Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,839,324 T→A 5.0% intergenic (‑60/‑200) ascG ← / → ascF asc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,839,3240TA 5.0% 385.9 / 6.1 130intergenic (‑60/‑200)ascG/ascFasc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand):  ref base T (58/64);  new base A (5/2);  total (64/66)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01

Only 100 of 133 total aligned reads displayed.

CTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCAC  >  NC_000913/2839175‑2839473
                                                                                                                                                     |                                                                                                                                                     
 tGGCTGACGTAGCCATTAGCTGAAAGAACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTGCAGCTTCGTCGTCATCATTTTCATCCCTTTACACGCAATGAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTg                                                                                                                                                      <  2:354929/150‑1 (MQ=255)
 tGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTg                                                                                                                                                      >  2:271936/1‑150 (MQ=255)
  ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGc                                                                                                                                                     >  1:24581/1‑150 (MQ=255)
  ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGc                                                                                                                                                     >  1:609607/1‑150 (MQ=255)
  ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGc                                                                                                                                                     >  2:1009728/1‑150 (MQ=255)
   gCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCCTCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGca                                                                                                                                                    <  2:891241/150‑1 (MQ=255)
         gTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcg                                                                                                                                              <  1:1043037/150‑1 (MQ=255)
          tAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcgg                                                                                                                                             <  1:194258/150‑1 (MQ=255)
           aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                            <  1:1608294/150‑1 (MQ=255)
           aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                            <  2:893247/150‑1 (MQ=255)
           aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggc                                                                                                                                            >  2:33830/1‑150 (MQ=255)
            gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTACAGCATCGTCGTCATCATTTTAATACCTGTACACGCAATCAACGCACTGTACTGCACCCTTTGCCGATTGTACATGCACAATctgcg                                                                                                                                           >  1:798019/1‑150 (MQ=255)
             ccATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcgg                                                                                                                                          <  1:646501/150‑1 (MQ=255)
              cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                         >  2:201763/1‑150 (MQ=255)
              cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                         <  1:460185/150‑1 (MQ=255)
                  aCCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATAATTTTGATCCCTTTACACGCAATCAACGCAGTGTACTTCACCGTTTGCCGATTTGACTAGCACAATCGGCGGGaaaa                                                                                                                                     >  2:1585415/1‑150 (MQ=255)
                     tGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAata                                                                                                                                  >  1:830650/1‑150 (MQ=255)
                     tGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAata                                                                                                                                  <  1:1403939/150‑1 (MQ=255)
                      gAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATGCCTTTACAAGCAATCAACGCAGTGTACTGCACCGTTTGCCGAATGTCCATGCACAATCGGCGGGTAAAatat                                                                                                                                 >  2:1094803/1‑150 (MQ=255)
                      gAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAatat                                                                                                                                 <  1:634430/150‑1 (MQ=255)
                             cgcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCCATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTg                                                                                                                          >  1:434542/1‑150 (MQ=255)
                              gcgGGAAACGGTCGCTTTTGAAACCCAGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGa                                                                                                                         <  1:271936/150‑1 (MQ=255)
                               cgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTACAGCATCGTCGGGATGATTTTGATCCCGTTACACGCAATCAACGCAGTGTACTGAACCGTTTTCCGAATTTACTTGCACAATCGGCGGGAAAAATATTAAGGttgc                                                                                                                        >  2:1707530/1‑147 (MQ=255)
                                 ggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTAATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTTACCg                                                                                                                      >  2:1472832/1‑150 (MQ=255)
                                     aCGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTAATACCTTTACAAGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGgtt                                                                                                                  >  1:1625881/1‑150 (MQ=255)
                                      cGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTc                                                                                                                 <  1:201763/150‑1 (MQ=255)
                                      cGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTc                                                                                                                 <  2:1480607/150‑1 (MQ=255)
                                        gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                               >  1:1000765/1‑150 (MQ=255)
                                            cTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                           <  2:1003021/150‑1 (MQ=255)
                                            cTTTTGAAACCCCGGCGCGCGTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                           >  2:45864/1‑150 (MQ=255)
                                              tttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAatat                                                                                                         >  2:134352/1‑150 (MQ=255)
                                                tGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaa                                                                                                       >  2:23384/1‑150 (MQ=255)
                                                 gAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaa                                                                                                      <  2:302675/150‑1 (MQ=255)
                                                 gAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaa                                                                                                      <  2:603693/150‑1 (MQ=255)
                                                      cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg                                                                                                 <  1:33830/150‑1 (MQ=255)
                                                        cGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGaa                                                                                               <  1:1378893/150‑1 (MQ=255)
                                                           cgcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACaa                                                                                            >  1:67977/1‑150 (MQ=255)
                                                             tgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAtt                                                                                          <  2:1268384/149‑1 (MQ=255)
                                                             cgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAtt                                                                                          >  1:216108/1‑150 (MQ=255)
                                                              gcTTCGCCACTTCCAGCATCTTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTTCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTc                                                                                         <  2:1590003/150‑1 (MQ=255)
                                                              gcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTc                                                                                         <  1:23384/150‑1 (MQ=255)
                                                                  cGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTTTACTTCACCGTTTTCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActc                                                                                     <  1:265994/150‑1 (MQ=255)
                                                                  cGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActc                                                                                     >  2:122936/1‑150 (MQ=255)
                                                                   gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                    >  2:1710612/1‑150 (MQ=255)
                                                                   gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                    >  2:210587/1‑150 (MQ=255)
                                                                    ccACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActctc                                                                                   >  2:1394242/1‑150 (MQ=255)
                                                                         tCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCt                                                                              <  2:375838/150‑1 (MQ=255)
                                                                           cAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTa                                                                            <  1:210587/150‑1 (MQ=255)
                                                                                 cgtcgtCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGt                                                                      <  2:830650/150‑1 (MQ=255)
                                                                                  gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTg                                                                     <  2:431241/150‑1 (MQ=255)
                                                                                  gtcgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTg                                                                     <  2:1584636/150‑1 (MQ=255)
                                                                                      tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTATCTTGATTATTTAGTGACaa                                                                 >  1:1009449/1‑150 (MQ=255)
                                                                                      tcatcaTTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAATCTCTTGCTTATTTAGTGACaa                                                                 >  1:1438000/1‑150 (MQ=255)
                                                                                       catcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                                >  1:557341/1‑150 (MQ=255)
                                                                                       catcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                                <  2:499236/150‑1 (MQ=255)
                                                                                       catcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTCCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                                <  2:727924/150‑1 (MQ=255)
                                                                                         tcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTa                                                              >  1:964834/1‑150 (MQ=255)
                                                                                            ttttCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTc                                                           <  2:24581/150‑1 (MQ=255)
                                                                                            ttttCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTc                                                           <  2:609607/150‑1 (MQ=255)
                                                                                             tttCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCa                                                          <  1:1358956/150‑1 (MQ=255)
                                                                                                  tCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTAtt                                                            <  2:1291815/143‑1 (MQ=255)
                                                                                                  tCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTAtt                                                            >  1:1291815/1‑143 (MQ=255)
                                                                                                  tCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAtt                                                     >  2:1332339/1‑150 (MQ=255)
                                                                                                  tCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAtt                                                     >  2:845678/1‑150 (MQ=255)
                                                                                                    ccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAtttt                                                   >  1:879732/1‑150 (MQ=255)
                                                                                                     cTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtg                                                  <  1:45864/150‑1 (MQ=255)
                                                                                                     cTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtg                                                  <  1:1710612/150‑1 (MQ=255)
                                                                                                      tttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGTGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgt                                                 <  2:772567/150‑1 (MQ=255)
                                                                                                       ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                                <  2:216108/150‑1 (MQ=255)
                                                                                                       ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                                <  1:122936/150‑1 (MQ=255)
                                                                                                       ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                                <  2:253369/150‑1 (MQ=255)
                                                                                                        tACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGa                                               >  1:169568/1‑150 (MQ=255)
                                                                                                          cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaaa                                             >  2:657214/1‑150 (MQ=255)
                                                                                                          cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaaa                                             >  1:341492/1‑150 (MQ=255)
                                                                                                          cacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaaa                                             >  1:1299674/1‑150 (MQ=255)
                                                                                                            cGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                           <  2:557341/150‑1 (MQ=255)
                                                                                                            cGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                           >  1:253782/1‑150 (MQ=255)
                                                                                                                 tCAACGCAGTTTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGACTTtg                                                  <  1:1149569/138‑1 (MQ=255)
                                                                                                                  cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGttta                                     >  2:466864/1‑149 (MQ=255)
                                                                                                                   aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                    >  1:584847/1‑150 (MQ=255)
                                                                                                                    aCGCAGTGTACTGCACCTTTTGCCGATTGTCCTTGCAAAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCtt                                   <  1:1385873/150‑1 (MQ=255)
                                                                                                                            tACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTACGt                           >  2:1194026/1‑150 (MQ=255)
                                                                                                                             aCTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGtt                          >  2:742363/1‑150 (MQ=255)
                                                                                                                             aCTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGtt                          <  2:885420/150‑1 (MQ=255)
                                                                                                                              cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGttt                         >  1:689671/1‑150 (MQ=255)
                                                                                                                              cTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTAACTCTCTTGCTAATTTAGAGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGttt                         >  2:1350362/1‑150 (MQ=255)
                                                                                                                                gCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCa                       >  1:526915/1‑150 (MQ=255)
                                                                                                                                      tttGCCGTTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAACGGTTTCACAAATATAAATAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCg                 <  2:1103145/150‑1 (MQ=255)
                                                                                                                                       ccgCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCgg                >  2:1684868/3‑150 (MQ=255)
                                                                                                                                       ccgCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGAATCgg                >  2:1311761/3‑150 (MQ=255)
                                                                                                                                        tGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGGCCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGc               <  1:1585415/150‑1 (MQ=255)
                                                                                                                                        cgCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGc               >  1:1663493/2‑150 (MQ=255)
                                                                                                                                          ccGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAACATCGGCAt             >  1:343292/1‑150 (MQ=255)
                                                                                                                                           cGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAtt            >  1:1362433/1‑150 (MQ=255)
                                                                                                                                               tGTCCTTGCAAAATGGGCGGTAAAAATTTTCTGGTGACCGGTTTCAAAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTc        <  1:931660/150‑1 (MQ=255)
                                                                                                                                                 tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg      <  2:1198228/150‑1 (MQ=255)
                                                                                                                                                 tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg      <  2:201435/150‑1 (MQ=255)
                                                                                                                                                  ccTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGt     <  2:879732/150‑1 (MQ=255)
                                                                                                                                                   cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCCCTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTGTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTc    >  2:65996/1‑150 (MQ=255)
                                                                                                                                                     tGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCAc  >  1:700506/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCAC  >  NC_000913/2839175‑2839473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: