Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,724,097 T→A 9.8% intergenic (+15/‑31) pssA → / → yfiM phosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,724,0970TA 9.8% 313.4 / 19.6 121intergenic (+15/‑31)pssA/yfiMphosphatidylserine synthase; CDP‑diacylglycerol‑serine O‑phosphatidyltransferase/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (50/58);  new base A (5/7);  total (55/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.86e-01

Only 100 of 124 total aligned reads displayed.

AGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATGCTTTCCGC  >  NC_000913/2723955‑2724246
                                                                                                                                              |                                                                                                                                                       
aGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACggg                                                                                                                                                  <  1:558239/150‑1 (MQ=255)
  cATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCTTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCACATCCTGTAATCACAACCCCGTCCTGTACGGGgt                                                                                                                                                <  2:158331/150‑1 (MQ=255)
  cATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgt                                                                                                                                                <  2:1174218/150‑1 (MQ=255)
    tACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttg                                                                                                                                              <  1:201382/150‑2 (MQ=255)
      ccaccaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtg                                                                                                                                            <  1:897091/150‑4 (MQ=255)
      ccaccaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCAGTCCTGTAGGGGgtttg‑‑t                                                                                                                                          >  2:1523183/1‑150 (MQ=255)
      accccaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtg                                                                                                                                            <  1:1466069/147‑4 (MQ=255)
        accaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGGAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttg‑‑ttt                                                                                                                                        >  1:824458/1‑150 (MQ=255)
         ccaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtgatt                                                                                                                                         <  2:887840/150‑7 (MQ=255)
         ccaTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTACAGGACGGGgttgtgatt                                                                                                                                         <  1:1596763/150‑7 (MQ=255)
           aTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑tttttt                                                                                                                                     >  2:924387/1‑150 (MQ=255)
           aTCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑tttttt                                                                                                                                     >  2:123916/1‑150 (MQ=255)
            tggTTAAGCAATATCGCGATCTGTATAGTATTGCCGATTATCCGTTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCGGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTg                                                                                                                                    <  1:496771/148‑1 (MQ=255)
             cGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTAACCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTCTCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑tttttgga                                                                                                                                   <  1:1286656/150‑4 (MQ=255)
              gTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGa                                                                                                                                  >  1:236872/1‑150 (MQ=255)
               ttAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAg                                                                                                                                 <  1:641349/150‑1 (MQ=255)
                tAAGCACTATCTCCATCTGCAAAGTAAGGCCGATTATCCGGTGACGGTTCGTAAACTCATCCGCCGTTTGCGCCGTAGCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAgg                                                                                                                                <  1:147728/150‑1 (MQ=255)
                  aGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGcc                                                                                                                              <  2:1507647/150‑1 (MQ=255)
                   ccACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCa                                                                                                                             <  1:1081962/149‑1 (MQ=255)
                     aCTATCGCGATCTGCAAAGTATTTCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCTCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACg                                                                                                                           <  2:549071/150‑1 (MQ=255)
                     aCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACg                                                                                                                           >  1:1558054/1‑150 (MQ=255)
                     aCTATCGCGAGCTGCAAAGTATTGCCGATTATCCGGGGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACg                                                                                                                           <  1:1501327/150‑1 (MQ=255)
                        aTCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGttt                                                                                                                        >  1:557531/1‑150 (MQ=255)
                          cgcgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTg                                                                                                                      <  2:1513383/150‑1 (MQ=255)
                           gcgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGc                                                                                                                     <  1:1558947/150‑1 (MQ=255)
                           gcgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCATGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGc                                                                                                                     <  2:1054710/150‑1 (MQ=255)
                            cgATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCg                                                                                                                    >  1:1121387/1‑150 (MQ=255)
                             gATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGt                                                                                                                   <  2:697089/150‑1 (MQ=255)
                                cTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTAtt                                                                                                                <  1:1501972/150‑1 (MQ=255)
                                 tGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTc                                                                                                               >  1:821688/1‑150 (MQ=255)
                                  gCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCt                                                                                                              >  2:1544247/1‑150 (MQ=255)
                                  gCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCt                                                                                                              >  1:974665/1‑150 (MQ=255)
                                     aaGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCtttt                                                                                                           <  1:1152629/150‑1 (MQ=255)
                                     aaGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCtttt                                                                                                           >  2:756665/1‑150 (MQ=255)
                                     aaGTATTGCCGATTATCCGGTGAAGGTGCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGAATTCtttt                                                                                                           <  1:1336827/150‑1 (MQ=255)
                                       gTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTg                                                                                                         <  2:1657258/150‑1 (MQ=255)
                                        tATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGt                                                                                                        >  2:1258534/1‑150 (MQ=255)
                                         aTTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTc                                                                                                       <  2:481549/150‑1 (MQ=255)
                                           tGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTg                                                                                                     <  2:455754/150‑1 (MQ=255)
                                            gCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGt                                                                                                    >  1:1032486/1‑150 (MQ=255)
                                            gCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATAAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGt                                                                                                    >  1:1309204/1‑150 (MQ=255)
                                                  tATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTa                                                                                              >  1:426991/1‑150 (MQ=255)
                                                   aTCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCTTCGACCGATTTATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTAt                                                                                             <  1:652475/150‑1 (MQ=255)
                                                     ccGGTGAAGGTTCGTAAACTCCTCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGGTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTg                                                                                           >  2:244545/1‑150 (MQ=255)
                                                     ccGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTGTTTTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTAt                                                                                             <  2:1679050/150‑1 (MQ=255)
                                                      cGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGtt                                                                                         >  2:460623/1‑150 (MQ=255)
                                                           aaGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACtt                                                                                     <  1:1226240/150‑1 (MQ=255)
                                                              gTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCt                                                                                  >  2:15554/1‑150 (MQ=255)
                                                                tCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTTATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTgg                                                                                <  1:700972/150‑1 (MQ=255)
                                                                  gTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGAt                                                                              <  2:601867/150‑1 (MQ=255)
                                                                   tAAACTCATCCGCCGTTTGCGCCGTATCCGCATCTACCGATTAATTAGCCGCATCCTGTAATCACAACCAAGTCATGTACGGGGTTTG‑‑TTTTTTGGAGGCCACCTTTTGCGTATTCTTTTTGTCTGTTGGCTATTGTTACTTTCTGGATg                                                                             >  1:1125419/1‑150 (MQ=255)
                                                                   tAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATg                                                                             <  1:1516363/150‑1 (MQ=255)
                                                                     aaCTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCa                                                                           <  1:169174/150‑1 (MQ=255)
                                                                         cATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTTTTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCa                                                                       >  1:281566/1‑150 (MQ=255)
                                                                         cATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCa                                                                       >  1:1016603/1‑150 (MQ=255)
                                                                         cATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCa                                                                       <  1:752624/150‑1 (MQ=255)
                                                                          aTCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCat                                                                      <  1:201094/150‑1 (MQ=255)
                                                                          aTCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCat                                                                      <  1:843/150‑1 (MQ=255)
                                                                             cgccgTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATg                                                                   >  2:914059/1‑150 (MQ=255)
                                                                                   ttGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTCCGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAAc                                                             <  2:260779/150‑1 (MQ=255)
                                                                                     gcgcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCt                                                                <  1:1489739/145‑1 (MQ=255)
                                                                                     gcgcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCt                                                                >  2:1489739/1‑145 (MQ=255)
                                                                                       gcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCCCGTTTTGCGTATTCTTTTTTTCTGTTCGCTATTGTTAAATTCTGGATGCAGCCATATGGCTAACGATa                                                         >  2:612694/1‑150 (MQ=255)
                                                                                       gcCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCtgcatcca                                                         <  2:1474592/150‑8 (MQ=255)
                                                                                        ccGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGCCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAg                                                        >  2:1013175/1‑150 (MQ=255)
                                                                                         cGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGc                                                       <  1:756665/150‑1 (MQ=255)
                                                                                              ccGGATCGACCGATTAATTCGCCGCATCCTGTAATCACAACCCCGTCCTGAACGGGGTTTG‑‑TTTTTTGGAGGCCACGCTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAg                                                  <  2:169053/150‑1 (MQ=255)
                                                                                               cGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGc                                                 >  2:201727/1‑150 (MQ=255)
                                                                                                gCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCg                                                >  2:748464/1‑150 (MQ=255)
                                                                                                   tCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGc                                             <  1:123916/150‑1 (MQ=255)
                                                                                                     gACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAg                                           >  2:1435205/1‑150 (MQ=255)
                                                                                                       ccGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGa                                         <  2:1379136/150‑1 (MQ=255)
                                                                                                       ccGATCAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGGTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTATTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGTGGGCAGGa                                         >  1:38689/1‑150 (MQ=255)
                                                                                                         gATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATa                                       >  2:1110023/1‑150 (MQ=255)
                                                                                                             aattaGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGc                                   >  2:250145/1‑150 (MQ=255)
                                                                                                             aattaGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTACTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGc                                   <  2:824458/150‑1 (MQ=255)
                                                                                                              attaGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCt                                  >  1:1465340/1‑150 (MQ=255)
                                                                                                                taGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCa                                >  2:1377865/1‑150 (MQ=255)
                                                                                                                 aGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTATTTGGAGGCCACGTTTTGCGTATTCATTTTGTCTGATCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCaa                               <  1:292510/150‑1 (MQ=255)
                                                                                                                  gCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTATCGATAGCTGGAGCGGGCAGGATAAAGCTCAac                              >  1:1162570/1‑150 (MQ=255)
                                                                                                                  gCCGCATCCTGTAATCACAACCCCGTCCGGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAac                              <  1:260082/150‑1 (MQ=255)
                                                                                                                     gCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACt                           <  1:35497/150‑1 (MQ=255)
                                                                                                                          cTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACAATTTATc                      >  1:1649531/1‑150 (MQ=255)
                                                                                                                          cTGTAATCACAACCCCGTCCTGTAAGGGGTTTG‑‑TTTTTTGGAGGCCAGGTTTTGCGTTTTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGTCTAACGATAGCGGGAGCGGGCAGGATAAAGCTGAACTCTTTAta                      <  2:1133022/150‑2 (MQ=255)
                                                                                                                              aaTCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCt                  <  2:557531/150‑1 (MQ=255)
                                                                                                                               aaacaaaCCCCGTACAGGACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTc                 >  2:1653050/6‑150 (MQ=255)
                                                                                                                                tCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCg                <  2:1183484/150‑1 (MQ=255)
                                                                                                                                aacaaaCCCCGTACAGGACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCg                >  1:1602692/5‑150 (MQ=255)
                                                                                                                                 cacaACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCgg               <  2:821688/150‑1 (MQ=255)
                                                                                                                                  acaACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGc              >  2:909591/1‑150 (MQ=255)
                                                                                                                                   caACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGTCAGGATAAAGCTCACCACTTTATCGCCTCGGCg             >  1:274900/1‑150 (MQ=255)
                                                                                                                                    aaCCCCGTACAGGACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGa            >  1:1459574/1‑150 (MQ=255)
                                                                                                                                    aaCCCCGTACAGGACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGa            >  1:732801/1‑150 (MQ=255)
                                                                                                                                    aaCCCCGTACAGGACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTCTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGa            >  1:990847/1‑150 (MQ=255)
                                                                                                                                     aCCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGAt           >  2:1359003/1‑150 (MQ=255)
                                                                                                                                       cccGTCCTGTCCGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGCATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATGc         <  2:1558054/150‑1 (MQ=255)
                                                                                                                                         cGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATGCtt       >  1:960868/1‑150 (MQ=255)
                                                                                                                                           tCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCATCACTTTATCGCCTCGGCGATGCTTTc     >  2:1313731/1‑150 (MQ=255)
                                                                                                                                            ccTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCGAGGCGAtaaagtgt    <  1:1544247/150‑8 (MQ=255)
                                                                                                                                              tGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATGCTTTccgc  >  2:134077/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                       
AGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG‑‑TTTTTTGGAGGCCACGTTTTGCGTATTCTTTTTGTCTGTTCGCTATTGTTACTTTCTGGATGCAGCCATATGGCTAACGATAGCTGGAGCGGGCAGGATAAAGCTCAACACTTTATCGCCTCGGCGATGCTTTCCGC  >  NC_000913/2723955‑2724246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: