Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,802,659 G→A 10.1% A438T (GCC→ACC)  nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,802,6590GA10.1% 284.3 / 15.5 99A438T (GCC→ACC) nrdEribonucleoside‑diphosphate reductase 2, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (50/39);  new base A (4/6);  total (54/45)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

CTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATGGTTCG  >  NC_000913/2802511‑2802808
                                                                                                                                                    |                                                                                                                                                     
cTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGc                                                                                                                                                      >  2:512560/1‑150 (MQ=255)
  cAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACAATGGATTCCCCCGATTTTGCCAGCACGGTAGAGACTGCCg                                                                                                                                                    >  1:66689/1‑150 (MQ=255)
    gAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTg                                                                                                                                                  <  1:1446707/150‑1 (MQ=255)
    gAAATTTTGCAGGTTAACAGCGCCTCAGAGAATGACGAGAATCGCGACTATACCCGCACAGGCCATGATATTTCGTGCAATTTAGGTTGGTTGAATATTGCGCACACCATGGATTCCCGCGATTTTGCCCGCACGGTAGAGACTGCCGTg                                                                                                                                                  >  2:1625776/1‑150 (MQ=255)
       aTTTTGCAGGTTCACTGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTCAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGCAGTCTCTACCGTgcgg                                                                                                                                               <  1:325491/150‑2 (MQ=255)
       aTTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGCAGTCTCTACCGTgcgg                                                                                                                                               <  2:430441/150‑2 (MQ=255)
       aTTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGCAGTCTCTACCGTgcgg                                                                                                                                               <  1:1410269/150‑2 (MQ=255)
        ttttGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGCAGTCTCTACCGTgcggg                                                                                                                                              <  2:877809/150‑3 (MQ=255)
        ttttGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGCAGTCTCTACCGTgcggg                                                                                                                                              <  1:716657/150‑3 (MQ=255)
         tttGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCgg                                                                                                                                             >  2:478316/1‑150 (MQ=255)
          ttGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGt                                                                                                                                            <  2:731995/150‑1 (MQ=255)
          ttGCAGGTTAACAGCGCCTCAGAGTATGACGAGAAGCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGt                                                                                                                                            <  1:997544/150‑1 (MQ=255)
           tGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGCAGTCTCTACCGTgcgggcaa                                                                                                                                           <  1:34680/150‑6 (MQ=255)
            gCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGttt                                                                                                                                          <  1:17773/150‑1 (MQ=255)
             cAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTa                                                                                                                                         >  2:160912/1‑150 (MQ=255)
               tgTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCACGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                       >  1:983503/2‑150 (MQ=255)
               ggTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                       <  1:685741/150‑1 (MQ=255)
               ggTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCACCCGATTTTGCACGCACGGTAGAGACTGCCGTGCGCGGTTTAAc                                                                                                                                       >  2:819805/1‑150 (MQ=255)
                  tAACAGCGCCTCAGAGTATTACGAGTATCTCGAGTATACCGGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGc                                                                                                                                    <  1:98502/150‑1 (MQ=255)
                  tAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATTTTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGc                                                                                                                                    >  2:1029550/1‑150 (MQ=255)
                    aCAGCGCCTCAGAGTTTGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAg                                                                                                                                  <  1:192539/150‑1 (MQ=255)
                     cAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGt                                                                                                                                 >  2:1658816/1‑150 (MQ=255)
                         gcCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCTCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCa                                                                                                                             <  2:983503/150‑1 (MQ=255)
                         gcCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCa                                                                                                                             >  1:757111/1‑150 (MQ=255)
                          ccTCAGAGTATGACGAGAATCTCGACTATACACGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAg                                                                                                                            >  1:1713679/1‑150 (MQ=255)
                             cAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGata                                                                                                                         <  2:1648746/150‑1 (MQ=255)
                               gagTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATg                                                                                                                       >  1:1001244/1‑150 (MQ=255)
                               gagTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATg                                                                                                                       <  2:295241/150‑1 (MQ=255)
                               gagTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATg                                                                                                                       >  1:1118078/1‑150 (MQ=255)
                                 gTATGTCGAGAATCACGACTCTACACGCAAAGGCCATGCTATTTCCTTCAATTTAGGTGCGGTGAATATTGCGCACCCCATTTATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGGGCGCGGTTTAACGGCAGTATCATATATGAg                                                                                                                     <  2:126085/150‑1 (MQ=255)
                                 gTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCAGTTTAACGGCAGTATCAGATATGAg                                                                                                                     <  2:1571164/150‑1 (MQ=255)
                                 gTAAGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAg                                                                                                                     <  2:1341808/150‑1 (MQ=255)
                                   aTGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTc                                                                                                                   >  2:352963/1‑150 (MQ=255)
                                    tGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATCTTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGGTTAACGGCAGTATCAGATATGAGTCa                                                                                                                  <  2:229510/150‑1 (MQ=255)
                                    tGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGTATTCCCCCGATTTTGCCCGCACGGGAGAGACTGCCGTGCGCGGTTTAACGGCAGTAGCAGATATGCGTCa                                                                                                                  >  1:329249/1‑150 (MQ=255)
                                    tGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCa                                                                                                                  <  2:774576/150‑1 (MQ=255)
                                    tGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCa                                                                                                                  >  2:1712684/1‑150 (MQ=255)
                                    tGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCa                                                                                                                  <  1:522904/150‑1 (MQ=255)
                                     gACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCat                                                                                                                 >  2:158867/1‑150 (MQ=255)
                                       cGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATTTGAGTCatat                                                                                                               >  2:1082523/1‑150 (MQ=255)
                                         agaATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATcc                                                                                                             >  2:10985/1‑150 (MQ=255)
                                          taaTCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTCGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCg                                                                                                            <  1:1333240/149‑1 (MQ=255)
                                                 gACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTg                                                                                                     <  2:65209/150‑1 (MQ=255)
                                                    tataCCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCg                                                                                                  >  2:60461/1‑150 (MQ=255)
                                                     ataCCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGt                                                                                                 >  2:626999/1‑150 (MQ=255)
                                                       aCCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTcc                                                                                               >  2:1416418/1‑150 (MQ=255)
                                                            cacaGGCCATGATATTTCGTGCTATTTAGGTTCGTTGAATATTGCGCACACCTTGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGa                                                                                          <  2:1161776/150‑1 (MQ=255)
                                                            cacaGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGa                                                                                          >  1:67368/1‑150 (MQ=255)
                                                             acaGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGAATTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGaa                                                                                         <  1:718847/150‑1 (MQ=255)
                                                                ggCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGcc                                                                                      <  2:1094130/150‑1 (MQ=255)
                                                                ggCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGcc                                                                                      >  1:1349607/1‑150 (MQ=255)
                                                                  ccATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCgg                                                                                    >  1:1324606/1‑150 (MQ=255)
                                                                         atTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATgcc                                                                             >  1:474626/1‑150 (MQ=255)
                                                                            tCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGCCCATCGAAGCCGGAAATgccgcc                                                                          <  2:1032065/150‑1 (MQ=255)
                                                                            tCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATATTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATgccgcc                                                                          >  1:424227/1‑150 (MQ=255)
                                                                             ccTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCt                                                                         >  1:833600/1‑150 (MQ=255)
                                                                               tGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCg                                                                       <  1:158867/150‑1 (MQ=255)
                                                                                 cAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCCCGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCa                                                                     <  1:1184931/150‑1 (MQ=255)
                                                                                              ttGAATATTCCGCACACCATGGATTCACCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTg                                                        <  1:819805/150‑1 (MQ=255)
                                                                                                 aaTATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTgggg                                                     >  1:392419/1‑150 (MQ=255)
                                                                                                 aaTATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTgggg                                                     >  1:1629433/1‑150 (MQ=255)
                                                                                                    atTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAg                                                  >  1:571243/1‑150 (MQ=255)
                                                                                                        cgcACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGa                                              <  1:1658816/150‑1 (MQ=255)
                                                                                                         gcACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGaa                                             <  2:67368/150‑1 (MQ=255)
                                                                                                          cacaCCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAAt                                            >  1:1666/1‑150 (MQ=255)
                                                                                                          cacaCCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAAt                                            >  2:543415/1‑150 (MQ=255)
                                                                                                            cacCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCCCGCCATCGGACTGGGGCAGATGAAttt                                          >  2:1049549/1‑150 (MQ=255)
                                                                                                            cacCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAAttt                                          <  1:1029550/150‑1 (MQ=255)
                                                                                                              ccATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTac                                        >  2:582198/1‑150 (MQ=255)
                                                                                                                aTGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTacac                                      >  2:1540648/1‑150 (MQ=255)
                                                                                                                 tGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACg                                     >  1:1567894/1‑150 (MQ=255)
                                                                                                                 tGGATTCCCCCGATTATGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCCGCGTGCCGTCCAGCGCAGCCGGAAATGACGACTCGCACGCCGTCGGAGTGGGGCTGATGAATTTacaag                                     >  2:355991/1‑148 (MQ=255)
                                                                                                                   gATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGc                                   >  1:1240131/1‑150 (MQ=255)
                                                                                                                    aTTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCt                                  <  2:1324606/150‑1 (MQ=255)
                                                                                                                    aTTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCt                                  <  2:1001244/150‑1 (MQ=255)
                                                                                                                       cccccGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATc                               >  1:1228717/1‑150 (MQ=255)
                                                                                                                         cccGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCCCGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTg                             >  2:1592694/1‑150 (MQ=255)
                                                                                                                           cGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGgc                           <  1:478316/150‑1 (MQ=255)
                                                                                                                           cGAATTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCTATGCCGGAAATGCCGCATCGCACGCCTTCGGACTGGGGCAGATGAATTTACACGGCTATCTGgc                           <  1:1668092/150‑1 (MQ=255)
                                                                                                                            gATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGgcg                          >  1:403194/1‑150 (MQ=255)
                                                                                                                             aTTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGgcgc                         >  2:1362336/1‑150 (MQ=255)
                                                                                                                              ttttGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGcgcg                        <  2:1349607/150‑1 (MQ=255)
                                                                                                                                ttGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCgag                      >  2:1495432/1‑150 (MQ=255)
                                                                                                                                 accgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGATTTTACACGGCTATCTGGCGCgaga                     >  1:5881/5‑150 (MQ=255)
                                                                                                                                 accgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCgaga                     >  2:1121572/5‑150 (MQ=255)
                                                                                                                                 accgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCgaga                     >  2:1580198/5‑150 (MQ=255)
                                                                                                                                 accgcGCACGGCAGTCTCTACCGTGCGCGGTTTAACGGCAGTATCAGATAAGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCgaga                     >  1:478782/5‑150 (MQ=255)
                                                                                                                                   cccGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAg                   >  1:371815/1‑150 (MQ=255)
                                                                                                                                   cccGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAg                   <  1:160912/150‑1 (MQ=255)
                                                                                                                                   cccGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAg                   >  1:856241/1‑150 (MQ=255)
                                                                                                                                   cccGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAg                   >  1:850584/1‑150 (MQ=255)
                                                                                                                                   cccGCACGGTAGAGACTGCCGTGAGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGTACTGGGGCAGATGAATTTACACGGCTATCTGCCGCGAGCAg                   >  1:1703807/1‑150 (MQ=255)
                                                                                                                                    ccGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAgg                  <  2:403194/150‑1 (MQ=255)
                                                                                                                                     cGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGc                 <  1:1335915/150‑1 (MQ=255)
                                                                                                                                        aCGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATc              <  1:352963/150‑1 (MQ=255)
                                                                                                                                               agaCTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATg       <  2:1629433/150‑1 (MQ=255)
                                                                                                                                                gaCTGCCGGGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATgg      <  2:534550/150‑1 (MQ=255)
                                                                                                                                                  cTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATGGtt    >  2:716044/1‑150 (MQ=255)
                                                                                                                                                  cTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATGGtt    >  1:1297117/1‑150 (MQ=255)
                                                                                                                                                    gCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATGGTTCg  <  2:833600/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CTCAGAAATTTTGCAGGTTAACAGCGCCTCAGAGTATGACGAGAATCTCGACTATACCCGCACAGGCCATGATATTTCCTGCAATTTAGGTTCGTTGAATATTGCGCACACCATGGATTCCCCCGATTTTGCCCGCACGGTAGAGACTGCCGTGCGCGGTTTAACGGCAGTATCAGATATGAGTCATATCCGCAGCGTGCCGTCCATCGAAGCCGGAAATGCCGCCTCGCACGCCATCGGACTGGGGCAGATGAATTTACACGGCTATCTGGCGCGAGAAGGCATCGCTTATGGTTCG  >  NC_000913/2802511‑2802808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: