Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,658,034 T→A 5.3% N301I (AAT→ATT)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,658,0340TA 5.3% 327.9 / 3.2 110N301I (AAT→ATT) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base T (55/47);  new base A (2/4);  total (59/51)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

ATTCGCCTACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTG  >  NC_000913/2657885‑2658182
                                                                                                                                                     |                                                                                                                                                    
aTTCGCCTACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCAt                                                                                                                                                      <  2:661126/150‑1 (MQ=255)
 ttCGCCTACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCAtt                                                                                                                                                     <  1:1296778/150‑1 (MQ=255)
 ttCGCCTACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCAtt                                                                                                                                                     <  1:1358296/150‑1 (MQ=255)
  tCGCCTAGACGTTCACGCACCAGCTGCACGCGAGTAGAACCGCCCACCATCACCAGTTCCCGCACTTCTTCAGCATCTACACGCACGTCTTGCTGCGCGCGAGGACATGCCTGTATGGTTCGTTTAACCAGTGTCGCGATCAGTTCATTg                                                                                                                                                    <  1:83356/150‑1 (MQ=255)
  tCGCCTACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCTCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTg                                                                                                                                                    <  2:614091/150‑1 (MQ=255)
    gCCTACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGaa                                                                                                                                                  >  2:1363864/1‑150 (MQ=255)
      cTACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAAtt                                                                                                                                                <  1:553834/150‑1 (MQ=255)
      cTACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAAtt                                                                                                                                                >  2:1318254/1‑150 (MQ=255)
      cTACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTGTACCAGTGGCGCGATCAGTTCATTGAAtt                                                                                                                                                >  1:1453721/1‑150 (MQ=255)
       tACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGGTCGTTTTACCAGTGGCGCGATCAGTTCAGTGAAtgg                                                                                                                                               >  2:112722/1‑148 (MQ=255)
       aaCCCTTTCACGCACCACCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACTACAAGCCAGTAATGTTCGTTTTACCAGTGGCGCGATCAGTTCATTTAATTg                                                                                                                                               <  1:1038582/149‑1 (MQ=255)
        aCCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGt                                                                                                                                              >  1:1456118/1‑150 (MQ=255)
         cccGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGtt                                                                                                                                             <  1:1363864/150‑1 (MQ=255)
          ccGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTc                                                                                                                                            <  2:212602/150‑1 (MQ=255)
          ccGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTc                                                                                                                                            <  2:21628/150‑1 (MQ=255)
           cGTTCACGCACCAGCGGCACGCGAGTAGACCCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTGTTTCAGCGCGCGACGACAAGCCAGGAAGGTACGTATTACCAGTGGCGCGATCAGATCATTGAAATGTTCa                                                                                                                                           >  2:838150/1‑150 (MQ=255)
                aCGCACCAGCGGCGCGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTGAGAGGGCGACGACAAGCCAGTAAGGTTCGTTTTAGCAGTGGCGCGATCAGTTCAGTGAATTGTTCACGGCt                                                                                                                                      >  2:618821/1‑150 (MQ=255)
                 cacACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTg                                                                                                                                     >  1:672819/3‑150 (MQ=255)
                 cGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTg                                                                                                                                     >  2:1384303/1‑150 (MQ=255)
                 cGCACCAGCGGCACGAGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTACGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTg                                                                                                                                     >  1:1132994/1‑150 (MQ=255)
                   cACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGAt                                                                                                                                   >  1:988590/1‑150 (MQ=255)
                     ccAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGAttt                                                                                                                                 <  1:112722/150‑1 (MQ=255)
                        gCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGGGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGc                                                                                                                              <  1:842131/150‑1 (MQ=255)
                         cGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGcc                                                                                                                             <  2:570928/150‑1 (MQ=255)
                            cACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGCCAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTg                                                                                                                          >  1:1454299/1‑150 (MQ=255)
                            cACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTg                                                                                                                          <  2:1588731/150‑1 (MQ=255)
                            cACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTGCACGGCTGATTTCGCCCTg                                                                                                                          <  1:1260712/150‑1 (MQ=255)
                            cACGCGAGTAGAACCGCCCACAATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTg                                                                                                                          >  1:599195/1‑150 (MQ=255)
                                cgAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGTGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCac                                                                                                                      >  2:744699/1‑149 (MQ=255)
                                     aGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGc                                                                                                                 >  1:168369/1‑150 (MQ=255)
                                        aCCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAAc                                                                                                              <  1:1384303/150‑1 (MQ=255)
                                           gCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCAATGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGtt                                                                                                           >  1:475787/1‑150 (MQ=255)
                                           gCCCACCATCACCACTTACAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGAAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCAATGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGtt                                                                                                           >  1:482234/1‑150 (MQ=255)
                                              caccaTCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAAc                                                                                                        <  1:524057/150‑1 (MQ=255)
                                               accaTCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACg                                                                                                       >  2:434319/1‑150 (MQ=255)
                                                  aTCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTc                                                                                                    <  2:785755/150‑1 (MQ=255)
                                                        acTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAg                                                                                              >  1:1474502/1‑150 (MQ=255)
                                                         cTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGt                                                                                             >  1:778152/1‑150 (MQ=255)
                                                         cTTCCAGCACTTCATCAGCGTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGCTCAGTTCATTGCATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGt                                                                                             >  1:1540864/1‑150 (MQ=255)
                                                          ttCCAGCACTTCATCAGCTTCTACACCCGCGGCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCGTTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTc                                                                                            <  1:676259/150‑1 (MQ=255)
                                                               ccACTTCAGCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACTACAAGCCAGTTATGTTCGTATTACCAGTGGCGCGATCATTTCAATGAATAGTCCACGGCTGCTTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCAt                                                                                       <  2:279676/149‑1 (MQ=255)
                                                                 aCTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCg                                                                                     <  1:95654/150‑1 (MQ=255)
                                                                 aCTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCg                                                                                     <  1:994597/150‑1 (MQ=255)
                                                                    tcatcaGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTc                                                                                  >  2:684600/1‑150 (MQ=255)
                                                                       tcaGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAgc                                                                               >  1:228919/1‑150 (MQ=255)
                                                                        caGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAgcg                                                                              <  2:672819/150‑1 (MQ=255)
                                                                         aGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAgcgc                                                                             >  2:972295/1‑150 (MQ=255)
                                                                          gCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGcgcg                                                                            >  1:921194/1‑150 (MQ=255)
                                                                                acacCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTg                                                                      >  2:590513/1‑150 (MQ=255)
                                                                                   cccGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCt                                                                   >  2:1511532/1‑150 (MQ=255)
                                                                                   cccGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCt                                                                   >  2:1403524/1‑150 (MQ=255)
                                                                                   cccGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCt                                                                   >  1:871194/1‑150 (MQ=255)
                                                                                    ccGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTg                                                                  >  2:1468999/1‑150 (MQ=255)
                                                                                    ccGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTg                                                                  <  1:1425395/150‑1 (MQ=255)
                                                                                    ccGCGTCTTTCAGCGAGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGTGTCCGCATCGCTCAGCGCGTTTTTGGCTg                                                                  <  2:1132994/150‑1 (MQ=255)
                                                                                      gcgTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCa                                                                >  2:1118494/1‑150 (MQ=255)
                                                                                          cTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGATTTGTTCACGGCTGATTTCGCACTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATgg                                                            >  2:866866/1‑150 (MQ=255)
                                                                                            ttCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCTCATCGCTCAGCGCGATTTTGGCTGCAATggcg                                                          <  2:871194/150‑1 (MQ=255)
                                                                                            ttCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATggcg                                                          >  2:173483/1‑150 (MQ=255)
                                                                                             tCAGCGCGCGACGACAAGCCATTAAGGTGCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATggcgg                                                         <  2:1502105/150‑1 (MQ=255)
                                                                                             tCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATggcgg                                                         >  2:1393707/1‑150 (MQ=255)
                                                                                             tCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATggcgg                                                         <  2:1456118/150‑1 (MQ=255)
                                                                                             tCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATggcgg                                                         <  2:778152/150‑1 (MQ=255)
                                                                                               aGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCa                                                       >  1:646545/1‑150 (MQ=255)
                                                                                                  gcgcgACGCCAAGCCAGAAAGGTTCGTTATACCAGTGGAGCGATCAGATCATTGAATAGTTCACGGCTGATGTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCTGCATcc                                                    <  1:838150/150‑1 (MQ=255)
                                                                                                  gcgcgACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTAAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATcc                                                    <  2:1553914/150‑1 (MQ=255)
                                                                                                       acgacAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGATCATTGAATAGTACACGGCTGATTTCGCCCTGCCAGCCCGCAACCTTAACGCTCACGGAGTCAGCATCGCTCAGCGCGATTTTTGCTGCAATTGCGGCATCcagcag                                               >  1:136632/1‑150 (MQ=255)
                                                                                                           cAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCa                                           >  2:928262/1‑150 (MQ=255)
                                                                                                               ccAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGtttaagcc                                       <  2:1574021/150‑7 (MQ=255)
                                                                                                               ccAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGtttaagcc                                       <  2:826989/150‑7 (MQ=255)
                                                                                                                     aGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAAcgc                                 >  2:110022/1‑150 (MQ=255)
                                                                                                                      ggTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAAcgcg                                <  2:27283/150‑1 (MQ=255)
                                                                                                                       gTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCgg                               >  2:1665260/1‑150 (MQ=255)
                                                                                                                       gTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCgg                               >  2:1671769/1‑150 (MQ=255)
                                                                                                                       gTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCgg                               >  1:477318/1‑150 (MQ=255)
                                                                                                                          cGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTa                            <  2:228919/150‑1 (MQ=255)
                                                                                                                           gTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTAt                           >  2:357954/1‑150 (MQ=255)
                                                                                                                            ttttACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATc                          >  1:1488192/1‑150 (MQ=255)
                                                                                                                            ttttACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTATGGCTGCTATGGCGCCAGCCAGCTCTTCACGCTGCCCGCGGTTATc                          >  1:1092916/1‑150 (MQ=255)
                                                                                                                            ttttACCAGTGGCGCGATCAGTTCAATGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATc                          <  2:475787/150‑1 (MQ=255)
                                                                                                                            ttttACCAGTGGCGCGATCAGTTCAATGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATc                          <  2:482234/150‑1 (MQ=255)
                                                                                                                            ttttACCAGTGGCGCGATCAGATCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATc                          <  1:231387/150‑1 (MQ=255)
                                                                                                                               tACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTTGCTGCAAAGGCGGCATCCAGCAGTAAACGCTCAACGCGGTTATCGCt                       >  1:115771/1‑150 (MQ=255)
                                                                                                                               tACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCt                       <  2:1535429/150‑1 (MQ=255)
                                                                                                                               tACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCt                       >  1:1664616/1‑150 (MQ=255)
                                                                                                                                  cAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACg                    <  1:1030587/150‑1 (MQ=255)
                                                                                                                                  cAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACg                    >  2:27582/1‑150 (MQ=255)
                                                                                                                                  cAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACATTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACg                    <  2:1453721/150‑1 (MQ=255)
                                                                                                                                      ggCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCa                >  2:945004/1‑150 (MQ=255)
                                                                                                                                        cgcgATCAGTTGATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGGTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAgg              <  1:1545342/150‑1 (MQ=255)
                                                                                                                                         gcgATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGa             <  2:1205445/150‑1 (MQ=255)
                                                                                                                                          cgATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGACCGCGGTTATCGCTACGATCAGGaa            >  1:391048/1‑150 (MQ=255)
                                                                                                                                          cgATCAGTTCAAAGAATTGTACACGGCTGATATCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCGCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGaa            <  1:618821/150‑1 (MQ=255)
                                                                                                                                           gATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAAt           >  1:623049/1‑150 (MQ=255)
                                                                                                                                           gATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAAt           <  2:921194/150‑1 (MQ=255)
                                                                                                                                               aGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAATGccc       >  2:1609657/1‑150 (MQ=255)
                                                                                                                                                gTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTTCGATCAGGAATGCCCg      >  1:1504458/1‑150 (MQ=255)
                                                                                                                                                   cATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCt   >  1:20109/1‑150 (MQ=255)
                                                                                                                                                   cATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGGCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGGTCACGCGGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCt   <  2:1540864/150‑1 (MQ=255)
                                                                                                                                                    aTTGAATTGTTCACGGCTGATTTAGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTg  >  2:669091/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTCGCCTACCCGTTCACGCACCAGCGGCACGCGAGTAGAACCGCCCACCATCACCACTTCCAGCACTTCATCAGCTTCTACACCCGCGTCTTTCAGCGCGCGACGACAAGCCAGTAAGGTTCGTTTTACCAGTGGCGCGATCAGTTCATTGAATTGTTCACGGCTGATTTCGCCCTGCCAGCCCGCAACGTTAACGGTCACGGAGTCCGCATCGCTCAGCGCGATTTTGGCTGCAATGGCGGCATCCAGCAGTTCACGCTGAACGCGGTTATCGCTACGATCAGGAATGCCCGCCTG  >  NC_000913/2657885‑2658182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: