)
                                                                                                                                        aCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGa             >  2:331268/1‑150 (MQ=255)
                                                                                                                                        aCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACACAATGCTAGCTGGCTTCTTGCCGAATATGTCATACACAACGCGGGAAATAACTTTCACATCATTGATAATGCGGTTGGAAACGCGACCGa             >  1:283760/1‑150 (MQ=255)
                                                                                                                                          ccTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAgg           <  1:987446/150‑1 (MQ=255)
                                                                                                                                           ctctGTAGTAACAGAGGGTTTTGTTGATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGAAGCTGATGTCATACACCACCCGGGAAATTCCGTTCACTTCATTGATAATGCGGTTGGAACCGCGACCGAGGa          >  2:300335/1‑150 (MQ=255)
                                                                                                                                               gtagtaACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATc      >  1:1162528/1‑150 (MQ=255)
                                                                                                                                                 tagtaACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCg     <  1:1149281/150‑1 (MQ=255)
                                                                                                                                                   gtaACAGAGGGTTTTGTTCATTCATAGTGAAGGCTCAAATCATTCCCACTCAATGGTAGCTGGCGGCAAGCCGGTTATGTCATACACCACGCGGGAAATATCGTTCACTTCATTGATAATGCGCTAGGAAACGCGACCGAGGAAATCGTa   >  2:536106/1‑150 (MQ=255)
                                                                                                                                                    taACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAc                     >  2:1390196/1‑131 (MQ=255)
                                                                                                                                                    taACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAAc                     <  1:1390196/131‑1 (MQ=255)
                                                                                                                                                    taACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTAc  <  2:13579/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATCGTAC  >  NC_000913/2630773‑2631069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: