Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,837,479 C→T 5.1% intergenic (‑54/+99) hypF ← / ← hydN carbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein/formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,837,4790CT 5.1% 381.6 / 4.0 117intergenic (‑54/+99)hypF/hydNcarbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein/formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
Reads supporting (aligned to +/- strand):  ref base C (52/58);  new base T (4/2);  total (57/60)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01

Only 100 of 119 total aligned reads displayed.

GCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAG  >  NC_000913/2837332‑2837628
                                                                                                                                                   |                                                                                                                                                     
gCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGt                                                                                                                                                     >  1:470924/1‑150 (MQ=255)
  tgtgCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAATATGTGTGTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCg                                                                                                                                                   <  1:83720/150‑1 (MQ=255)
     gCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCAt                                                                                                                                                <  1:833510/150‑1 (MQ=255)
           ggCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGATGCGACGCTGTCGTGTCCGGCa                                                                                                                                          <  2:1388424/149‑1 (MQ=255)
            gCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAg                                                                                                                                         >  1:1622004/1‑150 (MQ=255)
            gCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAg                                                                                                                                         >  2:1251851/1‑150 (MQ=255)
              cAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTc                                                                                                                                       >  1:1583147/1‑150 (MQ=255)
                gACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTACTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTcac                                                                                                                                     >  1:16891/1‑150 (MQ=255)
                 aCAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGATGCGACGCTGTCGTGTCCGGCAgggcgg                                                                                                                                    <  1:145781/150‑6 (MQ=38)
                   aaaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTGTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAgg                                                                                                                                  <  1:70977/150‑1 (MQ=255)
                    aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                                 >  2:944474/1‑150 (MQ=255)
                    aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                                 >  2:420447/1‑150 (MQ=255)
                    aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                                 <  1:670135/150‑1 (MQ=255)
                       ggACGAAAACCGACGCCCTGCACTTTGCCACCAATACGCAGTGGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                              <  1:1537593/150‑1 (MQ=255)
                       ggACGAAAACCGACGACCTGAACTTTGCCACGAATACGCCGTTGGACACCGAAAGATGTGTTTTTTGCCATTGAGGTATTCCCGCCCTCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                              <  2:126446/150‑1 (MQ=255)
                        gACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACAGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCAACCGGCATTCACAGGTCgtc                                                                                                                             >  1:214376/1‑148 (MQ=255)
                         aCGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCg                                                                                                                            <  2:1128192/150‑1 (MQ=255)
                            aaaaCCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGCCACGCCCGCGTCGCATCCGGCAGGCACAGGTCGGCGAtc                                                                                                                         >  1:1587314/1‑149 (MQ=255)
                               aCCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccg                                                                                                                      >  2:87901/1‑150 (MQ=255)
                                ccGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgc                                                                                                                     >  1:1427342/1‑150 (MQ=255)
                                ccGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgc                                                                                                                     >  1:610217/1‑150 (MQ=255)
                                  gACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgccc                                                                                                                   >  2:1679586/1‑150 (MQ=255)
                                    cGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgcccgc                                                                                                                 >  1:1705033/1‑150 (MQ=255)
                                      cccTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTc                                                                                                               >  2:666286/1‑150 (MQ=255)
                                       ccTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTcc                                                                                                              <  1:343166/150‑1 (MQ=255)
                                        cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                             <  1:1272715/150‑1 (MQ=255)
                                        cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                             <  1:1104161/150‑1 (MQ=255)
                                         tGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGt                                                                                                            <  2:638553/150‑1 (MQ=255)
                                          gCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTa                                                                                                           >  2:973578/1‑150 (MQ=255)
                                                tGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTAc                                                                                                     >  1:977539/1‑150 (MQ=255)
                                                tGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTAc                                                                                                     <  2:745320/150‑1 (MQ=255)
                                                tGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTAc                                                                                                     >  1:1437074/1‑150 (MQ=255)
                                                 gCCACGATTACGCATTTGGAAACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCTCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACg                                                                                                    <  2:400532/150‑1 (MQ=255)
                                                 gCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACg                                                                                                    <  1:945587/150‑1 (MQ=255)
                                                 gCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACg                                                                                                    >  1:1621070/1‑150 (MQ=255)
                                                   cACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGaa                                                                                                  <  1:722138/150‑1 (MQ=255)
                                                     cGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAata                                                                                                >  2:516541/1‑150 (MQ=255)
                                                            gCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCgg                                                                                         <  2:1148861/150‑1 (MQ=255)
                                                             cAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCggg                                                                                        >  1:1173886/1‑150 (MQ=255)
                                                             cAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCggg                                                                                        >  1:827449/1‑150 (MQ=255)
                                                             cAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCggg                                                                                        <  1:648512/150‑1 (MQ=255)
                                                              aGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGa                                                                                       >  1:961251/1‑150 (MQ=255)
                                                               gTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGaa                                                                                      >  1:1514991/1‑150 (MQ=255)
                                                                  ggACACCGCAAGATGGGTGTTTTGCCATTGAGTTCTTCCCGCCATCATGAATTGCGTACCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTc                                                                                   <  1:345141/150‑1 (MQ=255)
                                                                       ccGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTg                                                                              <  1:1619984/150‑1 (MQ=255)
                                                                        cGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGa                                                                             <  1:600311/150‑1 (MQ=255)
                                                                        cGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGa                                                                             <  2:1583147/150‑1 (MQ=255)
                                                                          cAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATg                                                                           <  2:1180888/150‑1 (MQ=255)
                                                                          cAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGc                                                                          <  1:1265694/150‑1 (MQ=255)
                                                                            aGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGcc                                                                         <  1:516541/150‑1 (MQ=255)
                                                                            aGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGACGGACACGACAGCGTCGCATCCGGCAGGCCCAGGTAGGCGATACCGCCCGCTCCGTATTCTGAGGATATTTACGGGAATTCCTTTGATGcc                                                                         >  1:1286052/1‑150 (MQ=255)
                                                                              aTGTGGTTTTTGCCATTCAGTTATACCCGCCATCATGAATTGCGGAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAg                                                                       <  2:1485458/150‑1 (MQ=255)
                                                                                 tgtTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAAc                                                                    >  2:351382/1‑150 (MQ=255)
                                                                                    tttttGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGt                                                                 <  2:610217/150‑1 (MQ=255)
                                                                                    tttttGCCATTGAGTTATTCCCGCAATCATGAATTGCGTAACCCGCCCGGCCGGACACGACAGCGTCGCATCCGGCAGGCACAGGTCGGCGATACCGCCCGATCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGt                                                                 <  2:95296/150‑1 (MQ=255)
                                                                                      tttGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTc                                                               <  1:1679586/150‑1 (MQ=255)
                                                                                        tGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTg                                                             <  2:1427342/150‑1 (MQ=255)
                                                                                        tGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTg                                                             <  2:16182/150‑1 (MQ=255)
                                                                                          ccATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTa                                                           >  2:1623271/1‑150 (MQ=255)
                                                                                          ccATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGAGTCGCATCCGGCAGTCACAGGTAGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCATAACAGTTCTGTa                                                           >  1:1503747/1‑150 (MQ=255)
                                                                                           cATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTaa                                                          <  2:1122193/150‑1 (MQ=255)
                                                                                            aTTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTATACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAg                                                         <  1:87901/150‑1 (MQ=255)
                                                                                             ttGAGTTATTCCCGCCAGCATGTCTTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCTTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGTGAATACCTTTGATGCCAGAACAGTTCTGTAAGa                                                        <  2:85109/150‑1 (MQ=255)
                                                                                              tGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGAt                                                       >  1:140404/1‑150 (MQ=255)
                                                                                               gAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATACGGCAGTAACCGGTCGGCGCTACCGGCCGCTCCGTATTGTACGAAAATTTACGGGAATTACGTTGTTGCCAGTACCGGTCTGTAAGAtt                                                      >  1:1090395/1‑150 (MQ=255)
                                                                                                aGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCCTCCGGCAGTCACAGGTCGGCGATTCCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGAttt                                                     >  2:131859/1‑150 (MQ=255)
                                                                                                aGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGAttt                                                     <  1:256542/150‑1 (MQ=255)
                                                                                                   tATTCCCGCCATGATGTATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCTCAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGATCAGTTCTGTAAGATTTTTa                                                  <  1:1146536/150‑1 (MQ=255)
                                                                                                     ttCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGa                                                <  2:1621070/150‑1 (MQ=255)
                                                                                                           ccATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCa                                          <  2:1561370/150‑1 (MQ=255)
                                                                                                              tcatGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAgcg                                       >  2:737713/1‑150 (MQ=255)
                                                                                                                  gAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGt                                   >  1:416972/1‑150 (MQ=255)
                                                                                                                       gCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggc                              >  1:996332/1‑150 (MQ=255)
                                                                                                                       gCGTAACCCGCCCTGCCGGACACGACAGCGTCGAATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTAGACGAATATTTCCGGGAATTCCTGTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcgcc                              >  2:276752/1‑148 (MQ=255)
                                                                                                                        ggTAACCCGCCCTGCCGGACTCGACAGCGTCGAATCCGTCAGTCACAGGTTGGCGATTACGCCCGCTGCGTATTCTACGAATATTTGCGGGAATTCCTTTGATGCCAGAACTGTTGTGTAAGATTTTTAGAACATCAGCGCCGTAcggcg                             <  2:1698991/149‑1 (MQ=255)
                                                                                                                        cGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGTAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggcg                             >  2:1198504/1‑150 (MQ=255)
                                                                                                                         gTAACCCGCCCTGCCGGACCCGACCGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTAGTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTATGTATGCTTTTTAGAAAATCCGCGCCGTAcggcgg                            >  2:1088015/1‑150 (MQ=255)
                                                                                                                         gTAACCCGCCCTGCCGGACCCGACAGCGGCGCATCCGGCATTCACAGGTCGGCGATAACGCCCGCTCCGTATTCTACGAATTTTACCGGGAATTCCTTTGCTTCCAGAACAGTTCTGGAATATTTTTAGAACATCAGCGCCGTAaggagg                            >  2:1082109/1‑150 (MQ=255)
                                                                                                                          tAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                           <  1:30249/150‑1 (MQ=255)
                                                                                                                          tAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                           >  1:504835/1‑150 (MQ=255)
                                                                                                                          aaaCCCGCCCAGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                           <  2:235552/149‑1 (MQ=255)
                                                                                                                           aaCCCGCCCTGCCGGCCACGACAGCGTCGCATCCGGCAGGCACCGGTCGGCGATACCGCCCGCTCCGTATTATACGACGATTTGCGGGCTTTCCTGTGATGCCAGCACAGTTCTGTAAGATTTTTTGAACATCAGCGCCGTACGgcggcg                          >  2:1567153/1‑150 (MQ=255)
                                                                                                                           aaCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGgcggcg                          <  2:1521533/150‑1 (MQ=255)
                                                                                                                           aaCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGgcggcg                          <  2:1514991/150‑1 (MQ=255)
                                                                                                                             cccGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggcgctgat                        <  1:973578/150‑6 (MQ=255)
                                                                                                                              ccGCCCTGCCGGACACGGCAGCGTCGCATCCGGCAGTCACCGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAAGTCCTTGGAGGCCAGCACAGTGCGGTAAGTGTTTTAGACCATCAGCGCCGTCCGGCGGCGttt                       >  1:1061031/1‑150 (MQ=255)
                                                                                                                                gCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGttttt                     <  2:16891/150‑1 (MQ=255)
                                                                                                                                   cTGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg                  >  1:676131/1‑150 (MQ=255)
                                                                                                                                   cTGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg                  >  1:42190/1‑150 (MQ=255)
                                                                                                                                    tGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTgc                 >  2:1187903/1‑150 (MQ=255)
                                                                                                                                     gCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTgcg                >  2:1435072/1‑150 (MQ=255)
                                                                                                                                      ccGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTgcgc               <  2:214376/150‑1 (MQ=255)
                                                                                                                                       cGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCt              >  1:675002/1‑150 (MQ=255)
                                                                                                                                        taacacGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGAAGCCAGAACAGTTCTGAAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCta             >  1:142091/3‑149 (MQ=255)
                                                                                                                                        ggACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTc             <  2:416972/150‑1 (MQ=255)
                                                                                                                                         gACACGTCAGCGTCGCCTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCa            <  2:219622/150‑1 (MQ=255)
                                                                                                                                         gACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCa            <  1:1685781/150‑1 (MQ=255)
                                                                                                                                               aCAGCGTCGCATCCGGCAGTCACAGGTCGGCGATATCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAgttgtt      >  2:190837/1‑150 (MQ=255)
                                                                                                                                                cAGCGTCGCATCCGGCAGTCAAAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTc     >  1:1115307/1‑150 (MQ=255)
                                                                                                                                                   cGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAg  >  2:188516/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCTGTGCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAG  >  NC_000913/2837332‑2837628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: