Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,884,578 C→A 6.5% R881I (AGA→ATA) ‡ ygcB ← Cascade complex anti‑viral R‑loop helicase‑annealase Cas3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,884,5780CA 6.5% 572.5 / 19.6 181R881I (AGA→ATA) ‡ygcBCascade complex anti‑viral R‑loop helicase‑annealase Cas3
Reads supporting (aligned to +/- strand):  ref base C (80/89);  new base A (3/9);  total (83/98)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

Only 100 of 185 total aligned reads displayed.

TGAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTG  >  NC_000913/2884432‑2884727
                                                                                                                                                  |                                                                                                                                                      
tgAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTgg                                                                                                                                                     <  2:358112/150‑1 (MQ=255)
 taaTATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGt                                                                                                                                                    <  2:678618/149‑1 (MQ=255)
  aaTATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTc                                                                                                                                                   <  2:812680/150‑1 (MQ=255)
  aaTATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTc                                                                                                                                                   <  2:119862/150‑1 (MQ=255)
     ataAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTTCTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATc                                                                                                                                                <  1:391005/150‑1 (MQ=255)
     ataAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATc                                                                                                                                                <  2:942121/150‑1 (MQ=255)
     ataAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCATACTTAAGTAGGGATAAAACGTTATTGCTATAATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGTGATGACTCTGGTCATc                                                                                                                                                >  1:291158/1‑150 (MQ=255)
       aaaaTCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACCAGAGTCATccc                                                                                                                                              <  2:549718/150‑1 (MQ=255)
       aaaaTCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACCAGAGTCATccc                                                                                                                                              <  1:1261751/150‑1 (MQ=255)
       aaaaTCTCATACCGGGAAATTAAAACAAGATGTACATTGAGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGGCTTATTATAGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATccc                                                                                                                                              <  2:493453/150‑1 (MQ=255)
        aaaTCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCt                                                                                                                                             >  2:71978/1‑150 (MQ=255)
        aaaTCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCt                                                                                                                                             >  1:1662289/1‑150 (MQ=255)
        aaaTCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACCAGAGTCATCCCt                                                                                                                                             >  1:1569287/1‑150 (MQ=255)
         aaTCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACCAGAGTCATCCCtg                                                                                                                                            <  2:1009731/150‑2 (MQ=255)
           tctcATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCa                                                                                                                                          <  2:728594/150‑1 (MQ=255)
             tcATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACCAGAGTCATCCCTGCaaa                                                                                                                                        <  2:1322024/150‑3 (MQ=255)
                  ccGGGACATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCAATCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTg                                                                                                                                   <  1:71247/150‑1 (MQ=255)
                  ccGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTg                                                                                                                                   <  1:1076682/150‑1 (MQ=255)
                  ccGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATATGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTg                                                                                                                                   <  2:755919/150‑1 (MQ=255)
                  ccGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAAACATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGTTCATCACTTCATCCCCTg                                                                                                                                   >  2:209330/1‑150 (MQ=255)
                      gAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGCGATTTGCAGGGATGAATCTGGTCATCCCTTCATCACCTGtata                                                                                                                               >  2:1021945/1‑150 (MQ=255)
                       aaaTTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAg                                                                                                                              <  1:1021513/150‑1 (MQ=255)
                          ttAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGta                                                                                                                           >  2:1385983/1‑150 (MQ=255)
                            aaaaGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCAGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCATTCATCCACTGTATAGGtaat                                                                                                                         >  2:1552915/1‑150 (MQ=255)
                                  agaTGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAAt                                                                                                                   >  1:1718466/1‑150 (MQ=255)
                                     tGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACt                                                                                                                >  1:1606604/1‑150 (MQ=255)
                                          aTTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTAc                                                                                                           >  2:1042614/1‑150 (MQ=255)
                                            tgtgCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCTGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTAccc                                                                                                         >  1:637869/1‑150 (MQ=255)
                                                cACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGcc                                                                                                    >  1:1125678/1‑150 (MQ=255)
                                                cACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGcc                                                                                                    >  2:600529/1‑150 (MQ=255)
                                                cACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGcc                                                                                                    >  2:1602256/1‑150 (MQ=255)
                                                 tttatCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCa                                                                                                   >  1:903878/5‑150 (MQ=255)
                                                 aCCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCa                                                                                                   >  2:344874/1‑150 (MQ=255)
                                                  ccTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAg                                                                                                  <  1:784650/150‑1 (MQ=255)
                                                  ccTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGACAg                                                                                                  >  2:1388862/1‑150 (MQ=255)
                                                    atCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGAc                                                                                                >  2:266516/2‑150 (MQ=255)
                                                    atCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGAc                                                                                                >  1:952182/2‑150 (MQ=255)
                                                      cccTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGAccc                                                                                              >  1:1028015/1‑150 (MQ=255)
                                                      cccTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTAATCCCTTCATCCCATGAATAGGTAATAACAATACTTTTACCCTGCCAGAccc                                                                                              >  1:1226925/1‑150 (MQ=255)
                                                         tACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACccatc                                                                                           <  1:11922/150‑1 (MQ=255)
                                                           cTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATATCAATACTGTTACCCTGCCAGACccatcca                                                                                         <  1:1401576/150‑1 (MQ=255)
                                                           cTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACccatcca                                                                                         <  2:1000830/150‑1 (MQ=255)
                                                           cTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACccatcca                                                                                         <  1:690305/150‑1 (MQ=255)
                                                             tAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCcatccatc                                                                                       >  2:1240192/1‑150 (MQ=255)
                                                               aGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGTTATTAACAAACAGTTTCCCATGCAAGACCAaaccaaccc                                                                                     >  2:847310/1‑149 (MQ=255)
                                                               aGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCAtccatcca                                                                                     <  1:1551849/150‑1 (MQ=255)
                                                                ttAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAg                                                                                    <  2:1179639/149‑1 (MQ=255)
                                                                gTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAg                                                                                    <  2:1662289/150‑1 (MQ=255)
                                                                    ggATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTc                                                                                <  1:344874/150‑1 (MQ=255)
                                                                    ggATAAAACGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTc                                                                                <  2:1604530/150‑1 (MQ=255)
                                                                      aTAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTg                                                                              >  2:1258568/1‑150 (MQ=255)
                                                                        aaaCCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGtt                                                                            >  1:655254/1‑150 (MQ=255)
                                                                         aaCCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGttt                                                                           >  1:500869/1‑150 (MQ=255)
                                                                         aaCCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTATGttt                                                                           >  2:339229/1‑150 (MQ=255)
                                                                          aCCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTc                                                                          >  2:916775/1‑150 (MQ=255)
                                                                           ccGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTcc                                                                         >  2:1624772/1‑150 (MQ=255)
                                                                            cGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACACATCCATCCAGATTCTGTTTccc                                                                        >  1:160425/1‑150 (MQ=255)
                                                                                aTTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTcc                                                                    <  1:605568/150‑1 (MQ=255)
                                                                                   ggTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGc                                                                 >  2:473650/1‑150 (MQ=255)
                                                                                    gTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGcc                                                                >  1:414306/1‑150 (MQ=255)
                                                                                    gTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGcc                                                                >  2:1653134/1‑150 (MQ=255)
                                                                                     tCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCa                                                               >  2:422109/1‑150 (MQ=255)
                                                                                      cTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCaa                                                              >  1:1199842/1‑150 (MQ=255)
                                                                                            atCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAAc                                                        <  1:769289/150‑1 (MQ=255)
                                                                                            atCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAAc                                                        <  1:840107/150‑1 (MQ=255)
                                                                                             tCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAAcc                                                       >  1:1577263/1‑150 (MQ=255)
                                                                                             tCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAAcc                                                       >  1:1524646/1‑150 (MQ=255)
                                                                                             tCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAAcc                                                       <  2:1086689/150‑1 (MQ=255)
                                                                                                 cATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATc                                                   >  1:424702/1‑150 (MQ=255)
                                                                                                   ttGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCtt                                                 <  1:219031/150‑1 (MQ=255)
                                                                                                      aTAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAGCAATACTGTTACCCTGCCAGACACATCCATCCAGATTCTGTTTCACTTCCAGCCAAAGTAACCCATCTTCAt                                              >  1:1423323/1‑150 (MQ=255)
                                                                                                       tAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATc                                             >  2:628473/1‑150 (MQ=255)
                                                                                                         aCAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATcta                                           <  1:1240192/150‑1 (MQ=255)
                                                                                                           aaTCATTCCCGAAGTTATTTGGGATTTGCAGGGATTACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATctact                                         <  2:55940/150‑1 (MQ=255)
                                                                                                            aTCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATctacta                                        <  1:732406/150‑1 (MQ=255)
                                                                                                              aaTTCCCGAATTTATTTTGGATTTGCATGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTactact                                      <  1:995350/149‑1 (MQ=255)
                                                                                                                ttCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTc                                    <  1:339096/150‑1 (MQ=255)
                                                                                                                 tCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCa                                   >  1:600243/1‑150 (MQ=255)
                                                                                                                 tCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCa                                   >  1:643245/1‑150 (MQ=255)
                                                                                                                  cccGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAg                                  <  2:1570845/150‑1 (MQ=255)
                                                                                                                      aaGTTATTTGGGATTTGCAGGGATGACTCTGGTCCTCCCTTCATCCCCTGTAAAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCAATCTTCAAATACTACTACAGaaaa                              >  1:1298649/1‑150 (MQ=255)
                                                                                                                        gTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAAc                            <  1:473650/150‑1 (MQ=255)
                                                                                                                          tATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTa                          <  1:101893/150‑1 (MQ=255)
                                                                                                                              tGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCCTCCCTCCAGATTCTGTTTCCCTTCCAGCCAAAGTCACCCCTCTTCCTCTACTACTTCAGAAAAACTACggt                      >  2:276591/1‑148 (MQ=255)
                                                                                                                               gggATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGttt                     <  1:1460956/150‑1 (MQ=255)
                                                                                                                                   tttGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAg                 <  1:745524/150‑1 (MQ=255)
                                                                                                                                    ttGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAgg                <  2:500869/150‑1 (MQ=255)
                                                                                                                                    ttGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAgg                <  2:109794/150‑1 (MQ=255)
                                                                                                                                    ttGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAgg                <  1:266516/150‑1 (MQ=255)
                                                                                                                                     tGCATGGATGACTCTGGTCCTCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTGCCCTTCCCGCCACAGTAACCCATCTTCATCTACTACTTCAGAAATACTACGTTTCCAGGt               <  1:276591/150‑1 (MQ=255)
                                                                                                                                     tGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGt               >  1:455723/1‑150 (MQ=255)
                                                                                                                                      gCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGCTTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTg              >  1:493192/1‑150 (MQ=255)
                                                                                                                                       cAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGAAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGa             >  2:225702/1‑150 (MQ=255)
                                                                                                                                        aGGGATGACCAGAGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACGTCAGAAAAACTACGGTTCCAGGTGaa            >  2:1400550/1‑150 (MQ=255)
                                                                                                                                          ggATGACTCTGGTCATCTCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAgg          >  2:386518/1‑150 (MQ=255)
                                                                                                                                              gACTCTGGTCATCCCTTCATCCCCTGTTTAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTAc      <  2:1076266/150‑1 (MQ=255)
                                                                                                                                              gACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTAc      <  1:758939/150‑1 (MQ=255)
                                                                                                                                               aCTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTTTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACa     <  1:1388862/150‑1 (MQ=255)
                                                                                                                                                ctctGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACAt    >  1:573395/1‑150 (MQ=255)
                                                                                                                                                  ctGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTg  <  1:139194/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                      
TGAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTG  >  NC_000913/2884432‑2884727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: