Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,738,925 G→T 10.3% intergenic (+20/+23) pheA → / ← tyrA chorismate mutase and prephenate dehydratase, P‑protein/fused chorismate mutase T/prephenate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,738,9250GT10.3% 270.7 / 18.7 97intergenic (+20/+23)pheA/tyrAchorismate mutase and prephenate dehydratase, P‑protein/fused chorismate mutase T/prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (43/43);  new base T (5/5);  total (49/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-01

Only 100 of 105 total aligned reads displayed.

GGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGC  >  NC_000913/2738776‑2739074
                                                                                                                                                     |                                                                                                                                                     
ggATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGatgatg                                                                                                                                                       <  1:359457/150‑1 (MQ=255)
ggATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGatgatg                                                                                                                                                       >  2:280499/1‑150 (MQ=255)
 tatatTCAGGCCAATCTTGAATCAGCTGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGAtgt                                                                                                                                                      <  1:1670687/149‑1 (MQ=255)
  atatTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGAtgtg                                                                                                                                                     <  1:511525/150‑1 (MQ=255)
  atatTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGAtgtg                                                                                                                                                     <  1:1459430/150‑1 (MQ=255)
     ttCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAat                                                                                                                                                  >  2:1114326/1‑150 (MQ=255)
       cAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAatca                                                                                                                                                >  1:179730/1‑150 (MQ=255)
          gCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATcc                                                                                                                                             >  2:1238822/1‑150 (MQ=255)
           ccAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCg                                                                                                                                            >  2:449880/1‑150 (MQ=255)
            cAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATTCACATCATCCgg                                                                                                                                           <  2:1345830/150‑1 (MQ=255)
            cAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCgg                                                                                                                                           <  2:814249/150‑1 (MQ=255)
            cAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCgg                                                                                                                                           <  1:550905/150‑1 (MQ=255)
             aaTCTTGAATCAGCGGAAATTCAAAAATCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATTCACATCATCCGGc                                                                                                                                          <  1:669782/150‑1 (MQ=255)
             aaTCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATTCACATCATCCGGc                                                                                                                                          <  1:819293/150‑1 (MQ=255)
             aaTCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATTCACATCATCCGGc                                                                                                                                          <  2:40997/150‑1 (MQ=255)
              aTCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATTCACATCATCCGGCa                                                                                                                                         <  1:956091/150‑1 (MQ=255)
                 ttGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTg                                                                                                                                      <  2:1678668/150‑1 (MQ=255)
                  tGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTgg                                                                                                                                     >  1:1286206/1‑150 (MQ=255)
                  tGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTAACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTgg                                                                                                                                     >  1:1283362/1‑150 (MQ=255)
                       cAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGCTCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGattat                                                                                                                                >  2:1075942/1‑150 (MQ=255)
                       cAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGattat                                                                                                                                >  1:469768/1‑150 (MQ=255)
                         gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGAttatta                                                                                                                              <  1:2976/150‑1 (MQ=255)
                           tgAAATGCAAAAAGAATTGAAAGAGTTAGGTTAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATTATGTGAATCATCCGGCACTGGATTATTACt                                                                                                                            <  1:1719951/149‑1 (MQ=255)
                           ggAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTCATGAAAAGGTGCCGGATGATGTGAATCATCCGGAACTGGATTATTACt                                                                                                                            >  1:1346816/1‑150 (MQ=255)
                                tGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGa                                                                                                                       >  2:1041861/1‑150 (MQ=255)
                                  cAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGAtt                                                                                                                     <  1:229437/150‑1 (MQ=255)
                                  cAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGAtt                                                                                                                     >  1:979514/1‑150 (MQ=255)
                                   aaaaaGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTg                                                                                                                    >  2:507116/1‑150 (MQ=255)
                                    aaaaGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGt                                                                                                                   <  1:449880/150‑1 (MQ=255)
                                        gCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCAtt                                                                                                               >  2:1307119/1‑150 (MQ=255)
                                         cATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTc                                                                                                              >  2:676098/1‑150 (MQ=255)
                                            tGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGATCGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGcc                                                                                                           >  2:379112/1‑150 (MQ=255)
                                             gAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCt                                                                                                          >  2:156926/1‑150 (MQ=255)
                                               aaGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACAGGCGAATGACAATCGccagt                                                                                                        <  1:1496976/150‑4 (MQ=255)
                                                 tagTTAGGGGAAATCACGCGTTCAATGAAGGTTTTGGGCTGTTACCCAAGTGAGAACGTTGTGCCTTTTGATCCAACCTGATTAAATGGTGCCGTATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACg                                                                                                      <  2:576967/149‑1 (MQ=255)
                                                 gattatggggAAAGCACACGTTCAATGACTGTTTTGGGCGGTTACCGAAGTGAGAACGTTGTGCCTGTTGTTCCAACCTGACGAATTGGTGCGGGTTGATGTGATTCATCCGGAACTGGATTATTACTGGCGATTGTCATTCGCCTGACg                                                                                                      <  2:71677/144‑1 (MQ=255)
                                                 gagTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGAATGACAATCGccagtaa                                                                                                      <  1:1468667/150‑6 (MQ=255)
                                                     tatcggAAATCACCCGTTCAATGAAGGAATTGGGCTGTGACCGAAGTGAGACCGTAGTGCCTGTTGATCCAACCTGATGAAAAGGGGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAAt                                                                                                  <  1:863835/146‑1 (MQ=255)
                                                     tAGGGGAAATCACCCGTTCAATTAAGGTAATGTGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTTATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAAt                                                                                                  <  1:869745/150‑1 (MQ=255)
                                                         ggAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAaca                                                                                              >  1:426319/1‑150 (MQ=255)
                                                         ggAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACATGATGAAAGGGTGCCGGATGATGTGAATCATCCGTCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAacc                                                                                              >  2:1554098/1‑149 (MQ=255)
                                                          gAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAacac                                                                                             >  2:4232/1‑150 (MQ=255)
                                                           aaaTCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACAcg                                                                                            >  1:1622292/1‑150 (MQ=255)
                                                            aaTCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACAcgc                                                                                           <  1:1242881/150‑1 (MQ=255)
                                                               cACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGc                                                                                        >  2:1001657/1‑150 (MQ=255)
                                                               aaCCCGTTCAATGTAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGc                                                                                        <  2:874734/149‑1 (MQ=255)
                                                                aCCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTAGATCCAACCTGATGAAAAGGTGCCGGATGATCTCAATCATCCGGCACTGTATTATAACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCt                                                                                       >  2:1449404/1‑150 (MQ=255)
                                                                 cccGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCtt                                                                                      <  1:676098/150‑1 (MQ=255)
                                                                 cccGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCtt                                                                                      <  1:229404/150‑1 (MQ=255)
                                                                  ccGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCttt                                                                                     <  2:426319/150‑1 (MQ=255)
                                                                   cGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTc                                                                                    <  1:543876/150‑1 (MQ=255)
                                                                   cGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTc                                                                                    <  2:1451180/150‑1 (MQ=255)
                                                                    gTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGAAGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCa                                                                                   >  2:1554198/1‑150 (MQ=255)
                                                                     ttCAATTAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAAACTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCAc                                                                                  <  2:1231717/150‑1 (MQ=255)
                                                                      tCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACGGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCAct                                                                                 <  2:159347/150‑1 (MQ=255)
                                                                         aTGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTg                                                                              <  2:744335/150‑1 (MQ=255)
                                                                         aTGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTg                                                                              >  1:28255/1‑150 (MQ=255)
                                                                           gAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGaa                                                                            >  1:1316518/1‑150 (MQ=255)
                                                                            aaGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGaaa                                                                           <  2:624266/150‑1 (MQ=255)
                                                                                  tggggCGGTTACCCAGTTGTGAACGTAGGGCCTGTTGATCCAACCTGTTGAAATGGGGCCGGAGGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAACCGCtg                                                                     <  1:849077/148‑1 (MQ=255)
                                                                                    gggCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCtgtg                                                                   <  1:280499/150‑1 (MQ=255)
                                                                                    gggCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCtgtg                                                                   <  2:447091/150‑1 (MQ=255)
                                                                                     ggCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGc                                                                  <  1:4232/150‑1 (MQ=255)
                                                                                            aCCCATGTGAGAACGTTGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGTATGATGTTAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCg                                                           <  2:1526112/150‑1 (MQ=255)
                                                                                             cccAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAACGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGc                                                          <  2:1221906/150‑1 (MQ=255)
                                                                                              ccAAGTGAGAACGTAGTGCCTGTTGATCCAACCTTATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGcc                                                         <  1:1130773/150‑1 (MQ=255)
                                                                                               cAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCg                                                        >  2:21958/1‑150 (MQ=255)
                                                                                               cAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCg                                                        >  1:171513/1‑150 (MQ=255)
                                                                                               cAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCAATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCg                                                        >  1:1346733/1‑150 (MQ=255)
                                                                                                 aGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGaa                                                      <  1:112814/150‑1 (MQ=255)
                                                                                                   tGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCCGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAAcc                                                    >  2:662919/1‑150 (MQ=255)
                                                                                                     agaACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGCCGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAg                                                  >  1:1290185/1‑150 (MQ=255)
                                                                                                     agaACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAg                                                  >  1:1283528/1‑150 (MQ=255)
                                                                                                        aCGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGc                                               <  2:527235/150‑1 (MQ=255)
                                                                                                            aGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCa                                           >  2:1466511/1‑150 (MQ=255)
                                                                                                                       aTCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGaaa                                >  2:1082603/1‑150 (MQ=255)
                                                                                                                       aTCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGaaa                                >  1:1361534/1‑150 (MQ=255)
                                                                                                                          cAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTg                             <  1:1114326/150‑1 (MQ=255)
                                                                                                                           aaCCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGCCGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGt                            >  2:667513/1‑150 (MQ=255)
                                                                                                                             ccTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCa                          <  1:737335/150‑1 (MQ=255)
                                                                                                                             ccTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCa                          <  1:1596548/150‑1 (MQ=255)
                                                                                                                             ccTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCa                          >  2:1098213/1‑150 (MQ=255)
                                                                                                                              cTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCaa                         <  1:298895/150‑1 (MQ=255)
                                                                                                                              atgatgaAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATAGCCGAACAAGTGCTCCACCTTGCGGAAACTGTCaa                         >  2:173155/2‑150 (MQ=255)
                                                                                                                               tgatgaAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAAt                        <  2:171513/150‑1 (MQ=255)
                                                                                                                                    aaaaGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACg                   >  1:536871/1‑150 (MQ=255)
                                                                                                                                    aaaaGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACg                   <  2:469768/150‑1 (MQ=255)
                                                                                                                                     aaaGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGc                  >  2:530873/1‑150 (MQ=255)
                                                                                                                                        ggTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTg               >  1:422469/1‑150 (MQ=255)
                                                                                                                                        agTGCCGGATGATTCACATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTg               >  1:1670566/2‑150 (MQ=255)
                                                                                                                                          tGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCt             >  2:466402/1‑150 (MQ=255)
                                                                                                                                           gCCGGATGATTCACATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCtt            >  1:402280/1‑150 (MQ=255)
                                                                                                                                            ccGGATGATTCACATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCCCCTTGCGGAAACTGTCAATAAACGCCTGCTTa           >  2:1038396/1‑150 (MQ=255)
                                                                                                                                            ccGGATGATTCACATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTa           >  2:1569470/1‑150 (MQ=255)
                                                                                                                                            ccGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTACACTCTGAAAACGCTGTGCGTAATCGCCGACCCAGTGCTCCACCTTGCGGAACCTGTCAATAAACGCCTGCTTa           >  2:319377/1‑150 (MQ=255)
                                                                                                                                             cGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTAt          >  1:187951/1‑150 (MQ=255)
                                                                                                                                              ggATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATc         <  2:1078938/150‑1 (MQ=255)
                                                                                                                                                 tgatgTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGATCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGcc      >  1:249992/1‑150 (MQ=255)
                                                                                                                                                  gatgTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGccc     <  1:1349745/150‑1 (MQ=255)
                                                                                                                                                     gtgAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGc  <  1:388563/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGATATTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGC  >  NC_000913/2738776‑2739074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: