Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,837,471 C→T 5.1% intergenic (‑46/+107) hypF ← / ← hydN carbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein/formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,837,4710CT 5.1% 384.9 / 5.1 118intergenic (‑46/+107)hypF/hydNcarbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein/formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
Reads supporting (aligned to +/- strand):  ref base C (52/60);  new base T (4/2);  total (56/62)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.72e-01

Only 100 of 119 total aligned reads displayed.

TTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCA  >  NC_000913/2837325‑2837618
                                                                                                                                                  |                                                                                                                                                   
tttAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACg                                                                                                                                                  <  1:617952/150‑1 (MQ=255)
tttAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACg                                                                                                                                                  <  2:412854/150‑1 (MQ=255)
   aaTTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACa                                                                                                                                               >  1:1148861/1‑150 (MQ=255)
    aTTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAg                                                                                                                                              <  1:679099/150‑1 (MQ=255)
       gCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGt                                                                                                                                           >  1:470924/1‑150 (MQ=255)
         tgtgCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAATATGTGTGTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCg                                                                                                                                         <  1:83720/150‑1 (MQ=255)
                  ggCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGATGCGACGCTGTCGTGTCCGGCa                                                                                                                                <  2:1388424/149‑1 (MQ=255)
                   gCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAg                                                                                                                               >  1:219622/1‑150 (MQ=255)
                     cAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTc                                                                                                                             >  1:1583147/1‑150 (MQ=255)
                       gACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTACTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTcac                                                                                                                           >  1:16891/1‑150 (MQ=255)
                        aCAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGATGCGACGCTGTCGTGTCCGGCAgggcgg                                                                                                                          <  1:145781/150‑6 (MQ=38)
                          aaaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTGTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAgg                                                                                                                        <  1:70977/150‑1 (MQ=255)
                           aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                       >  2:944474/1‑150 (MQ=255)
                           aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                       <  1:670135/150‑1 (MQ=255)
                           aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                       >  2:420447/1‑150 (MQ=255)
                           aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                       >  2:1619984/1‑150 (MQ=255)
                              ggACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                    <  1:605474/150‑1 (MQ=255)
                              ggACGAAAACCGACGCCCTGCACTTTGCCACCAATACGCAGTGGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                    <  1:1537593/150‑1 (MQ=255)
                              ggACGAAAACCGACGACCTGAACTTTGCCACGAATACGCCGTTGGACACCGAAAGATGTGTTTTTTGCCATTGAGGTATTCCCGCCCTCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                    <  2:126446/150‑1 (MQ=255)
                               gACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGc                                                                                                                   <  1:891451/150‑1 (MQ=255)
                                aCGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCg                                                                                                                  <  2:1128192/150‑1 (MQ=255)
                                   aaaaCCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGCCACGCCCGCGTCGCATCCGGCAGGCACAGGTCGGCGAtc                                                                                                               >  1:1587314/1‑149 (MQ=255)
                                      aCCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccg                                                                                                            >  2:87901/1‑150 (MQ=255)
                                       ccGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgc                                                                                                           >  1:610217/1‑150 (MQ=255)
                                       ccGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgc                                                                                                           >  1:1427342/1‑150 (MQ=255)
                                           cGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgcccgc                                                                                                       >  1:1705033/1‑150 (MQ=255)
                                             cccTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTc                                                                                                     >  2:666286/1‑150 (MQ=255)
                                              ccTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTcc                                                                                                    <  1:343166/150‑1 (MQ=255)
                                               cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                   <  2:1521072/150‑1 (MQ=255)
                                               cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                   <  1:1272715/150‑1 (MQ=255)
                                               cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                   <  1:1104161/150‑1 (MQ=255)
                                                tGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGt                                                                                                  <  2:638553/150‑1 (MQ=255)
                                                 gCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTa                                                                                                 >  2:973578/1‑150 (MQ=255)
                                                       tGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTAc                                                                                           >  1:1437074/1‑150 (MQ=255)
                                                       tGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTAc                                                                                           >  1:977539/1‑150 (MQ=255)
                                                        gCCACGATTACGCATTTGGAAACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCTCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACg                                                                                          <  2:400532/150‑1 (MQ=255)
                                                        gCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACg                                                                                          <  1:945587/150‑1 (MQ=255)
                                                        gCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACg                                                                                          >  1:1621070/1‑150 (MQ=255)
                                                          cACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGaa                                                                                        <  1:722138/150‑1 (MQ=255)
                                                                   gCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCgg                                                                               <  2:1148861/150‑1 (MQ=255)
                                                                    cAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCggg                                                                              >  1:1173886/1‑150 (MQ=255)
                                                                    cAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCggg                                                                              <  1:648512/150‑1 (MQ=255)
                                                                    cAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCggg                                                                              >  1:827449/1‑150 (MQ=255)
                                                                     aGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGa                                                                             >  1:961251/1‑150 (MQ=255)
                                                                      gTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGaa                                                                            >  1:1521533/1‑150 (MQ=255)
                                                                         ggACACCGCAAGATGGGTGTTTTGCCATTGAGTTCTTCCCGCCATCATGAATTGCGTACCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTc                                                                         <  1:345141/150‑1 (MQ=255)
                                                                            cacCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCtt                                                                      >  2:1343069/1‑150 (MQ=255)
                                                                              ccGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTg                                                                    <  1:420447/150‑1 (MQ=255)
                                                                              ccGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTg                                                                    <  1:1619984/150‑1 (MQ=255)
                                                                               cGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGa                                                                   <  2:1583147/150‑1 (MQ=255)
                                                                                 cAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATg                                                                 <  2:1180888/150‑1 (MQ=255)
                                                                                 cAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGc                                                                <  1:1265694/150‑1 (MQ=255)
                                                                                   aGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGcc                                                               <  1:516541/150‑1 (MQ=255)
                                                                                   aGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGACGGACACGACAGCGTCGCATCCGGCAGGCCCAGGTAGGCGATACCGCCCGCTCCGTATTCTGAGGATATTTACGGGAATTCCTTTGATGcc                                                               >  1:1286052/1‑150 (MQ=255)
                                                                                     aTGTGGTTTTTGCCATTCAGTTATACCCGCCATCATGAATTGCGGAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAg                                                             <  2:1485458/150‑1 (MQ=255)
                                                                                        tgtTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAAc                                                          >  2:351382/1‑150 (MQ=255)
                                                                                         gtTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACa                                                         <  1:711341/150‑1 (MQ=255)
                                                                                           tttttGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGt                                                       <  2:610217/150‑1 (MQ=255)
                                                                                           tttttGCCATTGAGTTATTCCCGCAATCATGAATTGCGTAACCCGCCCGGCCGGACACGACAGCGTCGCATCCGGCAGGCACAGGTCGGCGATACCGCCCGATCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGt                                                       <  2:95296/150‑1 (MQ=255)
                                                                                             tttGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTc                                                     <  1:1679586/150‑1 (MQ=255)
                                                                                               tGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTg                                                   <  2:16182/150‑1 (MQ=255)
                                                                                               tGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTg                                                   <  2:1427342/150‑1 (MQ=255)
                                                                                                 ccATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTa                                                 >  2:1623271/1‑150 (MQ=255)
                                                                                                 ccATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTa                                                 <  2:1173886/150‑1 (MQ=255)
                                                                                                 ccATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGAGTCGCATCCGGCAGTCACAGGTAGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCATAACAGTTCTGTa                                                 >  1:1503747/1‑150 (MQ=255)
                                                                                                   aTTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTATACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAg                                               <  1:87901/150‑1 (MQ=255)
                                                                                                    ttGAGTTATTCCCGCCAGCATGTCTTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCTTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGTGAATACCTTTGATGCCAGAACAGTTCTGTAAGa                                              <  2:85109/150‑1 (MQ=255)
                                                                                                     tGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGAt                                             >  1:140404/1‑150 (MQ=255)
                                                                                                      gAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATACGGCAGTAACCGGTCGGCGCTACCGGCCGCTCCGTATTGTACGAAAATTTACGGGAATTACGTTGTTGCCAGTACCGGTCTGTAAGAtt                                            >  1:1090395/1‑150 (MQ=255)
                                                                                                       aGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCCTCCGGCAGTCACAGGTCGGCGATTCCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGAttt                                           >  2:131859/1‑150 (MQ=255)
                                                                                                       aGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGAttt                                           <  1:256542/150‑1 (MQ=255)
                                                                                                          tATTCCCGCCATGATGTATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCTCAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGATCAGTTCTGTAAGATTTTTa                                        <  1:1146536/150‑1 (MQ=255)
                                                                                                            ttCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGa                                      <  2:1621070/150‑1 (MQ=255)
                                                                                                                 gCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATACGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTTCGAATATTTCCGGGAATTCCGTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATc                                 >  2:586748/1‑150 (MQ=255)
                                                                                                                  ccATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCa                                <  2:1561370/150‑1 (MQ=255)
                                                                                                                     tcatGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAgcg                             >  2:737713/1‑150 (MQ=255)
                                                                                                                            ttGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcg                      <  1:1251851/150‑1 (MQ=255)
                                                                                                                              gCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggc                    >  1:996332/1‑150 (MQ=255)
                                                                                                                              gCGTAACCCGCCCTGCCGGACACGACAGCGTCGAATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTAGACGAATATTTCCGGGAATTCCTGTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcgcc                    >  2:276752/1‑148 (MQ=255)
                                                                                                                               ggTAACCCGCCCTGCCGGACTCGACAGCGTCGAATCCGTCAGTCACAGGTTGGCGATTACGCCCGCTGCGTATTCTACGAATATTTGCGGGAATTCCTTTGATGCCAGAACTGTTGTGTAAGATTTTTAGAACATCAGCGCCGTAcggcg                   <  2:1698991/149‑1 (MQ=255)
                                                                                                                               cGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGTAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggcg                   >  2:1198504/1‑150 (MQ=255)
                                                                                                                                gTAACCCGCCCTGCCGGACCCGACCGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTAGTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTATGTATGCTTTTTAGAAAATCCGCGCCGTAcggcgg                  >  2:1088015/1‑150 (MQ=255)
                                                                                                                                gTAACCCGCCCTGCCGGACCCGACAGCGGCGCATCCGGCATTCACAGGTCGGCGATAACGCCCGCTCCGTATTCTACGAATTTTACCGGGAATTCCTTTGCTTCCAGAACAGTTCTGGAATATTTTTAGAACATCAGCGCCGTAaggagg                  >  2:1082109/1‑150 (MQ=255)
                                                                                                                                 tAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                 <  1:30249/150‑1 (MQ=255)
                                                                                                                                 tAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                 >  1:504835/1‑150 (MQ=255)
                                                                                                                                 aaaCCCGCCCAGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                 <  2:235552/149‑1 (MQ=255)
                                                                                                                                  aaCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGgcggcg                <  2:1521533/150‑1 (MQ=255)
                                                                                                                                  aaCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGgcggcg                <  2:1514991/150‑1 (MQ=255)
                                                                                                                                    cccGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGtt              <  1:999247/150‑1 (MQ=255)
                                                                                                                                     ccGCCCTGCCGGACACGGCAGCGTCGCATCCGGCAGTCACCGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAAGTCCTTGGAGGCCAGCACAGTGCGGTAAGTGTTTTAGACCATCAGCGCCGTCCGGCGGCGttt             >  1:1061031/1‑150 (MQ=255)
                                                                                                                                       gCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGttttt           <  2:16891/150‑1 (MQ=255)
                                                                                                                                          cTGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg        >  1:42190/1‑150 (MQ=255)
                                                                                                                                          cTGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg        >  1:1424018/1‑150 (MQ=255)
                                                                                                                                          cTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg        <  1:1635876/150‑1 (MQ=255)
                                                                                                                                           tGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTgc       >  2:1187903/1‑150 (MQ=255)
                                                                                                                                             ccGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTgcgc     <  2:214376/150‑1 (MQ=255)
                                                                                                                                              cGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCt    >  1:675002/1‑150 (MQ=255)
                                                                                                                                               ggACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTc   <  2:416972/150‑1 (MQ=255)
                                                                                                                                                gACACGTCAGCGTCGCCTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCa  <  2:219622/150‑1 (MQ=255)
                                                                                                                                                gACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCa  <  1:1685781/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTTAATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCA  >  NC_000913/2837325‑2837618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: