Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,701,548 A→T 5.5% L61Q (CTG→CAG)  pdxJ ← pyridoxine 5'‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,701,5480AT 5.5% 339.3 / 3.7 112L61Q (CTG→CAG) pdxJpyridoxine 5'‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (46/59);  new base T (3/4);  total (49/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCGTAGCGATATGG  >  NC_000913/2701399‑2701697
                                                                                                                                                     |                                                                                                                                                     
cTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCa                                                                                                                                                       >  1:1427263/1‑150 (MQ=255)
 tGCGACATCCAAGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAg                                                                                                                                                      <  2:708152/150‑1 (MQ=255)
  gCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCGCCGGCGATCGCCCGCATCTCTTCGGTCCCCGCCATCTCCAGATTCCTGCGGGTATCCCGCGTCTGACGCAgg                                                                                                                                                     >  1:298995/1‑150 (MQ=255)
      cATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATgc                                                                                                                                                 <  2:802300/150‑1 (MQ=255)
          cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCAc                                                                                                                                             >  2:48985/1‑150 (MQ=255)
          cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCAc                                                                                                                                             <  1:1451446/150‑1 (MQ=255)
          cAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCAc                                                                                                                                             <  1:103374/150‑1 (MQ=255)
             gccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTc                                                                                                                                          <  1:883221/150‑1 (MQ=255)
             gccgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCCCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTc                                                                                                                                          >  2:194383/1‑150 (MQ=255)
               cgccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgc                                                                                                                                        >  1:309449/1‑150 (MQ=255)
                gccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTATCAACGGCGATCGCCAGCATCTCTACGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                                                                                                                       <  2:1251086/150‑1 (MQ=255)
                gccTGCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTcgcg                                                                                                                                       <  1:1107192/150‑1 (MQ=255)
                 ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCgg                                                                                                                                      <  2:1235262/150‑1 (MQ=255)
                 ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCgg                                                                                                                                      >  1:1272819/1‑150 (MQ=255)
                 ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCgg                                                                                                                                      <  2:1373833/150‑1 (MQ=255)
                 ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCgg                                                                                                                                      <  2:1477686/150‑1 (MQ=255)
                 ccTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCgg                                                                                                                                      <  1:1660509/150‑1 (MQ=255)
                     cGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAg                                                                                                                                  >  1:389246/1‑150 (MQ=255)
                      ggTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGt                                                                                                                                 >  1:193122/1‑150 (MQ=255)
                         tgttACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCCGTCacatta                                                                                                                              >  1:590381/1‑145 (MQ=255)
                            tACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtgtg                                                                                                                           <  1:876729/150‑1 (MQ=255)
                            tACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAAtgtg                                                                                                                           <  2:14399/150‑1 (MQ=255)
                              cTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGAc                                                                                                                         >  2:346430/1‑150 (MQ=255)
                              cTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGAc                                                                                                                         >  1:956494/1‑150 (MQ=255)
                                tCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACgg                                                                                                                       <  2:667650/150‑1 (MQ=255)
                                  cTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGACTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCg                                                                                                                     >  1:490211/1‑150 (MQ=255)
                                    gACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATTCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGAt                                                                                                                   >  2:407204/1‑150 (MQ=255)
                                     tcGCTTTTCCGGTACCAGGCAGGAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTGGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGGGTCTGACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATc                                                                                                                  <  1:417102/149‑1 (MQ=255)
                                        cTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTc                                                                                                               >  2:1532147/1‑150 (MQ=255)
                                         ttttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCa                                                                                                              <  1:1532147/150‑1 (MQ=255)
                                         ttttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCAATCTTCa                                                                                                              <  1:1150223/150‑1 (MQ=255)
                                           ttCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACg                                                                                                            <  2:176712/150‑1 (MQ=255)
                                                gTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGAACGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAAt                                                                                                       <  2:372462/150‑1 (MQ=255)
                                                     aGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACg                                                                                                  <  2:346550/150‑1 (MQ=255)
                                                      ggCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACgg                                                                                                 >  2:770908/1‑150 (MQ=255)
                                                          gcaAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAAt                                                                                             >  1:1456015/1‑150 (MQ=255)
                                                           caAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATg                                                                                            <  2:230144/150‑1 (MQ=255)
                                                                aTGTGGCTTCGTCTCAACGGCGATCGCCAGGATCTCTTCGGTCACCGCCATCTGCAGATTCATGCGGGTATCCAGCGTCTGTCGCAGGATGCGCTCGTCGAGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTc                                                                                       <  1:1514782/150‑1 (MQ=255)
                                                                aTGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTc                                                                                       <  1:1642220/150‑1 (MQ=255)
                                                                   tgGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGAGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGAAATGCCGTCCGc                                                                                    >  2:249238/1‑150 (MQ=255)
                                                                      cTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCTATCTTCACGTAAATGCACGGTAATGCCGTCCGCTcc                                                                                 <  1:995315/150‑1 (MQ=255)
                                                                       ttCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTccc                                                                                <  1:1026603/150‑1 (MQ=255)
                                                                         cGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTTACGCAGGATCCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGc                                                                              >  1:868269/1‑150 (MQ=255)
                                                                          gTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGcc                                                                             >  1:711420/1‑150 (MQ=255)
                                                                             tcAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGc                                                                          <  2:1272819/150‑1 (MQ=255)
                                                                               aaCGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTc                                                                        <  2:1208673/150‑1 (MQ=255)
                                                                               aaCGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTc                                                                        <  2:1186237/150‑1 (MQ=255)
                                                                                aCGGCGATCGCCAGCATCTCTTCGGTAACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCg                                                                       >  1:1498371/1‑150 (MQ=255)
                                                                                 cGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCgg                                                                      >  2:687874/1‑150 (MQ=255)
                                                                                  ggCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGc                                                                     <  2:1578234/150‑1 (MQ=255)
                                                                                  ggCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGc                                                                     <  1:778592/150‑1 (MQ=255)
                                                                                  ggCGAGCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCTGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGc                                                                     <  2:267213/150‑1 (MQ=255)
                                                                                     gATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAAt                                                                  <  2:193122/150‑1 (MQ=255)
                                                                                       tCGCCAGCATCTCTTCGGTCACCGCCATCACCAGAATCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATaa                                                                <  1:856629/150‑1 (MQ=255)
                                                                                        cGCCAGCATCTCTTCGGGCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATaaa                                                               <  2:147210/150‑1 (MQ=255)
                                                                                          ccAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAAcg                                                             >  2:869879/1‑150 (MQ=255)
                                                                                              cATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGc                                                         <  2:1510103/150‑1 (MQ=255)
                                                                                               aTCTCTTCGTTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGAGCACGTCGCTGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGcc                                                        <  2:1146156/150‑1 (MQ=255)
                                                                                                tctcTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCt                                                       >  1:128281/1‑150 (MQ=255)
                                                                                                 ctctTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAAt                                                                  >  1:312822/1‑138 (MQ=255)
                                                                                                 ctctTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTg                                                      >  1:52285/1‑150 (MQ=255)
                                                                                                  tctTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGc                                                     >  1:945914/1‑150 (MQ=255)
                                                                                                  tctTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGATCGGCAATAAACGCGGCCTGc                                                     >  1:1007464/1‑150 (MQ=255)
                                                                                                     tCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCAcc                                                  <  2:389246/150‑1 (MQ=255)
                                                                                                         tCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGAt                                              <  1:456262/150‑1 (MQ=255)
                                                                                                            ccGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACTGGATCCg                                           >  2:593661/1‑150 (MQ=255)
                                                                                                            ccGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCg                                           >  2:71062/1‑150 (MQ=255)
                                                                                                            ccGCCATCTCCAGATTCATGCGGGTACCCAGCGGCTGACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCg                                           <  1:1587310/150‑1 (MQ=255)
                                                                                                             cGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCgg                                          <  2:1427263/150‑1 (MQ=255)
                                                                                                              gCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCggg                                         >  2:344090/1‑150 (MQ=255)
                                                                                                              gCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCggg                                         <  1:346430/150‑1 (MQ=255)
                                                                                                                  tctcCAGATTCATGCGGGTATCCTGCGTCTGACGCAGGATGCGCACGGCGCGGTCAGTAATGTGACGTCGATCTTCACGTAAATGCACGTTAATGCCGTCCGCTCCCGCCTGCTCTGCAATAAACGCGGCCTGCACCGGATCCGGGTAAg                                     <  2:318324/150‑1 (MQ=255)
                                                                                                                  tctcCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGAACTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAg                                     >  2:281784/1‑150 (MQ=255)
                                                                                                                    tcgtgATGCATGCGGGTATCGAGCGTCTGACGCAGGATGCGCACGTCGCGGGCAGTAATGTGACGGCTTTCTTCACGTAAATGCACGGTAATGCCGTCCGCTCGGGCCTGCTCGGCAATTTACGCGGCCTGCACCGTATCCGGGTAAGCg                                   <  1:1339014/146‑1 (MQ=255)
                                                                                                                    tcCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCg                                   >  2:527716/1‑150 (MQ=255)
                                                                                                                     ccAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTTCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCgg                                  >  2:1009311/1‑150 (MQ=255)
                                                                                                                      cAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGt                                 >  1:756530/1‑150 (MQ=255)
                                                                                                                       aGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTa                                <  2:1498371/150‑1 (MQ=255)
                                                                                                                            cATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcg                           <  1:363053/150‑1 (MQ=255)
                                                                                                                             aTGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgc                          <  2:128281/150‑1 (MQ=255)
                                                                                                                              tGCGGGTATCCAGCGTCTGCCGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAACCGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcg                         >  1:111104/1‑150 (MQ=255)
                                                                                                                              tGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcg                         >  2:1414617/1‑150 (MQ=255)
                                                                                                                              tGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcg                         >  1:477073/1‑150 (MQ=255)
                                                                                                                              tGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACcgcgcg                         <  2:52285/150‑1 (MQ=255)
                                                                                                                                  ggTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCTATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGtt                     <  2:1141322/150‑1 (MQ=255)
                                                                                                                                    caTCCTGCGTCAGACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTgc                   >  2:1575569/2‑150 (MQ=255)
                                                                                                                                    caTCCTGCGTCAGACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTgc                   >  2:1315235/2‑150 (MQ=255)
                                                                                                                                    caTCCTGCGTCAGACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCAGGGTAAGCGGTACCGCGCGCGTTgc                   >  1:1035047/2‑150 (MQ=255)
                                                                                                                                       ccAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCa                <  1:869879/150‑1 (MQ=255)
                                                                                                                                          gCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCg             <  1:71062/150‑1 (MQ=255)
                                                                                                                                          gCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCg             <  1:746777/150‑1 (MQ=255)
                                                                                                                                             tcagACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCGTAg          <  1:1575569/147‑1 (MQ=255)
                                                                                                                                             gcagACGCTGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCGTAg          <  1:1315235/147‑1 (MQ=255)
                                                                                                                                                gACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCGTAGCGa       >  1:824686/1‑150 (MQ=255)
                                                                                                                                                gACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCGTAGCGa       <  2:711420/150‑1 (MQ=255)
                                                                                                                                                 aCGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGAt                                              >  1:488037/1‑110 (MQ=255)
                                                                                                                                                 aCGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGAt                                              <  2:488037/110‑1 (MQ=255)
                                                                                                                                                   gCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCGTAGCGatat    >  2:499557/1‑150 (MQ=255)
                                                                                                                                                    cAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCGTAGCGATATg   <  1:491509/150‑1 (MQ=255)
                                                                                                                                                     aGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCGTAGCGATATgg  <  1:976421/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGCGACATCCAGGCCGCCTTCGGTTGTTACTTCCTGACGCTTTTCCGGTACCAGGCAGCAAAAATGTGGCTTCGTCTCAACGGCGATCGCCAGCATCTCTTCGGTCACCGCCATCTCCAGATTCATGCGGGTATCCAGCGTCTGACGCAGGATGCGCACGTCGCGGTCAGTAATGTGACGGCGATCTTCACGTAAATGCACGGTAATGCCGTCCGCTCCCGCCTGCTCGGCAATAAACGCGGCCTGCACCGGATCCGGGTAAGCGGTACCGCGCGCGTTGCGCAGCGTAGCGATATGG  >  NC_000913/2701399‑2701697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: