Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,672,640 G→T 6.6% Q198H (CAG→CAT hcaD → phenylpropionate dioxygenase, ferredoxin reductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,672,6400GT 6.6% 293.5 / 6.7 94Q198H (CAG→CAThcaDphenylpropionate dioxygenase, ferredoxin reductase subunit
Reads supporting (aligned to +/- strand):  ref base G (44/43);  new base T (5/2);  total (49/45)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 101 total aligned reads displayed.

TTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTCGCGA  >  NC_000913/2672492‑2672789
                                                                                                                                                     |                                                                                                                                                     
ttATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAgg                                                                                                                                                      >  2:1368903/1‑150 (MQ=255)
ttATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTCAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAgg                                                                                                                                                      <  1:975037/150‑1 (MQ=255)
     ggTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGgt                                                                                                                                                 >  2:160134/1‑150 (MQ=255)
      gTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGgtg                                                                                                                                                <  1:593070/150‑1 (MQ=255)
      gTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGgtg                                                                                                                                                >  2:94751/1‑150 (MQ=255)
      gTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGgtg                                                                                                                                                <  2:701665/150‑1 (MQ=255)
      gTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGgtg                                                                                                                                                <  2:614685/150‑1 (MQ=255)
        gCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTGAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCTGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGtgtg                                                                                                                                              <  2:386804/150‑1 (MQ=255)
        gCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGtgtg                                                                                                                                              <  2:1682996/150‑1 (MQ=255)
         ccGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTgc                                                                                                                                             >  1:149788/1‑150 (MQ=255)
          cGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGAGTgcg                                                                                                                                            >  2:376106/1‑150 (MQ=255)
            gAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCa                                                                                                                                          >  1:207349/1‑150 (MQ=255)
            gAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCa                                                                                                                                          <  2:701498/150‑1 (MQ=255)
              aCTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCACACCAGCCTGCTGgtggcgctgt                                                                                                                                         <  2:345498/150‑8 (MQ=255)
               cTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCCACGCTATCTTTTACCGCGCCACCAGCAGGCTGGTGTGCGCAttg                                                                                                                                       >  2:1076930/1‑149 (MQ=255)
                   tGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCt                                                                                                                                   <  2:1660280/150‑1 (MQ=255)
                    gggcTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTc                                                                                                                                  <  1:771722/147‑1 (MQ=255)
                     gTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCa                                                                                                                                 <  2:1345355/150‑1 (MQ=255)
                     gTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGAGACAGTGACTGAACTGGCGGCAACCGTCATGGGCCGTACTGCACCACCGCCCGTGCAACGCTTTCTTTTACAGCGCAACCCCCAGGCTGCGGTGCGCATTCTGCtaa                                                                                                                                 >  1:1045055/1‑148 (MQ=255)
                        tGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCaata                                                                                                                              <  2:1234910/150‑1 (MQ=255)
                            aCTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGc                                                                                                                          <  2:976929/150‑1 (MQ=255)
                                gCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGACGTAATGCACCACCGCACGTGCAACGCAAGCATTTACAGCGCCACCAGCATGCTGGTGTGCGCATTCTGCTCAATAAAGCCAtg                                                                                                                      >  2:1656788/1‑149 (MQ=255)
                                  tGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCGGCTCAATAATGCCATTGa                                                                                                                    >  2:1371940/1‑150 (MQ=255)
                                   gCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCAGTGaa                                                                                                                   >  1:1003157/1‑150 (MQ=255)
                                     cAGCGCCTCGCAGCGCAGATGGAAGGTGAGAGTGATTGAACTGGCCGCAACCGTCATGGGCCGTAATGCACCACCGCCGGTGCATCGCTATCTTTTACAGCGCCACCAGCATGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAAct                                                                                                                 <  2:291559/150‑2 (MQ=255)
                                      aGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACAt                                                                                                                <  1:1619880/150‑1 (MQ=255)
                                      aGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACAt                                                                                                                >  2:750186/1‑150 (MQ=255)
                                         gcCACGCAGCGCAGATGTGAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACAtgtg                                                                                                             <  2:1265034/150‑1 (MQ=255)
                                         gcCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACAtgtg                                                                                                             <  2:191583/150‑1 (MQ=255)
                                         gcCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACAtgtg                                                                                                             >  2:1035273/1‑150 (MQ=255)
                                           cACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGGTCAATAATGCCATTGATCATGTGGt                                                                                                           >  2:70156/1‑150 (MQ=255)
                                           cACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTg                                                                                                                     >  1:530611/1‑140 (MQ=255)
                                           cACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTg                                                                                                                     <  2:530611/140‑1 (MQ=255)
                                             cgcagcgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCg                                                                                                         >  2:186579/1‑150 (MQ=255)
                                                agcgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATg                                                                                                      >  1:1448294/1‑150 (MQ=255)
                                                       aTGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCTCCACCAGCAGGCTGTTGTGCGCATTCTGCTCAATAATGCCATCGACCACATGTTCAATggcattat                                                                                               <  2:344272/150‑7 (MQ=255)
                                                         gTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAg                                                                                             <  2:712361/150‑1 (MQ=255)
                                                          tAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGt                                                                                            >  1:38890/1‑150 (MQ=255)
                                                            aGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAg                                                                                          >  2:1413721/1‑150 (MQ=255)
                                                            aGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGAGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAg                                                                                          <  1:1267597/150‑1 (MQ=255)
                                                             ggTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGa                                                                                         <  1:1333472/150‑1 (MQ=255)
                                                                gACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACt                                                                                      <  1:379792/150‑1 (MQ=255)
                                                                  cAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGa                                                                                    >  1:207271/1‑150 (MQ=255)
                                                                    gTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACg                                                                                  <  2:789182/150‑1 (MQ=255)
                                                                       aTTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTg                                                                               <  2:1066413/150‑1 (MQ=255)
                                                                       aTTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTg                                                                               >  2:597933/1‑150 (MQ=255)
                                                                       aTTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTg                                                                               <  1:169847/150‑1 (MQ=255)
                                                                         tGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCa                                                                             >  2:646890/1‑150 (MQ=255)
                                                                             cTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGt                                                                         >  1:1312630/1‑150 (MQ=255)
                                                                             cTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGt                                                                         >  1:785483/1‑150 (MQ=255)
                                                                             cTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGt                                                                         >  1:792070/1‑150 (MQ=255)
                                                                               ggcggcAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTgg                                                                       <  1:806545/150‑1 (MQ=255)
                                                                                 cggcAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTgggg                                                                     <  1:94751/150‑1 (MQ=255)
                                                                                    catccGTCATGGGCCGTAATGCACCACCGCCCGTGGAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCTTTTAACATGTGGTGTATGGTGAATAAGTAGAACTGACGCTGCAAAGTGGGGata                                                                  <  2:527504/147‑3 (MQ=255)
                                                                                     aaCCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAAc                                                                 >  1:1549355/1‑150 (MQ=255)
                                                                                     aaCCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAAc                                                                 >  1:53688/1‑150 (MQ=255)
                                                                                     aaCCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAAc                                                                 <  2:149788/150‑1 (MQ=255)
                                                                                      aCCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGAGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACg                                                                <  1:376106/150‑1 (MQ=255)
                                                                                       ccGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGc                                                               <  1:505658/150‑1 (MQ=255)
                                                                                          tCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATACTGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGCCGCTACAAAGTGGGGAAACGCTTc                                                            <  1:1191774/150‑1 (MQ=255)
                                                                                           cATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCa                                                           >  2:1416807/1‑150 (MQ=255)
                                                                                           cATGGGCCGTAAAGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCAGCACAATAATGCCTTTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTCCAAAGTGGGGAAACGCTTCa                                                           >  2:405566/1‑150 (MQ=255)
                                                                                             tGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAgg                                                         >  2:450135/1‑150 (MQ=255)
                                                                                                gCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTg                                                      >  1:1402669/1‑150 (MQ=255)
                                                                                                gCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTg                                                      >  2:262616/1‑150 (MQ=255)
                                                                                                   gTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGAtg                                                   >  2:386984/1‑150 (MQ=255)
                                                                                                    tAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGACTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGAtgt                                                  >  2:1517237/1‑150 (MQ=255)
                                                                                                      aTGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtgg                                                >  2:739323/1‑150 (MQ=255)
                                                                                                      aTGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTCAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtgg                                                >  1:319293/1‑150 (MQ=255)
                                                                                                       tGCACCACCGCCCGTGCATCGCTATCTTTTACAGCGCCACCCGCAGGCTGGTGTGCGCATTCAGCTCAATACTGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtggt                                               <  1:252588/150‑1 (MQ=255)
                                                                                                          accaccGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGAt                                            <  2:1428452/150‑1 (MQ=255)
                                                                                                           ccaccGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGAtt                                           >  2:1608934/1‑150 (MQ=255)
                                                                                                            caccGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGAttt                                          >  1:1498267/1‑150 (MQ=255)
                                                                                                               cGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACg                                       <  2:1258928/150‑1 (MQ=255)
                                                                                                               cGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACg                                       <  1:927143/150‑1 (MQ=255)
                                                                                                                gCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACgg                                      <  1:160134/150‑1 (MQ=255)
                                                                                                                 cccGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGt                                     <  2:1459750/150‑1 (MQ=255)
                                                                                                                  ccGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTa                                    >  1:1232765/1‑150 (MQ=255)
                                                                                                                   cGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTAt                                   >  1:640680/1‑150 (MQ=255)
                                                                                                                      gCATCGCTTTCTTTTACAGCGCCACCAGCAGGCTGGGGTGCGCATTCTGCTCAATAATGCCCTTGAACATGTGGTCGAGGGGGAAACAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTTTTgg                                <  2:17959/150‑1 (MQ=255)
                                                                                                                      gCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTgg                                <  2:1189237/150‑1 (MQ=255)
                                                                                                                        aaCGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTa                              <  2:430037/150‑1 (MQ=255)
                                                                                                                          cGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATc                            <  2:207271/150‑1 (MQ=255)
                                                                                                                            cTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAg                          >  1:1189236/1‑150 (MQ=255)
                                                                                                                             tATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAgc                         >  2:1085378/1‑150 (MQ=255)
                                                                                                                              aTCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAgcg                        <  2:1362301/150‑1 (MQ=255)
                                                                                                                                cTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGcc                      >  2:424395/1‑150 (MQ=255)
                                                                                                                                    tACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACg                  <  1:1386871/150‑1 (MQ=255)
                                                                                                                                       aGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGc               >  2:468946/1‑150 (MQ=255)
                                                                                                                                       aGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGc               <  2:1448294/150‑1 (MQ=255)
                                                                                                                                         cgcacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAAAGCCATTGAACAAGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAAAGAGCa              >  1:144970/5‑150 (MQ=255)
                                                                                                                                           cacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAAc            >  1:1040482/3‑150 (MQ=255)
                                                                                                                                           cacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAAc            >  2:307597/3‑150 (MQ=255)
                                                                                                                                           cacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAAc            >  1:1398139/3‑150 (MQ=255)
                                                                                                                                             caccaGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTg          >  1:43154/1‑150 (MQ=255)
                                                                                                                                                cagcagGCTGGTGTGCGCATTCTGCTCAATACTTCCATTGAACATGTGGTCGATGGGGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTAGCAGCGCCAACGAGCAACTGGCt       <  1:270134/150‑1 (MQ=255)
                                                                                                                                                   cagGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTcgc    >  1:1074304/1‑150 (MQ=255)
                                                                                                                                                     ggCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTCGCGa  >  2:651099/1‑150 (MQ=255)
                                                                                                                                                     ggCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTCGCGa  >  2:1352346/1‑150 (MQ=255)
                                                                                                                                                     ggCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTCGCGa  >  2:1059506/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTATCGGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTCGCGA  >  NC_000913/2672492‑2672789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: