Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,687,046 A→C 6.7% intergenic (+21/+24) hmp → / ← glnB fused nitric oxide dioxygenase/dihydropteridine reductase 2/regulatory protein P‑II for glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,687,0460AC 6.7% 278.2 / 9.4 101intergenic (+21/+24)hmp/glnBfused nitric oxide dioxygenase/dihydropteridine reductase 2/regulatory protein P‑II for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base A (45/49);  new base C (5/2);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

Only 100 of 106 total aligned reads displayed.

CGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGG  >  NC_000913/2686898‑2687194
                                                                                                                                                    |                                                                                                                                                    
cGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGAGCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAAc                                                                                                                                                     <  2:1696981/150‑1 (MQ=255)
    aaTGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATcc                                                                                                                                                 >  1:596419/1‑150 (MQ=255)
     aTGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGCGAAGCAGGAAAACCTTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATTTTGCCGGATGGTAACATCCg                                                                                                                                                >  1:97428/1‑150 (MQ=255)
     aTGCAGTTCTATCTCTGCGGCACTGTTGGCTTCATGCAGTTTACCGCGAAAAAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCACGCATAAGGTGCTGTAATTTGATGTTGCAGGATGGAAACATCCg                                                                                                                                                >  1:57657/1‑150 (MQ=255)
      tGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCgg                                                                                                                                               <  2:794070/150‑1 (MQ=255)
      tGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCgg                                                                                                                                               <  1:175406/150‑1 (MQ=255)
      tGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCgg                                                                                                                                               <  2:412788/150‑1 (MQ=255)
       gCAGTTCTATCTCTGCGGCCCGTTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGGGAAGCAGGAAAACATTCATTACGACTGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGc                                                                                                                                              <  1:1506505/150‑1 (MQ=255)
          gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGTTTCCATCCGGCAAc                                                                                                                                           <  2:1313659/150‑1 (MQ=255)
          gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGTTTCCATCCGGCAAc                                                                                                                                           <  2:1320540/150‑1 (MQ=255)
          gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAACCATCCGGCAAc                                                                                                                                           >  2:1552217/1‑150 (MQ=255)
          gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAAc                                                                                                                                           >  2:134619/1‑150 (MQ=255)
          gTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAAc                                                                                                                                           <  1:179652/150‑1 (MQ=255)
           ttCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCTCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAAcc                                                                                                                                          <  1:112750/150‑1 (MQ=255)
               ttctcTGCGGCCCGGTGGGCTTCATGCAGTTTACCGCGAAACAGTTCTGGGATCGGGGCGGGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGGGCGGTAATTTGATGTGGCCGGATGGAAACATCCGGCAACCCTTg                                                                                                                                      <  1:1551302/149‑1 (MQ=255)
               aTCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTg                                                                                                                                      >  1:1329524/1‑150 (MQ=255)
                   ctGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGAcgc                                                                                                                                  >  2:1534641/1‑150 (MQ=255)
                     gCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCgg                                                                                                                                <  2:982567/150‑1 (MQ=255)
                      cGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATACGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGt                                                                                                                               <  1:1512702/150‑1 (MQ=255)
                      cGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTGGCCGGATGGAAACATCCGGCAACCCTTGACGCGGt                                                                                                                               <  1:575665/150‑1 (MQ=255)
                         cccGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTTGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTa                                                                                                                            <  2:596419/150‑1 (MQ=255)
                         cccGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTa                                                                                                                            <  1:545/150‑1 (MQ=255)
                           cGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCACGCATAAGGTGCTGTAATTTGATTTTGCCGGATGGAAAAATCAGGCAACCCTTGACGCGGTATaaa                                                                                                                          >  2:1481462/1‑150 (MQ=255)
                           cGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGGGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTaaa                                                                                                                          <  2:75183/150‑1 (MQ=255)
                            ggTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAAt                                                                                                                         >  1:938607/1‑150 (MQ=255)
                                 gCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACTAATGCTTTGGCCCGCATAAGGTGCTCTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGccc                                                                                                                    >  2:1439480/1‑149 (MQ=255)
                                 gCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCcg                                                                                                                    <  1:1260898/150‑1 (MQ=255)
                                 gCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCcg                                                                                                                    >  1:544031/1‑150 (MQ=255)
                                 gCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGAAACCCTTGACGCGGTTTAAATTGCcg                                                                                                                    >  2:647258/1‑150 (MQ=255)
                                     cATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGcgta                                                                                                                >  1:1148755/1‑149 (MQ=255)
                                       tGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGcgtcgt                                                                                                              >  1:92501/1‑150 (MQ=255)
                                        gCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCgtcgtc                                                                                                             >  1:1148747/1‑150 (MQ=255)
                                            tttACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCctct                                                                                                         <  2:544031/150‑1 (MQ=255)
                                                ccGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCAc                                                                                                     <  1:500486/150‑1 (MQ=255)
                                                ccGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTACACGCGGTTTAAATTGCCGCGTCGTCCTCTTCAc                                                                                                     >  2:1439334/1‑150 (MQ=255)
                                                 cgctAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGAAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCAcc                                                                                                    <  2:847953/150‑1 (MQ=255)
                                                 cgcgAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCAcc                                                                                                    <  2:1293167/150‑1 (MQ=255)
                                                  gcgAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCg                                                                                                   <  1:1436924/150‑1 (MQ=255)
                                                    gAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGt                                                                                                 <  2:1148747/150‑1 (MQ=255)
                                                      aaCAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAAAGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTAc                                                                                               <  1:1119365/150‑1 (MQ=255)
                                                      aaCACTTAGTGGAGCTGGGCGCGACGCAGGAAAACATTCACTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTAc                                                                                               <  1:275786/150‑1 (MQ=255)
                                                       aCAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACg                                                                                              >  2:239457/1‑150 (MQ=255)
                                                       aCAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACg                                                                                              >  1:1434885/1‑150 (MQ=255)
                                                           ttAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTcc                                                                                                            >  1:187327/1‑132 (MQ=255)
                                                           ttAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTcc                                                                                                            <  2:187327/132‑1 (MQ=255)
                                                           ttAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTcc                                                                                                            <  2:142826/132‑1 (MQ=255)
                                                            tAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGGTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGc                                                                                         <  2:1333530/150‑1 (MQ=255)
                                                              gTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGa                                                                                       >  2:845771/1‑150 (MQ=255)
                                                               ttgttCTGGGCGTGAAGCATGAAAAATTTAATTACGTTTGCTTTGGACCGCATAAGGTGCTGTAATTTGATTTTGACGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTGACCGGTACTGATGCTaa                                                                                      <  2:786288/146‑1 (MQ=255)
                                                                 gATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATg                                                                                    >  1:1226433/1‑150 (MQ=255)
                                                                 gATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATg                                                                                    <  2:602250/150‑1 (MQ=255)
                                                                   tCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTAGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGAc                                                                                  <  1:114800/150‑1 (MQ=255)
                                                                     tGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGAccc                                                                                <  2:57657/150‑1 (MQ=255)
                                                                      gggCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCg                                                                               >  1:95444/1‑150 (MQ=255)
                                                                        gCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTg                                                                             >  1:1336693/1‑150 (MQ=255)
                                                                          gTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGcc                                                                           >  2:23584/1‑150 (MQ=255)
                                                                             aaGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACg                                                                        >  2:513832/1‑150 (MQ=255)
                                                                              aGCAGGAAAACATTCATTACGTCTGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGTCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGt                                                                       <  2:97428/150‑1 (MQ=255)
                                                                              aGCAGGAAAAAATTCATTAAGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGt                                                                       >  2:916572/1‑150 (MQ=255)
                                                                               gCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTc                                                                      <  1:1220678/150‑1 (MQ=255)
                                                                               gCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTc                                                                      >  2:1673670/1‑150 (MQ=255)
                                                                                  ggAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGTATGACCCGTGCCACGTCaaa                                                                   >  2:743104/1‑150 (MQ=255)
                                                                                   gAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAg                                                                  <  1:134619/150‑1 (MQ=255)
                                                                                       aCATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGa                                                              <  2:92501/150‑1 (MQ=255)
                                                                                        cattcattACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGaa                                                             <  1:160646/150‑1 (MQ=255)
                                                                                               tACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTa                                                      <  1:845771/150‑1 (MQ=255)
                                                                                               tACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTa                                                      >  2:1482849/1‑150 (MQ=255)
                                                                                                aCGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTAc                                                     >  1:593137/1‑150 (MQ=255)
                                                                                                    aTGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTc                                                 <  1:1007524/150‑1 (MQ=255)
                                                                                                    aTGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGGTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTc                                                 <  1:1001021/150‑1 (MQ=255)
                                                                                                    aTGCTTTGGCCCGCATAAGGTGCGGTAATTTGATGTTGCCGGAGGGAAACATCCGGCAACCCTTGCCGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCggc                                                 <  1:1007525/150‑3 (MQ=255)
                                                                                                     tGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCa                                                >  1:165967/1‑150 (MQ=255)
                                                                                                        tttGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCg                                             >  2:1656367/1‑150 (MQ=255)
                                                                                                          tGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGATACATCCGGCAACCCTTGACGCGGTTTAAATTGACGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTAACCGTCACCGAt                                           >  1:857079/1‑150 (MQ=255)
                                                                                                           ggCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTTCCACGTCAAAGACGAAGATTTTACCGTCACCGAtt                                          >  2:311240/1‑150 (MQ=255)
                                                                                                            gCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGAttt                                         >  2:1463311/1‑150 (MQ=255)
                                                                                                              ccGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTg                                       >  1:1119718/1‑150 (MQ=255)
                                                                                                              ccGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTg                                       <  1:189995/150‑1 (MQ=255)
                                                                                                                gCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGcc                                     <  1:412297/150‑1 (MQ=255)
                                                                                                                  aTAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCgg                                   <  2:165967/150‑1 (MQ=255)
                                                                                                                         gCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTgcgc                            >  2:1188501/1‑150 (MQ=255)
                                                                                                                                   gATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaata                  <  2:1329524/150‑1 (MQ=255)
                                                                                                                                     tGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGaataat                <  1:1455324/150‑1 (MQ=255)
                                                                                                                                      gTTGCCGGATGTTTCCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCTAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATg               >  1:1097067/1‑150 (MQ=255)
                                                                                                                                      gTTGCCGGATGTTTCCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGAAGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATg               >  2:1405013/1‑150 (MQ=255)
                                                                                                                                        tGCCGGATGTTTCCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGt             >  2:1528608/1‑150 (MQ=255)
                                                                                                                                        tGCCGGATGTTTCCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGt             >  1:1472742/1‑150 (MQ=255)
                                                                                                                                         gCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGtt            <  1:23584/150‑2 (MQ=255)
                                                                                                                                         gCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTa            >  1:970540/1‑150 (MQ=255)
                                                                                                                                         gCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTAt           <  1:377646/150‑1 (MQ=255)
                                                                                                                                          ccGGATGGAAACATCCGTCAACCCTTGACGAGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAACACGAAGATTGTACCGTCTCCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTAt           <  2:282665/150‑1 (MQ=255)
                                                                                                                                          ccGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTAt           >  1:1070636/1‑150 (MQ=255)
                                                                                                                                            atccggcaaCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCg         >  2:923345/8‑150 (MQ=255)
                                                                                                                                              aTGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGac       <  2:938607/150‑1 (MQ=255)
                                                                                                                                               tGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGACACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGaca      >  2:266635/1‑150 (MQ=255)
                                                                                                                                                ggAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGacac     <  2:95444/150‑1 (MQ=255)
                                                                                                                                                  aaaCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAg   <  2:1171367/150‑1 (MQ=255)
                                                                                                                                                   aaCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAgg  <  1:239457/150‑1 (MQ=255)
                                                                                                                                                   aaCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAgg  >  1:685788/1‑150 (MQ=255)
                                                                                                                                                   aaCATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAgg  <  1:986334/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGACAATGCAGTTCTATCTCTGCGGCCCGGTTGGCTTCATGCAGTTTACCGCGAAACAGTTAGTGGATCTGGGCGTGAAGCAGGAAAACATTCATTACGAATGCTTTGGCCCGCATAAGGTGCTGTAATTTGATGTTGCCGGATGGAAACATCCGGCAACCCTTGACGCGGTTTAAATTGCCGCGTCGTCCTCTTCACCGGTACGGATGCGAATGACCCGTGCCACGTCAAAGACGAAGATTTTACCGTCACCGATTTTGCCGGTTTGCGCCGTGCGAATAATGGTATCGACACAGG  >  NC_000913/2686898‑2687194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: