Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,718,554 A→T 5.6% intergenic (‑26/‑181) yfiF ← / → trxC putative methyltransferase/thioredoxin 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,718,5540AT 5.6% 341.2 / 5.5 117intergenic (‑26/‑181)yfiF/trxCputative methyltransferase/thioredoxin 2
Reads supporting (aligned to +/- strand):  ref base A (55/55);  new base T (5/2);  total (60/57)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.67e-01

Only 100 of 119 total aligned reads displayed.

TTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGCGTCGTTGCCG  >  NC_000913/2718406‑2718703
                                                                                                                                                    |                                                                                                                                                     
ttttCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAg                                                                                                                                                      <  1:1315926/150‑1 (MQ=255)
ttttCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTGGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGaat                                                                                                                                                      <  1:1315928/150‑2 (MQ=255)
      tGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCa                                                                                                                                                >  2:862481/1‑150 (MQ=255)
        gACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGa                                                                                                                                              <  1:905464/150‑1 (MQ=255)
            cccgcccCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCCCTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTGTTCTCCACCAGCGCTGCGCGCAGCGCGCAGCTTAc                                                                                                                                          >  1:1138294/1‑150 (MQ=255)
            cccgcccCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATAATCATCACTGCGGACATACATCACTTTGAACTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCCCCAGCGCTGAGCGAAGCGCGCAGATTAc                                                                                                                                          >  2:1547737/1‑150 (MQ=255)
             ccgcccCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGCTTCGCGCAgcgctgg                                                                                                                                         <  1:931687/150‑7 (MQ=255)
              cgcccCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTAccc                                                                                                                                        >  1:1505324/1‑150 (MQ=255)
              cgcccCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTCCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTAccc                                                                                                                                        <  2:1530672/150‑1 (MQ=255)
              cgcacCCTTTCCCGGTACTCGGGTTGAGGGTACGTTTATCAGAATCATAATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGGTCATGCTTTTCTCCACCACCGCTGCGCGAAGCGCGCAGATTAccc                                                                                                                                        <  1:1095633/150‑1 (MQ=255)
               gcccCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCAGCACTGCGGACATACTTCACTTTGCCCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCg                                                                                                                                       >  2:1138444/1‑150 (MQ=255)
                  cccTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAg                                                                                                                                    <  1:391535/150‑1 (MQ=255)
                  cccTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTATCCACCACCGCTGCGCGAAGCGCGCATATTACCCGAAg                                                                                                                                    >  2:356734/1‑150 (MQ=255)
                         ccGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCcgcgc                                                                                                                             >  2:1702643/1‑150 (MQ=255)
                         ccGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGCTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCcgcgc                                                                                                                             >  2:764598/1‑150 (MQ=255)
                          cGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCcgcgcg                                                                                                                            <  1:404173/150‑1 (MQ=255)
                           ggTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACGTTGCCGCTTTTACCTTTCATTTCATCGTTCATGGTTTTCTCCACCAGCGCTGAGCGAAGCGCGCAGATTTCCCGAAGTCCGCGCgg                                                                                                                           <  2:796554/150‑1 (MQ=255)
                             tACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGtt                                                                                                                         >  2:888275/1‑150 (MQ=255)
                               cgcgGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAATCGCGCAGATTACCCGAAGTCCGCGAGGTTCg                                                                                                                       >  2:159648/1‑150 (MQ=255)
                               cgcgGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCg                                                                                                                       >  2:1350302/1‑150 (MQ=255)
                                 cgGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGcc                                                                                                                     <  1:782470/150‑1 (MQ=255)
                                  gggTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCa                                                                                                                    >  1:1512549/1‑150 (MQ=255)
                                    gTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAACCGCGCGGACTTCGGGTAATCTGCGCGCTTCGcgcag                                                                                                                  <  1:1061481/150‑5 (MQ=37)
                                         gggTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTc                                                                                                             >  2:1214389/1‑150 (MQ=255)
                                         gggTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTc                                                                                                             >  1:143525/1‑150 (MQ=255)
                                          ggTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATTCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCg                                                                                                            >  1:651326/1‑150 (MQ=255)
                                             aCGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAc                                                                                                         <  1:1499637/150‑1 (MQ=255)
                                             aCGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAc                                                                                                         <  1:808025/150‑1 (MQ=255)
                                              cGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAcc                                                                                                        >  2:183240/1‑150 (MQ=255)
                                              cGTTTATCAGAATCATCATCACAGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTACTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTAcc                                                                                                        <  2:328317/150‑1 (MQ=255)
                                                   aTCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGc                                                                                                   <  2:950049/150‑1 (MQ=255)
                                                   aTCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGc                                                                                                   <  1:1472139/150‑1 (MQ=255)
                                                    tCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGcc                                                                                                  <  1:605952/150‑1 (MQ=255)
                                                    tCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGCTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGcc                                                                                                  <  1:764598/150‑1 (MQ=255)
                                                       gcatcatcatcACTCCGTACATACCTCACTTAGCCCTTGCCGCTTTTACCTTTCCATTCATCGTTCATGCGTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGc                                                                                               <  2:309605/148‑1 (MQ=255)
                                                        aaTCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCg                                                                                              <  1:508973/150‑1 (MQ=255)
                                                         atcatcatcACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGa                                                                                             <  1:997797/150‑1 (MQ=255)
                                                           catcatcaCTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCg                                                                                              >  1:1622336/1‑147 (MQ=255)
                                                             tcatcaCTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTAt                                                                                         <  1:1350302/150‑1 (MQ=255)
                                                              catcaCTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATc                                                                                        <  2:519253/150‑1 (MQ=255)
                                                               atcaCTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa                                                                                       >  1:1123648/1‑150 (MQ=255)
                                                               atcaCTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCa                                                                                       <  1:1679294/150‑1 (MQ=255)
                                                                 caCTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATa                                                                                     <  1:144979/150‑1 (MQ=255)
                                                                 caCTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATa                                                                                     >  2:738262/1‑150 (MQ=255)
                                                                   ctgctgACTTTCCTCCCTGTGTCCTGGCCGCGTTTACCTTTCATTGCATCGTGCATGCGTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCAGCGCGGTGCGCCATGATTTCCTACCAAAGCCTGCGACTATCATAcc                                                                                   <  1:258289/150‑1 (MQ=255)
                                                                   atGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATAcc                                                                                   <  2:1638243/149‑1 (MQ=255)
                                                                      cGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTAt                                                                                >  1:1092783/1‑150 (MQ=255)
                                                                        gACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTa                                                                                                          >  2:1335075/1‑122 (MQ=255)
                                                                        gACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTg                                                                              >  2:1341063/1‑150 (MQ=255)
                                                                        gACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCAGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTg                                                                              >  1:734496/1‑150 (MQ=255)
                                                                          catacatCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGaa                                                                            >  2:1491644/1‑150 (MQ=255)
                                                                            tacatCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCTCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATa                                                                          >  1:97059/1‑150 (MQ=255)
                                                                            tacatCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGTGACTATCATACCTATTGAATa                                                                          >  2:949399/1‑150 (MQ=255)
                                                                            tacatCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATa                                                                          <  1:1341063/150‑1 (MQ=255)
                                                                             acatCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATaa                                                                         <  2:1482978/150‑1 (MQ=255)
                                                                                tCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCAGCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAAc                                                                      <  2:1473515/150‑1 (MQ=255)
                                                                                 cACTTTGACCTTGCCGCTTTTACGTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGTAGTCCGCGCTGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACa                                                                     <  1:159648/150‑1 (MQ=255)
                                                                                 cACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACa                                                                     >  1:942780/1‑150 (MQ=255)
                                                                                 cACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACa                                                                     <  2:423509/150‑1 (MQ=255)
                                                                                   cTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGa                                                                   >  2:530874/1‑150 (MQ=255)
                                                                                         ccTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGAttgttg                                                             >  2:940559/1‑150 (MQ=255)
                                                                                         ccTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGAttgttg                                                             <  1:1066594/150‑1 (MQ=255)
                                                                                         ccTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGAGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGAttgttg                                                             >  2:896625/1‑150 (MQ=255)
                                                                                             gCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTg                                                         <  1:888275/150‑1 (MQ=255)
                                                                                              ccGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTgg                                                        <  2:905045/150‑1 (MQ=255)
                                                                                                 cTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAAc                                                     >  2:832879/1‑150 (MQ=255)
                                                                                                   tttACCTTTCATTTCATCGTTCATTCTGTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACaa                                                   <  1:1547737/150‑1 (MQ=255)
                                                                                                     tACCTTTCATTTCATCGTTCATGCTTTTCTCTACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATg                                                 >  2:739800/1‑150 (MQ=255)
                                                                                                       ccTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTc                                               >  2:276274/1‑150 (MQ=255)
                                                                                                        cTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTcc                                              >  1:387638/1‑150 (MQ=255)
                                                                                                        atttcctttcaTCGTTCTTGCTTTTCTCCACAAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCTCGCGGTTCGCCATGATTTCGTACCAATGCCTGCGTCTATCATACCTATTGACTAAAACAGATTGTTGTCTTGAACAATGTcc                                              <  2:146653/149‑1 (MQ=255)
                                                                                                          ttcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTcccc                                            <  2:1505324/150‑1 (MQ=255)
                                                                                                          ttcatttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTcccc                                            <  1:1491644/150‑1 (MQ=255)
                                                                                                              tttcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGata                                        >  1:1711807/1‑150 (MQ=255)
                                                                                                                tcatCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGataat                                      <  1:132198/150‑1 (MQ=255)
                                                                                                                 catCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTCCCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGataata                                     <  2:1085084/150‑1 (MQ=255)
                                                                                                                     gTTCATGCTTTTCTCCACCAGCGATGCGCCAAGCGCGCAGATTACCCGAAGTCCGCGCGCTTCGCCAAGATTTCGTACCAAAGCCTGCGACTATCAAACCTATTGAATAAAACAGATTGTTGTCTCGACCAATGTCCCCGATAATATCTa                                 >  2:1444496/1‑150 (MQ=255)
                                                                                                                       tCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAAc                               <  2:587569/150‑1 (MQ=255)
                                                                                                                          tGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGat                                         <  2:1518557/137‑1 (MQ=255)
                                                                                                                            cTTTTCTCCACGAGCGCTGCTCGAAGCGCGCAGATTAACCGAAGTCCTCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATAtt                          <  1:219197/150‑1 (MQ=255)
                                                                                                                            cTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATAtt                          >  1:1344680/1‑150 (MQ=255)
                                                                                                                              tttCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAg                        >  1:1242606/1‑150 (MQ=255)
                                                                                                                              tttCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAg                        >  1:884515/1‑150 (MQ=255)
                                                                                                                                tctcCACCAGCGCTGCGGGATGCGCGCAGATTACCCGAAGTCCGCGCGTTTCGCAATTTTTTCGTACCAAAGCCTGCTACTATCATACCTATTTAATAAAACAGATTGTTGTCTGCAACAATGTCCCCTATAATATGTAAAATATTATaa                      <  2:387638/150‑1 (MQ=255)
                                                                                                                                tctcCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTTGaa                      >  2:1528406/1‑150 (MQ=255)
                                                                                                                                 ctcCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGaaa                     <  2:346898/150‑1 (MQ=255)
                                                                                                                                     accaGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATa                 <  2:884515/150‑1 (MQ=255)
                                                                                                                                      ccaGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATAc                >  2:1648923/1‑150 (MQ=255)
                                                                                                                                      ccaGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATAc                >  2:1396662/1‑150 (MQ=255)
                                                                                                                                        aGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCg              >  1:1507725/1‑150 (MQ=255)
                                                                                                                                         gcgcTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCgg             <  2:1092783/150‑1 (MQ=255)
                                                                                                                                         aatcTGCGCGCTTCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATTAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCgg             >  2:962636/4‑150 (MQ=255)
                                                                                                                                         aatcTGCGCGCTTCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCgg             >  1:693622/4‑150 (MQ=255)
                                                                                                                                         aatcTGCGCGCTTCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCgg             >  2:138262/4‑150 (MQ=255)
                                                                                                                                          atcTGCGCGCTTCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGc            >  2:184797/3‑150 (MQ=255)
                                                                                                                                           gcTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGcg           >  1:1213365/1‑150 (MQ=255)
                                                                                                                                             tGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGcgtc         <  2:1242606/150‑1 (MQ=255)
                                                                                                                                              gcgcGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGcgtcg        >  2:406741/1‑150 (MQ=255)
                                                                                                                                                 cgAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCGATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATTAAACAGATTGTTGTCTGGCACAATGTACCCGATAATATGTAACATATTAGAAACATACCGGcgtcgtag     >  1:48172/1‑148 (MQ=255)
                                                                                                                                                    aGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGCGTCGTTGCCg  <  2:942780/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCGCTGCGCGAAGCGCGCAGATTACCCGAAGTCCGCGCGGTTCGCCATGATTTCGTACCAAAGCCTGCGACTATCATACCTATTGAATAAAACAGATTGTTGTCTGGAACAATGTCCCCGATAATATGTAACATATTAGAAACATACCGGCGTCGTTGCCG  >  NC_000913/2718406‑2718703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: