Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,738,928 A→C 9.9% intergenic (+23/+20) pheA → / ← tyrA chorismate mutase and prephenate dehydratase, P‑protein/fused chorismate mutase T/prephenate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,738,9280AC 9.9% 282.3 / 19.9 101intergenic (+23/+20)pheA/tyrAchorismate mutase and prephenate dehydratase, P‑protein/fused chorismate mutase T/prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (45/46);  new base C (5/5);  total (50/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 101 total aligned reads displayed.

TTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTC  >  NC_000913/2738781‑2739076
                                                                                                                                                   |                                                                                                                                                    
ttCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAat                                                                                                                                                    >  2:1114326/1‑150 (MQ=255)
  cAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAatca                                                                                                                                                  >  1:179730/1‑150 (MQ=255)
     gCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATcc                                                                                                                                               >  2:1238822/1‑150 (MQ=255)
      ccAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCg                                                                                                                                              >  2:449880/1‑150 (MQ=255)
       cAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATTCACATCATCCgg                                                                                                                                             <  2:1345830/150‑1 (MQ=255)
       cAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCgg                                                                                                                                             <  2:814249/150‑1 (MQ=255)
       cAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCgg                                                                                                                                             <  1:550905/150‑1 (MQ=255)
        aaTCTTGAATCAGCGGAAATTCAAAAATCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATTCACATCATCCGGc                                                                                                                                            <  1:669782/150‑1 (MQ=255)
        aaTCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATTCACATCATCCGGc                                                                                                                                            <  1:819293/150‑1 (MQ=255)
        aaTCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATTCACATCATCCGGc                                                                                                                                            <  2:40997/150‑1 (MQ=255)
         aTCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATTCACATCATCCGGCa                                                                                                                                           <  1:956091/150‑1 (MQ=255)
            ttGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTg                                                                                                                                        <  2:1678668/150‑1 (MQ=255)
             tGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTgg                                                                                                                                       >  1:1286206/1‑150 (MQ=255)
             tGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTAACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTgg                                                                                                                                       >  1:1283362/1‑150 (MQ=255)
                  cAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGCTCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGattat                                                                                                                                  >  2:1075942/1‑150 (MQ=255)
                  cAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGattat                                                                                                                                  >  1:469768/1‑150 (MQ=255)
                    gCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGAttatta                                                                                                                                <  1:2976/150‑1 (MQ=255)
                      tgAAATGCAAAAAGAATTGAAAGAGTTAGGTTAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATTATGTGAATCATCCGGCACTGGATTATTACt                                                                                                                              <  1:1719951/149‑1 (MQ=255)
                      ggAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGCAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGGGAATCATCCGGCACTGGATTATTACt                                                                                                                              <  1:1713440/150‑1 (MQ=255)
                      ggAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTCATGAAAAGGTGCCGGATGATGTGAATCATCCGGAACTGGATTATTACt                                                                                                                              >  1:1346816/1‑150 (MQ=255)
                           tGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGa                                                                                                                         >  2:1041861/1‑150 (MQ=255)
                             cAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGAtt                                                                                                                       >  1:979514/1‑150 (MQ=255)
                             cAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGAtt                                                                                                                       <  1:229437/150‑1 (MQ=255)
                              aaaaaGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTg                                                                                                                      >  2:507116/1‑150 (MQ=255)
                               aaaaGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGt                                                                                                                     <  1:449880/150‑1 (MQ=255)
                                   gCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCAtt                                                                                                                 >  2:1307119/1‑150 (MQ=255)
                                    cATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTc                                                                                                                >  2:676098/1‑150 (MQ=255)
                                       tGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGATCGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGcc                                                                                                             >  2:379112/1‑150 (MQ=255)
                                        gAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCt                                                                                                            >  2:156926/1‑150 (MQ=255)
                                          aaGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACAGGCGAATGACAATCGccagt                                                                                                          <  1:1496976/150‑4 (MQ=255)
                                           agagTTAGGGGAAATCACCCGTGCAATGACGGCATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGAAGGACAATCGccagta                                                                                                         <  1:528558/150‑5 (MQ=255)
                                            tagTTAGGGGAAATCACGCGTTCAATGAAGGTTTTGGGCTGTTACCCAAGTGAGAACGTTGTGCCTTTTGATCCAACCTGATTAAATGGTGCCGTATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACg                                                                                                        <  2:576967/149‑1 (MQ=255)
                                            gattatggggAAAGCACACGTTCAATGACTGTTTTGGGCGGTTACCGAAGTGAGAACGTTGTGCCTGTTGTTCCAACCTGACGAATTGGTGCGGGTTGATGTGATTCATCCGGAACTGGATTATTACTGGCGATTGTCATTCGCCTGACg                                                                                                        <  2:71677/144‑1 (MQ=255)
                                            gagTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGAATGACAATCGccagtaa                                                                                                        <  1:1468667/150‑6 (MQ=255)
                                                tatcggAAATCACCCGTTCAATGAAGGAATTGGGCTGTGACCGAAGTGAGACCGTAGTGCCTGTTGATCCAACCTGATGAAAAGGGGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAAt                                                                                                    <  1:863835/146‑1 (MQ=255)
                                                tAGGGGAAATCACCCGTTCAATTAAGGTAATGTGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTTATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAAt                                                                                                    <  1:869745/150‑1 (MQ=255)
                                                    ggAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAaca                                                                                                >  1:426319/1‑150 (MQ=255)
                                                    ggAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACATGATGAAAGGGTGCCGGATGATGTGAATCATCCGTCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAacc                                                                                                >  2:1554098/1‑149 (MQ=255)
                                                     gAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAacac                                                                                               >  2:4232/1‑150 (MQ=255)
                                                      aaaTCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACAcg                                                                                              >  1:1622292/1‑150 (MQ=255)
                                                       aaTCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACAcgc                                                                                             <  1:1242881/150‑1 (MQ=255)
                                                          cACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGc                                                                                          >  2:1001657/1‑150 (MQ=255)
                                                          aaCCCGTTCAATGTAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGc                                                                                          <  2:874734/149‑1 (MQ=255)
                                                           aCCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTAGATCCAACCTGATGAAAAGGTGCCGGATGATCTCAATCATCCGGCACTGTATTATAACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCt                                                                                         >  2:1449404/1‑150 (MQ=255)
                                                            cccGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCtt                                                                                        <  1:676098/150‑1 (MQ=255)
                                                            cccGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCtt                                                                                        <  1:229404/150‑1 (MQ=255)
                                                             ccGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCttt                                                                                       <  2:426319/150‑1 (MQ=255)
                                                              cGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTc                                                                                      <  1:543876/150‑1 (MQ=255)
                                                              cGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTc                                                                                      <  2:1451180/150‑1 (MQ=255)
                                                               gTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGAAGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCa                                                                                     >  2:1554198/1‑150 (MQ=255)
                                                                ttCAATTAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAAACTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCAc                                                                                    <  2:1231717/150‑1 (MQ=255)
                                                                 tCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACGGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCAct                                                                                   <  2:159347/150‑1 (MQ=255)
                                                                    aTGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTg                                                                                <  2:744335/150‑1 (MQ=255)
                                                                    aTGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTg                                                                                >  1:28255/1‑150 (MQ=255)
                                                                      gAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGaa                                                                              >  1:1316518/1‑150 (MQ=255)
                                                                       aaGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGaaa                                                                             <  2:624266/150‑1 (MQ=255)
                                                                             tggggCGGTTACCCAGTTGTGAACGTAGGGCCTGTTGATCCAACCTGTTGAAATGGGGCCGGAGGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAACCGCtg                                                                       <  1:849077/148‑1 (MQ=255)
                                                                               gggCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCtgtg                                                                     <  2:447091/150‑1 (MQ=255)
                                                                               gggCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCtgtg                                                                     <  1:280499/150‑1 (MQ=255)
                                                                                ggCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGc                                                                    <  1:4232/150‑1 (MQ=255)
                                                                                       aCCCATGTGAGAACGTTGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGTATGATGTTAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCg                                                             <  2:1526112/150‑1 (MQ=255)
                                                                                        cccAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAACGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGc                                                            <  2:1221906/150‑1 (MQ=255)
                                                                                         ccAAGTGAGAACGTAGTGCCTGTTGATCCAACCTTATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGcc                                                           <  1:1130773/150‑1 (MQ=255)
                                                                                          cAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCg                                                          >  1:171513/1‑150 (MQ=255)
                                                                                          cAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCg                                                          >  2:21958/1‑150 (MQ=255)
                                                                                          cAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCAATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCg                                                          >  1:1346733/1‑150 (MQ=255)
                                                                                            aGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGaa                                                        <  1:112814/150‑1 (MQ=255)
                                                                                              tGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCCGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAAcc                                                      >  2:662919/1‑150 (MQ=255)
                                                                                                agaACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGCCGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAg                                                    >  1:1290185/1‑150 (MQ=255)
                                                                                                agaACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAg                                                    >  1:1283528/1‑150 (MQ=255)
                                                                                                   aCGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGc                                                 <  2:527235/150‑1 (MQ=255)
                                                                                                   aCGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGc                                                 <  2:1159781/150‑1 (MQ=255)
                                                                                                       aGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCa                                             >  2:1466511/1‑150 (MQ=255)
                                                                                                                  aTCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGaaa                                  >  2:1082603/1‑150 (MQ=255)
                                                                                                                  aTCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGaaa                                  >  1:1361534/1‑150 (MQ=255)
                                                                                                                     cAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTg                               <  1:1114326/150‑1 (MQ=255)
                                                                                                                      aaCCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGCCGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGt                              >  2:667513/1‑150 (MQ=255)
                                                                                                                        ccTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCa                            <  1:737335/150‑1 (MQ=255)
                                                                                                                        ccTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCa                            <  1:1596548/150‑1 (MQ=255)
                                                                                                                        ccTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCa                            >  2:1098213/1‑150 (MQ=255)
                                                                                                                         cTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCaa                           <  1:298895/150‑1 (MQ=255)
                                                                                                                         atgatgaAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATAGCCGAACAAGTGCTCCACCTTGCGGAAACTGTCaa                           >  2:173155/2‑150 (MQ=255)
                                                                                                                          tgatgaAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAAt                          <  2:171513/150‑1 (MQ=255)
                                                                                                                               aaaaGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACg                     >  1:536871/1‑150 (MQ=255)
                                                                                                                               aaaaGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACg                     <  2:469768/150‑1 (MQ=255)
                                                                                                                                aaaGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGc                    >  2:530873/1‑150 (MQ=255)
                                                                                                                                   ggTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTg                 >  1:422469/1‑150 (MQ=255)
                                                                                                                                   agTGCCGGATGATTCACATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTg                 >  1:1670566/2‑150 (MQ=255)
                                                                                                                                     tGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCt               >  2:466402/1‑150 (MQ=255)
                                                                                                                                      gCCGGATGATTCACATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCtt              >  1:402280/1‑150 (MQ=255)
                                                                                                                                       ccGGATGATTCACATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCCCCTTGCGGAAACTGTCAATAAACGCCTGCTTa             >  2:1038396/1‑150 (MQ=255)
                                                                                                                                       ccGGATGATTCACATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTa             >  2:358911/1‑150 (MQ=255)
                                                                                                                                       ccGGATGATTCACATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTa             >  2:1569470/1‑150 (MQ=255)
                                                                                                                                       ccGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTACACTCTGAAAACGCTGTGCGTAATCGCCGACCCAGTGCTCCACCTTGCGGAACCTGTCAATAAACGCCTGCTTa             >  2:319377/1‑150 (MQ=255)
                                                                                                                                        cGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTAt            >  1:187951/1‑150 (MQ=255)
                                                                                                                                         ggATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATc           <  2:1078938/150‑1 (MQ=255)
                                                                                                                                            tgatgTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGATCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGcc        >  1:249992/1‑150 (MQ=255)
                                                                                                                                             gatgTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGccc       <  1:1349745/150‑1 (MQ=255)
                                                                                                                                                gtgAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGc    <  1:388563/150‑1 (MQ=255)
                                                                                                                                                  gAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTc  <  1:156926/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTCAGGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCAATGAAGGTATTGGGCTGTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGATGAAAAGGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGCGGCTTTCACTCTGAAAACGCTGTGCGTAATCGCCGAACCAGTGCTCCACCTTGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTC  >  NC_000913/2738781‑2739076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: