Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,775,505 G→A 6.7% intergenic (+484/‑40) yfjW → / → ypjI CP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,775,5050GA 6.7% 466.1 / 13.7 148intergenic (+484/‑40)yfjW/ypjICP4‑57 prophage; putative inner membrane protein/pseudogene, CP4‑57 putative prophage remnant;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (67/71);  new base A (4/6);  total (71/77)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.05e-01

Only 100 of 154 total aligned reads displayed.

GTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTATCACC  >  NC_000913/2775356‑2775652
                                                                                                                                                     |                                                                                                                                                   
gTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAg                                                                                                                                                     >  2:560492/1‑150 (MQ=255)
gTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAg                                                                                                                                                     >  1:421585/1‑150 (MQ=255)
gTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCCGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAg                                                                                                                                                     <  1:1061074/150‑1 (MQ=255)
      gCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCt                                                                                                                                               >  2:1129859/1‑150 (MQ=255)
       ccccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATAAACGGCAGAATCTCAGGATTCTg                                                                                                                                              >  2:609686/1‑150 (MQ=255)
        cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGc                                                                                                                                             >  1:1238032/1‑150 (MQ=255)
        cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGc                                                                                                                                             <  1:730189/150‑1 (MQ=255)
        cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGc                                                                                                                                             >  1:578244/1‑150 (MQ=255)
        cccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAAACTCAGGATTCTGc                                                                                                                                             <  2:359810/150‑1 (MQ=255)
         ccTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGcc                                                                                                                                            >  2:1414909/1‑150 (MQ=255)
          cTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGCCg                                                                                                                                           <  1:1253409/150‑1 (MQ=255)
           tACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGCCgt                                                                                                                                          <  2:584306/150‑2 (MQ=255)
           tACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGCCgt                                                                                                                                          <  1:1618209/150‑2 (MQ=255)
             cAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACATCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtt                                                                                                                                        >  2:973294/1‑150 (MQ=255)
              aaaaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCttt                                                                                                                                       >  2:538312/1‑150 (MQ=255)
               aaaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtttt                                                                                                                                      >  2:699161/1‑150 (MQ=255)
                aaTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCCTGAGATTCTGCCgttaatc                                                                                                                                     <  1:777429/150‑7 (MQ=255)
                 aTGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCtttttt                                                                                                                                    <  1:699161/150‑1 (MQ=255)
                   gAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTttat                                                                                                                                  >  2:431115/1‑150 (MQ=255)
                    aaCCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACTGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTttatt                                                                                                                                 >  2:1081208/1‑150 (MQ=255)
                    aaCCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTttatt                                                                                                                                 <  2:665522/150‑1 (MQ=255)
                       cATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTattatt                                                                                                                              >  2:219975/1‑150 (MQ=255)
                       cATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTattatt                                                                                                                              >  1:1164152/1‑150 (MQ=255)
                           attTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTc                                                                                                                          >  2:564117/1‑150 (MQ=255)
                           attTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTc                                                                                                                          <  1:407309/150‑1 (MQ=255)
                             ttttAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAg                                                                                                                        >  1:740194/1‑150 (MQ=255)
                             ttttAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAg                                                                                                                        <  2:793702/150‑1 (MQ=255)
                              tttAGATGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAgg                                                                                                                       <  2:1407105/150‑1 (MQ=255)
                               ttAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAggg                                                                                                                      <  2:1573742/150‑1 (MQ=255)
                                        gTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTaa                                                                                                             >  1:451063/1‑150 (MQ=255)
                                           tGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGa                                                                                                          >  1:586213/1‑150 (MQ=255)
                                            ggCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAg                                                                                                         <  2:612822/150‑1 (MQ=255)
                                                 tCCAACTTCCGCTTTTGGCACAGCACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTCCAAACATACCCGATTAACGGCAGAATCTAAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAAct                                                                                                    >  2:80913/1‑150 (MQ=255)
                                                       ttCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAg                                                                                              >  2:1174087/1‑150 (MQ=255)
                                                       ttCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAg                                                                                              <  2:1477768/150‑1 (MQ=255)
                                                         ccGCTTTTGGCACAGAACGGCCGATCAGCAATATAAAAGCATTCTGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGt                                                                                            <  1:242095/150‑1 (MQ=255)
                                                          cGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGtt                                                                                           >  1:1555606/1‑150 (MQ=255)
                                                              tttGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTa                                                                                       <  1:560492/150‑1 (MQ=255)
                                                                tGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGt                                                                                     >  1:1187537/1‑150 (MQ=255)
                                                                tGGAACAGAACGGCCTATCAGAAATATAAAAGCATTCAGATATATATCATATCTACAAACAAACCCGATTAACGGCAGAATCTCAGCATTCTGCCGCTTTTTTAATATTATTCAGGGGAGAAGTAATGATAAACTCTGAAGGTTagtagt                                                                                     >  1:703336/1‑150 (MQ=255)
                                                                 ggCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTc                                                                                    >  2:409468/1‑150 (MQ=255)
                                                                  gCACAGAACGGCCTATCAGCAATATTAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCa                                                                                   >  1:935541/1‑150 (MQ=255)
                                                                  gCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTTGTATTCa                                                                                   >  1:1205984/1‑150 (MQ=255)
                                                                      aGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGTATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtttt                                                                               <  1:195128/150‑1 (MQ=255)
                                                                      aGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtttt                                                                               <  2:317169/150‑1 (MQ=255)
                                                                      aGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtttt                                                                               <  1:1293429/150‑1 (MQ=255)
                                                                      aGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtttt                                                                               <  2:50352/150‑1 (MQ=255)
                                                                       gAACGGCCTATCATCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTa                                                                              <  1:1713213/150‑1 (MQ=255)
                                                                       gAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTa                                                                              <  2:1610698/150‑1 (MQ=255)
                                                                       gAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTa                                                                              >  2:1359873/1‑150 (MQ=255)
                                                                        aaCGTCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAg                                                                             <  2:1314465/150‑1 (MQ=255)
                                                                           gcccTATCAGCAAAATAACAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCaa                                                                          <  2:1503161/148‑1 (MQ=255)
                                                                                   aGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTc                                                                  >  2:1675358/1‑150 (MQ=255)
                                                                                   aGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTc                                                                  <  2:1089428/150‑1 (MQ=255)
                                                                                    gCAATATAAAAGCATTCAGATATATATCATTTCTATAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCt                                                                 >  2:524877/1‑150 (MQ=255)
                                                                                    gCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtt                                                                                 >  1:913679/1‑134 (MQ=255)
                                                                                    gCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCAtt                                                                                 <  2:913679/134‑1 (MQ=255)
                                                                                     cAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCTGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTg                                                                <  1:851675/150‑1 (MQ=255)
                                                                                      aaTATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTgg                                                               <  1:968899/150‑1 (MQ=255)
                                                                                       atatAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCGCAGGATTCTGCCGCTTTTTTATTATTATTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCCAACGCTTTCTGGt                                                              >  1:224592/1‑150 (MQ=255)
                                                                                           aaaaGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGc                                                          <  1:1129859/150‑1 (MQ=255)
                                                                                            aaaGCATTCAGATATATATCATTTCTACAAACATACCCTATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGcc                                                         <  1:143294/150‑1 (MQ=255)
                                                                                            aaaGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTATGCCGCTTTTTTATTTTTTTTGAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTATCAAACGCTTTCTGGTCTGcc                                                         >  1:295850/1‑150 (MQ=255)
                                                                                             aaGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCg                                                        <  1:1675358/150‑1 (MQ=255)
                                                                                               gCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCa                                                      >  2:294623/1‑150 (MQ=255)
                                                                                                  ttCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTATCCGGTCTGCCGCAGTg                                                   >  2:1375785/1‑150 (MQ=255)
                                                                                                   tCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTgg                                                  <  2:1164152/150‑1 (MQ=255)
                                                                                                    taGATATATATCATTTCTACAAAGATTCCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTTGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTggg                                                 <  2:703336/149‑1 (MQ=255)
                                                                                                     ctttatatataATTTAGACAAAACTACCCGCTTTTCGGGCGTCTCTAAGGCTTCTGCCGCTTTTTTATTATTTTTCCGGGGAGTATGAATGAGCAACTCTGAATGGTTGTATTCATTTTATCAAACGCGTTCTGGGCTGCCGCCGGGGGc                                                <  2:224592/147‑1 (MQ=255)
                                                                                                     aGATATATATCATTTCTACAAACATACCCGATTAACGACAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGTGc                                                >  2:169475/1‑150 (MQ=255)
                                                                                                      tatatatatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCa                                               <  1:389778/149‑1 (MQ=255)
                                                                                                        tatatatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATc                                             <  1:1650846/150‑1 (MQ=255)
                                                                                                           atatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGc                                          >  2:407498/1‑150 (MQ=255)
                                                                                                            tatCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCt                                         >  1:1336466/1‑150 (MQ=255)
                                                                                                            tatCATTTCTAAAAACATACCCGATTAACGGCAGAATCTCAGGTTTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCt                                         <  2:141503/150‑1 (MQ=255)
                                                                                                             atCATTTCTACAAACATACGCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTg                                        <  2:491357/150‑1 (MQ=255)
                                                                                                              tCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTAATTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGAAAACGCTTTCTGGTCTGCCGCAGTGGGCTTCGGCTGa                                       >  1:317716/1‑150 (MQ=255)
                                                                                                               cATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGAt                                      <  2:1132563/150‑1 (MQ=255)
                                                                                                                aTTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGAtt                                     >  1:1661830/1‑150 (MQ=255)
                                                                                                                      aCAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAg                               >  1:538536/1‑150 (MQ=255)
                                                                                                                         aaCATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAg                            >  1:962200/1‑150 (MQ=255)
                                                                                                                           aaTACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAggg                          <  2:1019914/149‑1 (MQ=255)
                                                                                                                               cccGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCAc                      >  1:1549954/1‑150 (MQ=255)
                                                                                                                               cccGATTAACGGCAGAATCTCAGGATTCTACCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCAc                      >  1:1007711/1‑150 (MQ=255)
                                                                                                                                  gATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTgg                   <  2:451063/150‑1 (MQ=255)
                                                                                                                                  gATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTgg                   >  1:676810/1‑150 (MQ=255)
                                                                                                                                      aaCGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATc               >  1:1086158/1‑150 (MQ=255)
                                                                                                                                       aCGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCt              <  2:1347258/150‑1 (MQ=255)
                                                                                                                                        cGGCAGAATCTCAGGATTCTGCCGCTTTTTTATAATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGTGTAGCAGGGCCACTGGTATCTg             >  1:251621/1‑150 (MQ=255)
                                                                                                                                        cGGCAGAATCCTGAGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTg             >  2:962127/1‑150 (MQ=255)
                                                                                                                                         ggCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGc            >  2:136365/1‑150 (MQ=255)
                                                                                                                                            aGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCgg         <  2:1205984/150‑1 (MQ=255)
                                                                                                                                            aGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCgg         <  1:1354143/150‑1 (MQ=255)
                                                                                                                                            aGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCgg         <  1:1414909/150‑1 (MQ=255)
                                                                                                                                             gAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGt        <  1:1398823/150‑1 (MQ=255)
                                                                                                                                                  tcAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTATCAc   <  1:1302784/150‑1 (MQ=255)
                                                                                                                                                   cAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTATCAcc  <  2:1402493/150‑1 (MQ=255)
                                                                                                                                                   cAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGGTGGTAGTCATTTTAGCAATCGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTATCAcc  >  2:664018/1‑150 (MQ=255)
                                                                                                                                                     ggATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACt                     <  1:1185447/129‑1 (MQ=255)
                                                                                                                                                     ggATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACt                     >  2:1185447/1‑129 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTAAATGCCCCTACAAAATGAACCATTATTTTTAGAGGGCGTCTGGCACTCCAACTTCCGCTTTTGGCACAGAACGGCCTATCAGCAATATAAAAGCATTCAGATATATATCATTTCTACAAACATACCCGATTAACGGCAGAATCTCAGGATTCTGCCGCTTTTTTATTATTTTTCAGGGGAGTAGTAATGAGCAACTCTGAAGGTTGGTAGTCATTTTAGCAAACGCTTTCTGGTCTGCCGCAGTGGGCATCGGCTGATTGCGTAGCAGGGCCACTGGTATCTGCCGGTATCACC  >  NC_000913/2775356‑2775652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: