Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,775,502 T→C 6.7% intergenic (+481/‑43) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,775,5020TC 6.7% 431.5 / 15.8 151intergenic (+481/‑43)yfjW/ypjICP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base T (67/72);  new base C (4/6);  total (73/78)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

Only 100 of 154 total aligned reads displayed.

CTGTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTATCAC  >  NC_000913/2775354‑2775651
                                                                                                                                                    |                                                                                                                                                     
cTGTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAAtctc                                                                                                                                                      <  2:92726/150‑1 (MQ=255)
cTGTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAAtctc                                                                                                                                                      >  1:1347258/1‑150 (MQ=255)
 tGTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCa                                                                                                                                                     <  2:83763/150‑1 (MQ=255)
 tGTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCa                                                                                                                                                     >  1:1150838/1‑150 (MQ=255)
  gTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAg                                                                                                                                                    >  1:421585/1‑150 (MQ=255)
  gTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCCGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAg                                                                                                                                                    <  1:1061074/150‑1 (MQ=255)
   tAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAgg                                                                                                                                                   <  1:1556253/150‑1 (MQ=255)
        gCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCt                                                                                                                                              >  2:1129859/1‑150 (MQ=255)
         ccccTACAAAATGAACCTTTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTg                                                                                                                                             <  1:653637/150‑1 (MQ=255)
          cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGc                                                                                                                                            >  1:1238032/1‑150 (MQ=255)
          cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGc                                                                                                                                            <  1:730189/150‑1 (MQ=255)
          cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGc                                                                                                                                            >  1:578244/1‑150 (MQ=255)
          cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGc                                                                                                                                            <  1:853058/150‑1 (MQ=255)
          cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAAACTCAGGATTCTGc                                                                                                                                            <  2:359810/150‑1 (MQ=255)
           ccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGcc                                                                                                                                           <  2:1709295/150‑1 (MQ=255)
            cTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGCCg                                                                                                                                          <  1:1253409/150‑1 (MQ=255)
             tACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGCCgt                                                                                                                                         <  1:1618209/150‑2 (MQ=255)
               cAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACATCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtt                                                                                                                                       >  2:973294/1‑150 (MQ=255)
               aaaaaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtt                                                                                                                                       <  1:947182/149‑1 (MQ=255)
                aaaaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCttt                                                                                                                                      >  2:538312/1‑150 (MQ=255)
                 aaaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtttt                                                                                                                                     >  2:699161/1‑150 (MQ=255)
                 aaaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtttt                                                                                                                                     >  1:2918/1‑150 (MQ=255)
                  aaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGCCgttaatc                                                                                                                                    <  1:777429/150‑7 (MQ=255)
                   aTGATCCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtttttt                                                                                                                                   <  1:36013/150‑1 (MQ=255)
                   aTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtttttt                                                                                                                                   <  1:699161/150‑1 (MQ=255)
                      aaCCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACTGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTttatt                                                                                                                                >  2:1081208/1‑150 (MQ=255)
                         cATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTattatt                                                                                                                             >  1:1164152/1‑150 (MQ=255)
                             attTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTc                                                                                                                         >  2:564117/1‑150 (MQ=255)
                               ttttAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAg                                                                                                                       <  2:793702/150‑1 (MQ=255)
                               ttttAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAg                                                                                                                       <  2:1435196/150‑1 (MQ=255)
                                tttAGATGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAgg                                                                                                                      <  2:1407105/150‑1 (MQ=255)
                                    gagGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGag                                                                                                                  <  2:1175550/150‑1 (MQ=255)
                                             tGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGa                                                                                                         <  2:586213/150‑1 (MQ=255)
                                              ggCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAg                                                                                                        <  2:612822/150‑1 (MQ=255)
                                               gCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGc                                                                                                       <  1:1651144/150‑1 (MQ=255)
                                                cACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAACCATTCAGATTTATATCATTTCTACAAACATAACCGATTAACTGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGTGAGTAGTAATGAGCa                                                                                                      <  2:1036193/150‑1 (MQ=255)
                                                     cAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAActct                                                                                                 >  2:654045/1‑150 (MQ=255)
                                                     cAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAAc                                                                                                    <  1:1409087/147‑1 (MQ=255)
                                                     cAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAAc                                                                                                    >  2:1409087/1‑147 (MQ=255)
                                                           ccGCTTTTGGCACAGAACGGCCGATCAGCAATATAAAAGCATTCTGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGt                                                                                           <  1:242095/150‑1 (MQ=255)
                                                                  tGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGt                                                                                    >  1:1187537/1‑150 (MQ=255)
                                                                   ggCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTc                                                                                   >  2:409468/1‑150 (MQ=255)
                                                                    gCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTTGTATTCa                                                                                  >  1:1205984/1‑150 (MQ=255)
                                                                     cacaGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACACGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAt                                                                                 >  2:839896/1‑150 (MQ=255)
                                                                        aGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtttt                                                                              <  2:50352/150‑1 (MQ=255)
                                                                        aGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtttt                                                                              <  1:1293429/150‑1 (MQ=255)
                                                                        aGAAAGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtttt                                                                              <  2:451379/150‑1 (MQ=255)
                                                                         gAACGGCCTATCATCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTa                                                                             <  1:1713213/150‑1 (MQ=255)
                                                                         gAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTa                                                                             <  2:1610698/150‑1 (MQ=255)
                                                                         gAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTa                                                                             >  2:1359873/1‑150 (MQ=255)
                                                                          aaCGTCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAg                                                                            <  2:1314465/150‑1 (MQ=255)
                                                                          aaCGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAg                                                                            <  2:1552216/150‑1 (MQ=255)
                                                                            cGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCa                                                                          <  2:2918/150‑1 (MQ=255)
                                                                             gcccTATCAGCAAAATAACAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCaa                                                                         <  2:1503161/148‑1 (MQ=255)
                                                                                cTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACg                                                                      >  2:1650846/1‑150 (MQ=255)
                                                                                      gCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtt                                                                                >  1:913679/1‑134 (MQ=255)
                                                                                      gCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCt                                                                <  2:1150838/150‑1 (MQ=255)
                                                                                           ataAAAGCATTCAGATATATATCATTTATACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGGCATTTTAGCAAACGCTTTCTGGTCt                                                           <  1:219975/150‑1 (MQ=255)
                                                                                            taAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTg                                                          >  2:690642/1‑150 (MQ=255)
                                                                                             aaaaGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGc                                                         <  1:1129859/150‑1 (MQ=255)
                                                                                              aaaGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTATGCCGCTTTTTTATTTTTTTTGAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTATCAAACGCTTTCTGGTCTGcc                                                        >  1:295850/1‑150 (MQ=255)
                                                                                                aGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGc                                                      <  2:520460/150‑1 (MQ=255)
                                                                                                   aTTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGt                                                   >  2:365189/1‑150 (MQ=255)
                                                                                                    ttCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTg                                                  >  1:758821/1‑150 (MQ=255)
                                                                                                    ttCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTg                                                  >  1:1707135/1‑150 (MQ=255)
                                                                                                    ttCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTATCCGGTCTGCCGCAGTg                                                  >  2:1375785/1‑150 (MQ=255)
                                                                                                     tCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTgg                                                 <  2:1164152/150‑1 (MQ=255)
                                                                                                       ctttatatataATTTAGACAAAACTACCCGCTTTTCGGGCGTCTCTAAGGCTTCTGCCGCTTTTTTATTATTTTTCCGGGGAGTATGAATGAGCAACTCTGAATGGTTGTATTCATTTTATCAAACGCGTTCTGGGCTGCCGCCGGGGGc                                               <  2:224592/147‑1 (MQ=255)
                                                                                                       aGATATATATCATTTCTACAAACATACCCGATTAACGACAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGTGc                                               >  2:169475/1‑150 (MQ=255)
                                                                                                        tatatatatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCa                                              <  1:389778/149‑1 (MQ=255)
                                                                                                           atatatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCg                                           <  1:1443605/150‑1 (MQ=255)
                                                                                                              tatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCt                                        >  1:1336466/1‑150 (MQ=255)
                                                                                                              tatCATTTCTAAAAACATACCCGATTAACGGCAGAATCTCAGGTTTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCt                                        <  2:141503/150‑1 (MQ=255)
                                                                                                                tCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGa                                      <  1:420550/150‑1 (MQ=255)
                                                                                                                tCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGa                                      >  1:577500/1‑150 (MQ=255)
                                                                                                                  aTTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGAtt                                    >  1:1661830/1‑150 (MQ=255)
                                                                                                                        aCAAACATAGCCGATTAACGGCAGAATCTGAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAAGTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAg                              <  2:111152/150‑1 (MQ=255)
                                                                                                                        aCAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAg                              >  1:538536/1‑150 (MQ=255)
                                                                                                                           aaCATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAg                           >  1:962200/1‑150 (MQ=255)
                                                                                                                                aCCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTGGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCa                      >  2:305708/1‑150 (MQ=255)
                                                                                                                                 cccGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCAc                     >  1:1549954/1‑150 (MQ=255)
                                                                                                                                 cccGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCAc                     >  2:410203/1‑150 (MQ=255)
                                                                                                                                 cccGATTAACGGCAGAATCTCAGGATTCTACCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCAc                     >  1:1007711/1‑150 (MQ=255)
                                                                                                                                 cccGATTAACGGCAGAATCACAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCAc                     >  2:1010338/1‑150 (MQ=255)
                                                                                                                                    gATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTgg                  <  2:451063/150‑1 (MQ=255)
                                                                                                                                     aTTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGt                 <  2:740194/150‑1 (MQ=255)
                                                                                                                                      ttAACGGCAGAATCTCAGGATTCAGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGGTGGTAGTCATTTTAGCACACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTa                <  1:230590/150‑1 (MQ=255)
                                                                                                                                        aaCGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATc              >  1:1086158/1‑150 (MQ=255)
                                                                                                                                         aCGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCt             <  2:1347258/150‑1 (MQ=255)
                                                                                                                                          cGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTg            >  1:71817/1‑150 (MQ=255)
                                                                                                                                          cGGCAGAATCCTGAGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTg            >  2:557706/1‑150 (MQ=255)
                                                                                                                                          cGGCAGAATCCTGAGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTg            >  2:962127/1‑150 (MQ=255)
                                                                                                                                           ggCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGc           >  2:136365/1‑150 (MQ=255)
                                                                                                                                           ggCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGc           <  2:578244/150‑1 (MQ=255)
                                                                                                                                           ggCAGAATCCTGAGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGc           >  1:4661/1‑150 (MQ=255)
                                                                                                                                              aGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCgg        <  1:69066/150‑1 (MQ=255)
                                                                                                                                              aGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCgg        <  1:1354143/150‑1 (MQ=255)
                                                                                                                                              aGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCgg        <  2:1205984/150‑1 (MQ=255)
                                                                                                                                              aGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCgg        <  1:1414909/150‑1 (MQ=255)
                                                                                                                                                    tcAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTATCAc  <  1:1302784/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTGTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTATCAC  >  NC_000913/2775354‑2775651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: