Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,837,485 A→G 5.0% intergenic (‑60/+93) hypF ← / ← hydN carbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein/formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,837,4850AG 5.0% 342.6 / 6.3 121intergenic (‑60/+93)hypF/hydNcarbamoyl phosphate phosphatase and [NiFe] hydrogenase maturation protein/formate dehydrogenase‑H, [4Fe‑4S] ferredoxin subunit
Reads supporting (aligned to +/- strand):  ref base A (55/57);  new base G (4/2);  total (60/61)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01

Only 100 of 121 total aligned reads displayed.

GCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTAT  >  NC_000913/2837337‑2837633
                                                                                                                                                    |                                                                                                                                                    
gCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCAt                                                                                                                                                     <  1:833510/150‑1 (MQ=255)
      ggCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGATGCGACGCTGTCGTGTCCGGCa                                                                                                                                               <  2:1388424/149‑1 (MQ=255)
       gCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAg                                                                                                                                              >  1:219622/1‑150 (MQ=255)
       gCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAg                                                                                                                                              >  2:1251851/1‑150 (MQ=255)
       gCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAg                                                                                                                                              >  1:1622004/1‑150 (MQ=255)
         cAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTc                                                                                                                                            >  1:1583147/1‑150 (MQ=255)
           gACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTACTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTcac                                                                                                                                          >  1:16891/1‑150 (MQ=255)
            aCAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGATGCGACGCTGTCGTGTCCGGCAgggcgg                                                                                                                                         <  1:145781/150‑6 (MQ=38)
              aaaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTGTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAgg                                                                                                                                       <  1:70977/150‑1 (MQ=255)
               aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                                      <  1:670135/150‑1 (MQ=255)
               aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                                      >  2:944474/1‑150 (MQ=255)
               aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                                      >  2:420447/1‑150 (MQ=255)
               aaCGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGt                                                                                                                                      >  2:1619984/1‑150 (MQ=255)
                  ggACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                                   <  1:605474/150‑1 (MQ=255)
                  ggACGAAAACCGACGCCCTGCACTTTGCCACCAATACGCAGTGGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                                   <  1:1537593/150‑1 (MQ=255)
                  ggACGAAAACCGACGACCTGAACTTTGCCACGAATACGCCGTTGGACACCGAAAGATGTGTTTTTTGCCATTGAGGTATTCCCGCCCTCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCgg                                                                                                                                   <  2:126446/150‑1 (MQ=255)
                   gACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGc                                                                                                                                  <  1:891451/150‑1 (MQ=255)
                   gACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACAGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCAACCGGCATTCACAGGTCgtc                                                                                                                                  >  1:214376/1‑148 (MQ=255)
                    aCGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCg                                                                                                                                 <  2:1128192/150‑1 (MQ=255)
                       aaaaCCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGCCACGCCCGCGTCGCATCCGGCAGGCACAGGTCGGCGAtc                                                                                                                              >  1:1587314/1‑149 (MQ=255)
                         aaCCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCCCAGGTCGGCGATAcc                                                                                                                            >  2:30249/1‑150 (MQ=255)
                          aCCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccg                                                                                                                           >  2:87901/1‑150 (MQ=255)
                           ccGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgc                                                                                                                          >  1:1427342/1‑150 (MQ=255)
                           ccGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgc                                                                                                                          >  1:610217/1‑150 (MQ=255)
                             gACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgccc                                                                                                                        >  2:1679586/1‑150 (MQ=255)
                               cGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATAccgcccgc                                                                                                                      >  1:1705033/1‑150 (MQ=255)
                                 cccTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTc                                                                                                                    >  2:666286/1‑150 (MQ=255)
                                  ccTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTcc                                                                                                                   <  1:343166/150‑1 (MQ=255)
                                   cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                                  <  2:1521072/150‑1 (MQ=255)
                                   cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                                  <  1:1104161/150‑1 (MQ=255)
                                   cTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCg                                                                                                                  <  1:1272715/150‑1 (MQ=255)
                                     gCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTa                                                                                                                >  2:973578/1‑150 (MQ=255)
                                           tGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTAc                                                                                                          <  2:745320/150‑1 (MQ=255)
                                           tGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTAc                                                                                                          >  1:977539/1‑150 (MQ=255)
                                           tGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTAc                                                                                                          >  1:1437074/1‑150 (MQ=255)
                                              cACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGaa                                                                                                       <  1:722138/150‑1 (MQ=255)
                                                cGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAata                                                                                                     >  2:516541/1‑150 (MQ=255)
                                                       gCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCgg                                                                                              <  2:1148861/150‑1 (MQ=255)
                                                        cAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCggg                                                                                             >  1:827449/1‑150 (MQ=255)
                                                         aGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGa                                                                                            >  1:961251/1‑150 (MQ=255)
                                                          gTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGaa                                                                                           >  1:1521533/1‑150 (MQ=255)
                                                             ggACACCGCAAGATGGGTGTTTTGCCATTGAGTTCTTCCCGCCATCATGAATTGCGTACCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTc                                                                                        <  1:345141/150‑1 (MQ=255)
                                                                cacCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCtt                                                                                     >  2:1343069/1‑150 (MQ=255)
                                                                  ccGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTg                                                                                   <  1:1619984/150‑1 (MQ=255)
                                                                  ccGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTg                                                                                   <  1:420447/150‑1 (MQ=255)
                                                                   cGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGa                                                                                  <  1:600311/150‑1 (MQ=255)
                                                                     cAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGc                                                                               <  1:1265694/150‑1 (MQ=255)
                                                                       aGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGcc                                                                              <  1:516541/150‑1 (MQ=255)
                                                                       aGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGACGGACACGACAGCGTCGCATCCGGCAGGCCCAGGTAGGCGATACCGCCCGCTCCGTATTCTGAGGATATTTACGGGAATTCCTTTGATGcc                                                                              >  1:1286052/1‑150 (MQ=255)
                                                                            tgtTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAAc                                                                         >  2:351382/1‑150 (MQ=255)
                                                                               tttttGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGt                                                                      <  2:610217/150‑1 (MQ=255)
                                                                               tttttGCCATTGAGTTATTCCCGCAATCATGAATTGCGTAACCCGCCCGGCCGGACACGACAGCGTCGCATCCGGCAGGCACAGGTCGGCGATACCGCCCGATCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGt                                                                      <  2:95296/150‑1 (MQ=255)
                                                                                 tttGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTc                                                                    <  1:1679586/150‑1 (MQ=255)
                                                                                   tGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTg                                                                  <  2:16182/150‑1 (MQ=255)
                                                                                   tGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTg                                                                  <  2:1427342/150‑1 (MQ=255)
                                                                                     ccATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTa                                                                <  2:1173886/150‑1 (MQ=255)
                                                                                     ccATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTa                                                                >  2:1623271/1‑150 (MQ=255)
                                                                                     ccATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGAGTCGCATCCGGCAGTCACAGGTAGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCATAACAGTTCTGTa                                                                >  1:1503747/1‑150 (MQ=255)
                                                                                      cATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTaa                                                               <  2:1122193/150‑1 (MQ=255)
                                                                                       aTTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTATACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAg                                                              <  1:87901/150‑1 (MQ=255)
                                                                                         tGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGAt                                                            >  1:140404/1‑150 (MQ=255)
                                                                                          gAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATACGGCAGTAACCGGTCGGCGCTACCGGCCGCTCCGTATTGTACGAAAATTTACGGGAATTACGTTGTTGCCAGTACCGGTCTGTAAGAtt                                                           >  1:1090395/1‑150 (MQ=255)
                                                                                           aGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCCTCCGGCAGTCACAGGTCGGCGATTCCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGAttt                                                          >  2:131859/1‑150 (MQ=255)
                                                                                              tATTCCCGCCATGATGTATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCTCAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGATCAGTTCTGTAAGATTTTTa                                                       <  1:1146536/150‑1 (MQ=255)
                                                                                                ttCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGa                                                     <  2:1621070/150‑1 (MQ=255)
                                                                                                     gCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATACGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTTCGAATATTTCCGGGAATTCCGTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATc                                                >  2:586748/1‑150 (MQ=255)
                                                                                                      ccATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCa                                               <  2:1561370/150‑1 (MQ=255)
                                                                                                         tcatGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAgcg                                            >  2:737713/1‑150 (MQ=255)
                                                                                                             gAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGt                                        >  1:416972/1‑150 (MQ=255)
                                                                                                                ttGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcg                                     <  1:1251851/150‑1 (MQ=255)
                                                                                                                  gCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggc                                   >  1:996332/1‑150 (MQ=255)
                                                                                                                  gCGTAACCCGCCCTGCCGGACACGACAGCGTCGAATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTAGACGAATATTTCCGGGAATTCCTGTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcgcc                                   >  2:276752/1‑148 (MQ=255)
                                                                                                                   ggTAACCCGCCCTGCCGGACTCGACAGCGTCGAATCCGTCAGTCACAGGTTGGCGATTACGCCCGCTGCGTATTCTACGAATATTTGCGGGAATTCCTTTGATGCCAGAACTGTTGTGTAAGATTTTTAGAACATCAGCGCCGTAcggcg                                  <  2:1698991/149‑1 (MQ=255)
                                                                                                                   cGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGTAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggcg                                  >  2:1198504/1‑150 (MQ=255)
                                                                                                                    gTAACCCGCCCTGCCGGACCCGACCGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTAGTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTATGTATGCTTTTTAGAAAATCCGCGCCGTAcggcgg                                 >  2:1088015/1‑150 (MQ=255)
                                                                                                                     tAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                                <  1:30249/150‑1 (MQ=255)
                                                                                                                     tAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                                >  1:504835/1‑150 (MQ=255)
                                                                                                                     aaaCCCGCCCAGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACggcggc                                <  2:235552/149‑1 (MQ=255)
                                                                                                                      aaCCCGCCCTGCCGGCCACGACAGCGTCGCATCCGGCAGGCACCGGTCGGCGATACCGCCCGCTCCGTATTATACGACGATTTGCGGGCTTTCCTGTGATGCCAGCACAGTTCTGTAAGATTTTTTGAACATCAGCGCCGTACGgcggcg                               >  2:1567153/1‑150 (MQ=255)
                                                                                                                      aaCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGgcggcg                               <  2:1521533/150‑1 (MQ=255)
                                                                                                                      aaCCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGgcggcg                               <  2:1514991/150‑1 (MQ=255)
                                                                                                                        cccGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTAcggcgctgat                             <  1:973578/150‑6 (MQ=255)
                                                                                                                        cccGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGtt                             <  1:999247/150‑1 (MQ=255)
                                                                                                                         ccGCCCTGCCGGACACGGCAGCGTCGCATCCGGCAGTCACCGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAAGTCCTTGGAGGCCAGCACAGTGCGGTAAGTGTTTTAGACCATCAGCGCCGTCCGGCGGCGttt                            >  1:1061031/1‑150 (MQ=255)
                                                                                                                              cTGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg                       >  1:1424018/1‑150 (MQ=255)
                                                                                                                              cTGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg                       >  1:676131/1‑150 (MQ=255)
                                                                                                                              cTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTg                       <  1:1635876/150‑1 (MQ=255)
                                                                                                                               tGCCGGATGCGACGCTGTCGTGTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTgc                      >  2:1187903/1‑150 (MQ=255)
                                                                                                                                gCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTgcg                     >  2:1435072/1‑150 (MQ=255)
                                                                                                                                  cGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCt                   >  1:675002/1‑150 (MQ=255)
                                                                                                                                   ggACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTc                  <  2:416972/150‑1 (MQ=255)
                                                                                                                                    gACACGTCAGCGTCGCCTCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCa                 <  2:219622/150‑1 (MQ=255)
                                                                                                                                          aCAGCGTCGCATCCGGCAGTCACAGGTCGGCGATATCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAgttgtt           >  2:190837/1‑150 (MQ=255)
                                                                                                                                          aCAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAgttgtt           <  2:1705033/150‑1 (MQ=255)
                                                                                                                                           cAGCGTCGCATCCGGCAGTCAAAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTc          >  1:1115307/1‑150 (MQ=255)
                                                                                                                                              cGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAg       >  2:188516/1‑150 (MQ=255)
                                                                                                                                              cGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAg       <  2:827449/150‑1 (MQ=255)
                                                                                                                                               gTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGt      >  1:253800/1‑150 (MQ=255)
                                                                                                                                                   cATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTttat  >  1:1659500/1‑150 (MQ=255)
                                                                                                                                                   cATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTATACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTATAACATCAGCGCCGTACGGCGGCGGTTTTGATCGCAGAGTTGTTAAAGTttaa  >  2:775306/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCAGCTGCCAGACAAACGGACGAAAACCGACGCCCTGCACTTTGCCACGAATACGCAGTTGGACACCGCAAGATGTGTTTTTTGCCATTGAGTTATTCCCGCCATCATGAATTGCGTAACCCGCCCTGCCGGACACGACAGCGTCGCATCCGGCAGTCACAGGTCGGCGATACCGCCCGCTCCGTATTCTACGAATATTTCCGGGAATTCCTTTGATGCCAGAACAGTTCTGTAAGATTTTTAGAACATCAGCGCCGTACGGCGGCGTTTTTCTGCGCTCAGTTGTTCAAGTTTAT  >  NC_000913/2837337‑2837633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: