Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,750,073 G→T 10.6% intergenic (+13/+42) yfjD → / ← grpE UPF0053 family inner membrane protein/heat shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,750,0730GT10.6% 307.1 / 21.3 118intergenic (+13/+42)yfjD/grpEUPF0053 family inner membrane protein/heat shock protein
Reads supporting (aligned to +/- strand):  ref base G (43/62);  new base T (9/4);  total (52/66)
Fisher's exact test for biased strand distribution p-value = 7.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 119 total aligned reads displayed.

TGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGT  >  NC_000913/2749924‑2750218
                                                                                                                                                     |                                                                                                                                                 
tGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGAGATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCg                                                                                                                                                   >  1:1038600/1‑150 (MQ=255)
  gAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGc                                                                                                                                                 <  1:282840/150‑1 (MQ=255)
      agaTCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTc                                                                                                                                             <  1:478250/150‑1 (MQ=255)
      agaTCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTc                                                                                                                                             >  1:1553313/1‑150 (MQ=255)
       gaTCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCg                                                                                                                                            >  2:1602839/1‑150 (MQ=255)
          cccTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCAt                                                                                                                                         <  2:1137059/150‑1 (MQ=255)
          cccTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATAGTCTCGACGTCCAGGACAATATGATGACGCAGGGAAAAGGTTTTCCTGTGAAACCGCTGCGCGAGCGTGTGGCGGAGTAACGAAATCGGCCCGGCATTCGCAt                                                                                                                                         >  1:260214/1‑150 (MQ=255)
                cGCAGGCACCCTCGTGCGTATTGGCGAGTACGATATCGATATTCTCGAGGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAggg                                                                                                                                   <  1:1666175/150‑1 (MQ=255)
                cGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAggg                                                                                                                                   <  1:1672597/150‑1 (MQ=255)
                 gCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGc                                                                                                                                  <  2:529980/150‑1 (MQ=255)
                  cAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATGCGAATGCCGGGcc                                                                                                                                 <  1:1271317/150‑1 (MQ=255)
                  cAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGcc                                                                                                                                 <  1:39314/150‑1 (MQ=255)
                  cAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGcc                                                                                                                                 <  2:651977/150‑1 (MQ=255)
                  cAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGcc                                                                                                                                 <  2:514400/150‑1 (MQ=255)
                  cAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGcc                                                                                                                                 <  1:1110927/150‑1 (MQ=255)
                  cAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGAAATTCTCGACGTACAGGACAATAGGATAAAGCAGGTAAAAGTTATCCCTGAGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATGCGAATGCCGGGcc                                                                                                                                 <  2:1564465/150‑1 (MQ=255)
                   aGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATGCGAATGCCGGGCCg                                                                                                                                <  2:912434/150‑1 (MQ=255)
                    ggCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGt                                                                                                                               >  2:102689/1‑150 (MQ=255)
                       aCCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGaa                                                                                                                            >  1:1090818/1‑150 (MQ=255)
                        cccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAat                                                                                                                           >  2:1526208/1‑150 (MQ=255)
                         ccGCGTGCGTATTGGCGAGTACGTTATCGATATTCTCGACGTACAGGAAAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATGCGAATGCCGGGCCGttttcg                                                                                                                          <  2:823509/150‑6 (MQ=255)
                         ccGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAatt                                                                                                                          <  2:1656500/150‑1 (MQ=255)
                          cgcgTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCTGCATTCGCATGCAGGGCCGTGAatta                                                                                                                         >  1:293598/1‑150 (MQ=255)
                           gcgtgcgtATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAattat                                                                                                                        <  1:429313/150‑1 (MQ=255)
                                      ggCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACAGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAg                                                                                                             >  1:1080484/1‑150 (MQ=255)
                                        cGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCTGAGTATCGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAgca                                                                                                           >  2:1113720/1‑148 (MQ=255)
                                        cGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGaa                                                                                                           <  2:309127/150‑1 (MQ=255)
                                        cGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGaa                                                                                                           >  1:917313/1‑150 (MQ=255)
                                           gTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAAtt                                                                                                        >  2:1368417/1‑150 (MQ=255)
                                             aCGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTaa                                                                                                      <  2:1712426/150‑1 (MQ=255)
                                              cgatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAg                                                                                                     >  1:942499/1‑150 (MQ=255)
                                               gatatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGc                                                                                                    <  1:387698/150‑1 (MQ=255)
                                                atatcgatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCt                                                                                                   >  2:336122/1‑150 (MQ=255)
                                                     gatatTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGc                                                                                              <  1:512777/150‑1 (MQ=255)
                                                        atTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCttt                                                                                           <  1:1619935/150‑1 (MQ=255)
                                                        atTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCttt                                                                                           <  1:1189051/150‑1 (MQ=255)
                                                          tctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTGTTGCTTTcc                                                                                         >  2:327597/1‑149 (MQ=255)
                                                          tctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAAAAGCTTAATTTCTGCTTTCg                                                                                         <  1:778646/150‑1 (MQ=255)
                                                          tctcGACGTACACGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAAAAGCTTAATTTCTGCTTTCg                                                                                         <  1:39192/150‑1 (MQ=255)
                                                           ctcGACGTACAGGACAATATGATTCAGCATGTAAAACTTTTTCCTGTTAAACCGCTGCGCGAGAGTGTGGCTCAGTAACGAAAACGGCCCGGCATTCGCATTCAGGGCCGTGAATAATTACGAAATCAGAAATTAAGCTTTTGCTTTCGc                                                                                        <  1:912445/150‑1 (MQ=255)
                                                           ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTTAGCTTTTGCTTTCgg                                                                                        >  2:277102/1‑149 (MQ=255)
                                                           ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                        <  1:948192/150‑1 (MQ=255)
                                                           ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                        >  1:492941/1‑150 (MQ=255)
                                                           ctcGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGc                                                                                        >  2:1424110/1‑150 (MQ=255)
                                                             cGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTTGCTa                                                                                      <  2:135652/150‑1 (MQ=255)
                                                             cGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTa                                                                                      >  2:1477804/1‑150 (MQ=255)
                                                             cGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTa                                                                                      >  2:35434/1‑150 (MQ=255)
                                                              gccggaCAGGACAATATGATTAAGCAGTTAAACCTATTTCCTGTGACACCGCTGCGCGAGAGTGAGGCGGAGTAACGAAAACGGCCCGGCAGTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCGTTCGCTAc                                                                                     <  1:277102/145‑1 (MQ=255)
                                                              gACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACTAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAAAAGCTTAATTTCTGCTTTCgtaat                                                                                     <  1:1478239/150‑5 (MQ=255)
                                                               aCGTACAGGACAATATGATTAAGCAGGTAAGAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACa                                                                                    >  1:685389/1‑150 (MQ=255)
                                                                cGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAg                                                                                   <  2:1090818/150‑1 (MQ=255)
                                                                cGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAg                                                                                   >  1:365614/1‑150 (MQ=255)
                                                                cGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGACCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAg                                                                                   <  2:1355068/150‑1 (MQ=255)
                                                                  tACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGGGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTa                                                                                 <  2:548095/150‑1 (MQ=255)
                                                                      ggACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCa                                                                             <  2:146618/150‑1 (MQ=255)
                                                                       gACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAt                                                                            >  2:781950/1‑150 (MQ=255)
                                                                       gACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGAATGCAGGGCCGTGAATTATTACGAAAGAAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCAt                                                                            >  1:536251/1‑150 (MQ=255)
                                                                          aaTATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATcgc                                                                         <  1:781950/150‑1 (MQ=255)
                                                                              tGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCa                                                                     >  1:1383499/1‑150 (MQ=255)
                                                                                aTTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                   >  2:1264803/1‑150 (MQ=255)
                                                                                aTTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACg                                                                   <  1:35434/150‑1 (MQ=255)
                                                                                      cAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGt                                                             >  1:608897/1‑150 (MQ=255)
                                                                                      cAGGTAAAAGTTTGTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGt                                                             <  1:1516917/150‑1 (MQ=255)
                                                                                          tAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGa                                                         <  1:1526208/150‑1 (MQ=255)
                                                                                            aaaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAcc                                                       >  2:1414708/1‑150 (MQ=255)
                                                                                            aaaGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGAcc                                                       <  2:1080484/150‑1 (MQ=255)
                                                                                              aGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCAt                                                     <  1:56967/150‑1 (MQ=255)
                                                                                               gTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCAtt                                                    >  1:96033/1‑150 (MQ=255)
                                                                                                tttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTc                                                   <  2:1553313/150‑1 (MQ=255)
                                                                                                tttttCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTc                                                   <  2:685389/150‑1 (MQ=255)
                                                                                                  tttCCTGTGAATCCGCTGCGCGTGAGTGTGGCGGAGTTACGTAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAg                                                 <  2:122585/150‑1 (MQ=255)
                                                                                                     ccTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGt                                              >  1:172802/1‑150 (MQ=255)
                                                                                                       tgtgAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGtat                                            >  1:615929/1‑150 (MQ=255)
                                                                                                             aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                      >  2:8994/1‑150 (MQ=255)
                                                                                                             aCCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCt                                      >  2:42495/1‑150 (MQ=255)
                                                                                                                 cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTg                                  <  2:1038600/150‑1 (MQ=255)
                                                                                                                 cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTg                                  <  2:892906/150‑1 (MQ=255)
                                                                                                                 cTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTg                                  <  2:886900/150‑1 (MQ=255)
                                                                                                                     gcgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCCTCGCCGCACGAATCGTACGACCATTCCGCCTAGAACCCTTCTGCATa                              >  2:259194/1‑150 (MQ=255)
                                                                                                                     gcgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATa                              <  1:730568/150‑1 (MQ=255)
                                                                                                                      cgAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATaa                             >  1:1261543/1‑150 (MQ=255)
                                                                                                                         gagTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGc                          <  2:917313/150‑1 (MQ=255)
                                                                                                                           gtgtGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGccc                        <  2:738464/150‑1 (MQ=255)
                                                                                                                             gtgGCGGAGTAACGAAATCGGCACGTCATTCGCATGGAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTTCAGTAACCATCGCCGCACGAATCGTACGACCATTCCGCGTATATCCCTTCTGCATAATGCTCAg                      <  1:287048/150‑1 (MQ=255)
                                                                                                                             gtgGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAg                      <  2:1659135/150‑1 (MQ=255)
                                                                                                                              tgGCGGAGTAACGCAACCGGCCCGGCCGTCGCCTGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTCACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGt                     <  2:260214/150‑1 (MQ=255)
                                                                                                                                         cGAAAACGGCCCGGCATTCTCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCAATCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTgg          <  1:102689/150‑1 (MQ=255)
                                                                                                                                         cGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGAAGAAATTAAGCTTTTGCTTTCGATACAGTAACCATCGCCGCCCGAATCGTACGACCATTCAGAGTATAACCATTCTGCATAATGCCAAGTACGTTACCggg          >  1:1081697/1‑150 (MQ=255)
                                                                                                                                           aaaaCGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTATGTTACCTGgcg        <  2:172802/150‑1 (MQ=255)
                                                                                                                                             caCGGCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCTTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCa      >  2:843518/2‑150 (MQ=255)
                                                                                                                                             caCGGCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCa      >  1:586972/2‑150 (MQ=255)
                                                                                                                                             caCGGCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCa      >  2:1142351/2‑150 (MQ=255)
                                                                                                                                             caCGGCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCa      >  2:604165/2‑150 (MQ=255)
                                                                                                                                             caCGGCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCa      >  1:190896/2‑150 (MQ=255)
                                                                                                                                                ggCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACg   >  1:1180602/1‑150 (MQ=255)
                                                                                                                                                ggCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACg   >  1:1312337/1‑150 (MQ=255)
                                                                                                                                                ggCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACg   <  1:1424110/150‑1 (MQ=255)
                                                                                                                                                ggCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACg   <  2:1383499/150‑1 (MQ=255)
                                                                                                                                                 gCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGt  >  2:48010/1‑150 (MQ=255)
                                                                                                                                                 gCCCTGCATGCGAATGCCGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGACCAGTACGTTACCTGGCGCAACGt  >  1:860526/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TGGAAGAGATCCCTGTCGCAGGCACCCGCGTGCGTATTGGCGAGTACGATATCGATATTCTCGACGTACAGGACAATATGATTAAGCAGGTAAAAGTTTTTCCTGTGAAACCGCTGCGCGAGAGTGTGGCGGAGTAACGAAAACGGCCCGGCATTCGCATGCAGGGCCGTGAATTATTACGAAAGCAGAAATTAAGCTTTTGCTTTCGCTACAGTAACCATCGCCGCACGAATCGTACGACCATTCAGCGTATAACCCTTCTGCATAATGCCCAGTACGTTACCTGGCGCAACGT  >  NC_000913/2749924‑2750218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: