Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,687,044 A→T 6.8% intergenic (+19/+26) hmp → / ← glnB fused nitric oxide dioxygenase/dihydropteridine reductase 2/regulatory protein P‑II for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,687,0440AT 6.8% 296.4 / 7.2 101intergenic (+19/+26)hmp/glnBfused nitric oxide dioxygenase/dihydropteridine reductase 2/regulatory protein P‑II for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base A (45/49);  new base T (5/2);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

ATCCGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAG  >  NC_000913/2686895‑2687193
                                                                                                                                                     |                                                                                                                                                     
aTCCGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGa                                                                                                                                                       <  2:1523508/150‑1 (MQ=255)
 tCCGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGaa                                                                                                                                                      >  1:1524354/1‑150 (MQ=255)
   cGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGAGCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAAc                                                                                                                                                    <  2:1696981/150‑1 (MQ=255)
       aaTGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATcc                                                                                                                                                >  1:596419/1‑150 (MQ=255)
        aTGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGCGAAGCAGGAAAACCTTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATTTTGCCGGATGGTAACATCCg                                                                                                                                               >  1:97428/1‑150 (MQ=255)
        aTGCAGTTCTATCTCTGCGGCACTGTTGGCTTCATGCAGTTTACCGCGAAAAAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCACGCATAAGGTGCTGTAATTTGATGTTGCAGGATGGAAACATCCg                                                                                                                                               >  1:57657/1‑150 (MQ=255)
         tGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCgg                                                                                                                                              <  2:794070/150‑1 (MQ=255)
         tGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCgg                                                                                                                                              <  1:175406/150‑1 (MQ=255)
         tGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCgg                                                                                                                                              <  2:412788/150‑1 (MQ=255)
          gCAGTTCTATCTCTGCGGCCCGTTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGGGAAGCAGGAAAACATTCATTACGACTGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGc                                                                                                                                             <  1:1506505/150‑1 (MQ=255)
             gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGTTTCCATCCGGCAAc                                                                                                                                          <  2:1320540/150‑1 (MQ=255)
             gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGTTTCCATCCGGCAAc                                                                                                                                          <  2:1313659/150‑1 (MQ=255)
             gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAACCATCCGGCAAc                                                                                                                                          >  2:1552217/1‑150 (MQ=255)
             gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAAc                                                                                                                                          <  1:179652/150‑1 (MQ=255)
             gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAAc                                                                                                                                          >  2:134619/1‑150 (MQ=255)
              ttCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCTCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAAcc                                                                                                                                         <  1:112750/150‑1 (MQ=255)
                  aTCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTg                                                                                                                                     >  1:1329524/1‑150 (MQ=255)
                      ctGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGAcgc                                                                                                                                 >  2:1534641/1‑150 (MQ=255)
                        gCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCgg                                                                                                                               <  2:982567/150‑1 (MQ=255)
                         cGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATACGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGt                                                                                                                              <  1:1512702/150‑1 (MQ=255)
                         cGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTGGCCGGATGGAAACATCCGGCAACCCTTGACGCGGt                                                                                                                              <  1:575665/150‑1 (MQ=255)
                            cccGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTTGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTa                                                                                                                           <  2:596419/150‑1 (MQ=255)
                            cccGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTa                                                                                                                           <  1:545/150‑1 (MQ=255)
                              cGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCACGCATAAGGTGCTGTAATTTGATTTTGCCGGATGGAAAAATCAGGCAACCCTTGACGCGGTATaaa                                                                                                                         >  2:1481462/1‑150 (MQ=255)
                              cGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGGGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTaaa                                                                                                                         <  2:75183/150‑1 (MQ=255)
                               ggTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAAt                                                                                                                        >  1:938607/1‑150 (MQ=255)
                                    gCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACTAATGCTTTGGCCCGCATAAGGTGCTCTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGccc                                                                                                                   >  2:1439480/1‑149 (MQ=255)
                                    gCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCcg                                                                                                                   <  1:1260898/150‑1 (MQ=255)
                                    gCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCcg                                                                                                                   >  1:544031/1‑150 (MQ=255)
                                    gCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGAAACCCTTGACGCGGTTTAAATTGCcg                                                                                                                   >  2:647258/1‑150 (MQ=255)
                                        cATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGcgta                                                                                                               >  1:1148755/1‑149 (MQ=255)
                                          tGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGcgtcgt                                                                                                             >  1:92501/1‑150 (MQ=255)
                                           gCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCgtcgtc                                                                                                            >  1:1148747/1‑150 (MQ=255)
                                               tttACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCctct                                                                                                        <  2:544031/150‑1 (MQ=255)
                                                   ccGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCAc                                                                                                    <  1:500486/150‑1 (MQ=255)
                                                   ccGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTACACGCGGTTTAAATTGCCGCGTCGTCCTCTTCAc                                                                                                    >  2:1439334/1‑150 (MQ=255)
                                                    cgctAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGAAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCAcc                                                                                                   <  2:847953/150‑1 (MQ=255)
                                                    cgcgAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCAcc                                                                                                   <  2:1293167/150‑1 (MQ=255)
                                                     gcgAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCg                                                                                                  <  1:1436924/150‑1 (MQ=255)
                                                       gAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGt                                                                                                <  2:1148747/150‑1 (MQ=255)
                                                         aaCAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAAAGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTAc                                                                                              <  1:1119365/150‑1 (MQ=255)
                                                         aaCACTTAGTGGAGCTGGGCGCGACGCAGGAAAACATTCACTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTAc                                                                                              <  1:275786/150‑1 (MQ=255)
                                                          aCAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACg                                                                                             >  1:1434885/1‑150 (MQ=255)
                                                          aCAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACg                                                                                             >  2:239457/1‑150 (MQ=255)
                                                              ttAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTcc                                                                                                           >  1:142826/1‑132 (MQ=255)
                                                              ttAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTcc                                                                                                           <  2:142826/132‑1 (MQ=255)
                                                              ttAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTcc                                                                                                           >  1:187327/1‑132 (MQ=255)
                                                               tAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGGTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGc                                                                                        <  2:1333530/150‑1 (MQ=255)
                                                                 gTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGa                                                                                      >  2:845771/1‑150 (MQ=255)
                                                                  ttgttCTGGGCGTGAAGCATGAAAAATTTAATTACGTTTGCTTTGGACCGCATAAGGTGCTGTAATTTGATTTTGACGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTGACCGGTACTGATGCTaa                                                                                     <  2:786288/146‑1 (MQ=255)
                                                                    gATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATg                                                                                   >  1:1226433/1‑150 (MQ=255)
                                                                    gATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATg                                                                                   <  2:602250/150‑1 (MQ=255)
                                                                      tCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTAGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGAc                                                                                 <  1:114800/150‑1 (MQ=255)
                                                                        tGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGAccc                                                                               <  2:57657/150‑1 (MQ=255)
                                                                         gggCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCg                                                                              >  1:95444/1‑150 (MQ=255)
                                                                           gCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTg                                                                            >  1:1336693/1‑150 (MQ=255)
                                                                             gTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGcc                                                                          >  2:23584/1‑150 (MQ=255)
                                                                                aaGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACg                                                                       >  2:513832/1‑150 (MQ=255)
                                                                                 aGCAGGAAAACATTCATTACGTCTGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGTCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGt                                                                      <  2:97428/150‑1 (MQ=255)
                                                                                 aGCAGGAAAAAATTCATTAAGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGt                                                                      >  2:916572/1‑150 (MQ=255)
                                                                                  gCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTc                                                                     <  1:1220678/150‑1 (MQ=255)
                                                                                  gCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTc                                                                     >  2:1673670/1‑150 (MQ=255)
                                                                                     ggAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGTATGACCCGTGCCACGTCaaa                                                                  >  2:743104/1‑150 (MQ=255)
                                                                                      gAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAg                                                                 <  1:134619/150‑1 (MQ=255)
                                                                                       aaaaCATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAgg                                                                >  1:387317/1‑149 (MQ=255)
                                                                                        aaaCATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGAc                                                               >  2:587437/1‑150 (MQ=255)
                                                                                          aCATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGa                                                             <  2:92501/150‑1 (MQ=255)
                                                                                           cattcattACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGaa                                                            <  1:160646/150‑1 (MQ=255)
                                                                                                  tACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTa                                                     <  1:845771/150‑1 (MQ=255)
                                                                                                  tACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTa                                                     >  2:1482849/1‑150 (MQ=255)
                                                                                                   aCGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTAc                                                    >  1:593137/1‑150 (MQ=255)
                                                                                                       aTGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTc                                                <  1:1007524/150‑1 (MQ=255)
                                                                                                       aTGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGGTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTc                                                <  1:1001021/150‑1 (MQ=255)
                                                                                                       aTGCTTTGGCCCGCATAAGGTGCGGTAATTTGATGTTGCCGGAGGGAAACATCCGGCAACCCTTGCCGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCggc                                                <  1:1007525/150‑3 (MQ=255)
                                                                                                        tGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCa                                               >  1:165967/1‑150 (MQ=255)
                                                                                                           tttGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCg                                            >  2:1656367/1‑150 (MQ=255)
                                                                                                             tGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGATACATCCGGCAACCCTTGACGCGGTTTAAATTGACGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTAACCGTCACCGAt                                          >  1:857079/1‑150 (MQ=255)
                                                                                                              ggCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTTCCACGTCAAAGACGAAGATTTTACCGTCACCGAtt                                         >  2:311240/1‑150 (MQ=255)
                                                                                                               gCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGAttt                                        >  2:1463311/1‑150 (MQ=255)
                                                                                                                 ccGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTg                                      >  1:1119718/1‑150 (MQ=255)
                                                                                                                 ccGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTg                                      <  1:189995/150‑1 (MQ=255)
                                                                                                                   gCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGcc                                    <  1:412297/150‑1 (MQ=255)
                                                                                                                     aTAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCgg                                  <  2:165967/150‑1 (MQ=255)
                                                                                                                      tAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACTCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTCCCACGTCAAAGCCGAAGATTTTACCGTCACCGATTTTGCCGGt                                 >  2:1508421/1‑150 (MQ=255)
                                                                                                                            gCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTgcgc                           >  2:1188501/1‑150 (MQ=255)
                                                                                                                                      gATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaata                 <  2:1329524/150‑1 (MQ=255)
                                                                                                                                        tGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaataat               <  1:1455324/150‑1 (MQ=255)
                                                                                                                                         gTTGCCGGATGTTTCCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCTAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATg              >  1:1097067/1‑150 (MQ=255)
                                                                                                                                         gTTGCCGGATGTTTCCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGAAGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATg              >  2:1405013/1‑150 (MQ=255)
                                                                                                                                           tGCCGGATGTTTCCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGt            >  2:1528608/1‑150 (MQ=255)
                                                                                                                                           tGCCGGATGTTTCCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGt            >  1:1472742/1‑150 (MQ=255)
                                                                                                                                            gCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGtt           <  1:23584/150‑2 (MQ=255)
                                                                                                                                            gCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTa           >  1:970540/1‑150 (MQ=255)
                                                                                                                                            gCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTAt          <  1:377646/150‑1 (MQ=255)
                                                                                                                                             ccGGATGGAAACATCCGTCAACCCTTGACGAGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAACACGAAGATTGTACCGTCTCCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTAt          <  2:282665/150‑1 (MQ=255)
                                                                                                                                             ccGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTAt          >  1:1070636/1‑150 (MQ=255)
                                                                                                                                                 aTGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGac      <  2:938607/150‑1 (MQ=255)
                                                                                                                                                  tGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGACACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGaca     >  2:266635/1‑150 (MQ=255)
                                                                                                                                                   ggAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGacac    <  2:95444/150‑1 (MQ=255)
                                                                                                                                                     aaaCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAg  <  2:1171367/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCCGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAG  >  NC_000913/2686895‑2687193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: