Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,807,091 T→C 8.5% intergenic (+17/‑41) proW → / → proX glycine betaine/proline ABC transporter permease/glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,807,0910TC 8.5% 413.3 / 24.0 152intergenic (+17/‑41)proW/proXglycine betaine/proline ABC transporter permease/glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
Reads supporting (aligned to +/- strand):  ref base T (69/70);  new base C (2/11);  total (71/81)
Fisher's exact test for biased strand distribution p-value = 2.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.47e-03

Only 100 of 153 total aligned reads displayed.

CGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAG  >  NC_000913/2806942‑2807238
                                                                                                                                                     |                                                                                                                                                   
ggggATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGGCGCTGGTACACCACTGGCCCTGTTGGGCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGt                                                                                                                                                     <  2:180287/149‑1 (MQ=255)
  ggATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGCCCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTgc                                                                                                                                                   >  2:24817/1‑149 (MQ=255)
  ggATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGa                                                                                                                                                   <  2:400016/150‑1 (MQ=255)
   gATTGTGATCCTCGCTATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACCCCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGAc                                                                                                                                                  >  1:212332/1‑150 (MQ=255)
   gATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGAc                                                                                                                                                  >  1:1459507/1‑150 (MQ=255)
   gATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGAc                                                                                                                                                  >  1:1101515/1‑150 (MQ=255)
       gtgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCg                                                                                                                                              >  1:1204007/1‑150 (MQ=255)
          aTCCTCGCCATTATCCTCTATCGTCTGACGCAGGCCGTTGGTCGCGAGTCACGCAGTCGCGGCAACCGTCTCTTGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGc                                                                                                                                           <  2:60387/150‑1 (MQ=255)
           tCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCa                                                                                                                                          >  1:453192/1‑150 (MQ=255)
           tCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGGCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCa                                                                                                                                          <  1:1402877/150‑1 (MQ=255)
           cccccGCCATACTCCTCCCCCGCCTGACCCACGCCGCCGGGCGCGACTCACCCAGCCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTACCCGGCGTCACCGGGCa                                                                                                                                          <  2:1568052/146‑1 (MQ=255)
            ccTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCAt                                                                                                                                         >  1:157721/1‑150 (MQ=255)
             cTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCAtt                                                                                                                                        >  1:514809/1‑150 (MQ=255)
             cTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                                                                        <  1:421800/150‑3 (MQ=255)
             cTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                                                                        <  2:1707546/150‑3 (MQ=255)
             cTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                                                                        <  1:1650237/150‑3 (MQ=255)
             cTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                                                                        <  1:980570/150‑3 (MQ=255)
             cTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                                                                        <  1:960406/150‑3 (MQ=255)
             cTCGCCATTATCCTCGATCGTCTGACGCACGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCAtt                                                                                                                                        <  1:714560/150‑1 (MQ=255)
               ggCCATTATCCTCGATCGTCTGACGCAGGCCGTTTGGCGCGACTCACGCAGTCGCGGCAACCGACGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTAt                                                                                                                                      <  1:705583/149‑1 (MQ=255)
               cGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaagtg                                                                                                                                      <  2:453192/150‑5 (MQ=255)
                gCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATc                                                                                                                                     <  1:1481431/150‑1 (MQ=255)
                gCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATc                                                                                                                                     >  1:1261535/1‑150 (MQ=255)
                gCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaagtgc                                                                                                                                     <  1:1310946/150‑6 (MQ=255)
                gCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCa                                                                                                                                    <  2:1573033/150‑1 (MQ=255)
                 ccATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCTGTGACGCCGGGCATTATCa                                                                                                                                    <  2:1433211/150‑1 (MQ=255)
                   aTTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAcc                                                                                                                                  >  2:1411809/1‑150 (MQ=255)
                    ttATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAccc                                                                                                                                 <  1:824724/150‑1 (MQ=255)
                     tATCCTCGATCGTCTGACGCAGGCCGTTGGGTGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGACTGCTGACCCGCCCATTCATTAAGAAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCt                                                                                                                                >  2:381041/1‑150 (MQ=255)
                      aTCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTg                                                                                                                               <  2:1395018/150‑1 (MQ=255)
                         cTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCa                                                                                                                            <  2:270299/150‑1 (MQ=255)
                           cGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaa                                                                                                                          <  1:638264/150‑1 (MQ=255)
                                 tCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAgg                                                                                                                    >  1:1206533/1‑150 (MQ=255)
                                  cTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGCCGCCGGGCATTATCCGCCTGCCAAAAAAAGGa                                                                                                                   >  1:598573/1‑150 (MQ=255)
                                    gACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAAt                                                                                                                 <  2:1261535/150‑1 (MQ=255)
                                     aCGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATa                                                                                                                <  1:160334/150‑1 (MQ=255)
                                     aCGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATa                                                                                                                <  1:751395/150‑1 (MQ=255)
                                        cAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCAGTATCACCCTGCCAAAAAAAGGAATAACa                                                                                                             >  1:1493796/1‑150 (MQ=255)
                                          ggCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAAt                                                                                                           >  2:768684/1‑150 (MQ=255)
                                           gCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATg                                                                                                          <  2:1427535/150‑1 (MQ=255)
                                           gCCGGTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATg                                                                                                          <  1:249018/150‑1 (MQ=255)
                                               ttGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGAc                                                                                                      >  2:1626033/1‑150 (MQ=255)
                                                 gggCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACAt                                                                                                    <  1:1162972/150‑1 (MQ=255)
                                                   gcgcGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCAGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAg                                                                                                  <  2:816241/150‑1 (MQ=255)
                                                    cgcgACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGc                                                                                                 >  1:1261233/1‑150 (MQ=255)
                                                         cTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGCCCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACt                                                                                            >  1:1297945/1‑150 (MQ=255)
                                                         cTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCAGTAGGACTGCAGACCCGCCCATTCATAAAGTACCTCTGCCCTTGCCCGGTGACGCCGGGCAGTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACt                                                                                            >  2:1169813/1‑150 (MQ=255)
                                                           cACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACttt                                                                                          >  2:952681/1‑150 (MQ=255)
                                                           cACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACttt                                                                                          >  1:571618/1‑150 (MQ=255)
                                                              gCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTg                                                                                       <  2:782057/150‑1 (MQ=255)
                                                                 gTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTa                                                                                              >  2:660760/1‑140 (MQ=255)
                                                                  tCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGAc                                                                                   <  2:484215/150‑1 (MQ=255)
                                                                      ggCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCg                                                                               >  1:1409290/1‑150 (MQ=255)
                                                                       gCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGt                                                                              >  2:174958/1‑150 (MQ=255)
                                                                        cAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTTCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGtt                                                                             <  1:115986/150‑1 (MQ=255)
                                                                        cAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGtt                                                                             >  1:437157/1‑150 (MQ=255)
                                                                              tCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCAc                                                                       <  2:80798/150‑1 (MQ=255)
                                                                               cGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACg                                                                      >  2:1250027/1‑150 (MQ=255)
                                                                                   ggTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTa                                                                  >  1:1029643/1‑150 (MQ=255)
                                                                                   ggTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTa                                                                  <  1:1452568/150‑1 (MQ=255)
                                                                                   ggTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTa                                                                  <  1:1417171/150‑1 (MQ=255)
                                                                                   ggTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTa                                                                  <  1:620855/150‑1 (MQ=255)
                                                                                      acacCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGAAACTCTGCACTTGGCCGGTGACGCAGGGGTTTATCAGCCTGCCAAAAAAACGAATAACAATGCGACTTAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtct                                                               <  1:617319/150‑1 (MQ=255)
                                                                                        accacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATctct                                                             >  1:1210702/1‑150 (MQ=255)
                                                                                              ggCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACaa                                                       >  1:1099145/1‑150 (MQ=255)
                                                                                              ggCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTGTTTGCGACAGCGTTTGCCACGCTTATCTCTACACaa                                                       >  1:74192/1‑150 (MQ=255)
                                                                                               gCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTATCTCTGCACTTGCCCGGTGACGCCGGGCTTTATCACCCTGCCCAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTGATCGCTACACaaa                                                      >  1:1637671/1‑150 (MQ=255)
                                                                                                 ccTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACt                                                    <  1:174958/150‑1 (MQ=255)
                                                                                                  cTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATTACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACtt                                                   >  1:1694623/1‑150 (MQ=255)
                                                                                                        ggctgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgctg                                             <  1:1045829/148‑1 (MQ=255)
                                                                                                        gTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgctg                                             <  2:437157/150‑1 (MQ=255)
                                                                                                          ctgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGcc                                           <  2:1097169/150‑1 (MQ=255)
                                                                                                            gctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGa                                         >  2:1467839/1‑150 (MQ=255)
                                                                                                              tgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATc                                       <  2:403955/150‑1 (MQ=255)
                                                                                                               gACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCt                                      >  1:379601/1‑150 (MQ=255)
                                                                                                                aCCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAACCTTTTGCTGCCGATCTg                                     >  2:379225/1‑150 (MQ=255)
                                                                                                                 cccGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGc                                    >  1:646722/1‑150 (MQ=255)
                                                                                                                 cccGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATAACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGc                                    >  2:1198702/1‑150 (MQ=255)
                                                                                                                  ccGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGcc                                   >  2:623352/1‑150 (MQ=255)
                                                                                                                   cGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATACCCTGCCAAAAAAAGTAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCgg                                 >  1:56181/1‑150 (MQ=255)
                                                                                                                     cccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCGCCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCggg                                <  1:920711/150‑1 (MQ=255)
                                                                                                                     cccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCggg                                <  2:443193/150‑1 (MQ=255)
                                                                                                                      ccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGc                               <  2:1493796/150‑1 (MQ=255)
                                                                                                                       cattcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCa                              >  1:1681986/1‑150 (MQ=255)
                                                                                                                           cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAgg                          >  1:837992/1‑150 (MQ=255)
                                                                                                                           cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCAACACAAACTTTTGCTGCCGATCTGCCGGGCAAAgg                          >  2:373095/1‑150 (MQ=255)
                                                                                                                           cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGAAATTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGACGATCTGCCGGGCAAAgg                          >  2:464131/1‑150 (MQ=255)
                                                                                                                             ttAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCa                        <  2:1243630/150‑1 (MQ=255)
                                                                                                                                 gTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTAc                    >  1:200123/1‑150 (MQ=255)
                                                                                                                                  tAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACt                   >  1:1222255/1‑150 (MQ=255)
                                                                                                                                       ctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTaa              >  2:1204302/1‑150 (MQ=255)
                                                                                                                                       ctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTaa              <  1:603917/150‑1 (MQ=255)
                                                                                                                                          cACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATcc           <  2:139072/150‑1 (MQ=255)
                                                                                                                                             ttGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGt        <  2:360381/150‑1 (MQ=255)
                                                                                                                                             atGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGt        >  1:368481/2‑150 (MQ=255)
                                                                                                                                                cccGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCCACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCa     <  1:373095/150‑1 (MQ=255)
                                                                                                                                                 ccGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCag    <  2:1043130/150‑1 (MQ=255)
                                                                                                                                                 ccGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCag    <  2:1297945/150‑1 (MQ=255)
                                                                                                                                                  cGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCaga   <  2:571618/150‑1 (MQ=255)
                                                                                                                                                  cGGTGACGCCGGGAATTATAAACCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTATTCCAGTTCaga   >  2:1167096/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAG  >  NC_000913/2806942‑2807238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: