Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,662,376 T→A 5.1% intergenic (‑245/+207) iscR ← / ← trmJ isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,662,3760TA 5.1% 329.2 / 2.9 110intergenic (‑245/+207)iscR/trmJisc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (47/57);  new base A (2/4);  total (49/61)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

TTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTA  >  NC_000913/2662228‑2662523
                                                                                                                                                    |                                                                                                                                                   
ttGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTa                                                                                                                                                    <  2:1184596/150‑1 (MQ=255)
ttGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTa                                                                                                                                                    <  2:1106014/150‑1 (MQ=255)
  ggTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGATCGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTAcg                                                                                                                                                  >  1:89449/1‑150 (MQ=255)
     cAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGt                                                                                                                                               >  2:1215202/1‑150 (MQ=255)
      aaCTATTTACTTGACTGATTTAGTCGGGTATTTATCCTTCAGTGGCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTc                                                                                                                                              <  2:703933/150‑1 (MQ=255)
      aaCTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGGGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTc                                                                                                                                              <  2:211963/150‑1 (MQ=255)
           ttaaCTTGAATGATTTAGGCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGGTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATc                                                                                                                                         <  2:1044817/147‑1 (MQ=255)
            ttACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCa                                                                                                                                        <  1:761194/150‑1 (MQ=255)
            ttACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCCGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCa                                                                                                                                        >  2:1482790/1‑150 (MQ=255)
             tACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAg                                                                                                                                       >  1:1448363/1‑150 (MQ=255)
              aCTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTTGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATGCGACGCGTAATGCGTCTTATCAgg                                                                                                                                      <  1:805539/150‑1 (MQ=37)
              aCTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAgg                                                                                                                                      <  2:1425697/150‑1 (MQ=255)
              aCTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAgg                                                                                                                                      <  1:419538/150‑1 (MQ=255)
              aCTTGACTGATTTAGGCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATGCGACGCGTAATGCGTCTTATCAgg                                                                                                                                      <  2:642308/150‑1 (MQ=37)
               cTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGc                                                                                                                                     >  2:551533/1‑150 (MQ=255)
                  gACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAAc                                                                                                                                  <  2:216266/150‑1 (MQ=255)
                  gACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAAc                                                                                                                                  <  1:551533/150‑1 (MQ=255)
                  gACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAAc                                                                                                                                  <  1:1496659/150‑1 (MQ=255)
                  gACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAAc                                                                                                                                  >  1:538445/1‑150 (MQ=255)
                  gACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAAc                                                                                                                                  >  1:1014799/1‑150 (MQ=255)
                   aCTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACg                                                                                                                                 <  2:193252/150‑1 (MQ=255)
                    cTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTTTACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACgg                                                                                                                                <  1:322291/150‑1 (MQ=255)
                      gATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATTAGACGCATTACGCGTCGCATCAGGCAACGGCt                                                                                                                              >  1:299852/1‑150 (MQ=255)
                      gATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCt                                                                                                                              <  2:1476566/150‑1 (MQ=255)
                       aTTTAGTCGGGTATTTACCCTTCTGTGCCATTTTTTTATCGTGGCGTTTGAACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTGCTGAACACTGAAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTg                                                                                                                             <  1:1284031/150‑1 (MQ=25)
                          tAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACAGTAGGCCTGATAAGACGCATTACGAGTCGCATCAGGCAACGGCTGTcc                                                                                                                          >  2:1070209/1‑149 (MQ=255)
                            gTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGa                                                                                                                        >  2:630468/1‑150 (MQ=255)
                            gTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGa                                                                                                                        >  1:389712/1‑150 (MQ=255)
                               gggTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATgc                                                                                                                     >  1:173986/1‑150 (MQ=255)
                                   aTTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATgcggcg                                                                                                                 >  1:138055/1‑150 (MQ=255)
                                     ttAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTg                                                                                                               >  1:120583/1‑150 (MQ=255)
                                      tAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACACCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGa                                                                                                              >  1:1400695/1‑150 (MQ=255)
                                         ccTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACg                                                                                                           >  2:958728/1‑150 (MQ=255)
                                          cTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGc                                                                                                          <  2:967050/150‑1 (MQ=255)
                                           ttCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGcc                                                                                                         >  1:470645/1‑150 (MQ=255)
                                                  ccATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCg                                                                                                  >  1:921684/1‑150 (MQ=255)
                                                  ccATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCg                                                                                                  >  1:642314/1‑150 (MQ=255)
                                                     tttttttATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCATGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGAcc                                                                                               <  1:1705821/150‑1 (MQ=255)
                                                          ttATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACgg                                                                                          <  2:644225/150‑1 (MQ=255)
                                                             tCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTGCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTc                                                                                       <  1:265406/150‑1 (MQ=255)
                                                               gTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTg                                                                                     <  2:944631/150‑1 (MQ=255)
                                                                  gCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTc                                                                                  <  2:386624/150‑1 (MQ=255)
                                                                  gCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTc                                                                                  <  1:630468/150‑1 (MQ=255)
                                                                  gCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTc                                                                                  <  2:591967/150‑1 (MQ=255)
                                                                    gTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCAc                                                                                <  1:476879/150‑1 (MQ=255)
                                                                    gTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCAc                                                                                <  2:1634954/150‑1 (MQ=255)
                                                                      ttGTACCGGTGGGCGGATGCGGCGTGACCGCCATATCCGCCCTGGGGTTCTGTCCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTg                                                                              <  1:1239039/150‑1 (MQ=14)
                                                                       tGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTAGGGTTCTGTTCACTGt                                                                             >  1:127014/1‑150 (MQ=255)
                                                                       tGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGt                                                                             <  2:581453/150‑1 (MQ=255)
                                                                       tGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGt                                                                             <  2:538445/150‑1 (MQ=255)
                                                                       tGTACCTGTTGGGGGATGAGGCTTGAACGCCATATCGGCCCTGGGGTTCTGTACAGTGTCGCCGTGATAAGACGCATTAGGCGTCGCTTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTGACTGt                                                                             <  2:1272880/150‑1 (MQ=14)
                                                                            cTGTTGTCTGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGTCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGcc                                                                        <  2:56619/150‑1 (MQ=255)
                                                                             tgttgtCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCt                                                                       >  2:1043835/1‑150 (MQ=255)
                                                                                tgtCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGAt                                                                    >  2:461114/1‑150 (MQ=255)
                                                                                 gtCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATa                                                                   <  1:1638448/150‑1 (MQ=255)
                                                                                 gtCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATa                                                                   <  1:1496907/150‑1 (MQ=255)
                                                                                 gtCGGATGCGGCGGGAACGCCATATCCGCCCGGGGGTTCTGTACACGGTAGGCCTGATAAGACGCATAACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATa                                                                   <  1:1564480/150‑1 (MQ=255)
                                                                                   cGGATATGGCGTTCACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAg                                                                 >  2:1212178/1‑150 (MQ=255)
                                                                                     gATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGAc                                                               <  2:642314/150‑1 (MQ=255)
                                                                                        gcggcgTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCa                                                            <  1:1391032/150‑1 (MQ=255)
                                                                                        gcggcgTGAACGCCATATCCGCCCTGGGGTTCTGAACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCa                                                            <  1:743760/150‑1 (MQ=255)
                                                                                          ggcgTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCAtt                                                          >  1:304551/1‑150 (MQ=255)
                                                                                          ggcgTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCAtt                                                          >  2:1710463/1‑150 (MQ=255)
                                                                                           gcgTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTa                                                         >  1:555307/1‑150 (MQ=255)
                                                                                           gcgTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCAGTa                                                         >  2:1589373/1‑150 (MQ=255)
                                                                                             gTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAcg                                                       <  1:414073/150‑1 (MQ=255)
                                                                                              tGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAcgc                                                      <  1:485801/150‑1 (MQ=255)
                                                                                               gAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTAcgcg                                                     <  2:768438/150‑1 (MQ=255)
                                                                                                 aCGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTc                                                   <  1:661529/150‑1 (MQ=255)
                                                                                                     cATATCCGCCCTGGGGTTCTGTACACTGTAGGACTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGGCTTATCCGACCTAAGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCAt                                               >  1:304293/1‑150 (MQ=255)
                                                                                                        atCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACATACGGTTCTGTTAACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAg                                            >  1:125625/1‑150 (MQ=255)
                                                                                                         tCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAgg                                           <  2:1400695/150‑1 (MQ=39)
                                                                                                         tCCGCCCTGGGGTTCTCTCCACTGTAGGCGTTATAAGACGCATTACGCGGCGCATCTGGAAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAgg                                           <  1:795585/150‑1 (MQ=11)
                                                                                                         tCCGCCCGGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATACCGCGTCGCAGCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAgg                                           <  1:1051673/150‑1 (MQ=255)
                                                                                                             cccTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTATTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAAc                                       <  2:172440/150‑1 (MQ=37)
                                                                                                             cccTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACAGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAAc                                       >  2:682422/1‑150 (MQ=37)
                                                                                                               cTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACgg                                     <  2:89449/150‑1 (MQ=35)
                                                                                                               cTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTGCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACgg                                     <  1:645268/150‑1 (MQ=25)
                                                                                                                tGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGc                                    <  2:921684/150‑1 (MQ=34)
                                                                                                                 ggggTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCt                                   <  2:389712/150‑1 (MQ=33)
                                                                                                                  gggTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGATCACTGTAGGCCTGAAAAGACGCATTACGCGTCGCATCAGGCAACGCCTg                                  >  1:410598/1‑150 (MQ=255)
                                                                                                                  gggTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTg                                  >  1:1326287/1‑150 (MQ=32)
                                                                                                                   ggTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGt                                 >  1:176642/1‑150 (MQ=32)
                                                                                                                    gTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTc                                >  1:1714751/1‑150 (MQ=32)
                                                                                                                    gTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTc                                >  2:14493/1‑150 (MQ=32)
                                                                                                                     ttCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCg                               >  2:453866/1‑150 (MQ=33)
                                                                                                                     gtCTGTACACTGTAGGCATGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCg                               <  1:1333455/149‑1 (MQ=18)
                                                                                                                             acTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATgcggc                       >  2:941368/1‑150 (MQ=38)
                                                                                                                                    gCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACg                >  1:1625622/1‑150 (MQ=255)
                                                                                                                                    gCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACg                >  2:627376/1‑150 (MQ=255)
                                                                                                                                     ccTGATGCGACGCGTAATGCGTCTTATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGc               >  2:77693/1‑150 (MQ=14)
                                                                                                                                      cTGATGCGACGCGTAATGCGTCTTATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCATGCAACGTCAGTCGGAATCGGCGTGAACGcc              >  2:331954/1‑150 (MQ=14)
                                                                                                                                      cTGATATGACGCATTTTCCGTCGCATCAGGCAACGGCTGTCGGATGCTGCGTTAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTTCTCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGcc              <  2:120583/150‑1 (MQ=14)
                                                                                                                                       tGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCt             <  2:470645/150‑1 (MQ=255)
                                                                                                                                       tGATAAGACGCATTACGCGTCGCAGCAGGCAACGGCGGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCAATGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCt             <  2:1114328/150‑1 (MQ=255)
                                                                                                                                        gATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCtt            <  1:1482790/150‑1 (MQ=255)
                                                                                                                                           aaGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATc         >  1:1391476/1‑150 (MQ=255)
                                                                                                                                               cGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGAc     <  2:248655/150‑1 (MQ=255)
                                                                                                                                               cGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGAc     >  2:1393168/1‑150 (MQ=255)
                                                                                                                                                  aTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa  >  1:1266834/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTA  >  NC_000913/2662228‑2662523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: