Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,736,913 T→A 8.0% intergenic (+30/‑241) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,736,9130TA 8.0% 300.9 / 13.5 113intergenic (+30/‑241)bamD/raiABamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
Reads supporting (aligned to +/- strand):  ref base T (53/51);  new base A (5/4);  total (58/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-02

Only 100 of 114 total aligned reads displayed.

CCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAA  >  NC_000913/2736769‑2737062
                                                                                                                                                |                                                                                                                                                     
ccAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGc                                                                                                                                                  <  1:1071323/150‑1 (MQ=255)
ccAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGc                                                                                                                                                  <  2:1572712/150‑1 (MQ=255)
 cAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGAAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCt                                                                                                                                                 >  2:1506231/1‑150 (MQ=255)
   ggCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGc                                                                                                                                               >  2:510399/1‑150 (MQ=255)
    gCTACGCGTGATGTGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGcc                                                                                                                                              <  1:917031/150‑1 (MQ=255)
    gCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGcc                                                                                                                                              <  1:165114/150‑1 (MQ=255)
    gCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGcc                                                                                                                                              >  1:232854/1‑150 (MQ=255)
       aCGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGtt                                                                                                                                           <  1:1472615/150‑1 (MQ=255)
         gcgTGATGCGCTGCCGCTGATGTAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGtttt                                                                                                                                         <  2:1498111/150‑1 (MQ=255)
         gcgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttt                                                                                                                                         <  2:312448/150‑1 (MQ=255)
          cgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttttt                                                                                                                                        >  1:632144/1‑150 (MQ=255)
          cgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttttt                                                                                                                                        >  2:759784/1‑150 (MQ=255)
           gTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttttt                                                                                                                                       <  2:955747/150‑1 (MQ=255)
           gTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttttt                                                                                                                                       <  2:180352/150‑1 (MQ=255)
           gTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttttt                                                                                                                                       <  2:203964/150‑1 (MQ=255)
            tGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGttttgtg                                                                                                                                      <  2:1456930/150‑4 (MQ=255)
            tGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttttttt                                                                                                                                      >  1:658779/1‑150 (MQ=255)
            tGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttttttt                                                                                                                                      >  2:909551/1‑150 (MQ=255)
            tGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACAGCAGCCCTTGAGCTGCCGttttttt                                                                                                                                      >  1:1705851/1‑150 (MQ=255)
                 cgcTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAATATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCt                                                                                                                                 >  2:947469/1‑150 (MQ=255)
                    tGCCGCTGATGGAAAATGCATACTGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTc                                                                                                                              >  2:1423520/1‑150 (MQ=255)
                    tGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGta                                                                                                                              >  1:412848/1‑149 (MQ=255)
                    tGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCG‑TTTTTTATTCTGTCa                                                                                                                             <  2:632144/150‑1 (MQ=255)
                     gCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCa                                                                                                                             >  2:131765/1‑150 (MQ=255)
                       cGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGt                                                                                                                           <  2:1002556/150‑1 (MQ=255)
                            attgAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGa                                                                                                                      <  1:1528653/147‑1 (MQ=255)
                            aTGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGa                                                                                                                      <  2:658779/150‑1 (MQ=255)
                              ggAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGaaa                                                                                                                    >  2:1236958/1‑150 (MQ=255)
                               gAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGaaaa                                                                                                                   >  2:1648197/1‑149 (MQ=255)
                                aaaaTGCATACCGTCAGATTCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACt                                                                                                                  <  1:1514312/150‑1 (MQ=255)
                                   aTGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACAGAACCGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTGTTTTATTCTGGCAAGAGTGAAACAGaa                                                                                                               >  1:878564/1‑150 (MQ=255)
                                    tGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAg                                                                                                              >  2:1182459/1‑150 (MQ=255)
                                    tGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAg                                                                                                              <  1:964816/150‑1 (MQ=255)
                                       aTACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGa                                                                                                           >  1:1608603/1‑150 (MQ=255)
                                       aTACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTCAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGa                                                                                                           <  1:260319/150‑1 (MQ=255)
                                        tACCGTCAGATGCAGATGAATGAGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGGTTTTTTATTATGTCAGTTGTGAAACTGAAGCGaa                                                                                                          >  1:1425438/1‑149 (MQ=255)
                                               atatgaagatgAATGCGCAAGCTGAAAATGTAGCTAAAATCATCGCCGCAAACAGCAGCAATAAATAACAGAAACCTTAAACACAAAACGGCAGCCCTTGTGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCg                                                                                                   <  2:1447905/148‑1 (MQ=255)
                                               agatgcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCg                                                                                                   <  2:892189/150‑1 (MQ=255)
                                                        tgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAtc                                                                                          <  2:1283441/150‑1 (MQ=255)
                                                           aTGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtca                                                                                       <  2:56429/150‑1 (MQ=255)
                                                             gcgcAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtcatc                                                                                     <  1:1182459/150‑1 (MQ=255)
                                                              cgcAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCATCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtcatca                                                                                    <  2:875792/150‑1 (MQ=255)
                                                              cgcAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtcatca                                                                                    >  2:1557195/1‑150 (MQ=255)
                                                              cgaaaGCTGAAAAAGTAGAGAAAATCATCGCCGCAAACAGCATCAATACATAACTGAAACCTGAAACACAAAACGGCATCCCTTGATCTGCCGTTTTTTTATTCTGTCAGTTGTGATACTGAAGCGATTTAGTCGCTATCGATCtcatca                                                                                    <  1:1079284/147‑1 (MQ=255)
                                                                cAAGCTGAAAAAGTAGCGAAAATCATTGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCaaa                                                                                  >  2:1440582/1‑150 (MQ=255)
                                                                cAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGGTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATaaaa                                                                                  >  1:1100748/1‑150 (MQ=255)
                                                                 aaGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAat                                                                                 <  1:1128106/150‑1 (MQ=255)
                                                                   gCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAatat                                                                               >  2:490895/1‑150 (MQ=255)
                                                                   gCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAatat                                                                               <  1:1603460/150‑1 (MQ=255)
                                                                    cTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATg                                                                              >  1:556062/1‑150 (MQ=255)
                                                                     tGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATgg                                                                             >  2:590814/1‑150 (MQ=255)
                                                                       aaaaaGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCt                                                                           <  1:510399/150‑1 (MQ=255)
                                                                              aGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTg                                                                    >  2:373415/1‑150 (MQ=255)
                                                                              aGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTCAAGAGATTTAGTCGCTATAGATCTCATCAAATATGGCACGCATTg                                                                    >  1:736087/1‑150 (MQ=255)
                                                                              aGCGAAAATCAACCCCGCACACCGCAGCAATACCTAACAGCAACCCGAAACACCACACGGCACCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAATTAGCCGCTATCGATCTCATCAAATATGGCTCGCTTTg                                                                    <  2:1122573/150‑1 (MQ=255)
                                                                                 gAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtca                                                                                       <  2:49293/128‑1 (MQ=255)
                                                                                 gAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTgaga                                                                 <  1:1128547/150‑1 (MQ=255)
                                                                                          cgccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCa                                                        <  1:947469/150‑1 (MQ=255)
                                                                                          cgccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACAGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCa                                                        <  2:1705851/150‑1 (MQ=255)
                                                                                           gccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCaa                                                       >  2:523332/1‑150 (MQ=255)
                                                                                           gccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCaa                                                       >  1:412647/1‑150 (MQ=255)
                                                                                            ccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAg                                                      <  1:590814/150‑1 (MQ=255)
                                                                                            ccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAg                                                      <  2:628650/150‑1 (MQ=255)
                                                                                            ccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAg                                                      >  1:548393/1‑150 (MQ=255)
                                                                                             cgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGt                                                     <  1:529160/150‑1 (MQ=255)
                                                                                             cgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGt                                                     >  1:1484090/1‑150 (MQ=255)
                                                                                                  aCAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGCGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCATCAAGTaaaaa                                                >  1:1207125/1‑150 (MQ=255)
                                                                                                     gcagcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGGTGTGAAACTGAATCGATTTAGTCGCTATCGATCTCATCAAATATGGCGCGCTTTGAGATATTCCTCAATTAAAAAAAc                                             >  1:1276553/1‑150 (MQ=255)
                                                                                                       agcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAt                                           >  1:174114/1‑150 (MQ=255)
                                                                                                        gcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAtc                                          >  1:937386/1‑150 (MQ=255)
                                                                                                        gcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCAATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAtc                                          <  2:1120665/150‑1 (MQ=255)
                                                                                                        gcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCATGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAtc                                          >  1:1484926/1‑150 (MQ=255)
                                                                                                          aaTACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAtctc                                        >  2:1503474/1‑150 (MQ=255)
                                                                                                           aTACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATctct                                       <  1:523332/150‑1 (MQ=255)
                                                                                                            tACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCtt                                      <  2:970697/150‑1 (MQ=255)
                                                                                                               aTAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTAATTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCTATTTGAGATATTACTCAAGTATAAAAACATCTCTTcca                                   >  1:322690/1‑149 (MQ=255)
                                                                                                                tAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTgag                                                                  <  2:992395/118‑1 (MQ=255)
                                                                                                                tAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTgag                                                                  >  1:992395/1‑118 (MQ=255)
                                                                                                                tAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTg                                  >  2:728420/1‑150 (MQ=255)
                                                                                                                 aaCAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTTTTCTGTCAGTTGTGAAACTGTAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGc                                 <  2:1036949/150‑1 (MQ=255)
                                                                                                                   cAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTTTTCTGTCAGTTGTTTAACTGAAGCGATTTAGTCGCTATAGATCTCATCAAATTTGGGTAGCTTTTAGATTTTACTAAAGTAAAAAAACAAAACTTCCTGCGa                               >  2:1416574/1‑150 (MQ=255)
                                                                                                                        aCCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTtct                          >  2:493766/1‑150 (MQ=255)
                                                                                                                        aCCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTAATCAAATATGGCTCGCTTTGAGATATTACTCAAGTAAAAAAACATCACTTACTGCGAtttat                          >  2:1572987/1‑148 (MQ=255)
                                                                                                                        aCCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATATCATAAAATATGGCTCGATTTGAGATATTACTCAAGTAAAAAAAAATCTCTTCCTGCGATTtcg                          >  1:1292639/1‑149 (MQ=255)
                                                                                                                          cTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTca                        <  1:1643161/150‑1 (MQ=255)
                                                                                                                            gAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTcaca                      >  1:108812/1‑150 (MQ=255)
                                                                                                                                 acaAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaaaaa                 <  1:1236958/150‑1 (MQ=255)
                                                                                                                                  aaaaaCGGCAGCTCAAGGGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTACGATTTCTCACaaaaaaa                >  2:288347/2‑149 (MQ=255)
                                                                                                                                  aaaaaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAg                <  1:373415/149‑1 (MQ=255)
                                                                                                                                    aaaCGGCAGCTCAAGGGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAt              >  1:1669689/1‑150 (MQ=255)
                                                                                                                                     aaCGGCAGCTCAAGGGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGAtt             >  1:1385837/1‑150 (MQ=255)
                                                                                                                                      aCGGCAGCTCAAGGGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTAACAAAAAAGATTc            >  2:145839/1‑150 (MQ=255)
                                                                                                                                          cAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAATTATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTg        <  2:1606197/150‑1 (MQ=255)
                                                                                                                                          cAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTg        <  1:1423520/150‑1 (MQ=255)
                                                                                                                                          cAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTg        <  1:490895/150‑1 (MQ=255)
                                                                                                                                          cAGCACTTTAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTg        <  2:528580/150‑1 (MQ=255)
                                                                                                                                            gCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGAc      >  1:1361550/1‑150 (MQ=255)
                                                                                                                                             cccTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACa     >  1:261002/1‑150 (MQ=255)
                                                                                                                                               cTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACaaa   >  2:934566/1‑150 (MQ=255)
                                                                                                                                                ttGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACaaaa  >  1:1651603/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
CCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGACAAAA  >  NC_000913/2736769‑2737062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑