Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,724,095 C→A 9.7% intergenic (+13/‑33) pssA → / → yfiM phosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,724,0950CA 9.7% 313.8 / 19.2 120intergenic (+13/‑33)pssA/yfiMphosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (50/58);  new base A (5/7);  total (55/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

Only 100 of 124 total aligned reads displayed.

TGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATGCTTTCC  >  NC_000913/2723946‑2724244
                                                                                                                                                     |                                                                                                                                                       
tGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATACGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTc                                                                                                                                                         >  2:671465/1‑150 (MQ=255)
 gATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTcc                                                                                                                                                        >  1:1568932/1‑150 (MQ=255)
 gATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTcc                                                                                                                                                        >  2:1214837/1‑150 (MQ=255)
         aGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACggg                                                                                                                                                <  1:558239/150‑1 (MQ=255)
           cATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgt                                                                                                                                              <  2:1174218/150‑1 (MQ=255)
             tACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttg                                                                                                                                            <  1:201382/150‑2 (MQ=255)
             tACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttg                                                                                                                                            <  2:424247/150‑2 (MQ=255)
               ccaccaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtg                                                                                                                                          <  1:897091/150‑4 (MQ=255)
               accccaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtg                                                                                                                                          <  1:1466069/147‑4 (MQ=255)
                 accaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGGAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttg‑‑ttt                                                                                                                                      >  1:824458/1‑150 (MQ=255)
                  ccaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtgatt                                                                                                                                       <  2:887840/150‑7 (MQ=255)
                  ccaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtgatt                                                                                                                                       <  1:1596763/150‑7 (MQ=255)
                    aTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑tttttt                                                                                                                                   >  2:924387/1‑150 (MQ=255)
                    aTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑tttttt                                                                                                                                   >  2:123916/1‑150 (MQ=255)
                     tggTTAAGCAATATCGCGATCTGTATAGTATTGCCGATTATCCGTTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCGGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTg                                                                                                                                  <  1:496771/148‑1 (MQ=255)
                     tCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTg                                                                                                                                  <  2:553727/150‑1 (MQ=255)
                      cGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTAACCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTCTCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑tttttgga                                                                                                                                 <  1:1286656/150‑4 (MQ=255)
                        ttAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAg                                                                                                                               <  1:641349/150‑1 (MQ=255)
                         tAAGCACTATCTCCATCTGCAAAGTAAGGCCGATTATCCGGTGACGGTTCGTAAACTCATCCGCCGTTTGCGCCGTAGCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAgg                                                                                                                              <  1:147728/150‑1 (MQ=255)
                         tAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAgg                                                                                                                              >  2:1516363/1‑150 (MQ=255)
                           aGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGcc                                                                                                                            <  2:1507647/150‑1 (MQ=255)
                            ccACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCa                                                                                                                           <  1:1081962/149‑1 (MQ=255)
                              aCTATCGCGATCTGCAAAGTATTTCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCTCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACg                                                                                                                         <  2:549071/150‑1 (MQ=255)
                              aCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACg                                                                                                                         >  1:1558054/1‑150 (MQ=255)
                              aCTATCGCGAGCTGCAAAGTATTGCCGATTATCCGGGGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACg                                                                                                                         <  1:1501327/150‑1 (MQ=255)
                                   cgcgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTg                                                                                                                    <  2:1513383/150‑1 (MQ=255)
                                    gcgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGc                                                                                                                   <  1:1558947/150‑1 (MQ=255)
                                    gcgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCATGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGc                                                                                                                   <  2:1054710/150‑1 (MQ=255)
                                     cgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCg                                                                                                                  >  1:1121387/1‑150 (MQ=255)
                                      gATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGt                                                                                                                 <  2:697089/150‑1 (MQ=255)
                                         cTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTAtt                                                                                                              <  1:1501972/150‑1 (MQ=255)
                                           gCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCt                                                                                                            >  2:1544247/1‑150 (MQ=255)
                                           gCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCt                                                                                                            >  1:974665/1‑150 (MQ=255)
                                             aaaGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCttt                                                                                                          <  2:1568932/150‑1 (MQ=255)
                                              aaGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCtttt                                                                                                         >  2:756665/1‑150 (MQ=255)
                                              aaGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCtttt                                                                                                         <  1:1152629/150‑1 (MQ=255)
                                              aaGTATTGCCGATTATCCGGTGAAGGTGCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGAATTCtttt                                                                                                         <  1:1336827/150‑1 (MQ=255)
                                                gTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTg                                                                                                       <  2:1657258/150‑1 (MQ=255)
                                                 tATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGt                                                                                                      >  2:1258534/1‑150 (MQ=255)
                                                  aTTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTc                                                                                                     <  2:481549/150‑1 (MQ=255)
                                                     gCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGt                                                                                                  >  1:1032486/1‑150 (MQ=255)
                                                     gCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGt                                                                                                  <  2:1347288/150‑1 (MQ=255)
                                                     gCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATAAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGt                                                                                                  >  1:1309204/1‑150 (MQ=255)
                                                        gATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCg                                                                                               <  1:698371/150‑1 (MQ=255)
                                                           tATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTa                                                                                            >  1:426991/1‑150 (MQ=255)
                                                            aTCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCTTCGACCGATTTATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTAt                                                                                           <  1:652475/150‑1 (MQ=255)
                                                              ccGGTGAAGGTTCGTAAACTCCTCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGGTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTg                                                                                         >  2:244545/1‑150 (MQ=255)
                                                              ccGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTAt                                                                                           <  2:1679050/150‑1 (MQ=255)
                                                               cGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGtt                                                                                       >  2:460623/1‑150 (MQ=255)
                                                                    aaGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACtt                                                                                   <  1:1226240/150‑1 (MQ=255)
                                                                       gTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCt                                                                                >  2:15554/1‑150 (MQ=255)
                                                                           gTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGAt                                                                            <  2:601867/150‑1 (MQ=255)
                                                                            tAAACTCATCCGCCGTTTGCGCCGTATCCGCATCTACCGATTAATTAGCCGCATCCTGTAATCACAACCAAGTCATGTACGGGGTTTG‑‑TTTTTTGGAGGCCACCTTTTGCGTATTCTTTTTGTCTGTTGGCTATTGTTACTTTCTGGATg                                                                           >  1:1125419/1‑150 (MQ=255)
                                                                            tAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATg                                                                           <  1:1516363/150‑1 (MQ=255)
                                                                             aaaCTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGc                                                                          >  2:169174/1‑150 (MQ=255)
                                                                              aaCTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCa                                                                         <  1:169174/150‑1 (MQ=255)
                                                                                  cATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTTTTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCa                                                                     >  1:281566/1‑150 (MQ=255)
                                                                                  cATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCa                                                                     <  1:752624/150‑1 (MQ=255)
                                                                                  cATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCa                                                                     >  1:1016603/1‑150 (MQ=255)
                                                                                   aTCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCat                                                                    <  1:843/150‑1 (MQ=255)
                                                                                   aTCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCat                                                                    <  1:201094/150‑1 (MQ=255)
                                                                                      cgccgTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATg                                                                 >  2:914059/1‑150 (MQ=255)
                                                                                           tttGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTaa                                                            <  1:924387/150‑1 (MQ=255)
                                                                                            ttGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTCCGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAAc                                                           <  2:260779/150‑1 (MQ=255)
                                                                                              gcgcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCt                                                              >  2:1489739/1‑145 (MQ=255)
                                                                                              gcgcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCt                                                              <  1:1489739/145‑1 (MQ=255)
                                                                                                gcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCCCGTTTTGCGTATTCTTTTTTTCTGTTCGCTATTGTTAAATTCTGGATGCAGCCATATGGCTAACGATa                                                       >  2:612694/1‑150 (MQ=255)
                                                                                                 ccGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAg                                                      <  1:804171/150‑1 (MQ=255)
                                                                                                 ccGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGCCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAg                                                      >  2:1013175/1‑150 (MQ=255)
                                                                                                  cGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGc                                                     <  1:756665/150‑1 (MQ=255)
                                                                                                      tCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTggc                                                 >  2:1446902/1‑149 (MQ=255)
                                                                                                       ccGGATCGACCGATTAATTCGCCGCATCCTGTAATCACAACCCCGTCCTGAACGGGGTTTG‑‑TTTTTTGGAGGCCACGCTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAg                                                <  2:169053/150‑1 (MQ=255)
                                                                                                         gCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCg                                              >  2:748464/1‑150 (MQ=255)
                                                                                                            tCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGc                                           <  1:123916/150‑1 (MQ=255)
                                                                                                              gACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAg                                         >  2:1435205/1‑150 (MQ=255)
                                                                                                                ccGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGa                                       <  2:1379136/150‑1 (MQ=255)
                                                                                                                ccGATCAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGGTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTATTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGTGGGCAGGa                                       >  1:38689/1‑150 (MQ=255)
                                                                                                                  gATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTTGAGCGGGCAGGATa                                     >  1:1642547/1‑150 (MQ=255)
                                                                                                                      aattaGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGc                                 >  2:250145/1‑150 (MQ=255)
                                                                                                                      aattaGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTACTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGc                                 <  2:824458/150‑1 (MQ=255)
                                                                                                                       attaGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCt                                >  1:1465340/1‑150 (MQ=255)
                                                                                                                         taGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCa                              >  2:1377865/1‑150 (MQ=255)
                                                                                                                          aGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTATTTGGAGGCCACGTTTTGCGTATTCATTTTGTCTGATCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCaa                             <  1:292510/150‑1 (MQ=255)
                                                                                                                           gCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTATCGATAGCTGGAGCGGGCAGGATAAAGCTCAac                            >  1:1162570/1‑150 (MQ=255)
                                                                                                                           gCCGCATCCTGTAATCACAACCCCGTCCGGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAac                            <  1:260082/150‑1 (MQ=255)
                                                                                                                              gCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACt                         <  1:35497/150‑1 (MQ=255)
                                                                                                                                  ccTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTAt                     >  2:1087081/1‑150 (MQ=255)
                                                                                                                                   cTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACAATTTATc                    >  1:1649531/1‑150 (MQ=255)
                                                                                                                                   cTGTAATCACAACCCCGTCCTGTAAGGGGTTTG‑‑TTTTTTGGAGGCCAGGTTTTGCGTTTTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGTCTAACGATAGCGGGAGCGGGCAGGATAAAGCTGAACTCTTTAta                    <  2:1133022/150‑2 (MQ=255)
                                                                                                                                        aaacaaaCCCCGTACAGGACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTc               >  2:1653050/6‑150 (MQ=255)
                                                                                                                                         tCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCg              <  2:1183484/150‑1 (MQ=255)
                                                                                                                                          cacaACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCgg             <  2:821688/150‑1 (MQ=255)
                                                                                                                                           acaACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGc            >  2:909591/1‑150 (MQ=255)
                                                                                                                                            caACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGTCAGGATAAAGCTCACCACTTTATCGCCTCGGCg           >  1:274900/1‑150 (MQ=255)
                                                                                                                                             aaCCCCGTACAGGACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGa          >  1:732801/1‑150 (MQ=255)
                                                                                                                                             aaCCCCGTACAGGACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGa          >  1:1459574/1‑150 (MQ=255)
                                                                                                                                             aaCCCCGTACAGGACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTCTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGa          >  1:990847/1‑150 (MQ=255)
                                                                                                                                                cccGTCCTGTCCGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGCATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATGc       <  2:1558054/150‑1 (MQ=255)
                                                                                                                                                    tCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCATCACTTTATCGCCTCGGCGATGCTTTc   >  2:1313731/1‑150 (MQ=255)
                                                                                                                                                     ccTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCGAGGCGAtaaagtgt  <  1:1544247/150‑8 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
TGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATGCTTTCC  >  NC_000913/2723946‑2724244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: