Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,712,130 T→A 9.8% Q212L (CAG→CTG)  trmN ← tRNA1(Val) (adenine(37)‑N6)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,712,1300TA 9.8% 282.1 / 14.8 109Q212L (CAG→CTG) trmNtRNA1(Val) (adenine(37)‑N6)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (52/44);  new base A (5/6);  total (57/52)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01

Only 100 of 109 total aligned reads displayed.

ACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAA  >  NC_000913/2711982‑2712273
                                                                                                                                                    |                                                                                                                                               
aCTGCTCACCAATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTg                                                                                                                                                <  2:1213258/150‑1 (MQ=255)
    cTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCggg                                                                                                                                            >  1:342395/1‑150 (MQ=255)
    cTCACCAATTACGGTCCGTCGATCCTTTACCCCGGCAATCATTACATAAACAGATAAAAATCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGAGCACGAAAGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCggg                                                                                                                                            >  1:152511/1‑150 (MQ=255)
        cccATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGgaga                                                                                                                                        >  1:64741/1‑150 (MQ=255)
         ccATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGACCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCAGGCGGGAGAAt                                                                                                                                      <  2:673562/150‑1 (MQ=255)
           aTTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAATGTTTGTTAAGCGGCGTATACGGTTATGAATAGTTCTTTTCTGGCTAACGAATGACTAAGCGATAGCTATAGCATTCTCCCCGCAGGCGGGAGAATg                                                                                                                                     <  2:196657/150‑1 (MQ=255)
            ttCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGc                                                                                                                                    >  1:624274/1‑150 (MQ=255)
            ttCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCCGCAGGCGGGAGAATGc                                                                                                                                    <  1:1692691/150‑1 (MQ=255)
            gtcaggTCAGGCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAAGAGTTCTGGGCTGGCCCACGAATGACTAAGCGAGCGCTAAAGCATTCTCCCCGCAGGCGGGAGAATGc                                                                                                                                    <  1:281808/146‑1 (MQ=255)
             tccggtccggCGATCCTATCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGTAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGcc                                                                                                                                   <  2:1090185/150‑1 (MQ=255)
             tCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCCGCAGGCGGGAGAAt                                                                                                                                      <  1:1134375/147‑1 (MQ=255)
             tCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCCGCAGGCGGGAGAAt                                                                                                                                      >  2:1134375/1‑147 (MQ=255)
             tCCGGTCCGTCGATCCTTTCCCCCGGAAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGcc                                                                                                                                   <  2:1504893/150‑1 (MQ=255)
              ccGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCca                                                                                                                                  <  1:191362/150‑1 (MQ=255)
               cGGTCCGCCGAGCCTTGCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGAGCTGGTCTGGCCCACGAATGACTAAGCGAGCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCcag                                                                                                                                 <  1:1282784/150‑1 (MQ=255)
                 gTCCGTCGATCCTTTCCCCCGGTAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCCGCAGGCGGGAGAATGctttag                                                                                                                               <  2:445091/150‑6 (MQ=255)
                 gTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCCGCAGGCGGGAGAATGctttag                                                                                                                               <  2:638634/150‑6 (MQ=255)
                   ccGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCagcagc                                                                                                                             >  1:947505/1‑150 (MQ=255)
                     gTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCAc                                                                                                                           <  2:873839/150‑1 (MQ=255)
                     gTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCAc                                                                                                                           >  1:573746/1‑150 (MQ=255)
                           ccTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATg                                                                                                                     <  1:783106/150‑1 (MQ=255)
                            cTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGc                                                                                                                    >  2:468227/1‑150 (MQ=255)
                               tCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGc                                                                                                                 <  2:1518727/150‑1 (MQ=255)
                                cccccGGCAATCATTTCATAAACAGATAAAAAGCCTGGGTCAGCGCCGAATACGCTTCGGAATTGTTCTGGTCTGGCCCACGAATGAATAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCa                                                                                                                >  2:1370713/1‑150 (MQ=255)
                                cccccGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGGGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCCCGAATGCATAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCa                                                                                                                >  2:1364068/1‑150 (MQ=255)
                                cccccGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCAGGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCa                                                                                                                >  1:323788/1‑150 (MQ=255)
                                cccccGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCa                                                                                                                >  2:1658127/1‑150 (MQ=255)
                                cccccGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTACGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGTAGAATGCCACCAGAACCCGATGCGGCa                                                                                                                >  1:332577/1‑150 (MQ=255)
                                  cccGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAACGCATTCTCCCGCCTGCGGGGAGAATGCCCGCAGCACCCGATGCGGCAGt                                                                                                              >  2:1694893/1‑150 (MQ=255)
                                  cccGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGt                                                                                                              >  2:615601/1‑150 (MQ=255)
                                  cccGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGt                                                                                                              >  1:763091/1‑150 (MQ=255)
                                       cAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCgcgc                                                                                                         >  2:1186065/1‑150 (MQ=255)
                                           cATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCg                                                                                                     >  2:1289987/1‑150 (MQ=255)
                                           cATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCg                                                                                                     >  1:1595027/1‑150 (MQ=255)
                                            aTTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACTCTTCGGAATAGTTCTGGGCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGt                                                                                                    <  2:67452/150‑1 (MQ=255)
                                               aCATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGtttt                                                                                                 >  2:607885/1‑150 (MQ=255)
                                               aCATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGtttt                                                                                                 <  2:780260/150‑1 (MQ=255)
                                                  tAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCTGCAGTCGCGCTTCGTTTTCCg                                                                                              <  2:573746/150‑1 (MQ=255)
                                                  tAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCg                                                                                              <  2:1483128/150‑1 (MQ=255)
                                                  tAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCg                                                                                              <  1:1506304/150‑1 (MQ=255)
                                                   aaaCAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGTAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGc                                                                                             <  2:763091/150‑1 (MQ=255)
                                                   aaaCAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGc                                                                                             <  1:728140/150‑1 (MQ=255)
                                                    aaCAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGcc                                                                                            >  1:1558322/1‑150 (MQ=255)
                                                      cAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCcac                                                                                          <  2:1595027/150‑1 (MQ=255)
                                                      cAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCcac                                                                                          <  1:607885/150‑1 (MQ=255)
                                                      cAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCcac                                                                                          >  1:5936/1‑150 (MQ=255)
                                                      cAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCcac                                                                                          >  2:1078491/1‑150 (MQ=255)
                                                      cAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAAGAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGTGGAGAATGCCAGCAGCACACGATGCGGCAGTCGCGCTTCGTTGTCCGCcac                                                                                          >  2:1078490/1‑150 (MQ=255)
                                                         aTAAAAAGCCTGTGTCAGCGCCGGATACGCTTCGGTAAAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATGCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtc                                                                                       <  2:339835/150‑1 (MQ=255)
                                                         aTAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtc                                                                                       >  1:795878/1‑150 (MQ=255)
                                                          tAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtcc                                                                                      >  1:911479/1‑150 (MQ=255)
                                                             aaaGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACAtccgtc                                                                                   <  2:610040/150‑1 (MQ=255)
                                                                gCCTGGGTCAGCGCCGTATAAGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCGTGCGGGGAGAATGCCCGCAGCACCCGATGCGGCAGTCGCGCTTCGGTTTCCGCCACATCCGTCCGc                                                                                >  2:1296978/1‑150 (MQ=255)
                                                                 ccTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCa                                                                               >  1:472470/1‑150 (MQ=255)
                                                                 ccTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCa                                                                               >  2:1603060/1‑150 (MQ=255)
                                                                  cTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCGTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCaa                                                                              >  1:1039222/1‑150 (MQ=255)
                                                                  cTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCaa                                                                              >  2:1627475/1‑150 (MQ=255)
                                                                           cgcCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATg                                                                     >  1:616742/1‑150 (MQ=255)
                                                                             ccGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGcc                                                                   <  1:1076854/150‑1 (MQ=255)
                                                                             ccGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGcc                                                                   <  2:1181873/150‑1 (MQ=255)
                                                                                    cGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCAt                                                            <  2:64741/150‑1 (MQ=255)
                                                                                         cGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCATACGTAAATGCCAGCCCATGCTTa                                                       >  2:532308/1‑150 (MQ=255)
                                                                                          ggAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTaa                                                      >  2:374705/1‑150 (MQ=255)
                                                                                            aaTAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATg                                                    <  1:683827/150‑1 (MQ=255)
                                                                                               aGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCa                                                 >  2:1544345/1‑150 (MQ=255)
                                                                                                    tggtctggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCCGCCCATGCTTAATGCCAGCTcc                                            >  1:1284759/1‑150 (MQ=255)
                                                                                                    tggtctggCCCACGAATGACTAAGCGATCGCTAAAGCATGCTCCAGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAACTGCCAGCCCCTGCTTAATGCCAGCTcc                                            <  1:1694893/150‑1 (MQ=255)
                                                                                                       tctggCCCACGAATGACTAAGCGATCGCTATAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTa                                         <  2:152511/150‑1 (MQ=255)
                                                                                                         tggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTaaa                                       <  2:715304/150‑1 (MQ=255)
                                                                                                          ggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTaaaa                                      <  1:1186357/150‑1 (MQ=255)
                                                                                                          ggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTaaaa                                      >  1:507007/1‑150 (MQ=255)
                                                                                                          ggCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTaaaa                                      <  1:1627475/150‑1 (MQ=255)
                                                                                                           gCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAAc                                     >  2:625314/1‑150 (MQ=255)
                                                                                                           gCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAAc                                     <  1:1544345/150‑1 (MQ=255)
                                                                                                            cccACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAAcc                                    >  1:838296/1‑150 (MQ=255)
                                                                                                             ccACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCCACCGTACATGCCAGCCCATGCTTAATGCCCGCTCCGTAAACCCa                                   >  1:1303894/1‑150 (MQ=255)
                                                                                                              cACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTa                                         <  2:910565/143‑1 (MQ=255)
                                                                                                              cACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTa                                         >  1:910565/1‑143 (MQ=255)
                                                                                                                  aaTGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTAcc                              >  2:1539216/1‑150 (MQ=255)
                                                                                                                     gACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAAt                           <  2:947505/150‑1 (MQ=255)
                                                                                                                        tAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTg                        <  2:472470/150‑1 (MQ=255)
                                                                                                                        tAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTg                        <  1:1289987/150‑1 (MQ=255)
                                                                                                                            cGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCACACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGctct                    >  1:1435055/1‑150 (MQ=255)
                                                                                                                              aTCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTgg                  <  1:575169/150‑1 (MQ=255)
                                                                                                                               tCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGgc                 <  1:1153034/150‑1 (MQ=255)
                                                                                                                               tCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCAGGCTTAATGCCAGCTCCGTAAAACCATGACCAATGTGctggggc                 >  1:859215/1‑145 (MQ=255)
                                                                                                                                cGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGgca                <  1:1078491/150‑1 (MQ=255)
                                                                                                                                cGCTAAAGCATGCTCCCGCCGGCGGGGAGAATGCCAGCAGCACCCGATGCGGGAGGCGCGCTTCGTTTTCCGCCACAGCCGTCCGCCAACGTAACTGCCAGCCCATGCTTAATGCCAGCTCCGTAACCCCATTACCAATCTGCTCTGgca                <  1:1078490/150‑1 (MQ=255)
                                                                                                                                  cTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGgcagc              <  2:342395/150‑1 (MQ=255)
                                                                                                                                    aaaGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCAc            <  2:332577/150‑1 (MQ=255)
                                                                                                                                       gCATTCTCCCGCCTGCGGGGAGAAAGCCAGCAGCACCCGATGCGGTAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTATGGCAGCACTAc         >  2:1328727/1‑150 (MQ=255)
                                                                                                                                        cATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACg        >  2:511282/1‑150 (MQ=255)
                                                                                                                                        cATTCTCCCCGCAGGCGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACg        >  1:1162683/1‑150 (MQ=255)
                                                                                                                                         aTTCTCCCGCCTGCGGGTAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGc       <  2:624274/150‑1 (MQ=255)
                                                                                                                                         aTTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGc       <  1:1364068/150‑1 (MQ=255)
                                                                                                                                         aTTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGc       <  1:1370713/150‑1 (MQ=255)
                                                                                                                                          ttCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCa      <  2:1589617/150‑1 (MQ=255)
                                                                                                                                          ttCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCAATGCGGACTGCCTGCTCCGTAAACCCATTACCTATCTGCTCTGGCAGCACTaggta      >  2:645603/1‑146 (MQ=255)
                                                                                                                                             tcCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAGCCATTACCAATCTGCTCTGGCAGCACTACGCAGaa   >  1:839831/1‑150 (MQ=255)
                                                                                                                                              cccGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCTGCTCCGTTAAACCATTACCAATCTGCGCTGGCAGCCCTACGCAGaaa  >  2:1045673/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
ACTGCTCACCCATTCCGGTCCGTCGATCCTTTCCCCCGGCAATCATTACATAAACAGATAAAAAGCCTGGGTCAGCGCCGTATACGCTTCGGAATAGTTCTGGTCTGGCCCACGAATGACTAAGCGATCGCTAAAGCATTCTCCCGCCTGCGGGGAGAATGCCAGCAGCACCCGATGCGGCAGTCGCGCTTCGTTTTCCGCCACATCCGTCCGCAAACGTAAATGCCAGCCCATGCTTAATGCCAGCTCCGTAAAACCATTACCAATCTGCTCTGGCAGCACTACGCAGAAA  >  NC_000913/2711982‑2712273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: