Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,837,477 A→G 5.1% intergenic (‑52/+101) hypF ← / ← hydN carbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein/formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,837,4770AG 5.1% 348.7 / 6.8 119intergenic (‑52/+101)hypF/hydNcarbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein/formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
Reads supporting (aligned to +/- strand):  ref base A (52/59);  new base G (4/2);  total (58/61)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 120 total aligned reads displayed.

AATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTC  >  NC_000913/2837328‑2837625
                                                                                                                                                     |                                                                                                                                                    
 aTTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAg                                                                                                                                                     <  1:679099/150‑1 (MQ=255)
 aTTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAg                                                                                                                                                     >  1:1180888/1‑150 (MQ=255)
      tgtgCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAATATGTGTGTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCg                                                                                                                                                <  1:83720/150‑1 (MQ=255)
         gCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCAt                                                                                                                                             <  1:833510/150‑1 (MQ=255)
               ggCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGATGCGACGCTGTCGTGTCCGGCa                                                                                                                                       <  2:1388424/149‑1 (MQ=255)
                gCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAg                                                                                                                                      >  2:1251851/1‑150 (MQ=255)
                gCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAg                                                                                                                                      >  1:219622/1‑150 (MQ=255)
                gCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAg                                                                                                                                      >  1:1622004/1‑150 (MQ=255)
                  cAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTc                                                                                                                                    >  1:1583147/1‑150 (MQ=255)
                    gACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTACTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTcac                                                                                                                                  >  1:16891/1‑150 (MQ=255)
                       aaaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTGTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAgg                                                                                                                               <  1:70977/150‑1 (MQ=255)
                        aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                              >  2:420447/1‑150 (MQ=255)
                        aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                              >  2:1619984/1‑150 (MQ=255)
                        aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                              >  2:944474/1‑150 (MQ=255)
                        aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                              <  1:670135/150‑1 (MQ=255)
                           ggACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                           <  1:605474/150‑1 (MQ=255)
                           ggACGAAAACCGACGCCCTGCACTTTGCCACCAATACGCAGTGGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                           <  1:1537593/150‑1 (MQ=255)
                           ggACGAAAACCGACGACCTGAACTTTGCCACGAATACGCCGTTGGACACCGAAAGATGTGTTTTTTGCCATTGAGGTATTCCCGCCCTCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                           <  2:126446/150‑1 (MQ=255)
                            gACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACAGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCAACCGGCATTCACAGGTCgtc                                                                                                                          >  1:214376/1‑148 (MQ=255)
                             aCGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCg                                                                                                                         <  2:1128192/150‑1 (MQ=255)
                                  aaCCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCCCAGGTCGGCGATAcc                                                                                                                    >  2:30249/1‑150 (MQ=255)
                                   aCCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccg                                                                                                                   >  2:87901/1‑150 (MQ=255)
                                    ccGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgc                                                                                                                  >  1:610217/1‑150 (MQ=255)
                                    ccGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgc                                                                                                                  >  1:1427342/1‑150 (MQ=255)
                                      gACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgccc                                                                                                                >  2:1679586/1‑150 (MQ=255)
                                        cGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgcccgc                                                                                                              >  1:1705033/1‑150 (MQ=255)
                                          cccTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTc                                                                                                            >  2:666286/1‑150 (MQ=255)
                                           ccTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTcc                                                                                                           <  1:343166/150‑1 (MQ=255)
                                            cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                          <  2:1521072/150‑1 (MQ=255)
                                            cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                          <  1:1104161/150‑1 (MQ=255)
                                            cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                          <  1:1272715/150‑1 (MQ=255)
                                             tGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGt                                                                                                         <  2:638553/150‑1 (MQ=255)
                                              gCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTa                                                                                                        >  2:973578/1‑150 (MQ=255)
                                                    tGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTAc                                                                                                  >  1:977539/1‑150 (MQ=255)
                                                    tGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTAc                                                                                                  >  1:1437074/1‑150 (MQ=255)
                                                     gCCACGATTACGCATTTGGAAACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCTCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACg                                                                                                 <  2:400532/150‑1 (MQ=255)
                                                     gCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACg                                                                                                 <  1:945587/150‑1 (MQ=255)
                                                     gCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACg                                                                                                 >  1:1621070/1‑150 (MQ=255)
                                                       cACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGaa                                                                                               <  1:722138/150‑1 (MQ=255)
                                                                gCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCgg                                                                                      <  2:1148861/150‑1 (MQ=255)
                                                                 cAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCggg                                                                                     >  1:827449/1‑150 (MQ=255)
                                                                 cAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCggg                                                                                     >  1:1173886/1‑150 (MQ=255)
                                                                  aGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGa                                                                                    >  1:961251/1‑150 (MQ=255)
                                                                   gTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGaa                                                                                   >  1:1521533/1‑150 (MQ=255)
                                                                   gTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGaa                                                                                   >  1:1514991/1‑150 (MQ=255)
                                                                      ggACACCGCAAGATGGGTGTTTTGCCATTGAGTTCTTCCCGCCATCATGAATTGCGTACCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTc                                                                                <  1:345141/150‑1 (MQ=255)
                                                                         cacCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCtt                                                                             >  2:1343069/1‑150 (MQ=255)
                                                                           ccGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTg                                                                           <  1:420447/150‑1 (MQ=255)
                                                                           ccGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTg                                                                           <  1:1619984/150‑1 (MQ=255)
                                                                            cGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGa                                                                          <  1:600311/150‑1 (MQ=255)
                                                                            cGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGa                                                                          <  2:1583147/150‑1 (MQ=255)
                                                                              cAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATg                                                                        <  2:1180888/150‑1 (MQ=255)
                                                                              cAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGc                                                                       <  1:1265694/150‑1 (MQ=255)
                                                                                aGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGcc                                                                      <  1:516541/150‑1 (MQ=255)
                                                                                  aTGTGGTTTTTGCCATTCAGTTATACCCGCCATCATGAATTGCGGAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAg                                                                    <  2:1485458/150‑1 (MQ=255)
                                                                                     tgtTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAAc                                                                 >  2:351382/1‑150 (MQ=255)
                                                                                        tttttGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGt                                                              <  2:610217/150‑1 (MQ=255)
                                                                                        tttttGCCATTGAGTTATTCCCGCAATCATGAATTGCGTAACCCGCCCGGCCGGACACGACAGCGTCGCATCCGGCAGGCACAGGTCGGCGATACCGCCCGATCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGt                                                              <  2:95296/150‑1 (MQ=255)
                                                                                          tttGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTc                                                            <  1:1679586/150‑1 (MQ=255)
                                                                                            tGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTg                                                          <  2:1427342/150‑1 (MQ=255)
                                                                                              ccATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTa                                                        >  2:1623271/1‑150 (MQ=255)
                                                                                              ccATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGAGTCGCATCCGGCAGTCACAGGTAGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCATAACAGTTCTGTa                                                        >  1:1503747/1‑150 (MQ=255)
                                                                                               cATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTaa                                                       <  2:1122193/150‑1 (MQ=255)
                                                                                                aTTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTATACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAg                                                      <  1:87901/150‑1 (MQ=255)
                                                                                                 ttGAGTTATTCCCGCCAGCATGTCTTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCTTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGTGAATACCTTTGATGCCAGAACAGTTCTGTAAGa                                                     <  2:85109/150‑1 (MQ=255)
                                                                                                  tGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGAt                                                    >  1:140404/1‑150 (MQ=255)
                                                                                                   gAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATACGGCAGTAACCGGTCGGCGCTACCGGCCGCTCCGTATTGTACGAAAATTTACGGGAATTACGTTGTTGCCAGTACCGGTCTGTAAGAtt                                                   >  1:1090395/1‑150 (MQ=255)
                                                                                                    aGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGAttt                                                  <  1:256542/150‑1 (MQ=255)
                                                                                                       tATTCCCGCCATGATGTATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCTCAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGATCAGTTCTGTAAGATTTTTa                                               <  1:1146536/150‑1 (MQ=255)
                                                                                                         ttCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGa                                             <  2:1621070/150‑1 (MQ=255)
                                                                                                              gCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATACGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTTCGAATATTTCCGGGAATTCCGTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATc                                        >  2:586748/1‑150 (MQ=255)
                                                                                                               ccATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCa                                       <  2:1561370/150‑1 (MQ=255)
                                                                                                                  tcatGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAgcg                                    >  2:737713/1‑150 (MQ=255)
                                                                                                                      gAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGt                                >  1:416972/1‑150 (MQ=255)
                                                                                                                         ttGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcg                             <  1:1251851/150‑1 (MQ=255)
                                                                                                                           gCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggc                           >  1:996332/1‑150 (MQ=255)
                                                                                                                            cGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGTAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggcg                          >  2:1198504/1‑150 (MQ=255)
                                                                                                                             gTAACCCGCCCTGCCGGACCCGACCGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTAGTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTATGTATGCTTTTTAGAAAATCCGCGCCGTAcggcgg                         >  2:1088015/1‑150 (MQ=255)
                                                                                                                             gTAACCCGCCCTGCCGGACCCGACAGCGGCGCATCCGGCATTCACAGGTCGGCGATAACGCCCGCTCCGTATTCTACGAATTTTACCGGGAATTCCTTTGCTTCCAGAACAGTTCTGGAATATTTTTAGAACATCAGCGCCGTAaggagg                         >  2:1082109/1‑150 (MQ=255)
                                                                                                                              tAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                        >  1:504835/1‑150 (MQ=255)
                                                                                                                              aaaCCCGCCCAGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                        <  2:235552/149‑1 (MQ=255)
                                                                                                                               aaCCCGCCCTGCCGGCCACGACAGCGTCGCATCCGGCAGGCACCGGTCGGCGATACCGCCCGCTCCGTATTATACGACGATTTGCGGGCTTTCCTGTGATGCCAGCACAGTTCTGTAAGATTTTTTGAACATCAGCGCCGTACGgcggcg                       >  2:1567153/1‑150 (MQ=255)
                                                                                                                               aaCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGgcggcg                       <  2:1521533/150‑1 (MQ=255)
                                                                                                                                 cccGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggcgctgat                     <  1:973578/150‑6 (MQ=255)
                                                                                                                                  ccGCCCTGCCGGACACGGCAGCGTCGCATCCGGCAGTCACCGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAAGTCCTTGGAGGCCAGCACAGTGCGGTAAGTGTTTTAGACCATCAGCGCCGTCCGGCGGCGttt                    >  1:1061031/1‑150 (MQ=255)
                                                                                                                                    gCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGttttt                  <  2:16891/150‑1 (MQ=255)
                                                                                                                                       cTGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg               >  1:1424018/1‑150 (MQ=255)
                                                                                                                                       cTGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg               >  1:42190/1‑150 (MQ=255)
                                                                                                                                       cTGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg               >  1:676131/1‑150 (MQ=255)
                                                                                                                                       cTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg               <  1:1635876/150‑1 (MQ=255)
                                                                                                                                        tGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTgc              >  2:1187903/1‑150 (MQ=255)
                                                                                                                                         gCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTgcg             >  2:1435072/1‑150 (MQ=255)
                                                                                                                                          ccGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTgcgc            <  2:214376/150‑1 (MQ=255)
                                                                                                                                           cGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCt           >  1:675002/1‑150 (MQ=255)
                                                                                                                                            taacacGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGAAGCCAGAACAGTTCTGAAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCta          >  1:142091/3‑149 (MQ=255)
                                                                                                                                            ggACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTc          <  2:416972/150‑1 (MQ=255)
                                                                                                                                             gACACGTCAGCGTCGCCTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCa         <  2:219622/150‑1 (MQ=255)
                                                                                                                                                   aCAGCGTCGCATCCGGCAGTCACAGGTCGGCGATATCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAgttgtt   >  2:190837/1‑150 (MQ=255)
                                                                                                                                                   aCAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAgttgtt   <  2:1705033/150‑1 (MQ=255)
                                                                                                                                                    cAGCGTCGCATCCGGCAGTCAAAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTc  >  1:1115307/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AATTGCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTC  >  NC_000913/2837328‑2837625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: